BupsA.01663.a
Hep_Hag family / Haemagluttinin family protein
| CENTER ID: | BupsA.01663.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.01663.a.A2.GE26299 | BupsA.01663.a.2 (Hep_Hag HIM 2) | 2278 | 2455 | ||
| BupsA.01663.a.A3.GE26300 | BupsA.01663.a.3 (Hep_Hag HIM 3) | 2482 | 2651 | ||
| BupsA.01663.a.A5.GE29396 | head1_YN domain | 107 | 254 | ||
| BupsA.01663.a.A6.GE29397 | head2_FHaYHN domain | 984 | 1158 | ||
| BupsA.01663.a.A7.GE29398 | head3_FHaYHN domain | 2281 | 2464 | ||
| BupsA.01663.a.A8.GE29399 | double head3_head4 domain | 2281 | 2695 | ||
| BupsA.01663.a.A9.GE29400 | head4_YNxshort domain | 2458 | 2669 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.01663.a.A2.PS00262 | BupsA.01663.a.2 (Hep_Hag HIM 2) | 2278 | 2455 |
Structures
3LAA
DEPOSITED: 1/6/2010
DETERMINATION: XRay
CLONE: BupsA.01663.a.A2.GE26299
PROTEIN: BupsA.01663.a.A2.PS00262
3LA9
DEPOSITED: 1/6/2010
DETERMINATION: XRay
CLONE: BupsA.01663.a.A2.GE26299
PROTEIN: BupsA.01663.a.A2.PS00262
Publications by SSGCID
Structure of a Burkholderia pseudomallei trimeric autotransporter adhesin head.
Abendroth J, Brittnacher MJ, Dieterich SH, Edwards TE, Guo W, Hewitt SN, Kelley A, Leibly D, Masoudi A, Miller SI, Myler PJ, Phan I, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2010
volume 5, issue 9, pages e12803
PMID: 20862217; PMCID: PMC2942831
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126579 |
| PATRIC ID: | fig|320372.6.peg.4474 |
| RefSeq: | YP_335617.1 |
| UniProt: | Q3JLD6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRSYRSIWN EALGAWVAAS EISSARGKPN KSAVAKVVTA AVLAVVVQAA HASTIGVGTG SLPGGSACGG IGGTGIGPDG TTNYTNRAIT DGSGFYSTVA GCSANGSNAL GVTLYGAFTT VTGDGGTAMG FAAYAAKWAS AVGLQASATG QSSSAFGFNA LASGANSLAL GSSSTAAYAN SVAIGQGSVT GAAAPTGTGY LTGSAAPSSE VSVGSSTNLR RITNVADGSA ANDAVTVAQL STGMSTTTSA IASLSTSTST GLSSARSSIA SLSTSTSTGL SSATSSIASL STSTSTGIGS LSTGLSTTNS NVASLSSGLS STNSSLTSLS TSASSGISTA QSGVNSLSTG LSTTNSTVAS LSTSTSTGLS SANSSIASLS TSASSGISTA QSGVNSLSTG LSTTNSTVAS LSTSTSTGLS SATSSIASLS TSTSTGIGSL STGLSTTNSN VASLSSGLSS TNSSLTSLST SASSGISTAQ SGVNSLSTGL STTNSTVASL STSTSTGLSS ATSSIASLST STSTGIGSLS TGLSTTNSNV ASLSSGLSST NSSLTSLSTS ASSGISTAQS GVNSLSTGLS TTNSTVASLS TSTSTGLSSA TSSIASLSTS TSTGIGSLSS GLSTTNSNVA SLSSGLSSTN SSLTSLSTSA SSGISTAQSG VNSLSTGLST ANSTVASLST STSTGLSSAT SSIASLSTST STGIGSLSTG LSTTNSNVAS LSSGLSSTNS SLTSLSTSAS SGISTAQSGV NSLSTGLSTT NSTVASLSTS TSTGLSSATS SIASLSTSTS TGIGSLSTGL STTNSNVASL SSGLSSTNSS LTSLSTSASS GISTAQSGVN SLSTGLSTAN STVASLSTST STGLSSATSS IASLSTSTST GIGSLSTGLS TTNSNVASLS SGLSSTNSSL TSLSTSASSG ISTAQSGVNS LSTGLSTTNS TVASLSTSTS TGLSSATSSI ASLSTSTSTG IGSLSTGLST VATTTNNLGS STAAALGGGA TYNPATGTIS APSYTTYNAN GTTTANTSVG SAIDSINANG IKYFHANSTG PDSVATGADA VAIGTGATAA TANSVALGAN SVTAAAVPTS SATVGSTTLG TFAGSAPVGV VSVGAPSAER QITNVAAGQV TASSTDAING SQLYAVASKL DSTSSSISSL STGLSSATSS ITSLSTSTST GLSSANSSIA SLSTSTSTGI GSLSTGLSTT DSAVASLSTS TSTGLSSATS SITSLSTSTS TGIGSLSTGL STTNSNVASL STSTSTGLSS ATSSIASLST STSTGIGSLS TGLSTTNSTV ASLSTSTSTG LSSANSSIAS LSTSTSTGIG SLSTGLSTTN SNVASLSSGL SSTNSSLTSL STSASSGIST AQSGVNSLST GLSTTNSTVA SLSTSTSTGL SSATSSIASL STSTSTGIGS LSTGLSTTDS TVASLSTSTS TGLSSATSSI ASLSTSTSTG IGSLSTGLST TNSNVASLSS GLSSTNSSLT SLSTSASSGI STAQSGVNSL STGLSTANST VASLSTSTST GLSSANSSIA SLSTSTSTGI GSLSTGLSTT DSTVASLSTS TSTGLSSANS SIASLSTSTS TGIGSLSTGL STTDSTVASL STSTSTGLSS ANSSIASLST STSTGIGSLS TGLSTTNSNV ASLSTSTSTG LSSANSSIAS LSTSTSTGIG SLSTGLSTTN SNVASLSTST STGLNLANSS IASLSTSTST SIDSLSTGLS TTNSNVASLS TSTSTGLSSA NSSIASLSTS TSTGIGSLST GLSTTNSNVA SLSTSTSTGL NLANSSIASL STSTSTGIGS LSTGLSTTNS NVASLSTSTS TGLSSATSSI ASLSTSTSTG IGSLSTGLST TDSTVASLST STSTGLSSAN SSIASLSTST STGIGSLSTG LSTTDSTVAS LSTSTSTGLS SATSSITSLS TSTSTGIGSL STGLSTTNSN VASLSTSTST GLSSATSSIA SLSTSTSTGI GSLSTGLSTT NSTVASLSTS TSTGLSSATS SIASLSTSTS TSIGSLSTGL STTDSTVASL STSTSTGLSS ANSSIASLST STSTGIGSLS TGLSTTDSTV ASLSTSTSTG LSSATSSIAS LSTSTSNGIG SLSTGLSTTN STVASLSTST STGLSSATSS IASLSTSTST GIGSLSTGLS TTNSTVASLS TSTSTGIGSL STGLSTTNST VASLSTSTST GIGSLSTGLS STNSNLVSLS TSASTGIGSL STSIDTTNSN LASLSTSSST GIGSLSTSIN ATNSNLASLS TSASTGIGSL STSISSITTN TTNLGNSTAA ALGGGATYDP ATGAISAPSY TTYNANGTTA TNTSVGAAID NINANGIKYF HANSTDPDSV ATGTNSVAIG PNAVANVDYS VAIGSGATTS AAVPVASASV GGLTFGGFAG SAPIGVFSVG APGAERQITN VAAGRISAAS TDAVNGSQLY ATNSNVASLS TGLNATNSNL ASLSTSTSTA VGSLSTGLST TNSTVASLST STSTSIGSLS TGLSTANSNL ASLSTSTSTG IGSLSTGLAT TNSNVASLST SVTNINTQLT SLSTSITNNV IRSLPASTGV AADMSAPKAT SPSVTAGSNS VALGAGSNDG GRSNVVSVGS DTQQRQITNV AAGTEGTDAV NVNQLNTLST SMSQSLSNQQ TQLNNLGSQL NQTQQQLQQT DTMARQGIAA VAAMASIPHM DRDSNFAMGV GTSSFLGQKA IAVGMQARIT ENLKASLNGG FAGNQKVIGA GMLYQWK
NT Sequence
atgaatcgct cgtaccgctc tatctggaat gaagcacttg gcgcgtgggt cgccgcttcg gaaatctcgt ccgcccgcgg caagccgaac aagtcggccg tcgcgaaggt cgtgaccgcc gccgtgctgg ccgtggtcgt gcaggcagcg catgcaagca cgatcggcgt cggaactggc tcgcttccgg gggggtccgc gtgcggcggc attggcggaa caggaatagg tcctgacggt acgacgaact atacgaaccg ggcgatcacc gacggcagcg gattctactc aaccgttgcc gggtgctcgg ccaacggcag caatgccctg ggcgtgacgc tctatggcgc attcacgacc gtcaccggag acggtggcac ggccatgggc ttcgccgctt acgccgcaaa gtgggcgtcg gccgttggcc ttcaggcgtc cgcgactggc cagtccagct cggcattcgg attcaatgcg ctggcgagcg gcgccaactc gcttgcactc ggctcgtcga gcaccgcggc gtacgcgaac agcgtcgcga tcggccaagg cagcgtcacg ggagcggcgg ccccgacggg gaccggctac ttgacgggca gcgccgcccc gtcttcggag gtgtccgtcg gttcctcgac caatttgcgc cgcatcacca acgttgcgga cggctccgca gcgaatgacg ccgtcacggt tgcccaactg agtacgggca tgagcacgac cactagcgca atcgccagtt tgtcgacctc cacatcgacc gggctttcgt cagccagaag ctcgatcgcg tcgctgtcca cgtcgacttc gaccggcctg agctcggcca ccagctcgat cgcttcactg tcgacttcca cgtcgaccgg catcggttcg ctttccaccg ggctgtcgac caccaacagc aacgtcgcct cgctgtccag cggcctgagc agcacgaaca gctcgctgac ctcgctgtcg acttccgcct cgtcgggcat cagcaccgcg cagagcggtg tcaattcgtt gtccaccggg ctgtcgacca ccaatagcac cgtcgcttcg ctgtccacgt cgacttcgac cggcttgagc tcggcgaata gctcgatcgc ctcgctttcg acttccgctt cgtcgggcat cagcaccgcg cagagcggcg tcaattcgtt gtccaccggg ctgtcgacca ccaatagcac cgtcgcttcg ctgtccacgt cgacctcgac cggcctgagc tcggccacca gctcgatcgc ttcgctgtcg acttccacgt cgaccggtat cggctcgctt tccaccggcc tgtcgaccac caacagcaac gtcgcctcgc tgtccagcgg cctgagcagc acgaatagct cgctgacctc gctgtcgacg tccgcttcgt cgggcatcag caccgcgcag agcggtgtca actcgttgtc caccgggctg tcgaccacca atagcaccgt cgcttcgctg tccacgtcga cttcgaccgg cctgagctcg gccaccagct cgatcgcttc gctgtcgacc tccacgtcga ccggcatcgg ctcgctttcc accggcctgt cgaccaccaa cagcaacgtc gcctcgctgt ccagcggact gagcagcacg aatagctcgc tgacctcgct gtcgacttcc gcctcgtctg gcatcagcac cgcgcagagc ggcgtcaatt cgttgtccac cgggctgtcg accaccaata gcaccgtcgc ttcgctgtcc acgtcgactt cgaccggctt gagctcggcc accagctcga tcgcttcgct gtcgacttcc acttcgaccg gtatcggttc gctttccagc ggcctgtcga ccaccaacag caacgtcgcc tcgctgtcca gcggcctgag cagcacgaat agctcgctga cctcgctgtc gacgtccgct tcgtcgggca tcagcaccgc gcagagcggt gtcaactcgt tgtccaccgg gctgtcgacc gcgaatagca ccgtcgcttc gctgtccacg tcgacttcga ccggcctgag ctcggccacc agctcgatcg cttcgctgtc gacttccacg tcgaccggta tcggctcgct ttccaccggc ctgtcgacca ccaacagcaa cgtcgcctcg ctgtccagcg gactgagcag cacgaatagc tcgctgacct cgctgtcgac ttccgcctcg tctggcatca gcaccgcgca gagcggcgtc aattcgttgt ccaccgggct gtcgaccacc aatagcaccg tcgcttcgct gtccacgtcg acctcgaccg gcctgagctc ggccaccagc tcgatcgctt cgctgtcgac ttccacgtcg accggtatcg gctcgctttc caccggcctg tcgaccacca acagcaacgt cgcctcgctg tccagcggcc tgagcagcac gaatagctcg ctgacctcgc tgtcgacgtc cgcttcgtcg ggcatcagca ccgcgcagag cggtgtcaac tcgttgtcca ccgggctgtc gaccgcgaat agcaccgtcg cttcgctgtc cacgtcgact tcgaccggcc tgagctcggc caccagctcg atcgcttcgc tgtcgacctc cacgtcgacc ggcatcggct cgctttccac cggcctgtcg accaccaaca gcaacgtcgc ctcgctgtcc agcggactga gcagcacgaa tagctcgctg acctcgctgt cgacttccgc ctcgtctggc atcagcaccg cgcagagcgg cgtcaattcg ttgtccaccg ggctgtcgac caccaatagc accgtcgctt cgctgtccac gtcgacttcg accggcttga gctcggccac cagctcgatc gcttcgctgt cgacttccac gtcgaccggc atcggctcgc tttccaccgg cctgtcgacc gtcgcgacga cgaccaacaa tctcggcagc agcaccgccg cggcgctcgg cggcggcgcg acgtacaatc cggccaccgg cacgatttcc gcgccgtcgt acacgacgta caacgcgaac ggcacgacca cggccaacac cagtgtcggc tctgcgatcg acagcatcaa cgcgaacggc atcaagtact tccatgcgaa ctcgactggg ccggacagcg tcgcgaccgg cgcggacgcc gtcgcgatcg gcacgggcgc gacggccgcc acggcgaact cggtcgcgct cggcgcgaac tcggtgacgg ccgcggccgt cccgaccagc agcgcgaccg tcggctcgac cacgctcggt acgttcgcgg gcagcgcgcc cgtgggcgtc gtgagcgtcg gggcgccgag cgcggaacgc cagatcacga acgtcgccgc cggccaggtc accgccagca gcaccgatgc gatcaacggc agccagctct atgcggttgc gtcgaagctc gactcgacct cgagttcgat ttcgtcgctg tcgacgggac tgtcgtcggc caccagttcg attacttcgc tctccacttc cacctcgacg ggcctgagct cggcgaatag ctcgatcgcc tcgctgtcga cctccacgtc gaccggtatc ggttcgctct ccaccggtct gtccacgacc gatagcgccg tcgcctcgct gtccacctcg acttcgaccg gcctgagctc ggccaccagc tcgatcactt cgctgtcgac ttccacgtcg accggcatcg gttcgctttc caccggcctg tccacaacca acagcaacgt cgcttcgctg tccacctcga cttcgaccgg tctgagctcg gccaccagct cgatcgcttc gctgtcgacc tccacgtcga ccggcatcgg ctcgctttcc accggtctgt ccacgaccaa tagcaccgtc gcctcgctgt ccacgtcgac ctcgaccggc ctgagctcgg cgaatagctc gatcgcctcg ctgtcgacct ccacgtcgac cggcatcggc tcgctttcca ccggcctgtc cacgaccaac agcaacgtcg cctcgctgtc cagcggcctg agcagcacga acagctcgct gacctcgctt tcgacttcag cttcgtccgg catcagcacc gcgcagagcg gtgtcaattc gttgtccacc gggctgtcga ccaccaatag caccgtcgct tcgctgtcca cctcgacctc gaccggcttg agctcggcca ccagctcgat cgcttcgctg tcgacttcca cgtcgaccgg catcggctcg ctctccaccg gtctgtccac gaccgatagc accgtcgcgt cgctgtccac gtcgacctcg accggcctga gttcggccac cagctcgatc gcttcgctgt cgacttccac gtcgaccggt atcggctcgc tttccaccgg cctgtcgacc accaacagca acgtcgcctc gctgtccagc ggcctgagca gcacgaatag ctcgctgacc tcgctgtcga cgtccgcttc gtcgggcatc agcaccgcgc agagcggcgt caattcgttg tccaccgggc tgtcgaccgc gaatagcacc gtcgcttcgc tgtccacgtc gacttcgacg ggcctgagct cggcgaacag ctcgatcgct tcgctgtcga cttccacgtc gaccggcatc ggctcgcttt ccaccggcct gtccacgacc gatagcaccg tcgcctcgct gtccacgtcg acttcgaccg gtctgagctc ggcgaatagc tcgatcgcct cgctgtcgac ctccacgtcg accggcatcg gctcgctttc caccggcctg tccacgaccg atagcaccgt cgcctcgctg tccacgtcga cttcgaccgg tctgagctcg gcgaatagct cgatcgcctc gctgtcgacc tccacgtcga ccggtatcgg ctcgctgtcc accggtctgt ccacgaccaa cagcaacgtc gcttcgctgt ccacctcgac ttcgacgggc ctgagctcgg cgaatagctc gatcgcctcg ctgtcgactt ccacgtcgac cggtatcggc tcgctttcca ccggcctgtc cacgaccaac agcaacgtcg cttcgctgtc cacctcgact tcgacgggcc tgaacttggc gaatagctcg atcgcctcgc tgtcgacttc cacgtcgacc agtatcgact cgctttccac cggcctgtcc acgaccaaca gcaacgtcgc ttcgctgtcc acctcgactt cgacgggcct gagctcggcg aatagctcga tcgcctcgct gtcgacttcc acgtcgaccg gtatcggctc gctttccacc ggcctgtcca cgaccaacag caacgtcgct tcgctgtcca cctcgacttc gacgggcctg aacttggcga atagctcgat cgcctcgctg tcgacctcca cgtcgaccgg tatcggctcg ctttccaccg gtctgtccac gaccaacagc aacgtcgctt cgctgtccac ctcgacttcg acgggtctga gctcggccac cagctcgatc gcttcgctgt cgacttccac gtcgaccggc atcggctcgc tctccaccgg tctgtccacg accgatagca ccgtcgcctc gctgtccacg tcgacttcga ccggcctgag ctcggcgaac agctcgatcg cctcgctgtc gacttccacg tcgaccggca tcggctcgct gtccaccggt ctgtccacga ccgatagcac cgtcgcctcg ctgtccacgt cgacttcgac cggcctgagc tcggccacca gctcgatcac ttcgctgtcg acttccacgt cgaccggcat cggttcgctt tccaccggcc tgtccacgac caacagcaac gtcgcttcgc tgtccacctc gacttcgacc ggtctgagct cggccaccag ctcgatcgct tcgctgtcga cctccacgtc gaccggcatc ggctcgcttt ccaccggtct gtcgacgacc aacagcaccg tcgcttcgct gtccacctcg acttcgacgg gcctgagctc ggccaccagc tcgatcgctt cgctgtcgac ttccacgtcg accagtatcg gctcgctttc caccggcctg tccacgaccg atagcaccgt cgcttcgctg tccacgtcga cttcgacggg cctgagctcg gcgaacagct cgatcgcttc gctgtcgact tccacgtcga ccggcatcgg ctcgctgtcc accggtctgt ccacgaccga tagcaccgtt gcttcgctgt ccacgtcgac ttcgaccggt ctgagctcgg ccaccagctc gatcgcttcg ctgtcgacct ccacgtcgaa cggtatcggc tcgctttcca ccggtctgtc cacgaccaac agcaccgtcg cttcgctgtc cacctcgact tcgacgggcc tgagctcggc caccagctcg atcgcctcgc tgtcgacctc cacgtcaacc ggcatcggct cgctttccac cggcctgtcc acgaccaaca gcaccgtcgc ctcgctgtcc acgtcgacct cgaccggcat cggctcgctc tccaccggcc tgtccacgac caacagcacc gtcgcctcgc tgtccacgtc gacttcgacc ggcatcggtt cgctgtccac cggcctgtcg tcgaccaaca gcaacctcgt gtcgctctcc acgtcggcct cgaccggcat cggctcgctg tcgaccagca tcgacacgac caacagcaac ctcgcgtcgc tctccacgtc gagctcgacc ggcatcggct cgctgtcgac cagcatcaac gcgaccaaca gcaacctcgc gtcgctctcc acgtccgcct cgaccggcat cggctcgctg tcgacgagca tcagctcgat cacgacgaac acgacgaacc tcggcaacag caccgccgcg gcgctcggcg gcggcgcgac gtacgatccg gccaccggcg cgatctccgc gccgtcgtac acgacgtaca acgcgaacgg caccaccgcg accaacacca gcgtcggcgc cgcgatcgac aacatcaacg cgaacggcat caagtacttc cacgcgaact cgaccgatcc ggacagcgtc gcgaccggca cgaacagcgt cgcgatcggc ccgaacgcgg tcgcgaacgt cgactactcg gtcgcgatcg gcagcggcgc gacgacctcg gcggccgtgc ccgtcgcgtc ggcgagcgtc ggcggcctca cgttcggcgg cttcgcgggc agcgcgccca tcggcgtgtt cagcgtcggc gcaccgggcg cggaacgcca gatcacgaat gtcgccgccg gccgcatctc cgcggccagc accgacgccg tcaacggcag ccagctctat gcgaccaaca gcaatgtcgc gtcgctgtcg accggtctga acgcgaccaa cagcaacctc gcgtcgctgt ccacgtccac ctcgaccgcc gtcggctcgc tgtccaccgg cctgtccacg accaacagca ccgtcgcctc gctgtccacg tcgacctcga ccagcatcgg ctcgctgtcc accggcctct cgaccgcgaa cagcaacctc gcgtcgctgt ccacgtccac ctcgaccggc atcggctcgc tgtccaccgg cctcgcgacg accaacagca atgtcgcgtc gctgtcgacg agcgtgacca acatcaacac gcagctcacg tcgctgtcga cgtcgatcac gaacaacgtg atccggtcgc tgcccgcgag caccggcgtc gccgcggaca tgagcgcgcc gaaggcgacc tcgccgtccg tcacggccgg ctcgaactcg gtcgcgctcg gcgcgggctc gaacgacggc ggtcgctcga acgtcgtgtc ggtgggcagc gacacgcagc agcgccagat cacgaacgtc gcggccggca ccgagggcac cgacgcggtc aacgtcaacc agttgaatac gctgtcgacg tcgatgtcgc aatcgctgtc gaatcagcaa acgcagctca acaatctcgg ctcgcaactg aaccagacgc agcagcaact gcagcagacc gacacgatgg cccgccaggg gatcgcggcg gtcgcggcga tggcgtcgat tccgcacatg gaccgcgact cgaacttcgc gatgggcgtg ggcacctctt cgttcctcgg ccagaaggcg atcgcggtcg gcatgcaggc gcgcatcacc gagaacctga aggcgtcgct gaacggcggc ttcgccggca atcagaaggt catcggcgcg ggcatgctct atcagtggaa gtaa
Details for BupsA.01663.a.A2.GE26299
| HARVESTED ON: | 3/10/2009 |
| SEQUENCED ON: | 3/26/2009 |
| EXPECTED MW: | 19kDa |
| OBSERVED MW: | 19kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGSLSTSIS SITTNTTNLG NSTAAALGGG ATYDPATGAI SAPSYTTYNA NGTTATNTSV GAAIDNINAN GIKYFHANST DPDSVATGTN SVAIGPNAVA NVDYSVAIGS GATTSAAVPV ASASVGGLTF GGFAGSAPIG VFSVGAPGAE RQITNVAAGR ISAASTDAVN GSQLYATNSN
Validated NT Sequence
ttaanaagga gnnnnaccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgggctcg ctgtcgacga gcatcagctc gatcacgacg aacacgacga acctcggcaa cagcaccgcc gcggcgctcg gcggcggcgc gacgtacgat ccggccaccg gcgcgatctc cgcgccgtcg tacacgacgt acaacgcgaa cggcaccacc gcgaccaaca ccagcgtcgg cgccgcgatc gacaacatca acgcgaacgg catcaagtac ttccacgcga actcgaccga tccggacagc gtcgcgaccg gcacgaacag cgtcgcgatc ggcccgaacg cggtcgcgaa cgtcgactac tcggtcgcga tcggcagcgg cgcgacgacc tcggcggccg tgcccgtcgc gtcggcgagc gtcggcggcc tcacgttcgg cggcttcgcg ggcagcgcgc ccatcggcgt gttcagcgtc ggcgcaccgg gcgcggaacg ccagatcacg aatgtcgccg ccggccgcat ctccgcggcc agcaccgacg ccgtcaacgg cagccagctc tatgcgacca acagcaatta ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSLSTSIS SITTNTTNLG NSTAAALGGG ATYDPATGAI SAPSYTTYNA NGTTATNTSV GAAIDNINAN GIKYFHANST DPDSVATGTN SVAIGPNAVA NVDYSVAIGS GATTSAAVPV ASASVGGLTF GGFAGSAPIG VFSVGAPGAE RQITNVAAGR ISAASTDAVN GSQLYATNSN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggctcgctg tcgacgagca tcagctcgat cacgacgaac acgacgaacc tcggcaacag caccgccgcg gcgctcggcg gcggcgcgac gtacgatccg gccaccggcg cgatctccgc gccgtcgtac acgacgtaca acgcgaacgg caccaccgcg accaacacca gcgtcggcgc cgcgatcgac aacatcaacg cgaacggcat caagtacttc cacgcgaact cgaccgatcc ggacagcgtc gcgaccggca cgaacagcgt cgcgatcggc ccgaacgcgg tcgcgaacgt cgactactcg gtcgcgatcg gcagcggcgc gacgacctcg gcggccgtgc ccgtcgcgtc ggcgagcgtc ggcggcctca cgttcggcgg cttcgcgggc agcgcgccca tcggcgtgtt cagcgtcggc gcaccgggcg cggaacgcca gatcacgaat gtcgccgccg gccgcatctc cgcggccagc accgacgccg tcaacggcag ccagctctat gcgaccaaca gcaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.01663.a.A3.GE26300
| HARVESTED ON: | 3/10/2009 |
| SEQUENCED ON: | 3/26/2009 |
| EXPECTED MW: | 18kDa |
| OBSERVED MW: | 19kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 38 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIAIGQS ALATGGQAVS VGVGNTANGN GAVAIGDPNV ATGTGAVALG ANNTATGQGA VALGNADIAT GQGSVALGNV STAAGAGSVA FGSNAVANNT NDVALGSGSV TAAPNPTGSA TIGGTTYSFE GTNPTSVVSV GAVGAERQIT NVAAGQLTAT STDAVNGSQL YSTNQA
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSLSTGLS TTNSTVASLS TSTSTSIGSL STGLSTANSN LASLSTSTST GIGSLSTGLA TTNSNVASLS TSVTNINTQL TSLSTSITNN VIRSLPASTG VAADMSAPKA TSPSVTAGSN SVALGAGSND GGRSNVVSVG SDTQQRQITN VAAGTEGTDA VNVNQLNTLS TS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggctcgctg tccaccggcc tgtccacgac caacagcacc gtcgcctcgc tgtccacgtc gacctcgacc agcatcggct cgctgtccac cggcctctcg accgcgaaca gcaacctcgc gtcgctgtcc acgtccacct cgaccggcat cggctcgctg tccaccggcc tcgcgacgac caacagcaat gtcgcgtcgc tgtcgacgag cgtgaccaac atcaacacgc agctcacgtc gctgtcgacg tcgatcacga acaacgtgat ccggtcgctg cccgcgagca ccggcgtcgc cgcggacatg agcgcgccga aggcgacctc gccgtccgtc acggccggct cgaactcggt cgcgctcggc gcgggctcga acgacggcgg tcgctcgaac gtcgtgtcgg tgggcagcga cacgcagcag cgccagatca cgaacgtcgc ggccggcacc gagggcaccg acgcggtcaa cgtcaaccag ttgaatacgc tgtcgacgtc gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BupsA.01663.a.A5.GE29396
| HARVESTED ON: | 5/27/2010 |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 16kDa |
| OBSERVED MW: | 16kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNALGVTL YGAFTTVTGD GGTAMGFAAY AAKWASAVGL QASATGQSSS AFGFNALASG ANSLALGSSS TAAYANSVAI GQGSVTGAAA PTGTGYLTGS AAPSSEVSVG SSTNLRRITN VADGSAANDA VTVAQLSTGM STTTSAIASL
Validated NT Sequence
ttttgtnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcaa tgccctgggc gtgacgctct atggcgcatt cacgaccgtc accggagacg gtggcacggc catgggcttc gccgcttacg ccgcaaagtg ggcgtcggcc gttggccttc aggcgtccgc gactggccag tccagctcgg cattcggatt caatgcgctg gcgagcggcg ccaactcgct tgcactcggc tcgtcgagca ccgcggcgta cgcgaacagc gtcgcgatcg gccaaggcag cgtcacggga gcggcggccc cgacggggac cggctacttg acgggcagcg ccgccccgtc ttcggaggtg tccgtcggtt cctcgaccaa tttgcgccgc atcaccaacg ttgcggacgg ctccgcagcg aatgacgccg tcacggttgc ccaactgagt acgggcatga gcacgaccac tagcgcaatc gccagtttgt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca nnnnnctagc a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNALGVTL YGAFTTVTGD GGTAMGFAAY AAKWASAVGL QASATGQSSS AFGFNALASG ANSLALGSSS TAAYANSVAI GQGSVTGAAA PTGTGYLTGS AAPSSEVSVG SSTNLRRITN VADGSAANDA VTVAQLSTGM STTTSAIASL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaatgcc ctgggcgtga cgctctatgg cgcattcacg accgtcaccg gagacggtgg cacggccatg ggcttcgccg cttacgccgc aaagtgggcg tcggccgttg gccttcaggc gtccgcgact ggccagtcca gctcggcatt cggattcaat gcgctggcga gcggcgccaa ctcgcttgca ctcggctcgt cgagcaccgc ggcgtacgcg aacagcgtcg cgatcggcca aggcagcgtc acgggagcgg cggccccgac ggggaccggc tacttgacgg gcagcgccgc cccgtcttcg gaggtgtccg tcggttcctc gaccaatttg cgccgcatca ccaacgttgc ggacggctcc gcagcgaatg acgccgtcac ggttgcccaa ctgagtacgg gcatgagcac gaccactagc gcaatcgcca gtttgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.01663.a.A6.GE29397
| HARVESTED ON: | 5/27/2010 |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 18kDa |
| OBSERVED MW: | 20kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 88 |
Validated AA Sequence
MTTNNLGSST AAALGGGATY NPATGTISAP SYTTYNANGT TTANTXVGSA IDSINANGIK YFHANSTGPD SXATGAXAVA IGTGXTAATA NSXALGANSV TAAAVPXSSA TVGSTTLGTF AGSAPVGVVS VGAPSAERQI TNVAAGQVTA SSTDAINGSQ LYAVASKLDS TSSSI
Validated NT Sequence
gaaatcgaac tcgaggtcga gtcgagcttc gacgcaaccg catagagctg gctgccgttg atcgcatcgg tgctgctggc ggtgacctgg ccggcggcga cgttcgtgat ctggcgttcc gcgctcggcg ccccgacgct cacgacgccc acgggcgcgc tgcccgcgaa cgtaccgagc gtggtcgagc cgacggtcgc gctgctggnc gggacggccg cggccgtcac cgagttcgcg ccgagcgcgn ccgagttcgc cgtggcggcn gtngnncccg tgccgatcgc gacggcgtnc gcgccggtcg cgncgctgtc cggcccagtc gagttcgcat ggaagtactt gatgccgttc gcgttgatgc tgtcgatcgc agagccgacn cnggtgttgg ccgtggtcgt gccgttcgcg ttgtacgtcg tgtacgacgg cgcggaaatc gtgccggtgg ccggattgta cgtcgcgccg ccnccgagcg ccgcggcggt gctgctgccg agattgttgg tcgtcatcga accaggaccc tgggtctgag cttccanng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTNNLGSS TAAALGGGAT YNPATGTISA PSYTTYNANG TTTANTSVGS AIDSINANGI KYFHANSTGP DSVATGADAV AIGTGATAAT ANSVALGANS VTAAAVPTSS ATVGSTTLGT FAGSAPVGVV SVGAPSAERQ ITNVAAGQVT ASSTDAINGS QLYAVASKLD STSSSIS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgaccaac aatctcggca gcagcaccgc cgcggcgctc ggcggcggcg cgacgtacaa tccggccacc ggcacgattt ccgcgccgtc gtacacgacg tacaacgcga acggcacgac cacggccaac accagtgtcg gctctgcgat cgacagcatc aacgcgaacg gcatcaagta cttccatgcg aactcgactg ggccggacag cgtcgcgacc ggcgcggacg ccgtcgcgat cggcacgggc gcgacggccg ccacggcgaa ctcggtcgcg ctcggcgcga actcggtgac ggccgcggcc gtcccgacca gcagcgcgac cgtcggctcg accacgctcg gtacgttcgc gggcagcgcg cccgtgggcg tcgtgagcgt cggggcgccg agcgcggaac gccagatcac gaacgtcgcc gccggccagg tcaccgccag cagcaccgat gcgatcaacg gcagccagct ctatgcggtt gcgtcgaagc tcgactcgac ctcgagttcg atttcgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.01663.a.A7.GE29398
| HARVESTED ON: | 5/27/2010 |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 19kDa |
| OBSERVED MW: | 21kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSISSIT TNTTNLGNST AAALGGGATY DPATGAISAP SYTTYNANGT TATNTSVGAA IDNINANGIK YFHANSTDPD SVATGTNSVA IGPNAVANVD YSVAIGSGAT TSAAVPVASA SVGGLTFGGF AGSAPIGVFS XXAPGAERXX XNVAAGRISA ASTDAVNGSQ LYATNSNVAS LXTGLN
Validated NT Sequence
annnnnnngt ttactttaag aaggaannta ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgtc gacgagcatc agctcgatca cgacgaacac gacgaacctc ggcaacagca ccgccgcggc gctcggcggc ggcgcgacgt acgatccggc caccggcgcg atctccgcgc cgtcgtacac gacgtacaac gcgaacggca ccaccgcgac caacaccagc gtcggcgccg cgatcgacaa catcaacgcg aacggcatca agtacttcca cgcgaactcg accgatccgg acagcgtcgc gaccggcacg aacagcgtcg cgatcggccc gaacgcggtc gcgaacgtcg actactcggt cgcgatcggc agcggcgcga cgacctcggc ggccgtgccc gtcgcgtcgg cgagcgtcgg cggcctcacg ttcggcggct tcgcgggcag cgcgcccatc ggcgtgttca gcgncngcgc accgggngcg gaacgccnna ncnngaatgt cgccgccggc cgcatctccg cggccagcac cgacgccgtc aacggcagcc agctctatgc gaccaacagc aatgtcgcgt cgctgncgac cggnctgaac taataaacag cacgaacaag ttctgcagcc aagctttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgan cannnnctag ca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSISSIT TNTTNLGNST AAALGGGATY DPATGAISAP SYTTYNANGT TATNTSVGAA IDNINANGIK YFHANSTDPD SVATGTNSVA IGPNAVANVD YSVAIGSGAT TSAAVPVASA SVGGLTFGGF AGSAPIGVFS VGAPGAERQI TNVAAGRISA ASTDAVNGSQ LYATNSNVAS LSTGLN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgacgagc atcagctcga tcacgacgaa cacgacgaac ctcggcaaca gcaccgccgc ggcgctcggc ggcggcgcga cgtacgatcc ggccaccggc gcgatctccg cgccgtcgta cacgacgtac aacgcgaacg gcaccaccgc gaccaacacc agcgtcggcg ccgcgatcga caacatcaac gcgaacggca tcaagtactt ccacgcgaac tcgaccgatc cggacagcgt cgcgaccggc acgaacagcg tcgcgatcgg cccgaacgcg gtcgcgaacg tcgactactc ggtcgcgatc ggcagcggcg cgacgacctc ggcggccgtg cccgtcgcgt cggcgagcgt cggcggcctc acgttcggcg gcttcgcggg cagcgcgccc atcggcgtgt tcagcgtcgg cgcaccgggc gcggaacgcc agatcacgaa tgtcgccgcc ggccgcatct ccgcggccag caccgacgcc gtcaacggca gccagctcta tgcgaccaac agcaatgtcg cgtcgctgtc gaccggtctg aacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BupsA.01663.a.A8.GE29399
| HARVESTED ON: | 5/27/2010 |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 74 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSISSIT TNTTNLGNST AAALGGGATY DPATGAISAP SYTTYNANGT TATNTSVGAA IDNINANGIK YFHANSTDPD SVATGTNSVA IGPNAVANVD YSVAIGSGAT TSAAVPVASA SVGGLTFGGF AGSAPIGVFS VGAPGAERQI TNVAAGRISA ASTDAVNGSQ LYATNSNVAS LSTGLNATNS NLASLSTSTS TAVGSLSTGL STTNSTVASL STSXSTSIGS LSTGLSTANS XLASLSTSTS TGIGSLSTGL ATTNSXVASL STSXXXHQHA AHVAVDVDHX QRDPVAARXX RASPXX
Validated NT Sequence
tttntttanc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtcgac gagcatcagc tcgatcacga cgaacacgac gaacctcggc aacagcaccg ccgcggcgct cggcggcggc gcgacgtacg atccggccac cggcgcgatc tccgcgccgt cgtacacgac gtacaacgcg aacggcacca ccgcgaccaa caccagcgtc ggcgccgcga tcgacaacat caacgcgaac ggcatcaagt acttccacgc gaactcgacc gatccggaca gcgtcgcgac cggcacgaac agcgtcgcga tcggcccgaa cgcggtcgcg aacgtcgact actcggtcgc gatcggcagc ggcgcgacga cctcggcggc cgtgcccgtc gcgtcggcga gcgtcggcgg cctcacgttc ggcggcttcg cgggcagcgc gcccatcggc gtgttcagcg tcggcgcacc gggcgcggaa cgccagatca cgaatgtcgc cgccggccgc atctccgcgg ccagcaccga cgccgtcaac ggcagccagc tctatgcgac caacagcaat gtcgcgtcgc tgtcgaccgg tctgaacgcg accaacagca acctcgcgtc gctgtccacg tccacctcga ccgccgtcgg ctcgctgtcc accggcctgt ccacgaccaa cagcaccgtc gcctcgctgt ccacgtcgan ctcgaccagc atcggctcgc tgtccaccgg cctctcgacc gcgaacagca ncctcgcgtc gctgtccacg tccacctcga ccggcatcgg ctcgctgtcc accggcctcg cgacgaccaa cagcnatgtc gcgtcgctgt cgacgagcgn gancnnacat caacacgcag ctcacgtcgc tgtcgacgtc gatcacnaac aacgtgatcc ggtcgctgcc cgcgnnnacc gggcgtcncc nnnganatga nnnngcnnaa ggngactcgn cnnncngnnn cnggccggnt cgaactcngn cnngctnggn nnngggntcn aacnangnnn gnngntnnan nnnnnnnnng gngggnnncn nnnancgccn gnnnnnnnna angtnnnnnn ngnnncnnnn ggnnnngnnn nnnnnnnnnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSISSIT TNTTNLGNST AAALGGGATY DPATGAISAP SYTTYNANGT TATNTSVGAA IDNINANGIK YFHANSTDPD SVATGTNSVA IGPNAVANVD YSVAIGSGAT TSAAVPVASA SVGGLTFGGF AGSAPIGVFS VGAPGAERQI TNVAAGRISA ASTDAVNGSQ LYATNSNVAS LSTGLNATNS NLASLSTSTS TAVGSLSTGL STTNSTVASL STSTSTSIGS LSTGLSTANS NLASLSTSTS TGIGSLSTGL ATTNSNVASL STSVTNINTQ LTSLSTSITN NVIRSLPAST GVAADMSAPK ATSPSVTAGS NSVALGAGSN DGGRSNVVSV GSDTQQRQIT NVAAGTEGTD AVNVNQLNTL STSMSQSLSN QQTQLNNLGS QLNQTQQQLQ QTDTMARQGI AAVAAMA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgacgagc atcagctcga tcacgacgaa cacgacgaac ctcggcaaca gcaccgccgc ggcgctcggc ggcggcgcga cgtacgatcc ggccaccggc gcgatctccg cgccgtcgta cacgacgtac aacgcgaacg gcaccaccgc gaccaacacc agcgtcggcg ccgcgatcga caacatcaac gcgaacggca tcaagtactt ccacgcgaac tcgaccgatc cggacagcgt cgcgaccggc acgaacagcg tcgcgatcgg cccgaacgcg gtcgcgaacg tcgactactc ggtcgcgatc ggcagcggcg cgacgacctc ggcggccgtg cccgtcgcgt cggcgagcgt cggcggcctc acgttcggcg gcttcgcggg cagcgcgccc atcggcgtgt tcagcgtcgg cgcaccgggc gcggaacgcc agatcacgaa tgtcgccgcc ggccgcatct ccgcggccag caccgacgcc gtcaacggca gccagctcta tgcgaccaac agcaatgtcg cgtcgctgtc gaccggtctg aacgcgacca acagcaacct cgcgtcgctg tccacgtcca cctcgaccgc cgtcggctcg ctgtccaccg gcctgtccac gaccaacagc accgtcgcct cgctgtccac gtcgacctcg accagcatcg gctcgctgtc caccggcctc tcgaccgcga acagcaacct cgcgtcgctg tccacgtcca cctcgaccgg catcggctcg ctgtccaccg gcctcgcgac gaccaacagc aatgtcgcgt cgctgtcgac gagcgtgacc aacatcaaca cgcagctcac gtcgctgtcg acgtcgatca cgaacaacgt gatccggtcg ctgcccgcga gcaccggcgt cgccgcggac atgagcgcgc cgaaggcgac ctcgccgtcc gtcacggccg gctcgaactc ggtcgcgctc ggcgcgggct cgaacgacgg cggtcgctcg aacgtcgtgt cggtgggcag cgacacgcag cagcgccaga tcacgaacgt cgcggccggc accgagggca ccgacgcggt caacgtcaac cagttgaata cgctgtcgac gtcgatgtcg caatcgctgt cgaatcagca aacgcagctc aacaatctcg gctcgcaact gaaccagacg cagcagcaac tgcagcagac cgacacgatg gcccgccagg ggatcgcggc ggtcgcggcg atggcgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.01663.a.A9.GE29400
| HARVESTED ON: | 5/27/2010 |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 23kDa |
| OBSERVED MW: | 23kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLSTGLNA TNSNLASLST STSTAVGSLS TGLSTTNSTV ASLSTSTSTS IGSLSTGLST ANSNLASLST STSTGIGSLS TGLATTNSNV ASLSTSVTNI NTQLTSLSTS ITNNVIRSLP ASTGVAADMS APKATSPSVT AGSNSVALGA GSNDGGRSNV VSVGSDTQQR QITNVAAGTE GTDAVNVNQL NTLSTSMSQS LSNQQTQLNN LGSQ
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtcgct gtcgaccggt ctgaacgcga ccaacagcaa cctcgcgtcg ctgtccacgt ccacctcgac cgccgtcggc tcgctgtcca ccggcctgtc cacgaccaac agcaccgtcg cctcgctgtc cacgtcgacc tcgaccagca tcggctcgct gtccaccggc ctctcgaccg cgaacagcaa cctcgcgtcg ctgtccacgt ccacctcgac cggcatcggc tcgctgtcca ccggcctcgc gacgaccaac agcaatgtcg cgtcgctgtc gacgagcgtg accaacatca acacgcagct cacgtcgctg tcgacgtcga tcacgaacaa cgtgatccgg tcgctgcccg cgagcaccgg cgtcgccgcg gacatgagcg cgccgaaggc gacctcgccg tccgtcacgg ccggctcgaa ctcggtcgcg ctcggcgcgg gctcgaacga cggcggtcgc tcgaacgtcg tgtcggtggg cagcgacacg cagcagcgcc agatcacgaa cgtcgcggcc ggcaccgagg gcaccgacgc ggtcaacgtc aaccagttga atacgctgtc gacgtcgatg tcgcaatcgc tgtcgaatca gcaaacgcag ctcaacaatc tcggctcgca ataataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgan cnannnacta gca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLSTGLNA TNSNLASLST STSTAVGSLS TGLSTTNSTV ASLSTSTSTS IGSLSTGLST ANSNLASLST STSTGIGSLS TGLATTNSNV ASLSTSVTNI NTQLTSLSTS ITNNVIRSLP ASTGVAADMS APKATSPSVT AGSNSVALGA GSNDGGRSNV VSVGSDTQQR QITNVAAGTE GTDAVNVNQL NTLSTSMSQS LSNQQTQLNN LGSQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgctgtcg accggtctga acgcgaccaa cagcaacctc gcgtcgctgt ccacgtccac ctcgaccgcc gtcggctcgc tgtccaccgg cctgtccacg accaacagca ccgtcgcctc gctgtccacg tcgacctcga ccagcatcgg ctcgctgtcc accggcctct cgaccgcgaa cagcaacctc gcgtcgctgt ccacgtccac ctcgaccggc atcggctcgc tgtccaccgg cctcgcgacg accaacagca atgtcgcgtc gctgtcgacg agcgtgacca acatcaacac gcagctcacg tcgctgtcga cgtcgatcac gaacaacgtg atccggtcgc tgcccgcgag caccggcgtc gccgcggaca tgagcgcgcc gaaggcgacc tcgccgtccg tcacggccgg ctcgaactcg gtcgcgctcg gcgcgggctc gaacgacggc ggtcgctcga acgtcgtgtc ggtgggcagc gacacgcagc agcgccagat cacgaacgtc gcggccggca ccgagggcac cgacgcggtc aacgtcaacc agttgaatac gctgtcgacg tcgatgtcgc aatcgctgtc gaatcagcaa acgcagctca acaatctcgg ctcgcaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BupsA.01663.a.A2.PS00262
| PURIFICATION DATe: | 4/22/2009 |
| CONCENTRATION: | 24.89mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGSLSTSIS SITTNTTNLG NSTAAALGGG ATYDPATGAI SAPSYTTYNA NGTTATNTSV GAAIDNINAN GIKYFHANST DPDSVATGTN SVAIGPNAVA NVDYSVAIGS GATTSAAVPV ASASVGGLTF GGFAGSAPIG VFSVGAPGAE RQITNVAAGR ISAASTDAVN GSQLYATNSN
Validated NT Sequence
ttaanaagga gnnnnaccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgggctcg ctgtcgacga gcatcagctc gatcacgacg aacacgacga acctcggcaa cagcaccgcc gcggcgctcg gcggcggcgc gacgtacgat ccggccaccg gcgcgatctc cgcgccgtcg tacacgacgt acaacgcgaa cggcaccacc gcgaccaaca ccagcgtcgg cgccgcgatc gacaacatca acgcgaacgg catcaagtac ttccacgcga actcgaccga tccggacagc gtcgcgaccg gcacgaacag cgtcgcgatc ggcccgaacg cggtcgcgaa cgtcgactac tcggtcgcga tcggcagcgg cgcgacgacc tcggcggccg tgcccgtcgc gtcggcgagc gtcggcggcc tcacgttcgg cggcttcgcg ggcagcgcgc ccatcggcgt gttcagcgtc ggcgcaccgg gcgcggaacg ccagatcacg aatgtcgccg ccggccgcat ctccgcggcc agcaccgacg ccgtcaacgg cagccagctc tatgcgacca acagcaatta ataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSLSTSIS SITTNTTNLG NSTAAALGGG ATYDPATGAI SAPSYTTYNA NGTTATNTSV GAAIDNINAN GIKYFHANST DPDSVATGTN SVAIGPNAVA NVDYSVAIGS GATTSAAVPV ASASVGGLTF GGFAGSAPIG VFSVGAPGAE RQITNVAAGR ISAASTDAVN GSQLYATNSN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCTCGCTG TCGACGAGCA TCAGCTCGAT CACGACGAAC ACGACGAACC TCGGCAACAG CACCGCCGCG GCGCTCGGCG GCGGCGCGAC GTACGATCCG GCCACCGGCG CGATCTCCGC GCCGTCGTAC ACGACGTACA ACGCGAACGG CACCACCGCG ACCAACACCA GCGTCGGCGC CGCGATCGAC AACATCAACG CGAACGGCAT CAAGTACTTC CACGCGAACT CGACCGATCC GGACAGCGTC GCGACCGGCA CGAACAGCGT CGCGATCGGC CCGAACGCGG TCGCGAACGT CGACTACTCG GTCGCGATCG GCAGCGGCGC GACGACCTCG GCGGCCGTGC CCGTCGCGTC GGCGAGCGTC GGCGGCCTCA CGTTCGGCGG CTTCGCGGGC AGCGCGCCCA TCGGCGTGTT CAGCGTCGGC GCACCGGGCG CGGAACGCCA GATCACGAAT GTCGCCGCCG GCCGCATCTC CGCGGCCAGC ACCGACGCCG TCAACGGCAG CCAGCTCTAT GCGACCAACA GCAATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA