BupsA.01663.b

Hep_Hag family protein/haemagluttinin motif family protein/YadA-like domain protein

CENTER ID:	BupsA.01663.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01663.b.A1.GE26301	BupsA.01663.b.1 (Hep_Hag HIM 1)		376	549
BupsA.01663.b.A8.GE29403	head2_HaYHN domain		1095	1335

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01663.b.A1.PS00263	BupsA.01663.b.1 (Hep_Hag HIM 1)		376	549

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126579
PATRIC ID:	fig\|320372.6.peg.2256
RefSeq:	YP_333563.1
UniProt:	Q3JS90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKIFRVIWC RVKAACVVVS EEACLRGGKS HSCRQGSRAA GEESVRFALS SIALAACILI GSLGSTLPAV AGTVIGGGAQ YPNSVGGTSS TTGDLGNSYI GASGMGTAIT GDNDCLSLTS TRNVLNSANV GWLLGTTSQT TDPGPLYPGP GAENNQTISY SGTSNFSGGG NSAAGAQATL AWGFNSFAAG CGNKSLGMGS STLGLNNMAS LAGSTAIGIA NTASGAGGTA IGLYNTAAGT GSVAMGIGSQ ATGNGTIALG YGGGGTSSNA TLASGSNAIA IGGDATKGAQ ATGSDSIAMG SEAAASSSST TAIGQYATAS NTNATALGAG GTSAATGVIA SGAGAVALGG NSTQGAQALA SNAIAIGGQS QAASAGAIAI GQSALATGGQ AVSVGVGNTA NGNGAVAIGD PNVATGTGAV ALGANNTATG QGAVALGNAD IATGQGSVAL GNVSTAAGAG SVAFGSNAVA NNTNDVALGS GSVTAAPNPT GSATIGGTTY SFEGTNPTSV VSVGAVGAER QITNVAAGQL TATSTDAVNG SQLYSTNQAI NTLSTSTSTG LSSANSSIAS LSTGLASSGN LASLSTSTST GLSSANSSIA SLSTSTSTGL STTNSNIGSL STGLSTTNST VASLSTSTST GLSSATSSIT SLSTSTSSGI STAQSGVNSL STGLSTTNSA VTSLSTSTST GLSSATSSIT SLSTSTSTGI GSLSTGLSTT DSSVTSLSTS TSSGISTAQS GVNSLSTGLS TTNSTVTSLS TSASTGLSSA NSSITSLSTS TSTGLSSLST GIANSVQYDN TSHTQVTLGG AGATTPVTLT NVAAGVNPTD AVNMSQLTSL STSTSTGLST TNSNIGSLST ALSTTDSSVT SLSTSTSSGI STAQSGVNSL STGLSTTNST VASLSTSTST GLSSATSSIT SLSTSTSTGI GSLSTGLSTA NSSVTSLSTS TSSGISTAQS GVNSLSTGLS TTNSTVASLS TSTSTGLSSA TSSITSLSTS TSTGIGSLST GLSTANSSVT SLSTSTSSGI STAQSGVNSL STGLSTTNSA VTSLSTSTST GLSSLSTGLS ATNSNISSLS TSTSNGLSTA NSNISSLSTG LSSLSTAVNG GGTKYFHANS AQPDSQALGT NAIAVGPAAT ASGASGIAIG DTANAAATGA VAIGQTAVAT GGQAVSIGVA NTASGDGAVA IGDPNVATGT GAVALGANNS ANGQGAVALG NANVATGTGS LALGSTSTAA GGGSIALGTN AIANNANDVA LGSGSVTAAA NPVASALIAG QTYSLAGGSP TSVVSVGAPG AERQITNVAA GQVSATSTDA VNGSQMNAVT QALVSLSTST ANALSTTQNG LSSLSTGLST TQSSVSSLST GLSTTSGNVS SLSTGLSTTQ SDVASLSTGL STTNSNLASL STAVSNGGIH TNGAGGTSMG PGADASGSNS TAVGGAASAS GANATALGQA SNASGNNSTA LGQASSASGS GSTAVGQGAS ASGDGSSAFG QGAIASGTNS TALGAHSTAS APNSVAIGAN SVASAPNTVS FGSQGHERRL TNVAPGMDGT DAANMSQLWG VQSSVDQAAR RAYSGVAAAT ALTMIPEVDP GKTIAVGIGA GSYQGYSASA IGVSVRFSDN LKAKLGMGIS SQGSTYGGGI SYQW

NT Sequence

atgaataaaa tatttcgggt gatctggtgc cgtgtcaagg ctgcgtgcgt cgttgtttcg gaagaggcct gcctgcgcgg cggaaagagc catagctgcc ggcaaggcag ccgtgcggca ggtgaggaat ctgttcgttt cgcgttgtcg tccattgcgc tggcggcgtg catattgatc gggagccttg ggtccacgct gccggctgtg gctggtacgg taataggcgg cggggctcaa taccccaatt cggtaggcgg aacgagttct acgaccggcg atttgggcaa cagctatatt ggtgcgagcg gtatgggtac cgccattact ggggataatg attgcctgag cttgacttcg acccgcaacg tgctaaacag tgcgaatgtc ggctggcttt tgggaacgac ttcacagacc accgatcctg gtccgttgta ccccggcccc ggcgcggaaa acaaccagac tatcagttac agtggcacct ccaatttttc gggtggtgga aatagcgcgg ctggtgccca ggcgaccctc gcctggggct tcaattcctt cgcagcgggg tgtgggaaca aatcgcttgg catgggttcg agtacgctcg ggctcaataa catggccagc ttggccggtt caactgcgat cgggatcgct aataccgcgt caggagcggg cggaaccgcg atcggtctct acaacactgc cgctggcacg ggcagcgttg caatgggcat tggctctcaa gcgacaggca atggaacgat cgcactcgga tacggcggcg gcggcacctc gagcaacgcc acattggcga gcggcagcaa tgcgatcgcg ataggcggag acgcgaccaa aggcgcgcag gcgacgggaa gcgattcgat cgccatgggc agcgaggcgg ccgccagcag ttccagcacg accgcaatcg ggcaatatgc caccgctagc aatacgaatg ccacggcgct cggcgcgggc ggaacatccg ccgccactgg cgtaatcgca agcggtgcgg gcgccgtcgc gctcggtggc aacagtacgc aaggcgcgca ggcgttggca agcaacgcca ttgcgatcgg cggccaatcg caagccgcta gcgccggtgc gatcgcgatc ggccagagcg cactggccac gggtgggcaa gcggtgtccg tcggcgtggg caataccgcg aacggcaatg gcgcggtggc gatcggcgat ccgaacgtcg cgaccggcac gggagcggtg gcgctggggg cgaacaatac cgccaccgga caaggcgcgg ttgcgctcgg caacgctgat attgccacgg gccaaggttc ggttgcgctc ggtaatgtgt cgacggccgc cggtgcagga tcggtggcct tcggctcgaa tgccgtagcc aacaacacga acgatgtggc gctgggctcc ggatctgtga cagccgcgcc gaatccgacg gggagtgcga cgatcggcgg aaccacatat tcgtttgagg gaaccaatcc gacgagcgtt gtgagcgtgg gcgcagtggg cgcggaacgc cagatcacga acgtcgcggc gggacaactg acggcgacga gcacggacgc agtcaacggt tcgcaacttt actcgacgaa tcaggcgatt aatacattat cgacgtcgac ttcgacgggc ctgtcgtcgg cgaatagctc gatcgcgtcg ttatccactg gcctcgcatc gtccggcaac ctggcttcgc tgtcgacgtc cacgtcgaca ggcctgtcat cggcgaatag ctcgatcgcg tcgctgtcga cgtcgacttc aacgggcctc tcgacgacca acagcaacat cggctcgctg tcgacaggcc ttagcacgac taatagcacg gtcgcatcgt tatcgacgtc cacgtcgacg ggattgtcgt cggcgaccag ctcgatcacg tcgttgtcga cgtcgacctc gagcggcatc agtaccgctc agagcggcgt caattcgctg tcgactggcc tgtcgaccac caacagcgca gtcacctcgt tatcgacgtc cacgtcgacg ggattatcgt cggcgaccag ctcgatcacg tcgttgtcta cgtcgacgtc aactggaatc ggctcgctgt cgaccggtct gagcacgacc gacagttccg taacgtcgct gtcgacgtcg acctcgagcg gcatcagtac cgctcagagc ggcgtcaatt cgctgtcgac tggcctgtcg accaccaaca gcacggtcac ctcgttatcg acgtccgcgt cgacgggatt atcgtcggcg aacagctcga tcacgtcgtt gtcgacgtcg acctcgactg gattgagctc gttgtcgacg ggaatcgcga attccgttca gtatgacaac accagccata ctcaagtcac gttgggagga gccggcgcga cgacgccggt gacgctgacc aatgtcgccg cgggcgtgaa tcccaccgat gcggtgaata tgagtcagct gacttcgctg tcgacgtcaa cgtcaacggg actgtcgacg accaacagca acattggctc gctgtcgacc gctctgagca cgaccgacag ttccgtgacg tcgctgtcga cgtcgacctc gagcggcatc agtactgctc agagcggtgt caattcgctg tcgactggcc tgtcgaccac caacagcacg gtcgcatcgc tatcgacgtc cacgtcgacg ggattgtcgt cggcgaccag ctcgatcacg tcgttgtcta cgtcgacgtc aactggaatc ggctcgctgt cgaccggtct gagcacggcc aacagttccg tgacgtcgct gtcgacgtcg acctcgagcg gcatcagtac cgctcagagc ggcgtcaatt cgctgtcgac tggcctgtcg accaccaaca gcacggtcgc atcgctatcg acgtccacgt cgacgggatt gtcgtcggcg accagctcga tcacgtcgtt gtctacgtcg acgtcaactg gaatcggctc gctgtcgacc ggtctgagca cggccaacag ttccgtgacg tcgctgtcga cgtcgacctc gagcggcatc agtaccgctc agagcggcgt caattcgctg tcgactggcc tgtcgaccac caacagcgca gtcacttcgt tgtcgacgtc cacgtcgacg gggctgagct cgctgtccac gggtctgtcc gcgaccaaca gcaacatcag ttcgctgtcg acttcgacct cgaacggcct gagcacagct aacagcaata tctcgtcgct gtccactggg ctgagttcgc tgtcgaccgc agtcaacggc ggcgggacca agtactttca cgccaactcg gcgcagccgg acagtcaggc gctggggacg aacgcgatcg ccgtcggacc ggcggccacg gcatccggcg caagcggtat cgcgatcggc gataccgcga acgcggctgc aactggcgcg gtggcgattg gccagaccgc cgttgcaacg ggtggtcagg cggtctcgat cggtgttgcg aatacggcga gcggcgacgg cgcggtcgcg atcggtgatc cgaacgtcgc gacgggtact ggtgcggtcg cgctgggcgc gaacaattct gcgaacggcc agggcgccgt tgcgctcggc aatgcgaacg tggcaaccgg gacaggctcg ctggcgctcg gcagcacatc gacggcggcg gggggcggct cgatcgcgtt gggcacgaat gcaatcgcga acaatgccaa cgatgtcgcg ctgggttccg gtagcgtaac cgcagccgcg aatccggtcg ccagtgcgct gatcgccggt cagacgtatt cgctcgccgg cggttcaccg acaagcgtgg tgagtgtcgg tgcgccgggt gcagaacgac aaatcaccaa cgtcgcggca gggcaggttt ccgccacgtc gaccgacgcg gtgaacggat cgcagatgaa cgccgtgacc caagcgctgg tatcgttgtc gacttcgaca gccaatgcgt tgtcgacgac gcaaaacggt ctgagttcgt tgtcgacagg gctgagcacg acgcagagca gtgtgagttc gctgtcgacg ggccttagca cgacgagcgg taacgtgtcg tcgctgtcga ccggcttgag tacgacgcag agcgatgtcg cgtcgctgtc aacgggactg agtacgacga acagcaatct cgcgtcgttg tcgactgcgg tgtccaacgg agggattcac accaacggcg cgggcggcac gtcgatgggg ccgggagccg acgcgtcggg cagcaacagc acggcggtcg gtggcgcagc gtctgcttcg ggagccaatg caacggcgct tggccaggcg tcgaacgcat cgggcaataa ttcgacggca ttagggcaag catcgagtgc atccggaagc ggctccaccg cggtggggca gggcgcgagc gcgtcgggcg atgggtcctc ggcattcggc caaggggcga ttgcctccgg cacgaactcg acggcgctgg gcgctcattc gacggcatcg gcgccgaatt cagtggcgat cggtgcgaac tcggtagctt ccgcgccgaa cacggtgtcg ttcgggtctc agggccatga gcgtcgactg acgaacgtcg caccggggat ggacggtacc gacgcagcga acatgagcca gctttggggc gtgcaatcga gcgtagacca agccgcgcgc cgtgcgtatt cgggtgtggc ggctgccacc gcgctgacga tgattccgga agtcgatcct ggcaagacga ttgcagttgg gatcggtgcc ggcagctatc aagggtattc ggcatccgcg atcggtgtgt ccgtgcggtt ctccgacaac ctgaaggcga agctcggcat ggggatcagc agtcagggca gcacgtacgg cggaggcatc tcgtaccagt ggtaa

Details for BupsA.01663.b.A1.GE26301

HARVESTED ON:	3/10/2009
SEQUENCED ON:	3/26/2009
EXPECTED MW:	18kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGAIAIGQS ALATGGQAVS VGVGNTANGN GAVAIGDPNV ATGTGAVALG ANNTATGQGA VALGNADIAT GQGSVALGNV STAAGAGSVA FGSNAVANNT NDVALGSGSV TAAPNPTGSA TIGGTTYSFE GTNPTSVVSV GAVGAERQIT NVAAGQLTAT STDAVNGSQL YST

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtg cgatcgcgat cggccagagc gcactggcca cgggtgggca agcggtgtcc gtcggcgtgg gcaataccgc gaacggcaat ggcgcggtgg cgatcggcga tccgaacgtc gcgaccggca cgggagcggt ggcgctgggg gcgaacaata ccgccaccgg acaaggcgcg gttgcgctcg gcaacgctga tattgccacg ggccaaggtt cggttgcgct cggtaatgtg tcgacggccg ccggtgcagg atcggtggcc ttcggctcga atgccgtagc caacaacacg aacgatgtgg cgctgggctc cggatctgtg acagccgcgc cgaatccgac ggggagtgcg acgatcggcg gaaccacata ttcgtttgag ggaaccaatc cgacgagcgt tgtgagcgtg ggcgcagtgg gcgcggaacg ccagatcacg aacgtcgcgg cgggacaact gacggcgacg agcacggacg cagtcaacgg ttcgcaactt tactcgacga at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtgcgatc gcgatcggcc agagcgcact ggccacgggt gggcaagcgg tgtccgtcgg cgtgggcaat accgcgaacg gcaatggcgc ggtggcgatc ggcgatccga acgtcgcgac cggcacggga gcggtggcgc tgggggcgaa caataccgcc accggacaag gcgcggttgc gctcggcaac gctgatattg ccacgggcca aggttcggtt gcgctcggta atgtgtcgac ggccgccggt gcaggatcgg tggccttcgg ctcgaatgcc gtagccaaca acacgaacga tgtggcgctg ggctccggat ctgtgacagc cgcgccgaat ccgacgggga gtgcgacgat cggcggaacc acatattcgt ttgagggaac caatccgacg agcgttgtga gcgtgggcgc agtgggcgcg gaacgccaga tcacgaacgt cgcggcggga caactgacgg cgacgagcac ggacgcagtc aacggttcgc aactttactc gacgaatcag gcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BupsA.01663.b.A8.GE29403

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	24kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSSLSTGLS SLSTAVNGGG TKYFHANSAQ PDSQALGTNA IAVGPAATAS GASGIAIGDT ANAAATGAVA IGQTAVATGG QAVSIGVANT ASGDGAVAIG DPNVATGTGA VALGANNSAN GQGAVALGNA NVATGTGSLA LGSTSTAAGG GSIALGTNAI ANNANDVALG SGSVTAAANP VASALIAGQT YSLAGGSPTS VVSVGAPGAE RQITNVAAGQ VSATSTDAVN GSQMNAVTQA LVS

Validated NT Sequence

ttattacgat accagcgctt gggtcacggc gttcatctgc gatccgttca ccgcgtcggt cgacgtggcg gaaacctgcc ctgccgcgac gttggtgatt tgtcgttctg cacccggcgc accgacactc accacgcttg tcggtgaacc gccggcgagc gaatacgtct gaccggcgat cagcgcactg gcgaccggat tcgcggctgc ggttacgcta ccggaaccca gcgcgacatc gttggcattg ttcgcgattg cattcgtgcc caacgcgatc gagccgcccc ccgccgccgt cgatgtgctg ccgagcgcca gcgagcctgt cccggttgcc acgttcgcat tgccgagcgc aacggcgccc tggccgttcg cagaattgtt cgcgcccagc gcgaccgcac cagtacccgt cgcgacgttc ggatcaccga tcgcgaccgc gccgtcgccg ctcgccgtat tcgcaacacc gatcgagacc gcctgaccac ccgttgcaac ggcggtctgg ccaatcgcca ccgcgccagt tgcagccgcg ttcgcggtat cgccgatcgc gataccgctt gcgccggatg ccgtggccgc cggtccgacg gcgatcgcgt tcgtccccag cgcctgactg tccggctgcg ccgagttggc gtgaaagtac ttggtcccgc cgccgttgac tgcggtcgac agcgaactca gcccagtgga cagcgacgac atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtcgctg tccactgggc tgagttcgct gtcgaccgca gtcaacggcg gcgggaccaa gtactttcac gccaactcgg cgcagccgga cagtcaggcg ctggggacga acgcgatcgc cgtcggaccg gcggccacgg catccggcgc aagcggtatc gcgatcggcg ataccgcgaa cgcggctgca actggcgcgg tggcgattgg ccagaccgcc gttgcaacgg gtggtcaggc ggtctcgatc ggtgttgcga atacggcgag cggcgacggc gcggtcgcga tcggtgatcc gaacgtcgcg acgggtactg gtgcggtcgc gctgggcgcg aacaattctg cgaacggcca gggcgccgtt gcgctcggca atgcgaacgt ggcaaccggg acaggctcgc tggcgctcgg cagcacatcg acggcggcgg ggggcggctc gatcgcgttg ggcacgaatg caatcgcgaa caatgccaac gatgtcgcgc tgggttccgg tagcgtaacc gcagccgcga atccggtcgc cagtgcgctg atcgccggtc agacgtattc gctcgccggc ggttcaccga caagcgtggt gagtgtcggt gcgccgggtg cagaacgaca aatcaccaac gtcgcggcag ggcaggtttc cgccacgtcg accgacgcgg tgaacggatc gcagatgaac gccgtgaccc aagcgctggt atcgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BupsA.01663.b.A1.PS00263

PURIFICATION DATe:	4/24/2009
CONCENTRATION:	12.5mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTGCGATC GCGATCGGCC AGAGCGCACT GGCCACGGGT GGGCAAGCGG TGTCCGTCGG CGTGGGCAAT ACCGCGAACG GCAATGGCGC GGTGGCGATC GGCGATCCGA ACGTCGCGAC CGGCACGGGA GCGGTGGCGC TGGGGGCGAA CAATACCGCC ACCGGACAAG GCGCGGTTGC GCTCGGCAAC GCTGATATTG CCACGGGCCA AGGTTCGGTT GCGCTCGGTA ATGTGTCGAC GGCCGCCGGT GCAGGATCGG TGGCCTTCGG CTCGAATGCC GTAGCCAACA ACACGAACGA TGTGGCGCTG GGCTCCGGAT CTGTGACAGC CGCGCCGAAT CCGACGGGGA GTGCGACGAT CGGCGGAACC ACATATTCGT TTGAGGGAAC CAATCCGACG AGCGTTGTGA GCGTGGGCGC AGTGGGCGCG GAACGCCAGA TCACGAACGT CGCGGCGGGA CAACTGACGG CGACGAGCAC GGACGCAGTC AACGGTTCGC AACTTTACTC GACGAATCAG GCGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA