BupsA.01663.a
Hep_Hag family / Haemagluttinin family protein
CENTER ID:
BupsA.01663.a
ORGANISM:
Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:
Melioidosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
BupsA.01663.a.A2.GE26299
BupsA.01663.a.2 (Hep_Hag HIM 2)
info
2278
2455
BupsA.01663.a.A2.GE26299
BupsA.01663.a.A3.GE26300
BupsA.01663.a.3 (Hep_Hag HIM 3)
info
2482
2651
BupsA.01663.a.A3.GE26300
BupsA.01663.a.A5.GE29396
head1_YN domain
info
107
254
BupsA.01663.a.A5.GE29396
BupsA.01663.a.A6.GE29397
head2_FHaYHN domain
info
984
1158
BupsA.01663.a.A6.GE29397
BupsA.01663.a.A7.GE29398
head3_FHaYHN domain
info
2281
2464
BupsA.01663.a.A7.GE29398
BupsA.01663.a.A8.GE29399
double head3_head4 domain
info
2281
2695
BupsA.01663.a.A8.GE29399
BupsA.01663.a.A9.GE29400
head4_YNxshort domain
info
2458
2669
BupsA.01663.a.A9.GE29400
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BupsA.01663.a.A2.PS00262
BupsA.01663.a.2 (Hep_Hag HIM 2)
info
2278
2455
PS00262
Structures
3LAA
DEPOSITED: 1/6/2010
DETERMINATION: XRay
CLONE: BupsA.01663.a.A2.GE26299
PROTEIN: BupsA.01663.a.A2.PS00262
3LA9
DEPOSITED: 1/6/2010
DETERMINATION: XRay
CLONE: BupsA.01663.a.A2.GE26299
PROTEIN: BupsA.01663.a.A2.PS00262
Publications by SSGCID
Structure of a Burkholderia pseudomallei trimeric autotransporter adhesin head.
Abendroth J, Brittnacher MJ, Dieterich SH, Edwards TE, Guo W, Hewitt SN, Kelley A, Leibly D, Masoudi A, Miller SI, Myler PJ, Phan I, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2010
volume 5, issue 9, pages e12803
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRSYRSIWN EALGAWVAAS EISSARGKPN KSAVAKVVTA AVLAVVVQAA HASTIGVGTG SLPGGSACGG IGGTGIGPDG TTNYTNRAIT DGSGFYSTVA GCSANGSNAL GVTLYGAFTT VTGDGGTAMG FAAYAAKWAS AVGLQASATG QSSSAFGFNA LASGANSLAL GSSSTAAYAN SVAIGQGSVT GAAAPTGTGY LTGSAAPSSE VSVGSSTNLR RITNVADGSA ANDAVTVAQL STGMSTTTSA IASLSTSTST GLSSARSSIA SLSTSTSTGL SSATSSIASL STSTSTGIGS LSTGLSTTNS NVASLSSGLS STNSSLTSLS TSASSGISTA QSGVNSLSTG LSTTNSTVAS LSTSTSTGLS SANSSIASLS TSASSGISTA QSGVNSLSTG LSTTNSTVAS LSTSTSTGLS SATSSIASLS TSTSTGIGSL STGLSTTNSN VASLSSGLSS TNSSLTSLST SASSGISTAQ SGVNSLSTGL STTNSTVASL STSTSTGLSS ATSSIASLST STSTGIGSLS TGLSTTNSNV ASLSSGLSST NSSLTSLSTS ASSGISTAQS GVNSLSTGLS TTNSTVASLS TSTSTGLSSA TSSIASLSTS TSTGIGSLSS GLSTTNSNVA SLSSGLSSTN SSLTSLSTSA SSGISTAQSG VNSLSTGLST ANSTVASLST STSTGLSSAT SSIASLSTST STGIGSLSTG LSTTNSNVAS LSSGLSSTNS SLTSLSTSAS SGISTAQSGV NSLSTGLSTT NSTVASLSTS TSTGLSSATS SIASLSTSTS TGIGSLSTGL STTNSNVASL SSGLSSTNSS LTSLSTSASS GISTAQSGVN SLSTGLSTAN STVASLSTST STGLSSATSS IASLSTSTST GIGSLSTGLS TTNSNVASLS SGLSSTNSSL TSLSTSASSG ISTAQSGVNS LSTGLSTTNS TVASLSTSTS TGLSSATSSI ASLSTSTSTG IGSLSTGLST VATTTNNLGS STAAALGGGA TYNPATGTIS APSYTTYNAN GTTTANTSVG SAIDSINANG IKYFHANSTG PDSVATGADA VAIGTGATAA TANSVALGAN SVTAAAVPTS SATVGSTTLG TFAGSAPVGV VSVGAPSAER QITNVAAGQV TASSTDAING SQLYAVASKL DSTSSSISSL STGLSSATSS ITSLSTSTST GLSSANSSIA SLSTSTSTGI GSLSTGLSTT DSAVASLSTS TSTGLSSATS SITSLSTSTS TGIGSLSTGL STTNSNVASL STSTSTGLSS ATSSIASLST STSTGIGSLS TGLSTTNSTV ASLSTSTSTG LSSANSSIAS LSTSTSTGIG SLSTGLSTTN SNVASLSSGL SSTNSSLTSL STSASSGIST AQSGVNSLST GLSTTNSTVA SLSTSTSTGL SSATSSIASL STSTSTGIGS LSTGLSTTDS TVASLSTSTS TGLSSATSSI ASLSTSTSTG IGSLSTGLST TNSNVASLSS GLSSTNSSLT SLSTSASSGI STAQSGVNSL STGLSTANST VASLSTSTST GLSSANSSIA SLSTSTSTGI GSLSTGLSTT DSTVASLSTS TSTGLSSANS SIASLSTSTS TGIGSLSTGL STTDSTVASL STSTSTGLSS ANSSIASLST STSTGIGSLS TGLSTTNSNV ASLSTSTSTG LSSANSSIAS LSTSTSTGIG SLSTGLSTTN SNVASLSTST STGLNLANSS IASLSTSTST SIDSLSTGLS TTNSNVASLS TSTSTGLSSA NSSIASLSTS TSTGIGSLST GLSTTNSNVA SLSTSTSTGL NLANSSIASL STSTSTGIGS LSTGLSTTNS NVASLSTSTS TGLSSATSSI ASLSTSTSTG IGSLSTGLST TDSTVASLST STSTGLSSAN SSIASLSTST STGIGSLSTG LSTTDSTVAS LSTSTSTGLS SATSSITSLS TSTSTGIGSL STGLSTTNSN VASLSTSTST GLSSATSSIA SLSTSTSTGI GSLSTGLSTT NSTVASLSTS TSTGLSSATS SIASLSTSTS TSIGSLSTGL STTDSTVASL STSTSTGLSS ANSSIASLST STSTGIGSLS TGLSTTDSTV ASLSTSTSTG LSSATSSIAS LSTSTSNGIG SLSTGLSTTN STVASLSTST STGLSSATSS IASLSTSTST GIGSLSTGLS TTNSTVASLS TSTSTGIGSL STGLSTTNST VASLSTSTST GIGSLSTGLS STNSNLVSLS TSASTGIGSL STSIDTTNSN LASLSTSSST GIGSLSTSIN ATNSNLASLS TSASTGIGSL STSISSITTN TTNLGNSTAA ALGGGATYDP ATGAISAPSY TTYNANGTTA TNTSVGAAID NINANGIKYF HANSTDPDSV ATGTNSVAIG PNAVANVDYS VAIGSGATTS AAVPVASASV GGLTFGGFAG SAPIGVFSVG APGAERQITN VAAGRISAAS TDAVNGSQLY ATNSNVASLS TGLNATNSNL ASLSTSTSTA VGSLSTGLST TNSTVASLST STSTSIGSLS TGLSTANSNL ASLSTSTSTG IGSLSTGLAT TNSNVASLST SVTNINTQLT SLSTSITNNV IRSLPASTGV AADMSAPKAT SPSVTAGSNS VALGAGSNDG GRSNVVSVGS DTQQRQITNV AAGTEGTDAV NVNQLNTLST SMSQSLSNQQ TQLNNLGSQL NQTQQQLQQT DTMARQGIAA VAAMASIPHM DRDSNFAMGV GTSSFLGQKA IAVGMQARIT ENLKASLNGG FAGNQKVIGA GMLYQWK
NT Sequence
atgaatcgct cgtaccgctc tatctggaat gaagcacttg gcgcgtgggt cgccgcttcg gaaatctcgt ccgcccgcgg caagccgaac aagtcggccg tcgcgaaggt cgtgaccgcc gccgtgctgg ccgtggtcgt gcaggcagcg catgcaagca cgatcggcgt cggaactggc tcgcttccgg gggggtccgc gtgcggcggc attggcggaa caggaatagg tcctgacggt acgacgaact atacgaaccg ggcgatcacc gacggcagcg gattctactc aaccgttgcc gggtgctcgg ccaacggcag caatgccctg ggcgtgacgc tctatggcgc attcacgacc gtcaccggag acggtggcac ggccatgggc ttcgccgctt acgccgcaaa gtgggcgtcg gccgttggcc ttcaggcgtc cgcgactggc cagtccagct cggcattcgg attcaatgcg ctggcgagcg gcgccaactc gcttgcactc ggctcgtcga gcaccgcggc gtacgcgaac agcgtcgcga tcggccaagg cagcgtcacg ggagcggcgg ccccgacggg gaccggctac ttgacgggca gcgccgcccc gtcttcggag gtgtccgtcg gttcctcgac caatttgcgc cgcatcacca acgttgcgga cggctccgca gcgaatgacg ccgtcacggt tgcccaactg agtacgggca tgagcacgac cactagcgca atcgccagtt tgtcgacctc cacatcgacc gggctttcgt cagccagaag ctcgatcgcg tcgctgtcca cgtcgacttc gaccggcctg agctcggcca ccagctcgat cgcttcactg tcgacttcca cgtcgaccgg catcggttcg ctttccaccg ggctgtcgac caccaacagc aacgtcgcct cgctgtccag cggcctgagc agcacgaaca gctcgctgac ctcgctgtcg acttccgcct cgtcgggcat cagcaccgcg cagagcggtg tcaattcgtt gtccaccggg ctgtcgacca ccaatagcac cgtcgcttcg ctgtccacgt cgacttcgac cggcttgagc tcggcgaata gctcgatcgc ctcgctttcg acttccgctt cgtcgggcat cagcaccgcg cagagcggcg tcaattcgtt gtccaccggg ctgtcgacca ccaatagcac cgtcgcttcg ctgtccacgt cgacctcgac cggcctgagc tcggccacca gctcgatcgc ttcgctgtcg acttccacgt cgaccggtat cggctcgctt tccaccggcc tgtcgaccac caacagcaac gtcgcctcgc tgtccagcgg cctgagcagc acgaatagct cgctgacctc gctgtcgacg tccgcttcgt cgggcatcag caccgcgcag agcggtgtca actcgttgtc caccgggctg tcgaccacca atagcaccgt cgcttcgctg tccacgtcga cttcgaccgg cctgagctcg gccaccagct cgatcgcttc gctgtcgacc tccacgtcga ccggcatcgg ctcgctttcc accggcctgt cgaccaccaa cagcaacgtc gcctcgctgt ccagcggact gagcagcacg aatagctcgc tgacctcgct gtcgacttcc gcctcgtctg gcatcagcac cgcgcagagc ggcgtcaatt cgttgtccac cgggctgtcg accaccaata gcaccgtcgc ttcgctgtcc acgtcgactt cgaccggctt gagctcggcc accagctcga tcgcttcgct gtcgacttcc acttcgaccg gtatcggttc gctttccagc ggcctgtcga ccaccaacag caacgtcgcc tcgctgtcca gcggcctgag cagcacgaat agctcgctga cctcgctgtc gacgtccgct tcgtcgggca tcagcaccgc gcagagcggt gtcaactcgt tgtccaccgg gctgtcgacc gcgaatagca ccgtcgcttc gctgtccacg tcgacttcga ccggcctgag ctcggccacc agctcgatcg cttcgctgtc gacttccacg tcgaccggta tcggctcgct ttccaccggc ctgtcgacca ccaacagcaa cgtcgcctcg ctgtccagcg gactgagcag cacgaatagc tcgctgacct cgctgtcgac ttccgcctcg tctggcatca gcaccgcgca gagcggcgtc aattcgttgt ccaccgggct gtcgaccacc aatagcaccg tcgcttcgct gtccacgtcg acctcgaccg gcctgagctc ggccaccagc tcgatcgctt cgctgtcgac ttccacgtcg accggtatcg gctcgctttc caccggcctg tcgaccacca acagcaacgt cgcctcgctg tccagcggcc tgagcagcac gaatagctcg ctgacctcgc tgtcgacgtc cgcttcgtcg ggcatcagca ccgcgcagag cggtgtcaac tcgttgtcca ccgggctgtc gaccgcgaat agcaccgtcg cttcgctgtc cacgtcgact tcgaccggcc tgagctcggc caccagctcg atcgcttcgc tgtcgacctc cacgtcgacc ggcatcggct cgctttccac cggcctgtcg accaccaaca gcaacgtcgc ctcgctgtcc agcggactga gcagcacgaa tagctcgctg acctcgctgt cgacttccgc ctcgtctggc atcagcaccg cgcagagcgg cgtcaattcg ttgtccaccg ggctgtcgac caccaatagc accgtcgctt cgctgtccac gtcgacttcg accggcttga gctcggccac cagctcgatc gcttcgctgt cgacttccac gtcgaccggc atcggctcgc tttccaccgg cctgtcgacc gtcgcgacga cgaccaacaa tctcggcagc agcaccgccg cggcgctcgg cggcggcgcg acgtacaatc cggccaccgg cacgatttcc gcgccgtcgt acacgacgta caacgcgaac ggcacgacca cggccaacac cagtgtcggc tctgcgatcg acagcatcaa cgcgaacggc atcaagtact tccatgcgaa ctcgactggg ccggacagcg tcgcgaccgg cgcggacgcc gtcgcgatcg gcacgggcgc gacggccgcc acggcgaact cggtcgcgct cggcgcgaac tcggtgacgg ccgcggccgt cccgaccagc agcgcgaccg tcggctcgac cacgctcggt acgttcgcgg gcagcgcgcc cgtgggcgtc gtgagcgtcg gggcgccgag cgcggaacgc cagatcacga acgtcgccgc cggccaggtc accgccagca gcaccgatgc gatcaacggc agccagctct atgcggttgc gtcgaagctc gactcgacct cgagttcgat ttcgtcgctg tcgacgggac tgtcgtcggc caccagttcg attacttcgc tctccacttc cacctcgacg ggcctgagct cggcgaatag ctcgatcgcc tcgctgtcga cctccacgtc gaccggtatc ggttcgctct ccaccggtct gtccacgacc gatagcgccg tcgcctcgct gtccacctcg acttcgaccg gcctgagctc ggccaccagc tcgatcactt cgctgtcgac ttccacgtcg accggcatcg gttcgctttc caccggcctg tccacaacca acagcaacgt cgcttcgctg tccacctcga cttcgaccgg tctgagctcg gccaccagct cgatcgcttc gctgtcgacc tccacgtcga ccggcatcgg ctcgctttcc accggtctgt ccacgaccaa tagcaccgtc gcctcgctgt ccacgtcgac ctcgaccggc ctgagctcgg cgaatagctc gatcgcctcg ctgtcgacct ccacgtcgac cggcatcggc tcgctttcca ccggcctgtc cacgaccaac agcaacgtcg cctcgctgtc cagcggcctg agcagcacga acagctcgct gacctcgctt tcgacttcag cttcgtccgg catcagcacc gcgcagagcg gtgtcaattc gttgtccacc gggctgtcga ccaccaatag caccgtcgct tcgctgtcca cctcgacctc gaccggcttg agctcggcca ccagctcgat cgcttcgctg tcgacttcca cgtcgaccgg catcggctcg ctctccaccg gtctgtccac gaccgatagc accgtcgcgt cgctgtccac gtcgacctcg accggcctga gttcggccac cagctcgatc gcttcgctgt cgacttccac gtcgaccggt atcggctcgc tttccaccgg cctgtcgacc accaacagca acgtcgcctc gctgtccagc ggcctgagca gcacgaatag ctcgctgacc tcgctgtcga cgtccgcttc gtcgggcatc agcaccgcgc agagcggcgt caattcgttg tccaccgggc tgtcgaccgc gaatagcacc gtcgcttcgc tgtccacgtc gacttcgacg ggcctgagct cggcgaacag ctcgatcgct tcgctgtcga cttccacgtc gaccggcatc ggctcgcttt ccaccggcct gtccacgacc gatagcaccg tcgcctcgct gtccacgtcg acttcgaccg gtctgagctc ggcgaatagc tcgatcgcct cgctgtcgac ctccacgtcg accggcatcg gctcgctttc caccggcctg tccacgaccg atagcaccgt cgcctcgctg tccacgtcga cttcgaccgg tctgagctcg gcgaatagct cgatcgcctc gctgtcgacc tccacgtcga ccggtatcgg ctcgctgtcc accggtctgt ccacgaccaa cagcaacgtc gcttcgctgt ccacctcgac ttcgacgggc ctgagctcgg cgaatagctc gatcgcctcg ctgtcgactt ccacgtcgac cggtatcggc tcgctttcca ccggcctgtc cacgaccaac agcaacgtcg cttcgctgtc cacctcgact tcgacgggcc tgaacttggc gaatagctcg atcgcctcgc tgtcgacttc cacgtcgacc agtatcgact cgctttccac cggcctgtcc acgaccaaca gcaacgtcgc ttcgctgtcc acctcgactt cgacgggcct gagctcggcg aatagctcga tcgcctcgct gtcgacttcc acgtcgaccg gtatcggctc gctttccacc ggcctgtcca cgaccaacag caacgtcgct tcgctgtcca cctcgacttc gacgggcctg aacttggcga atagctcgat cgcctcgctg tcgacctcca cgtcgaccgg tatcggctcg ctttccaccg gtctgtccac gaccaacagc aacgtcgctt cgctgtccac ctcgacttcg acgggtctga gctcggccac cagctcgatc gcttcgctgt cgacttccac gtcgaccggc atcggctcgc tctccaccgg tctgtccacg accgatagca ccgtcgcctc gctgtccacg tcgacttcga ccggcctgag ctcggcgaac agctcgatcg cctcgctgtc gacttccacg tcgaccggca tcggctcgct gtccaccggt ctgtccacga ccgatagcac cgtcgcctcg ctgtccacgt cgacttcgac cggcctgagc tcggccacca gctcgatcac ttcgctgtcg acttccacgt cgaccggcat cggttcgctt tccaccggcc tgtccacgac caacagcaac gtcgcttcgc tgtccacctc gacttcgacc ggtctgagct cggccaccag ctcgatcgct tcgctgtcga cctccacgtc gaccggcatc ggctcgcttt ccaccggtct gtcgacgacc aacagcaccg tcgcttcgct gtccacctcg acttcgacgg gcctgagctc ggccaccagc tcgatcgctt cgctgtcgac ttccacgtcg accagtatcg gctcgctttc caccggcctg tccacgaccg atagcaccgt cgcttcgctg tccacgtcga cttcgacggg cctgagctcg gcgaacagct cgatcgcttc gctgtcgact tccacgtcga ccggcatcgg ctcgctgtcc accggtctgt ccacgaccga tagcaccgtt gcttcgctgt ccacgtcgac ttcgaccggt ctgagctcgg ccaccagctc gatcgcttcg ctgtcgacct ccacgtcgaa cggtatcggc tcgctttcca ccggtctgtc cacgaccaac agcaccgtcg cttcgctgtc cacctcgact tcgacgggcc tgagctcggc caccagctcg atcgcctcgc tgtcgacctc cacgtcaacc ggcatcggct cgctttccac cggcctgtcc acgaccaaca gcaccgtcgc ctcgctgtcc acgtcgacct cgaccggcat cggctcgctc tccaccggcc tgtccacgac caacagcacc gtcgcctcgc tgtccacgtc gacttcgacc ggcatcggtt cgctgtccac cggcctgtcg tcgaccaaca gcaacctcgt gtcgctctcc acgtcggcct cgaccggcat cggctcgctg tcgaccagca tcgacacgac caacagcaac ctcgcgtcgc tctccacgtc gagctcgacc ggcatcggct cgctgtcgac cagcatcaac gcgaccaaca gcaacctcgc gtcgctctcc acgtccgcct cgaccggcat cggctcgctg tcgacgagca tcagctcgat cacgacgaac acgacgaacc tcggcaacag caccgccgcg gcgctcggcg gcggcgcgac gtacgatccg gccaccggcg cgatctccgc gccgtcgtac acgacgtaca acgcgaacgg caccaccgcg accaacacca gcgtcggcgc cgcgatcgac aacatcaacg cgaacggcat caagtacttc cacgcgaact cgaccgatcc ggacagcgtc gcgaccggca cgaacagcgt cgcgatcggc ccgaacgcgg tcgcgaacgt cgactactcg gtcgcgatcg gcagcggcgc gacgacctcg gcggccgtgc ccgtcgcgtc ggcgagcgtc ggcggcctca cgttcggcgg cttcgcgggc agcgcgccca tcggcgtgtt cagcgtcggc gcaccgggcg cggaacgcca gatcacgaat gtcgccgccg gccgcatctc cgcggccagc accgacgccg tcaacggcag ccagctctat gcgaccaaca gcaatgtcgc gtcgctgtcg accggtctga acgcgaccaa cagcaacctc gcgtcgctgt ccacgtccac ctcgaccgcc gtcggctcgc tgtccaccgg cctgtccacg accaacagca ccgtcgcctc gctgtccacg tcgacctcga ccagcatcgg ctcgctgtcc accggcctct cgaccgcgaa cagcaacctc gcgtcgctgt ccacgtccac ctcgaccggc atcggctcgc tgtccaccgg cctcgcgacg accaacagca atgtcgcgtc gctgtcgacg agcgtgacca acatcaacac gcagctcacg tcgctgtcga cgtcgatcac gaacaacgtg atccggtcgc tgcccgcgag caccggcgtc gccgcggaca tgagcgcgcc gaaggcgacc tcgccgtccg tcacggccgg ctcgaactcg gtcgcgctcg gcgcgggctc gaacgacggc ggtcgctcga acgtcgtgtc ggtgggcagc gacacgcagc agcgccagat cacgaacgtc gcggccggca ccgagggcac cgacgcggtc aacgtcaacc agttgaatac gctgtcgacg tcgatgtcgc aatcgctgtc gaatcagcaa acgcagctca acaatctcgg ctcgcaactg aaccagacgc agcagcaact gcagcagacc gacacgatgg cccgccaggg gatcgcggcg gtcgcggcga tggcgtcgat tccgcacatg gaccgcgact cgaacttcgc gatgggcgtg ggcacctctt cgttcctcgg ccagaaggcg atcgcggtcg gcatgcaggc gcgcatcacc gagaacctga aggcgtcgct gaacggcggc ttcgccggca atcagaaggt catcggcgcg ggcatgctct atcagtggaa gtaa
Details for BupsA.01663.a.A2.GE26299
HARVESTED ON:
3/10/2009
SEQUENCED ON:
3/26/2009
EXPECTED MW:
19kDa
OBSERVED MW:
19kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BupsA.01663.a.A3.GE26300
HARVESTED ON:
3/10/2009
SEQUENCED ON:
3/26/2009
EXPECTED MW:
18kDa
OBSERVED MW:
19kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with sequence variation
PERCENT IDENTITY:
38
PERCENT COVERAGE:
96
Details for BupsA.01663.a.A5.GE29396
HARVESTED ON:
5/27/2010
SEQUENCED ON:
6/14/2010
EXPECTED MW:
16kDa
OBSERVED MW:
16kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
No Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BupsA.01663.a.A6.GE29397
HARVESTED ON:
5/27/2010
SEQUENCED ON:
6/14/2010
EXPECTED MW:
18kDa
OBSERVED MW:
20kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
96
PERCENT COVERAGE:
88
Details for BupsA.01663.a.A7.GE29398
HARVESTED ON:
5/27/2010
SEQUENCED ON:
6/14/2010
EXPECTED MW:
19kDa
OBSERVED MW:
21kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
No Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
97
PERCENT COVERAGE:
100
Details for BupsA.01663.a.A8.GE29399
HARVESTED ON:
5/27/2010
SEQUENCED ON:
6/14/2010
EXPECTED MW:
42kDa
OBSERVED MW:
43kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
No Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
90
PERCENT COVERAGE:
74
Details for BupsA.01663.a.A9.GE29400
HARVESTED ON:
5/27/2010
SEQUENCED ON:
6/14/2010
EXPECTED MW:
23kDa
OBSERVED MW:
23kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
No Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BupsA.01663.a.A2.PS00262
PURIFICATION DATe:
4/22/2009
CONCENTRATION:
24.89mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
4
VIAL VOLUME:
50µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100