SSGCID: Target Page

BupsA.01365.b

Oxidoreductase, short-chain dehydrogenase/reductase family

CENTER ID:	BupsA.01365.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01365.b.A1.GE30937	Full length( BupsA.01365.b )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.5492
RefSeq:	YP_336547.1
UniProt:	Q3JIQ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQLSSDPIS GASLAGRTAF VTGGGRGLGA AICGELARAG AHVVVADLDE ARARGVAERL ARDGARAVPL GVDVGDETSV RHAFDAARES LGDIDVIVNN AAIDVTAAID DVSVDAWRQV LTTNLFGPYL LCHAAVPMMK ARGRGHIVNI ASTAAKRAWP NASAYHATKW GLLGLSHALH AELRPSGVHV SAIVAGGMRT PFLLDRFPDI DADTLQPPEH VAAAVRFVLT QPPGTVIPEV MVLPVKETSW P

NT Sequence

GTGTCCCAGC TATCCTCCGA TCCAATCTCC GGCGCGTCGC TCGCCGGCCG GACCGCGTTC GTCACGGGCG GCGGGCGCGG CCTCGGTGCC GCGATCTGCG GCGAGCTCGC GCGCGCCGGC GCGCACGTCG TCGTCGCCGA TCTCGACGAA GCGCGCGCGC GCGGCGTCGC CGAGCGGCTT GCGCGGGACG GCGCGCGCGC GGTGCCGCTC GGCGTCGACG TCGGCGACGA GACGTCGGTG CGCCACGCAT TCGACGCGGC GCGCGAATCG CTCGGCGACA TCGACGTGAT CGTCAACAAC GCGGCGATCG ACGTGACGGC CGCGATCGAC GACGTGAGCG TCGACGCCTG GCGGCAGGTG CTGACGACGA ACCTGTTCGG TCCGTACCTG CTGTGTCACG CCGCCGTGCC GATGATGAAG GCGCGCGGCC GCGGGCACAT CGTCAACATC GCGTCGACCG CGGCCAAGCG CGCGTGGCCG AATGCGTCCG CGTATCACGC GACGAAGTGG GGGCTGCTCG GCCTGTCGCA TGCATTGCAC GCGGAGCTGC GCCCGTCGGG CGTGCATGTG TCGGCGATCG TCGCGGGCGG CATGCGCACG CCGTTCCTGC TCGATCGCTT TCCCGACATC GACGCCGATA CGTTGCAGCC GCCCGAGCAC GTCGCGGCGG CCGTGCGCTT CGTGCTCACG CAGCCGCCGG GCACGGTGAT TCCCGAGGTG ATGGTGCTGC CCGTGAAGGA AACGTCATGG CCG

Details for BupsA.01365.b.A1.GE30937

HARVESTED ON:	11/23/2010
SEQUENCED ON:	12/6/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	39
PERCENT COVERAGE:	86

Validated AA Sequence

MAHHHHHHMG TLEXQTQGPX SMVSQLSSDP XSGASLAGRT AFVTGGGRGL GAARRRRRRR DVGAPRIRRG ARIARRHRRD RQQRGDRRDG RDRRRERRRL AAGADDEPVR SVPAVSRRRA DDEGARPRAH RQHRVDRGQA RVAECVRVSR DEVGAARPVA CIARGAAPVG RACVGDRRGR HAHAVPARSL SRHRRRYVAA ARARRGGRAL RAHAAAGHGD SRGDGAAREG NVMAV

Validated NT Sequence

attacggcca tgacgtttcc ttcacgggca gcaccatcac ctcgggaatc accgtgcccg gcggctgcgt gagcacgaag cgcacggccg ccgcgacgtg ctcgggcggc tgcaacgtat cggcgtcgat gtcgggaaag cgatcgagca ggaacggcgt gcgcatgccg cccgcgacga tcgccgacac atgcacgccc gacgggcgca gctccgcgtg caatgcatgc gacaggccga gcagccccca cttcgtcgcg tgatacgcgg acgcattcgg ccacgcgcgc ttggccgcgg tcgacgcgat gttgacgatg tgcccgcggc cgcgcgcctt catcatcggc acggcggcgt gacacagcag gtacggaccg aacaggttcg tcgtcagcac ctgccgccag gcgtcgacgc tcacgtcgtc gatcgcggcc gtcacgtcga tcgccgcgtt gttgacgatc acgtcgatgt cgccgagcga ttcgcgcgcc gcgtcgaatg cgtggcgcac cgacgtctcg tcgccgacgt cgacgccgag cggcaccgag gccgcgcccg ccgcccgtga cgaacgcggt ccggccggcg agcgacgcgc cgganattgg atcggaggat agctgggaca ccatcgaanc aggaccctgg gtctganctt ccagggtacc catatgatgg tgatggtgat gagccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSQLSSDP ISGASLAGRT AFVTGGGRGL GAAICGELAR AGAHVVVADL DEARARGVAE RLARDGARAV PLGVDVGDET SVRHAFDAAR ESLGDIDVIV NNAAIDVTAA IDDVSVDAWR QVLTTNLFGP YLLCHAAVPM MKARGRGHIV NIASTAAKRA WPNASAYHAT KWGLLGLSHA LHAELRPSGV HVSAIVAGGM RTPFLLDRFP DIDADTLQPP EHVAAAVRFV LTQPPGTVIP EVMVLPVKET SWP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcccag ctatcctccg atccaatctc cggcgcgtcg ctcgccggcc ggaccgcgtt cgtcacgggc ggcgggcgcg gcctcggtgc cgcgatctgc ggcgagctcg cgcgcgccgg cgcgcacgtc gtcgtcgccg atctcgacga agcgcgcgcg cgcggcgtcg ccgagcggct tgcgcgggac ggcgcgcgcg cggtgccgct cggcgtcgac gtcggcgacg agacgtcggt gcgccacgca ttcgacgcgg cgcgcgaatc gctcggcgac atcgacgtga tcgtcaacaa cgcggcgatc gacgtgacgg ccgcgatcga cgacgtgagc gtcgacgcct ggcggcaggt gctgacgacg aacctgttcg gtccgtacct gctgtgtcac gccgccgtgc cgatgatgaa ggcgcgcggc cgcgggcaca tcgtcaacat cgcgtcgacc gcggccaagc gcgcgtggcc gaatgcgtcc gcgtatcacg cgacgaagtg ggggctgctc ggcctgtcgc atgcattgca cgcggagctg cgcccgtcgg gcgtgcatgt gtcggcgatc gtcgcgggcg gcatgcgcac gccgttcctg ctcgatcgct ttcccgacat cgacgccgat acgttgcagc cgcccgagca cgtcgcggcg gccgtgcgct tcgtgctcac gcagccgccg ggcacggtga ttcccgaggt gatggtgctg cccgtgaagg aaacgtcatg gccgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa