BupsA.01181.a

Thymidylate synthase (EC 2.1.1.45)

CENTER ID: BupsA.01181.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01181.a.D12.GE00001 BupsA.01181.a 1 284
BupsA.01181.a.D14.GE00001 BupsA.01181.a 4 323
BupsA.01181.a.D16.GE00001 BupsA.01181.a 4 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01181.a.D14.PD00061 BupsA.01181.a 4 323
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127385
PATRIC ID: fig|320372.6.peg.3075
RefSeq: YP_334325.1
UniProt: Q3JQ28
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKQYLDLVRT ILDTGTWQSN RTGIRTIGIP GAMLRFDLQQ GFPAVTTKKL AFKSAIGELV GFLRATRSAA DFRALGCKVW DANANENAQW LANPYRRGVD DLGDVYGVQW RRWPGYKVLD AHADAQIADA TSRGFRAVAR FDEDGAQKVL LHKAIDQLRD CLDTIVRDPS SRRILFHGWN PAVLDEIALP ACHLLYQFLP NVERREISLC LYIRSNDVGL GTPFNLAEGA ALLTLVGRLT GYTPRWFTYF IGDAHIYENQ LGMLKQQLER EPFDSPRLEI AERVPEYAKT GQYEPQWLER IEPSDFTLVG YRHHEPLSAP MAV
NT Sequence
atgaaacagt atctcgatct cgttcgcacc attctcgata ccggcacctg gcagagcaac cgcacgggca ttcgcacgat cggcatcccc ggcgcgatgc tgcgcttcga tctgcagcag ggctttcccg cagtgacgac gaaaaagctc gcgttcaagt ccgcgatcgg cgagctcgtc ggctttctgc gcgcgacgcg cagcgcggcc gatttccgcg cgctcggctg caaggtatgg gacgcgaatg cgaatgagaa cgcgcagtgg ctcgcgaatc cgtatcgccg cggcgtcgac gacctcggcg acgtgtacgg cgtccaatgg cggcgctggc cgggctacaa ggtgctcgac gcgcatgccg acgcgcagat cgccgatgcg acgtcgcgcg gttttcgcgc cgtcgcgcgc ttcgacgagg acggcgcgca gaaggtgctg ctgcacaagg cgatcgacca gttgcgagac tgcctcgaca cgatcgtgcg cgacccgtcg agccggcgca tcctgttcca cggctggaat cccgcggtgc tcgacgagat cgcgctgccc gcgtgccatc tgctgtatca attcctgccg aatgtcgagc gtcgcgaaat ctcgctgtgc ctgtacatcc gcagcaacga cgtcgggctc ggcacgccgt tcaatctcgc cgaaggcgcg gcgctcctca cgctcgttgg ccggctgacg ggctatacgc cgcgctggtt cacgtacttc atcggcgatg cgcacatcta cgagaatcag ctcggcatgc tcaagcagca gctcgaacgc gagccgttcg acagcccgcg gctcgagatc gccgagcgcg tgcccgagta cgcgaaaacg gggcagtacg agccacaatg gcttgaacgc atcgagccgt cggatttcac gctcgtcggc tatcgtcacc acgaaccgct gagcgcgccg atggccgttt ga
Details for BupsA.01181.a.D12.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/28/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 14
PERCENT COVERAGE: 31
Validated AA Sequence
MPIVRIPVXL DCQVPVSRMV RTKSRYCFIP XICSXCASIM SLSXMXRSXX XWSXXXXIXS YXKXXXPFXX PXXXXXXXXX XXXGXXXXLX XXXXLXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagctttta gacgcgctca gctatttcca ggcgtggaga gtcgaatggt tcacgttcga gctgctgctt cagcataccc agctgatttt catagatgtg ggcatcgcct atgaaataag taaaccagcg cggagtgtag ccagtaagac ggcctaccag ggtcaggagt gcagcacctt cagccaggtt gaacggagtg cctaaaccca cgtcgttaga acggatatac agacacaggc taatttcacg acgttcaacg ttcggcagga actggtacag caggtggcac gccggaagag ctatttcgtc aagaacagca gggttccaac cgtgaaacag gatacgacgg ctcgacgggt cacgaactat agtgtccagg caatcacgca gttggtcgat cgctttgtgg agcagaactt tctgggcacc atcttcatca aagcgagcca cagcgcggaa accgcggcta gtagcatcag ctatctgagc gtcggcgtgg gcgtcgagga ccttgtaacc tggccaacgg cgccattgca caccatacac gtcacccaga tcatcaacgc cacggcggta tgggttagct aaccactgag cattctcgtt agcgttagcg tcccagactt tacagcccan ggcacggaag tcagcagcgg aacgggtagc acgcagaaac caactaattc gccgattgcg gatttgaatg cgagcttctt cgtagtcacg gccgggaaac cctgttggag gtcaaaacgg ancatagcgc ctgggatgcc tatagtacgg ataccggtac ngttagactg ccaagtaccg gtgtccagga tggtacgaac taagtcnagg tactgcttca ttccancgat ttgttcacng tgtgcttcga taatgtcgtt atcttnnatg tnnagatctt ncnnantctg gtctgnctgn anangnatan nntcgtacan aaagnncnnn nntccatttn nnnnnccnng nnnttnnnna ntgcntnntc ngnnnannnn nnngggnnnn nnnnntnntc ttgannaann ntnnnnnctn cnnnncnnnn nncnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngn nnnnccnnnn nnnnnncc
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMKQY LDLVRTILDT GTWQSNRTGI RTIGIPGAML RFDLQQGFPA VTTKKLAFKS AIGELVGFLR ATRSAADFRA LGCKVWDANA NENAQWLANP YRRGVDDLGD VYGVQWRRWP GYKVLDAHAD AQIADATSRG FRAVARFDED GAQKVLLHKA IDQLRDCLDT IVRDPSSRRI LFHGWNPAVL DEIALPACHL LYQFLPNVER REISLCLYIR SNDVGLGTPF NLAEGAALLT LVGRLTGYTP RWFTYFIGDA HIYENQLGML KQQLEREPFD SPRLEIAERV
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaagcagtac cttgacttag ttcgtaccat cctggacacc ggtacttggc agtctaaccg taccggtatc cgtactatag gcatcccagg cgctatgctc cgttttgacc tccaacaggg tttcccggcc gtgactacga agaagctcgc attcaaatcc gcaatcggcg aattagttgg tttcctgcgt gctacccgtt ccgctgctga cttccgtgcc ctgggctgta aagtctggga cgctaacgct aacgagaatg ctcagtggtt agctaaccca taccgccgtg gcgttgatga tctgggtgac gtgtatggtg tgcaatggcg ccgttggcca ggttacaagg tcctcgacgc ccacgccgac gctcagatag ctgatgctac tagccgcggt ttccgcgctg tggctcgctt tgatgaagat ggtgcccaga aagttctgct ccacaaagcg atcgaccaac tgcgtgattg cctggacact atagttcgtg acccgtcgag ccgtcgtatc ctgtttcacg gttggaaccc tgctgttctt gacgaaatag ctcttccggc gtgccacctg ctgtaccagt tcctgccgaa cgttgaacgt cgtgaaatta gcctgtgtct gtatatccgt tctaacgacg tgggtttagg cactccgttc aacctggctg aaggtgctgc actcctgacc ctggtaggcc gtcttactgg ctacactccg cgctggttta cttatttcat aggcgatgcc cacatctatg aaaatcagct gggtatgctg aagcagcagc tcgaacgtga accattcgac tctccacgcc tggaaatagc tgagcgcgtc taaaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for BupsA.01181.a.D14.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/29/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 95
PERCENT COVERAGE: 69
Validated AA Sequence
MXRFDLXQXX PXVXTKKLXF KSXXGELVGF XRATRSAADF RAXGCKVWDA NANENAQWLA NPYRRGVDDL GDVYGVQWRR WPGYKVLDAH ADAQIXDATS RGFRAVARFD EDGAQKVLLH KAIDQLRDCL DTIVRDPSSR RILFHGWNPA VLDEIALPAC HLLYQFLPNV ERREISLCLY IRSNDVGLGT PFNLAEGAAL LTLVGRLTGY TPRWFTYFIG DAHIYENQLG MLKQQLEREP FDSPRLEIAE RVPEYAKTGQ YEPQWLERIE PSDFTLVGYR HHEPLSAPMA V
Validated NT Sequence
gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc ttttacacag ccataggagc gctcagcggt tcatggtgac ggtaaccaac cagggtgaaa tcggacggct cgatgcgttc gagccactgt ggttcgtact gaccagtttt agcgtattct gggacgcgct cagctatttc caggcgtgga gagtcgaatg gttcacgttc gagctgctgc ttcagcatac ccagctgatt ttcatagatg tgggcatcgc ctatgaaata agtaaaccag cgcggagtgt agccagtaag acggcctacc agggtcagga gtgcagcacc ttcagccagg ttgaacggag tgcctaaacc cacgtcgtta gaacggatat acagacacag gctaatttca cgacgttcaa cgttcggcag gaactggtac agcaggtggc acgccggaag agctatttcg tcaagaacag cagggttcca accgtgaaac aggatacgac ggctcgacgg gtcacgaact atagtgtcca ggcaatcacg cagttggtcg atcgctttgt ggagcagaac tttctgggca ccatcttcat caaagcgagc cacagcgcgg aaaccgcggc tagtagcatc anctatctga gcgtcngcgt gggcgtcgag gaccttgtaa cctggccaac ggcgccattg cacaccatac acgtcaccca gatcatcnac gccacggcgg tatgggttag ctaaccactg agcattctcg ttagcgttag cgtcccagac tttacagccc nnggcacgga agtcagcagc ggaacgggta gcacgcnnga aaccaactaa ttcgccgann gnggatttga atgnnagctt cttcgtnntc acggncggnn aancctgntg gaggtcaaaa cggancatan cgcctgggnn gcntatanna cgnataccng nanggnnann ctnnnnngta nnngnntccn nnnnggnncg nactnagtcn nggnatcnat nnnnnanggn nnnntnnnna nnncntntnt nnnnnncnnn tnnnnnnnnc nnanncnnnn ncnnnnnnnn nannnnnnnn nntnnnnnnn nngnnnnnnn gnnnnnnnnn nnnnnctnnn nnnnnaanan tnnnnnnnnc nnnnnnnnaa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYLDL VRTILDTGTW QSNRTGIRTI GIPGAMLRFD LQQGFPAVTT KKLAFKSAIG ELVGFLRATR SAADFRALGC KVWDANANEN AQWLANPYRR GVDDLGDVYG VQWRRWPGYK VLDAHADAQI ADATSRGFRA VARFDEDGAQ KVLLHKAIDQ LRDCLDTIVR DPSSRRILFH GWNPAVLDEI ALPACHLLYQ FLPNVERREI SLCLYIRSND VGLGTPFNLA EGAALLTLVG RLTGYTPRWF TYFIGDAHIY ENQLGMLKQQ LEREPFDSPR LEIAERVPEY AKTGQYEPQW LERIEPSDFT LVGYRHHEPL SAPMAV
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata ccttgactta gttcgtacca tcctggacac cggtacttgg cagtctaacc gtaccggtat ccgtactata ggcatcccag gcgctatgct ccgttttgac ctccaacagg gtttcccggc cgtgactacg aagaagctcg cattcaaatc cgcaatcggc gaattagttg gtttcctgcg tgctacccgt tccgctgctg acttccgtgc cctgggctgt aaagtctggg acgctaacgc taacgagaat gctcagtggt tagctaaccc ataccgccgt ggcgttgatg atctgggtga cgtgtatggt gtgcaatggc gccgttggcc aggttacaag gtcctcgacg cccacgccga cgctcagata gctgatgcta ctagccgcgg tttccgcgct gtggctcgct ttgatgaaga tggtgcccag aaagttctgc tccacaaagc gatcgaccaa ctgcgtgatt gcctggacac tatagttcgt gacccgtcga gccgtcgtat cctgtttcac ggttggaacc ctgctgttct tgacgaaata gctcttccgg cgtgccacct gctgtaccag ttcctgccga acgttgaacg tcgtgaaatt agcctgtgtc tgtatatccg ttctaacgac gtgggtttag gcactccgtt caacctggct gaaggtgctg cactcctgac cctggtaggc cgtcttactg gctacactcc gcgctggttt acttatttca taggcgatgc ccacatctat gaaaatcagc tgggtatgct gaagcagcag ctcgaacgtg aaccattcga ctctccacgc ctggaaatag ctgagcgcgt cccagaatac gctaaaactg gtcagtacga accacagtgg ctcgaacgca tcgagccgtc cgatttcacc ctggttggtt accgtcacca tgaaccgctg agcgctccta tggctgtgta aaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for BupsA.01181.a.D16.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/29/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 88
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG YLDLVRTILD TGTWQSNRTG IRTIGIPGAM LRFDLQQGFP AVTTKKLAFK SAIGELVGFL RATRSAADFR ALGCKVWDAN ANENAQWLAN PYRRGVDDLG DVYGVQWRRW PGYKVLDAHA DAQIADATSR GFRAVARFDE DGAQKVLLHK AIDQLRDCLD TIVRDPSSRR ILFHGWNPAV LDEIALPACH LLYQFLPNVE RREISLCLYI RSNDVGLGTP FNLAEGAALL TLVGRLTGYT PRWFTYFIGD AHIYENQLGM LKQQLEREPF DSPRLEIAER VPEYAKTGQY EPQWLERIEP SDFTLVGYRH XE
Validated NT Sequence
tnattcntgg tgacggtaac caaccagggt gaaatcggac ggctcgatgc gttcgagcca ctgtggttcg tactgaccag ttttagcgta ttctgggacg cgctcagcta tttccaggcg tggagagtcg aatggttcac gttcgagctg ctgcttcagc atacccagct gattttcata gatgtgggca tcgcctatga aataagtaaa ccagcgcgga gtgtagccag taagacggcc taccagggtc aggagtgcag caccttcagc caggttgaac ggagtgccta aacccacgtc gttagaacgg atatacagac acaggctaat ttcacgacgt tcaacgttcg gcaggaactg gtacagcagg tggcacgccg gaagagctat ttcgtcaaga acagcagggt tccaaccgtg aaacaggata cgacggctcg acgggtcacg aactatagtg tccaggcaat cacgcagttg gtcgatcgct ttgtggagca gaactttctg ggcaccatct tcatcaaagc gagccacagc gcggaaaccg cggctagtag catcagctat ctgagcgtcg gcgtgggcgt cgaggacctt gtaacctggc caacggcgcc attgcacacc atacacgtca cccagatcat caacgccacg gcggtatggg ttagctaacc actgagcatt ctcgttagcg ttagcgtccc agactttaca gcccagggca cggaagtcag cagcggaacg ggtagcacgc aggaaaccaa ctaattcgcc gattgcggat ttgaatgcga gcttcttcgt agtcacggcc gggaaaccct gttggaggtc aaaacggagc atagcgcctg ggatgcctat agtacggata ccggtacggt tagactgcca agtaccggtg tccaggatgg tacgaactaa gtcaaggtat ccaccgattt gttcacggtg tgcttcgata atgtcgttat cttccatgtc cagatcttcc ggagtctggt ctgcctgaat acggataccg tcgtacagaa agcgcaggga atccatttcc ttgccctggc gtttagcgaa tgcttccatc aggcgacgca gcggggtggt cttcttaatc ttgaagaaga tttcgctgct accgtcgcta accttcaggt tgatgtgggt ctccggcttc acttccggtt ttacttcgcc gcta
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYLDL VRTILDTGTW QSNRTGIRTI GIPGAMLRFD LQQGFPAVTT KKLAFKSAIG ELVGFLRATR SAADFRALGC KVWDANANEN AQWLANPYRR GVDDLGDVYG VQWRRWPGYK VLDAHADAQI ADATSRGFRA VARFDEDGAQ KVLLHKAIDQ LRDCLDTIVR DPSSRRILFH GWNPAVLDEI ALPACHLLYQ FLPNVERREI SLCLYIRSND VGLGTPFNLA EGAALLTLVG RLTGYTPRWF TYFIGDAHIY ENQLGMLKQQ LEREPFDSPR LEIAERVPEY AKTGQYEPQW LERIEPSDFT LVGYRHHE
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata ccttgactta gttcgtacca tcctggacac cggtacttgg cagtctaacc gtaccggtat ccgtactata ggcatcccag gcgctatgct ccgttttgac ctccaacagg gtttcccggc cgtgactacg aagaagctcg cattcaaatc cgcaatcggc gaattagttg gtttcctgcg tgctacccgt tccgctgctg acttccgtgc cctgggctgt aaagtctggg acgctaacgc taacgagaat gctcagtggt tagctaaccc ataccgccgt ggcgttgatg atctgggtga cgtgtatggt gtgcaatggc gccgttggcc aggttacaag gtcctcgacg cccacgccga cgctcagata gctgatgcta ctagccgcgg tttccgcgct gtggctcgct ttgatgaaga tggtgcccag aaagttctgc tccacaaagc gatcgaccaa ctgcgtgatt gcctggacac tatagttcgt gacccgtcga gccgtcgtat cctgtttcac ggttggaacc ctgctgttct tgacgaaata gctcttccgg cgtgccacct gctgtaccag ttcctgccga acgttgaacg tcgtgaaatt agcctgtgtc tgtatatccg ttctaacgac gtgggtttag gcactccgtt caacctggct gaaggtgctg cactcctgac cctggtaggc cgtcttactg gctacactcc gcgctggttt acttatttca taggcgatgc ccacatctat gaaaatcagc tgggtatgct gaagcagcag ctcgaacgtg aaccattcga ctctccacgc ctggaaatag ctgagcgcgt cccagaatac gctaaaactg gtcagtacga accacagtgg ctcgaacgca tcgagccgtc cgatttcacc ctggttggtt accgtcacca tgaataaaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggat
Details for BupsA.01181.a.D14.PD00061
PURIFICATION DATe: 5/27/2009
CONCENTRATION: 16mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 95
PERCENT COVERAGE: 69
Protocol Notes
notes unavailable
Validated AA Sequence
MXRFDLXQXX PXVXTKKLXF KSXXGELVGF XRATRSAADF RAXGCKVWDA NANENAQWLA NPYRRGVDDL GDVYGVQWRR WPGYKVLDAH ADAQIXDATS RGFRAVARFD EDGAQKVLLH KAIDQLRDCL DTIVRDPSSR RILFHGWNPA VLDEIALPAC HLLYQFLPNV ERREISLCLY IRSNDVGLGT PFNLAEGAAL LTLVGRLTGY TPRWFTYFIG DAHIYENQLG MLKQQLEREP FDSPRLEIAE RVPEYAKTGQ YEPQWLERIE PSDFTLVGYR HHEPLSAPMA V
Validated NT Sequence
gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc ttttacacag ccataggagc gctcagcggt tcatggtgac ggtaaccaac cagggtgaaa tcggacggct cgatgcgttc gagccactgt ggttcgtact gaccagtttt agcgtattct gggacgcgct cagctatttc caggcgtgga gagtcgaatg gttcacgttc gagctgctgc ttcagcatac ccagctgatt ttcatagatg tgggcatcgc ctatgaaata agtaaaccag cgcggagtgt agccagtaag acggcctacc agggtcagga gtgcagcacc ttcagccagg ttgaacggag tgcctaaacc cacgtcgtta gaacggatat acagacacag gctaatttca cgacgttcaa cgttcggcag gaactggtac agcaggtggc acgccggaag agctatttcg tcaagaacag cagggttcca accgtgaaac aggatacgac ggctcgacgg gtcacgaact atagtgtcca ggcaatcacg cagttggtcg atcgctttgt ggagcagaac tttctgggca ccatcttcat caaagcgagc cacagcgcgg aaaccgcggc tagtagcatc anctatctga gcgtcngcgt gggcgtcgag gaccttgtaa cctggccaac ggcgccattg cacaccatac acgtcaccca gatcatcnac gccacggcgg tatgggttag ctaaccactg agcattctcg ttagcgttag cgtcccagac tttacagccc nnggcacgga agtcagcagc ggaacgggta gcacgcnnga aaccaactaa ttcgccgann gnggatttga atgnnagctt cttcgtnntc acggncggnn aancctgntg gaggtcaaaa cggancatan cgcctgggnn gcntatanna cgnataccng nanggnnann ctnnnnngta nnngnntccn nnnnggnncg nactnagtcn nggnatcnat nnnnnanggn nnnntnnnna nnncntntnt nnnnnncnnn tnnnnnnnnc nnanncnnnn ncnnnnnnnn nannnnnnnn nntnnnnnnn nngnnnnnnn gnnnnnnnnn nnnnnctnnn nnnnnaanan tnnnnnnnnc nnnnnnnnaa
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYLDL VRTILDTGTW QSNRTGIRTI GIPGAMLRFD LQQGFPAVTT KKLAFKSAIG ELVGFLRATR SAADFRALGC KVWDANANEN AQWLANPYRR GVDDLGDVYG VQWRRWPGYK VLDAHADAQI ADATSRGFRA VARFDEDGAQ KVLLHKAIDQ LRDCLDTIVR DPSSRRILFH GWNPAVLDEI ALPACHLLYQ FLPNVERREI SLCLYIRSND VGLGTPFNLA EGAALLTLVG RLTGYTPRWF TYFIGDAHIY ENQLGMLKQQ LEREPFDSPR LEIAERVPEY AKTGQYEPQW LERIEPSDFT LVGYRHHEPL SAPMAV
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATA CCTTGACTTA GTTCGTACCA TCCTGGACAC CGGTACTTGG CAGTCTAACC GTACCGGTAT CCGTACTATA GGCATCCCAG GCGCTATGCT CCGTTTTGAC CTCCAACAGG GTTTCCCGGC CGTGACTACG AAGAAGCTCG CATTCAAATC CGCAATCGGC GAATTAGTTG GTTTCCTGCG TGCTACCCGT TCCGCTGCTG ACTTCCGTGC CCTGGGCTGT AAAGTCTGGG ACGCTAACGC TAACGAGAAT GCTCAGTGGT TAGCTAACCC ATACCGCCGT GGCGTTGATG ATCTGGGTGA CGTGTATGGT GTGCAATGGC GCCGTTGGCC AGGTTACAAG GTCCTCGACG CCCACGCCGA CGCTCAGATA GCTGATGCTA CTAGCCGCGG TTTCCGCGCT GTGGCTCGCT TTGATGAAGA TGGTGCCCAG AAAGTTCTGC TCCACAAAGC GATCGACCAA CTGCGTGATT GCCTGGACAC TATAGTTCGT GACCCGTCGA GCCGTCGTAT CCTGTTTCAC GGTTGGAACC CTGCTGTTCT TGACGAAATA GCTCTTCCGG CGTGCCACCT GCTGTACCAG TTCCTGCCGA ACGTTGAACG TCGTGAAATT AGCCTGTGTC TGTATATCCG TTCTAACGAC GTGGGTTTAG GCACTCCGTT CAACCTGGCT GAAGGTGCTG CACTCCTGAC CCTGGTAGGC CGTCTTACTG GCTACACTCC GCGCTGGTTT ACTTATTTCA TAGGCGATGC CCACATCTAT GAAAATCAGC TGGGTATGCT GAAGCAGCAG CTCGAACGTG AACCATTCGA CTCTCCACGC CTGGAAATAG CTGAGCGCGT CCCAGAATAC GCTAAAACTG GTCAGTACGA ACCACAGTGG CTCGAACGCA TCGAGCCGTC CGATTTCACC CTGGTTGGTT ACCGTCACCA TGAACCGCTG AGCGCTCCTA TGGCTGTGTA AAAGCTTGCG GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAT