BupsA.01370.b

Phosphoglucosamine mutase

CENTER ID: BupsA.01370.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01370.b.A1.GE32553 Full length( BupsA.01370.b ) 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01370.b.A1.PS01151 Full length( BupsA.01370.b ) 1 452

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1668
RefSeq: YP_333021.1
UniProt: Q3JTT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRRYFGTDG IRGKVGDAPI TPDFVLRLGY AAGKVLASAP GRAASGARPT VLIGKDTRVS GYMLEAALEA GFSAAGVDVM LAGPMPTPGV AYLTRALRLS AGVVISASHN PYHDNGIKFF SADGNKLPDE IEAEIEAWLD KPLDCAASDG LGKARRLDDA AGRYIEFCKS TFPAAFDLRG MKLVVDCAHG AAYQVAPHVF HELGADVIPI GVAPNGFNIN DGVGATAPDA LMRAVRANHA DLGIALDGDA DRLLVVDHTG RLYNGDELLY VLVKDRIATN GQVEGAVGTL MTNFAVEVAL KEAGVQFVRA AVGDRYVLEQ LRERGWQLGA EGSGHILSLD RHSTGDGIVS ALLVLAALKR SGKTLAQMLE GVTLFPQKLI NVRMKPGADW KGSEAIRRAI DSAEQALSGS GRVLIRASGT EPVLRVMVEA RQATDANRHA EAIADAVKQA TA
NT Sequence
ATGGGACGTC GTTATTTCGG CACGGACGGC ATTCGCGGCA AGGTCGGCGA CGCGCCGATC ACGCCCGATT TCGTGTTGCG GCTCGGCTAC GCGGCGGGCA AGGTGCTCGC GAGCGCGCCC GGGCGCGCGG CGTCGGGCGC GCGCCCGACG GTGCTGATCG GCAAGGACAC GCGGGTATCG GGCTATATGC TCGAGGCCGC GCTCGAAGCC GGTTTTTCCG CCGCGGGCGT CGACGTGATG CTCGCGGGGC CGATGCCGAC GCCGGGCGTC GCGTATCTGA CGCGCGCGTT GCGGCTGTCG GCGGGCGTCG TGATCAGCGC GTCGCACAAT CCGTATCACG ACAACGGCAT CAAGTTCTTC TCGGCCGACG GCAACAAGCT GCCGGACGAG ATCGAGGCCG AGATCGAGGC GTGGCTCGAC AAGCCGCTCG ACTGCGCGGC ATCGGACGGG CTCGGCAAGG CGCGGCGCCT CGACGACGCG GCCGGCCGCT ACATCGAGTT CTGCAAGAGC ACGTTCCCGG CCGCGTTCGA TCTGCGCGGG ATGAAGCTCG TCGTCGACTG CGCGCACGGC GCCGCGTATC AGGTCGCGCC GCACGTGTTT CACGAGCTCG GTGCGGACGT GATCCCGATC GGCGTCGCGC CGAACGGCTT CAACATCAAC GATGGTGTCG GCGCGACGGC GCCTGATGCG CTGATGCGCG CCGTGCGCGC GAATCATGCC GATCTCGGCA TCGCGCTCGA CGGCGACGCG GACCGCCTGC TCGTCGTCGA CCATACCGGC CGGCTATATA ACGGCGACGA ACTGCTGTAC GTGCTCGTCA AGGATCGCAT TGCAACTAAC GGCCAAGTCG AGGGGGCGGT GGGCACGCTG ATGACGAATT TCGCCGTCGA AGTCGCGCTG AAAGAAGCGG GCGTGCAATT CGTGCGGGCG GCGGTCGGCG ACCGTTACGT GCTCGAGCAG TTGCGCGAGC GTGGCTGGCA GCTCGGCGCG GAAGGTTCGG GCCACATCCT GTCGCTCGAC CGGCATTCGA CGGGCGACGG GATCGTCTCG GCGCTGCTCG TGCTCGCGGC GCTCAAGCGC AGCGGCAAGA CGCTCGCGCA GATGCTCGAA GGCGTCACGC TGTTTCCGCA GAAGCTGATC AACGTCCGGA TGAAGCCGGG CGCCGACTGG AAGGGCAGCG AAGCGATTCG CCGCGCGATC GATTCGGCCG AACAGGCGCT GTCGGGCAGC GGCCGCGTGC TGATTCGCGC GTCGGGGACG GAACCCGTGT TGCGCGTGAT GGTCGAGGCG CGGCAGGCGA CGGACGCGAA CCGTCATGCG GAGGCGATCG CCGACGCGGT CAAGCAGGCC ACCGCT
Details for BupsA.01370.b.A1.GE32553
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 49kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 57
Validated AA Sequence
MKLVVDCAHG AAYQVAPHVF HELGADVIPI GVAPNGFNIN DGVGATAPDA LMRAVRANHA DLGIALDGDA DRLLVVDHTG RLYNGDELLY VLVKDRIATN GQVEGAVGTL MTNFAVEVAL KEAGVQFVRA AVGDRYVLEQ LRERGWQLGA EGSGHILSLD RHSTGDGIVS ALLVLAALKR SGKTLAQMLE GVTLFPQKLI NVRMKPGADW KGSEAIRRAI DSAEQALSGS GRVLIRASGT EPVLRVMVEA RQATDANRHA EAIADAVKQA TA
Validated NT Sequence
ttattaagcg gtggcctgct tgaccgcgtc ggcgatcgcc tccgcatgac ggttcgcgtc cgtcgcctgc cgcgcctcga ccatcacgcg caacacgggt tccgtccccg acgcgcgaat cagcacgcgg ccgctgcccg acagcgcctg ttcggccgaa tcgatcgcgc ggcgaatcgc ttcgctgccc ttccagtcgg cgcccggctt catccggacg ttgatcagct tctgcggaaa cagcgtgacg ccttcgagca tctgcgcgag cgtcttgccg ctgcgcttga gcgccgcgag cacgagcagc gccgagacga tcccgtcgcc cgtcgaatgc cggtcgagcg acaggatgtg gcccgaacct tccgcgccga gctgccagcc acgctcgcgc aactgctcga gcacgtaacg gtcgccgacc gccgcccgca cgaattgcac gcccgcttct ttcagcgcga cttcgacggc gaaattcgtc atcagcgtgc ccaccgcccc ctcgacttgg ccgttagttg caatgcgatc cttgacgagc acgtacagca gttcgtcgcc gttatatagc cggccggtat ggtcgacgac gagcaggcgg tccgcgtcgc cgtcgagcgc gatgccgaga tcggcatgat tcgcgcgcac ggcgcgcatc agcgcatcag gcgccgtcgc gccgacacca tcgttgatgt tgaagccgtt cggcgcgacg ccgatcggga tcacgtccgc accgagctcg tgaaacacgt gcggcgcgac ctgatacgcg gcgccgtgcg cgcagtcgac gacgagcttc atcccgcgca gatcgaacgc ggccgggaac gtgctcttgc agaactcgat gtagcggccg ggncgcgtcg tcgaggcgcc gcgccttgcc gagcccgtcc gatgccgcgc agtcgagcgg cttgtcgagc cacgcctcga tctcggcctc gatctcgtcc ggcagcttgt tgccgtcggc cgagaagaac ttgatgccgt tgtcgtgata cggattgtgc gacgcgctga tcacgacgcc cgccgacagc cgcaacgcgc gcgtcagata cgcgacgccc ggcgtcggca tcggccccgc gagcatcacg tcgacgcccg cggcggaaaa accggcttcg agcgcggcct cgagcatata gcccgatacc cgcgtgtcct tgccgatcag caccgtcggg cgcgcgcccg acgccgcgcg cccgggcgcg ctcgcgagca ccttgcccgc cgcgtagccg agccgcaaca cgaaatcggg cgtgatcggc gcgtcgccga ccttgccgcg aatgccgtcc gtgccgaaat aacgacgtcc catcgaacca ggaccctggg tctgagcttc cagggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRRYFGTD GIRGKVGDAP ITPDFVLRLG YAAGKVLASA PGRAASGARP TVLIGKDTRV SGYMLEAALE AGFSAAGVDV MLAGPMPTPG VAYLTRALRL SAGVVISASH NPYHDNGIKF FSADGNKLPD EIEAEIEAWL DKPLDCAASD GLGKARRLDD AAGRYIEFCK STFPAAFDLR GMKLVVDCAH GAAYQVAPHV FHELGADVIP IGVAPNGFNI NDGVGATAPD ALMRAVRANH ADLGIALDGD ADRLLVVDHT GRLYNGDELL YVLVKDRIAT NGQVEGAVGT LMTNFAVEVA LKEAGVQFVR AAVGDRYVLE QLRERGWQLG AEGSGHILSL DRHSTGDGIV SALLVLAALK RSGKTLAQML EGVTLFPQKL INVRMKPGAD WKGSEAIRRA IDSAEQALSG SGRVLIRASG TEPVLRVMVE ARQATDANRH AEAIADAVKQ ATA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggacgtcgt tatttcggca cggacggcat tcgcggcaag gtcggcgacg cgccgatcac gcccgatttc gtgttgcggc tcggctacgc ggcgggcaag gtgctcgcga gcgcgcccgg gcgcgcggcg tcgggcgcgc gcccgacggt gctgatcggc aaggacacgc gggtatcggg ctatatgctc gaggccgcgc tcgaagccgg tttttccgcc gcgggcgtcg acgtgatgct cgcggggccg atgccgacgc cgggcgtcgc gtatctgacg cgcgcgttgc ggctgtcggc gggcgtcgtg atcagcgcgt cgcacaatcc gtatcacgac aacggcatca agttcttctc ggccgacggc aacaagctgc cggacgagat cgaggccgag atcgaggcgt ggctcgacaa gccgctcgac tgcgcggcat cggacgggct cggcaaggcg cggcgcctcg acgacgcggc cggccgctac atcgagttct gcaagagcac gttcccggcc gcgttcgatc tgcgcgggat gaagctcgtc gtcgactgcg cgcacggcgc cgcgtatcag gtcgcgccgc acgtgtttca cgagctcggt gcggacgtga tcccgatcgg cgtcgcgccg aacggcttca acatcaacga tggtgtcggc gcgacggcgc ctgatgcgct gatgcgcgcc gtgcgcgcga atcatgccga tctcggcatc gcgctcgacg gcgacgcgga ccgcctgctc gtcgtcgacc ataccggccg gctatataac ggcgacgaac tgctgtacgt gctcgtcaag gatcgcattg caactaacgg ccaagtcgag ggggcggtgg gcacgctgat gacgaatttc gccgtcgaag tcgcgctgaa agaagcgggc gtgcaattcg tgcgggcggc ggtcggcgac cgttacgtgc tcgagcagtt gcgcgagcgt ggctggcagc tcggcgcgga aggttcgggc cacatcctgt cgctcgaccg gcattcgacg ggcgacggga tcgtctcggc gctgctcgtg ctcgcggcgc tcaagcgcag cggcaagacg ctcgcgcaga tgctcgaagg cgtcacgctg tttccgcaga agctgatcaa cgtccggatg aagccgggcg ccgactggaa gggcagcgaa gcgattcgcc gcgcgatcga ttcggccgaa caggcgctgt cgggcagcgg ccgcgtgctg attcgcgcgt cggggacgga acccgtgttg cgcgtgatgg tcgaggcgcg gcaggcgacg gacgcgaacc gtcatgcgga ggcgatcgcc gacgcggtca agcaggccac cgcttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.01370.b.A1.PS01151
PURIFICATION DATe: 7/12/2011
CONCENTRATION: 51.35mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 57
Protocol Notes
notes unavailable
Validated AA Sequence
MKLVVDCAHG AAYQVAPHVF HELGADVIPI GVAPNGFNIN DGVGATAPDA LMRAVRANHA DLGIALDGDA DRLLVVDHTG RLYNGDELLY VLVKDRIATN GQVEGAVGTL MTNFAVEVAL KEAGVQFVRA AVGDRYVLEQ LRERGWQLGA EGSGHILSLD RHSTGDGIVS ALLVLAALKR SGKTLAQMLE GVTLFPQKLI NVRMKPGADW KGSEAIRRAI DSAEQALSGS GRVLIRASGT EPVLRVMVEA RQATDANRHA EAIADAVKQA TA
Validated NT Sequence
ttattaagcg gtggcctgct tgaccgcgtc ggcgatcgcc tccgcatgac ggttcgcgtc cgtcgcctgc cgcgcctcga ccatcacgcg caacacgggt tccgtccccg acgcgcgaat cagcacgcgg ccgctgcccg acagcgcctg ttcggccgaa tcgatcgcgc ggcgaatcgc ttcgctgccc ttccagtcgg cgcccggctt catccggacg ttgatcagct tctgcggaaa cagcgtgacg ccttcgagca tctgcgcgag cgtcttgccg ctgcgcttga gcgccgcgag cacgagcagc gccgagacga tcccgtcgcc cgtcgaatgc cggtcgagcg acaggatgtg gcccgaacct tccgcgccga gctgccagcc acgctcgcgc aactgctcga gcacgtaacg gtcgccgacc gccgcccgca cgaattgcac gcccgcttct ttcagcgcga cttcgacggc gaaattcgtc atcagcgtgc ccaccgcccc ctcgacttgg ccgttagttg caatgcgatc cttgacgagc acgtacagca gttcgtcgcc gttatatagc cggccggtat ggtcgacgac gagcaggcgg tccgcgtcgc cgtcgagcgc gatgccgaga tcggcatgat tcgcgcgcac ggcgcgcatc agcgcatcag gcgccgtcgc gccgacacca tcgttgatgt tgaagccgtt cggcgcgacg ccgatcggga tcacgtccgc accgagctcg tgaaacacgt gcggcgcgac ctgatacgcg gcgccgtgcg cgcagtcgac gacgagcttc atcccgcgca gatcgaacgc ggccgggaac gtgctcttgc agaactcgat gtagcggccg ggncgcgtcg tcgaggcgcc gcgccttgcc gagcccgtcc gatgccgcgc agtcgagcgg cttgtcgagc cacgcctcga tctcggcctc gatctcgtcc ggcagcttgt tgccgtcggc cgagaagaac ttgatgccgt tgtcgtgata cggattgtgc gacgcgctga tcacgacgcc cgccgacagc cgcaacgcgc gcgtcagata cgcgacgccc ggcgtcggca tcggccccgc gagcatcacg tcgacgcccg cggcggaaaa accggcttcg agcgcggcct cgagcatata gcccgatacc cgcgtgtcct tgccgatcag caccgtcggg cgcgcgcccg acgccgcgcg cccgggcgcg ctcgcgagca ccttgcccgc cgcgtagccg agccgcaaca cgaaatcggg cgtgatcggc gcgtcgccga ccttgccgcg aatgccgtcc gtgccgaaat aacgacgtcc catcgaacca ggaccctggg tctgagcttc cagggtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRRYFGTD GIRGKVGDAP ITPDFVLRLG YAAGKVLASA PGRAASGARP TVLIGKDTRV SGYMLEAALE AGFSAAGVDV MLAGPMPTPG VAYLTRALRL SAGVVISASH NPYHDNGIKF FSADGNKLPD EIEAEIEAWL DKPLDCAASD GLGKARRLDD AAGRYIEFCK STFPAAFDLR GMKLVVDCAH GAAYQVAPHV FHELGADVIP IGVAPNGFNI NDGVGATAPD ALMRAVRANH ADLGIALDGD ADRLLVVDHT GRLYNGDELL YVLVKDRIAT NGQVEGAVGT LMTNFAVEVA LKEAGVQFVR AAVGDRYVLE QLRERGWQLG AEGSGHILSL DRHSTGDGIV SALLVLAALK RSGKTLAQML EGVTLFPQKL INVRMKPGAD WKGSEAIRRA IDSAEQALSG SGRVLIRASG TEPVLRVMVE ARQATDANRH AEAIADAVKQ ATA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGACGTCGT TATTTCGGCA CGGACGGCAT TCGCGGCAAG GTCGGCGACG CGCCGATCAC GCCCGATTTC GTGTTGCGGC TCGGCTACGC GGCGGGCAAG GTGCTCGCGA GCGCGCCCGG GCGCGCGGCG TCGGGCGCGC GCCCGACGGT GCTGATCGGC AAGGACACGC GGGTATCGGG CTATATGCTC GAGGCCGCGC TCGAAGCCGG TTTTTCCGCC GCGGGCGTCG ACGTGATGCT CGCGGGGCCG ATGCCGACGC CGGGCGTCGC GTATCTGACG CGCGCGTTGC GGCTGTCGGC GGGCGTCGTG ATCAGCGCGT CGCACAATCC GTATCACGAC AACGGCATCA AGTTCTTCTC GGCCGACGGC AACAAGCTGC CGGACGAGAT CGAGGCCGAG ATCGAGGCGT GGCTCGACAA GCCGCTCGAC TGCGCGGCAT CGGACGGGCT CGGCAAGGCG CGGCGCCTCG ACGACGCGGC CGGCCGCTAC ATCGAGTTCT GCAAGAGCAC GTTCCCGGCC GCGTTCGATC TGCGCGGGAT GAAGCTCGTC GTCGACTGCG CGCACGGCGC CGCGTATCAG GTCGCGCCGC ACGTGTTTCA CGAGCTCGGT GCGGACGTGA TCCCGATCGG CGTCGCGCCG AACGGCTTCA ACATCAACGA TGGTGTCGGC GCGACGGCGC CTGATGCGCT GATGCGCGCC GTGCGCGCGA ATCATGCCGA TCTCGGCATC GCGCTCGACG GCGACGCGGA CCGCCTGCTC GTCGTCGACC ATACCGGCCG GCTATATAAC GGCGACGAAC TGCTGTACGT GCTCGTCAAG GATCGCATTG CAACTAACGG CCAAGTCGAG GGGGCGGTGG GCACGCTGAT GACGAATTTC GCCGTCGAAG TCGCGCTGAA AGAAGCGGGC GTGCAATTCG TGCGGGCGGC GGTCGGCGAC CGTTACGTGC TCGAGCAGTT GCGCGAGCGT GGCTGGCAGC TCGGCGCGGA AGGTTCGGGC CACATCCTGT CGCTCGACCG GCATTCGACG GGCGACGGGA TCGTCTCGGC GCTGCTCGTG CTCGCGGCGC TCAAGCGCAG CGGCAAGACG CTCGCGCAGA TGCTCGAAGG CGTCACGCTG TTTCCGCAGA AGCTGATCAA CGTCCGGATG AAGCCGGGCG CCGACTGGAA GGGCAGCGAA GCGATTCGCC GCGCGATCGA TTCGGCCGAA CAGGCGCTGT CGGGCAGCGG CCGCGTGCTG ATTCGCGCGT CGGGGACGGA ACCCGTGTTG CGCGTGATGG TCGAGGCGCG GCAGGCGACG GACGCGAACC GTCATGCGGA GGCGATCGCC GACGCGGTCA AGCAGGCCAC CGCTTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA