SSGCID: Target Page

BupsA.01455.a

Conserved protein

CENTER ID:	BupsA.01455.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01455.a.A1.GE30941	Full length( BupsA.01455.a )		1	236

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.207
RefSeq:	YP_331628.1
UniProt:	Q3JXS5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAEVVAIRH VHFEDLGSFE QVLGERGRRV RYVDVGGSRV EVLDALEPSL LVVLGGPISA YDDGLYPTTA PLAALVGKRI EAGLPTLGVC LGSQLIARAL GARVYPAAAK ELGWIPLALT DAGRASPLRH VDGAITSMMH WHGDTFDLPA GAIHLASTPA CRNQAFSWGT HVLALQCHPE IRADRFEPWL IGHAGEIAAT PGTDAKRLRE QTAQLGPTLE CAARRMFGEW LDSVGL

NT Sequence

ATGAACGCTG AAGTCGTTGC GATTCGCCAC GTGCATTTCG AGGATCTCGG CAGTTTCGAG CAGGTGCTCG GCGAGCGCGG CCGGCGCGTA CGGTATGTCG ACGTCGGCGG CTCCCGCGTC GAGGTGCTCG ACGCGCTCGA GCCGTCGCTG CTCGTCGTGC TCGGCGGGCC GATCAGCGCT TACGACGACG GGCTGTATCC GACGACGGCG CCGCTCGCGG CGCTCGTCGG CAAGCGCATC GAAGCGGGGC TGCCGACGCT CGGCGTCTGT CTCGGCTCGC AGTTGATCGC GCGTGCGCTC GGCGCGCGCG TGTATCCGGC CGCGGCGAAG GAGCTCGGCT GGATTCCGCT TGCGTTGACG GATGCGGGGC GTGCGTCGCC GCTGCGCCAT GTCGACGGCG CGATCACGTC GATGATGCAT TGGCACGGCG ACACGTTCGA TCTGCCGGCG GGCGCGATTC ATCTCGCGTC GACGCCGGCA TGCCGCAACC AGGCTTTCTC GTGGGGTACG CACGTGCTCG CGCTCCAGTG CCACCCGGAG ATCCGCGCGG ATCGCTTCGA GCCCTGGCTG ATCGGCCATG CGGGCGAGAT CGCGGCCACG CCCGGCACCG ACGCGAAACG ATTGCGCGAA CAGACTGCGC AACTCGGCCC GACGCTCGAA TGCGCGGCGC GCAGGATGTT CGGCGAATGG CTCGATTCCG TCGGGCTG

Details for BupsA.01455.a.A1.GE30941

HARVESTED ON:	11/23/2010
SEQUENCED ON:	12/6/2010
EXPECTED MW:	27kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNAEVVAIR HVHFEDLGSF EQVLGERGRR VRYVDVGGSR VEVLDALEPS LLVVLGGPIS AYDDGLYPTT APLAALVGKR IEAGLPTLGV CLGSQLIARA LGARVYPAAA KELGWIPLAL TDAGRASPLR HVDGAITSMM HWHGDTFDLP AGAIHLASTP ACRNQAFSWG THVLALQCHP EIRADRFEPW LIGHAGEIAA TPGTDAKRLR EQTAQLGPTL ECAARRMFGE WLDSVGL

Validated NT Sequence

ttattacagc ccgacggaat cgagccattc gccgaacatc ctgcgcgccg cgcattcgag cgtcgggccg agttgcgcag tctgttcgcg caatcgtttc gcgtcggtgc cgggcgtggc cgcgatctcg cccgcatggc cgatcagcca gggctcgaag cgatccgcgc ggatctccgg gtggcactgg agcgcgagca cgtgcgtacc ccacgagaaa gcctggttgc ggcatgccgg cgtcgacgcg agatgaatcg cgcccgccgg cagatcgaac gtgtcgccgt gccaatgcat catcgacgtg atcgcgccgt cgacatggcg cagcggcgac gcacgccccg catccgtcaa cgcaagcgga atccagccga gctccttcgc cgcggccgga tacacgcgcg cgccgagcgc acgcgcgatc aactgcgagc cgagacagac gccgagcgtc ggcagccccg cttcgatgcg cttgccgacg agcgccgcga gcggcgccgt cgtcggatac agcccgtcgt cgtaagcgct gatcggcccg ccgagcacga cgagcagcga cggctcgagc gcgtcgagca cctcgacgcg ggagccgccg acgtcgacat accgtacgcg ccggccgcgc tcgccgagca cctgctcgaa actgccgaga tcctcgaaat gcacgtggcg aatcgcaacg acttcagcgt tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgctgaa gtcgttgcga ttcgccacgt gcatttcgag gatctcggca gtttcgagca ggtgctcggc gagcgcggcc ggcgcgtacg gtatgtcgac gtcggcggct cccgcgtcga ggtgctcgac gcgctcgagc cgtcgctgct cgtcgtgctc ggcgggccga tcagcgctta cgacgacggg ctgtatccga cgacggcgcc gctcgcggcg ctcgtcggca agcgcatcga agcggggctg ccgacgctcg gcgtctgtct cggctcgcag ttgatcgcgc gtgcgctcgg cgcgcgcgtg tatccggccg cggcgaagga gctcggctgg attccgcttg cgttgacgga tgcggggcgt gcgtcgccgc tgcgccatgt cgacggcgcg atcacgtcga tgatgcattg gcacggcgac acgttcgatc tgccggcggg cgcgattcat ctcgcgtcga cgccggcatg ccgcaaccag gctttctcgt ggggtacgca cgtgctcgcg ctccagtgcc acccggagat ccgcgcggat cgcttcgagc cctggctgat cggccatgcg ggcgagatcg cggccacgcc cggcaccgac gcgaaacgat tgcgcgaaca gactgcgcaa ctcggcccga cgctcgaatg cgcggcgcgc aggatgttcg gcgaatggct cgattccgtc gggctgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa