BupsA.01155.a

HAD-superfamily subfamily IB hydrolase, TIGR01490

CENTER ID: BupsA.01155.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.01155.a.A1.GE32552 Full length( BupsA.01155.a ) 1 228
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.01155.a.A1.PS01138 Full length( BupsA.01155.a ) 1 228

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3452
RefSeq: YP_334684.1
UniProt: Q3JP19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNLALFDLD HTLIPTDSDH EWGRFMVRLG IVDADSFSRQ NDRFYADYKA GKLDIHAYLS AMLTPLAKYS RAQLAQWHDR YMHEVIRPAM LPAAIELVRR HQDAGDLCCI VTATNEFITR PIATAFGVDT LIACEVETVD GHPDSAFTGR PTGTPSYREG KITRTQAWLA SLGKRWDDFD RSYFYSDSHN DIPLLEKVTD PIATNPDDTL RAYAREHGWR ILDLFQPS
NT Sequence
ATGACTAATC TGGCACTTTT CGATCTGGAT CACACGCTGA TCCCCACCGA TAGCGATCAC GAGTGGGGCC GCTTCATGGT CCGGCTCGGC ATCGTCGACG CGGACAGCTT CTCGCGTCAG AACGACCGCT TCTACGCCGA CTACAAGGCC GGCAAGCTCG ACATCCACGC GTACCTGAGC GCGATGCTCA CGCCGCTCGC GAAGTATTCG CGCGCGCAGC TCGCGCAGTG GCACGACCGG TACATGCACG AAGTGATCCG GCCGGCGATG CTGCCCGCCG CGATCGAGCT CGTGCGCCGG CATCAGGACG CGGGCGACCT CTGCTGCATC GTCACCGCGA CGAACGAATT CATCACGCGT CCGATCGCGA CCGCGTTCGG CGTCGACACG CTGATCGCCT GCGAAGTGGA AACCGTCGAC GGCCATCCCG ATTCCGCGTT CACCGGCCGC CCGACGGGCA CGCCGAGCTA CCGCGAAGGC AAGATCACCC GCACGCAGGC GTGGCTCGCA TCGCTCGGCA AGCGCTGGGA CGATTTCGAC CGCAGCTATT TCTACAGCGA TTCGCACAAC GACATTCCGC TCCTCGAAAA AGTCACCGAC CCGATCGCGA CCAATCCCGA CGACACGCTG CGCGCATACG CACGCGAGCA CGGCTGGCGC ATCCTCGACC TCTTCCAACC GTCG
Details for BupsA.01155.a.A1.GE32552
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/24/2011
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMTNLALF DLDHTLIPTD SDHEWGRFMV RLGIVDADSF SRQNDRFYAD YKAGKLDIHA YLSAMLTPLA KYSRAQLAQW HDRYMHEVIR PAMLPAAIEL VRRHQDAGDL CCIVTATNEF ITRPIATAFG VDTLIACEVE TVDGHPDSAF TGRPTGTPSY REGKITRTQA WLASLGKRWD DFDRSYFYSD SHNDIPLLEK VTDPIATNPD DTLRAYAREH GWRILDLFQP S
Validated NT Sequence
ttattacgac ggttggaaga ggtcgaggat gcgccagccg tgctcgcgtg cgtatgcgcg cagcgtgtcg tcgggattgg tcgcgatcgg gtcggtgact ttttcgagga gcggaatgtc gttgtgcgaa tcgctgtaga aatagctgcg gtcgaaatcg tcccagcgct tgccgagcga tgcgagccac gcctgcgtgc gggtgatctt gccttcgcgg tagctcggcg tgcccgtcgg gcggccggtg aacgcggaat cgggatggcc gtcgacggtt tccacttcgc aggcgatcag cgtgtcgacg ccgaacgcgg tcgcgatcgg acgcgtgatg aattcgttcg tcgcggtgac gatgcagcag aggtcgcccg cgtcctgatg ccggcgcacg agctcgatcg cggcgggcag catcgccggc cggatcactt cgtgcatgta ccggtcgtgc cactgcgcga gctgcgcgcg cgaatacttc gcgagcggcg tgagcatcgc gctcaggtac gcgtggatgt cgagcttgcc ggccttgtag tcggcgtaga agcggtcgtt ctgacgcgag aagctgtccg cgtcgacgat gccgagccgg accatgaagc ggccccactc gtgatcgcta tcggtgggga tcagcgtgtg atccagatcg aaaagtgcca gattagtcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNLALFDL DHTLIPTDSD HEWGRFMVRL GIVDADSFSR QNDRFYADYK AGKLDIHAYL SAMLTPLAKY SRAQLAQWHD RYMHEVIRPA MLPAAIELVR RHQDAGDLCC IVTATNEFIT RPIATAFGVD TLIACEVETV DGHPDSAFTG RPTGTPSYRE GKITRTQAWL ASLGKRWDDF DRSYFYSDSH NDIPLLEKVT DPIATNPDDT LRAYAREHGW RILDLFQPS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactaatctg gcacttttcg atctggatca cacgctgatc cccaccgata gcgatcacga gtggggccgc ttcatggtcc ggctcggcat cgtcgacgcg gacagcttct cgcgtcagaa cgaccgcttc tacgccgact acaaggccgg caagctcgac atccacgcgt acctgagcgc gatgctcacg ccgctcgcga agtattcgcg cgcgcagctc gcgcagtggc acgaccggta catgcacgaa gtgatccggc cggcgatgct gcccgccgcg atcgagctcg tgcgccggca tcaggacgcg ggcgacctct gctgcatcgt caccgcgacg aacgaattca tcacgcgtcc gatcgcgacc gcgttcggcg tcgacacgct gatcgcctgc gaagtggaaa ccgtcgacgg ccatcccgat tccgcgttca ccggccgccc gacgggcacg ccgagctacc gcgaaggcaa gatcacccgc acgcaggcgt ggctcgcatc gctcggcaag cgctgggacg atttcgaccg cagctatttc tacagcgatt cgcacaacga cattccgctc ctcgaaaaag tcaccgaccc gatcgcgacc aatcccgacg acacgctgcg cgcatacgca cgcgagcacg gctggcgcat cctcgacctc ttccaaccgt cgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BupsA.01155.a.A1.PS01138
PURIFICATION DATe: 7/1/2011
CONCENTRATION: 54.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTNLALF DLDHTLIPTD SDHEWGRFMV RLGIVDADSF SRQNDRFYAD YKAGKLDIHA YLSAMLTPLA KYSRAQLAQW HDRYMHEVIR PAMLPAAIEL VRRHQDAGDL CCIVTATNEF ITRPIATAFG VDTLIACEVE TVDGHPDSAF TGRPTGTPSY REGKITRTQA WLASLGKRWD DFDRSYFYSD SHNDIPLLEK VTDPIATNPD DTLRAYAREH GWRILDLFQP S
Validated NT Sequence
ttattacgac ggttggaaga ggtcgaggat gcgccagccg tgctcgcgtg cgtatgcgcg cagcgtgtcg tcgggattgg tcgcgatcgg gtcggtgact ttttcgagga gcggaatgtc gttgtgcgaa tcgctgtaga aatagctgcg gtcgaaatcg tcccagcgct tgccgagcga tgcgagccac gcctgcgtgc gggtgatctt gccttcgcgg tagctcggcg tgcccgtcgg gcggccggtg aacgcggaat cgggatggcc gtcgacggtt tccacttcgc aggcgatcag cgtgtcgacg ccgaacgcgg tcgcgatcgg acgcgtgatg aattcgttcg tcgcggtgac gatgcagcag aggtcgcccg cgtcctgatg ccggcgcacg agctcgatcg cggcgggcag catcgccggc cggatcactt cgtgcatgta ccggtcgtgc cactgcgcga gctgcgcgcg cgaatacttc gcgagcggcg tgagcatcgc gctcaggtac gcgtggatgt cgagcttgcc ggccttgtag tcggcgtaga agcggtcgtt ctgacgcgag aagctgtccg cgtcgacgat gccgagccgg accatgaagc ggccccactc gtgatcgcta tcggtgggga tcagcgtgtg atccagatcg aaaagtgcca gattagtcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat gg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNLALFDL DHTLIPTDSD HEWGRFMVRL GIVDADSFSR QNDRFYADYK AGKLDIHAYL SAMLTPLAKY SRAQLAQWHD RYMHEVIRPA MLPAAIELVR RHQDAGDLCC IVTATNEFIT RPIATAFGVD TLIACEVETV DGHPDSAFTG RPTGTPSYRE GKITRTQAWL ASLGKRWDDF DRSYFYSDSH NDIPLLEKVT DPIATNPDDT LRAYAREHGW RILDLFQPS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTAATCTG GCACTTTTCG ATCTGGATCA CACGCTGATC CCCACCGATA GCGATCACGA GTGGGGCCGC TTCATGGTCC GGCTCGGCAT CGTCGACGCG GACAGCTTCT CGCGTCAGAA CGACCGCTTC TACGCCGACT ACAAGGCCGG CAAGCTCGAC ATCCACGCGT ACCTGAGCGC GATGCTCACG CCGCTCGCGA AGTATTCGCG CGCGCAGCTC GCGCAGTGGC ACGACCGGTA CATGCACGAA GTGATCCGGC CGGCGATGCT GCCCGCCGCG ATCGAGCTCG TGCGCCGGCA TCAGGACGCG GGCGACCTCT GCTGCATCGT CACCGCGACG AACGAATTCA TCACGCGTCC GATCGCGACC GCGTTCGGCG TCGACACGCT GATCGCCTGC GAAGTGGAAA CCGTCGACGG CCATCCCGAT TCCGCGTTCA CCGGCCGCCC GACGGGCACG CCGAGCTACC GCGAAGGCAA GATCACCCGC ACGCAGGCGT GGCTCGCATC GCTCGGCAAG CGCTGGGACG ATTTCGACCG CAGCTATTTC TACAGCGATT CGCACAACGA CATTCCGCTC CTCGAAAAAG TCACCGACCC GATCGCGACC AATCCCGACG ACACGCTGCG CGCATACGCA CGCGAGCACG GCTGGCGCAT CCTCGACCTC TTCCAACCGT CGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA