BupsA.01122.a
Ribosomal RNA small subunit methyltransferase A
| CENTER ID: | BupsA.01122.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.01122.a.A1.GE32550 | Full length( BupsA.01122.a ) | 1 | 275 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.01122.a.A1.PS01140 | Full length( BupsA.01122.a ) | 1 | 275 |
Structures
3UZU
DEPOSITED: 12/7/2011
DETERMINATION: XRay
CLONE: BupsA.01122.a.A1.GE32550
PROTEIN: BupsA.01122.a.A1.PS01140
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126745 |
| PATRIC ID: | fig|320372.6.peg.883 |
| RefSeq: | YP_332287.1 |
| UniProt: | Q3JVW6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNSRQHQGH FARKRFGQNF LVDHGVIDAI VAAIRPERGE RMVEIGPGLG ALTGPVIARL ATPGSPLHAV ELDRDLIGRL EQRFGELLEL HAGDALTFDF GSIARPGDEP SLRIIGNLPY NISSPLLFHL MSFAPVVIDQ HFMLQNEVVE RMVAEPGTKA FSRLSVMLQY RYVMDKLIDV PPESFQPPPK VDSAIVRMIP HAPHELPAVD PAVLGEVVTA AFSQRRKMLR NTLGGYRDLV DFDALGFDLA RRAEDIGVDE YVRVAQAVAS ARASG
NT Sequence
ATGTCGAACA GCAGACAGCA CCAAGGGCAT TTCGCGCGCA AGCGCTTCGG GCAGAACTTT CTCGTCGATC ACGGTGTGAT CGATGCGATC GTCGCGGCGA TTCGTCCCGA GCGCGGCGAG CGGATGGTCG AGATCGGGCC GGGCCTCGGC GCGCTCACCG GGCCCGTGAT CGCGCGGCTC GCGACGCCCG GCTCGCCGCT GCACGCGGTC GAGCTCGACC GCGACTTGAT CGGCCGCCTC GAGCAGCGCT TCGGCGAGCT GCTCGAGCTG CACGCGGGCG ACGCGCTCAC GTTCGACTTC GGCTCAATCG CGCGGCCGGG CGACGAGCCG TCGCTGCGGA TCATCGGCAA CCTGCCGTAC AACATCTCGA GCCCGCTGCT GTTCCATCTG ATGTCGTTCG CGCCCGTCGT GATCGATCAG CACTTCATGC TGCAGAACGA GGTGGTCGAG CGGATGGTCG CCGAACCGGG CACGAAGGCG TTCAGCCGGC TGTCGGTGAT GCTTCAGTAC CGCTACGTGA TGGACAAGCT GATCGACGTG CCGCCGGAGT CGTTCCAGCC GCCGCCGAAG GTCGATTCGG CGATCGTGCG GATGATCCCG CATGCGCCGC ACGAGTTGCC GGCCGTCGAT CCGGCGGTGC TCGGCGAAGT CGTGACGGCT GCGTTCTCGC AGCGCCGCAA GATGCTGCGC AACACGCTCG GCGGGTATCG CGATCTCGTC GATTTCGATG CGCTCGGCTT CGATCTCGCG CGCCGCGCGG AAGACATCGG TGTCGACGAG TACGTGCGCG TCGCACAGGC GGTGGCTTCG GCGCGGGCGA GCGGC
Details for BupsA.01122.a.A1.GE32550
| HARVESTED ON: | 5/17/2011 |
| SEQUENCED ON: | 5/23/2011 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Validated AA Sequence
MSNSRQHQGH FARKRFGQNF LVDHGVIDAI VAAIRPERGE RMVEIGPGLG ALTGPVIARL ATPGSPLHAV ELDRDLIGRL EQRFGELLEL HAGDALTFDF GSIARPGDEP SLRIIGNLPY NISSPLLFHL MSFAPVVIDQ HFMLQNEVVE RMVAEPGTKA FSRLSVMLQY RYVMDKLIDV PPESFQPPPK VDSAIVRMIP HAPHELPAVD PAVLGEVVTA AFSQRRKMLR NTLGGYRDLV DFDALGFDLA RRAEDIGVDE YVRVAQAVAS ARASG
Validated NT Sequence
ttattagccg ctcgcccgcg ccgaagccac cgcctgtgcg acgcgcacgt actcgtcgac accgatgtct tccgcgcggc gcgcgagatc gaagccgagc gcatcgaaat cgacgagatc gcgatacccg ccgagcgtgt tgcgcagcat cttgcggcgc tgcgagaacg cagccgtcac gacttcgccg agcaccgccg gatcgacggc cggcaactcg tgcggcgcat gcgggatcat ccgcacgatc gccgaatcga ccttcggcgg cggctggaac gactccggcg gcacgtcgat cagcttgtcc atcacgtagc ggtactgaag catcaccgac agccggctga acgccttcgt gcccggttcg gcgaccatcc gctcgaccac ctcgttctgc agcatgaagt gctgatcgat cacgacgggc gcgaacgaca tcagatggaa cagcagcggg ctcgagatgt tgtacggcag gttgccgatg atccgcagcg acggctcgtc gcccggccgc gcgattgagc cgaagtcgaa cgtgagcgcg tcgcccgcgt gcagctcgag cagctcgccg aagcgctgct cgaggcggcc gatcaagtcg cggtcgagct cgaccgcgtg cagcggcgag ccgggcgtcg cgagccgcgc gatcacgggc ccggtgagcg cgccgaggcc cggcccgatc tcgaccatcc gctcgccgcg ctcgggacga atcgccgcga cgatcgcatc gatcacaccg tgatcgacga gaaagttctg cccgaagcgc ttgcgcgcga aatgcccttg gtgctgtctg ctgttcgaca tcgaaccagg accctgggtc tgagcttcca ggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNSRQHQG HFARKRFGQN FLVDHGVIDA IVAAIRPERG ERMVEIGPGL GALTGPVIAR LATPGSPLHA VELDRDLIGR LEQRFGELLE LHAGDALTFD FGSIARPGDE PSLRIIGNLP YNISSPLLFH LMSFAPVVID QHFMLQNEVV ERMVAEPGTK AFSRLSVMLQ YRYVMDKLID VPPESFQPPP KVDSAIVRMI PHAPHELPAV DPAVLGEVVT AAFSQRRKML RNTLGGYRDL VDFDALGFDL ARRAEDIGVD EYVRVAQAVA SARASG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgaacagc agacagcacc aagggcattt cgcgcgcaag cgcttcgggc agaactttct cgtcgatcac ggtgtgatcg atgcgatcgt cgcggcgatt cgtcccgagc gcggcgagcg gatggtcgag atcgggccgg gcctcggcgc gctcaccggg cccgtgatcg cgcggctcgc gacgcccggc tcgccgctgc acgcggtcga gctcgaccgc gacttgatcg gccgcctcga gcagcgcttc ggcgagctgc tcgagctgca cgcgggcgac gcgctcacgt tcgacttcgg ctcaatcgcg cggccgggcg acgagccgtc gctgcggatc atcggcaacc tgccgtacaa catctcgagc ccgctgctgt tccatctgat gtcgttcgcg cccgtcgtga tcgatcagca cttcatgctg cagaacgagg tggtcgagcg gatggtcgcc gaaccgggca cgaaggcgtt cagccggctg tcggtgatgc ttcagtaccg ctacgtgatg gacaagctga tcgacgtgcc gccggagtcg ttccagccgc cgccgaaggt cgattcggcg atcgtgcgga tgatcccgca tgcgccgcac gagttgccgg ccgtcgatcc ggcggtgctc ggcgaagtcg tgacggctgc gttctcgcag cgccgcaaga tgctgcgcaa cacgctcggc gggtatcgcg atctcgtcga tttcgatgcg ctcggcttcg atctcgcgcg ccgcgcggaa gacatcggtg tcgacgagta cgtgcgcgtc gcacaggcgg tggcttcggc gcgggcgagc ggctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BupsA.01122.a.A1.PS01140
| PURIFICATION DATe: | 6/29/2011 |
| CONCENTRATION: | 47.135mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSNSRQHQGH FARKRFGQNF LVDHGVIDAI VAAIRPERGE RMVEIGPGLG ALTGPVIARL ATPGSPLHAV ELDRDLIGRL EQRFGELLEL HAGDALTFDF GSIARPGDEP SLRIIGNLPY NISSPLLFHL MSFAPVVIDQ HFMLQNEVVE RMVAEPGTKA FSRLSVMLQY RYVMDKLIDV PPESFQPPPK VDSAIVRMIP HAPHELPAVD PAVLGEVVTA AFSQRRKMLR NTLGGYRDLV DFDALGFDLA RRAEDIGVDE YVRVAQAVAS ARASG
Validated NT Sequence
ttattagccg ctcgcccgcg ccgaagccac cgcctgtgcg acgcgcacgt actcgtcgac accgatgtct tccgcgcggc gcgcgagatc gaagccgagc gcatcgaaat cgacgagatc gcgatacccg ccgagcgtgt tgcgcagcat cttgcggcgc tgcgagaacg cagccgtcac gacttcgccg agcaccgccg gatcgacggc cggcaactcg tgcggcgcat gcgggatcat ccgcacgatc gccgaatcga ccttcggcgg cggctggaac gactccggcg gcacgtcgat cagcttgtcc atcacgtagc ggtactgaag catcaccgac agccggctga acgccttcgt gcccggttcg gcgaccatcc gctcgaccac ctcgttctgc agcatgaagt gctgatcgat cacgacgggc gcgaacgaca tcagatggaa cagcagcggg ctcgagatgt tgtacggcag gttgccgatg atccgcagcg acggctcgtc gcccggccgc gcgattgagc cgaagtcgaa cgtgagcgcg tcgcccgcgt gcagctcgag cagctcgccg aagcgctgct cgaggcggcc gatcaagtcg cggtcgagct cgaccgcgtg cagcggcgag ccgggcgtcg cgagccgcgc gatcacgggc ccggtgagcg cgccgaggcc cggcccgatc tcgaccatcc gctcgccgcg ctcgggacga atcgccgcga cgatcgcatc gatcacaccg tgatcgacga gaaagttctg cccgaagcgc ttgcgcgcga aatgcccttg gtgctgtctg ctgttcgaca tcgaaccagg accctgggtc tgagcttcca ggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNSRQHQG HFARKRFGQN FLVDHGVIDA IVAAIRPERG ERMVEIGPGL GALTGPVIAR LATPGSPLHA VELDRDLIGR LEQRFGELLE LHAGDALTFD FGSIARPGDE PSLRIIGNLP YNISSPLLFH LMSFAPVVID QHFMLQNEVV ERMVAEPGTK AFSRLSVMLQ YRYVMDKLID VPPESFQPPP KVDSAIVRMI PHAPHELPAV DPAVLGEVVT AAFSQRRKML RNTLGGYRDL VDFDALGFDL ARRAEDIGVD EYVRVAQAVA SARASG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGAACAGC AGACAGCACC AAGGGCATTT CGCGCGCAAG CGCTTCGGGC AGAACTTTCT CGTCGATCAC GGTGTGATCG ATGCGATCGT CGCGGCGATT CGTCCCGAGC GCGGCGAGCG GATGGTCGAG ATCGGGCCGG GCCTCGGCGC GCTCACCGGG CCCGTGATCG CGCGGCTCGC GACGCCCGGC TCGCCGCTGC ACGCGGTCGA GCTCGACCGC GACTTGATCG GCCGCCTCGA GCAGCGCTTC GGCGAGCTGC TCGAGCTGCA CGCGGGCGAC GCGCTCACGT TCGACTTCGG CTCAATCGCG CGGCCGGGCG ACGAGCCGTC GCTGCGGATC ATCGGCAACC TGCCGTACAA CATCTCGAGC CCGCTGCTGT TCCATCTGAT GTCGTTCGCG CCCGTCGTGA TCGATCAGCA CTTCATGCTG CAGAACGAGG TGGTCGAGCG GATGGTCGCC GAACCGGGCA CGAAGGCGTT CAGCCGGCTG TCGGTGATGC TTCAGTACCG CTACGTGATG GACAAGCTGA TCGACGTGCC GCCGGAGTCG TTCCAGCCGC CGCCGAAGGT CGATTCGGCG ATCGTGCGGA TGATCCCGCA TGCGCCGCAC GAGTTGCCGG CCGTCGATCC GGCGGTGCTC GGCGAAGTCG TGACGGCTGC GTTCTCGCAG CGCCGCAAGA TGCTGCGCAA CACGCTCGGC GGGTATCGCG ATCTCGTCGA TTTCGATGCG CTCGGCTTCG ATCTCGCGCG CCGCGCGGAA GACATCGGTG TCGACGAGTA CGTGCGCGTC GCACAGGCGG TGGCTTCGGC GCGGGCGAGC GGCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA