BupsA.00952.a

UDP-N-acetylglucosamine 1-carboxyvinyltransferase, putative

CENTER ID: BupsA.00952.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00952.a.A1.GE30924 Full length( BupsA.00952.a ) 1 517
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.6813
RefSeq: YP_337746.1
UniProt: Q3JFA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRLAWKGYD RQCRAQCDHP SSRDSCEAPG LIPPYRRLIA RIVVPLAPPV SPGRPSRRAT NLRNPRDRWH DIDLARHYTH LHPNMSNLIV HGGAPLRGEI TPSANKNAVL PILCATLLTD RPLRLVGVPD ITDVRKILDI FRTLGSDVSI DYASGVLDLH HRATAFDPAV HRLPEEMRSS IMLVPPLLAR FGVARLENDV KGCTLGVREI DPHVEVFERF GARIERTSDS LIVRADGPLT PNHHWLDYAS VTTTENFVLC AASANGTSTL VNAASEPHVQ EFCRFLAMLG VPIEGIGTSH LSVQGGRALA GGEYRFNEDF HEIATFLALG AITGGDIAVR NGSPEQFPLI DRTFAKFGVQ VTHENGWSHA LRDGPLKVKQ PFTRNILTKV EAAPWPYLPV DLLPIFIALG VQAQGSVMFW NKVYDGAMGW TGELSKFGAH VFLSDPHRLI TFGGLPLSPA RVESPYIIRV AIALLMVAAS IDGRSEILNA QPIRRAHPHF VENLRSVGAN VEWTSGE
NT Sequence
ATGCGTCGCC TCGCTTGGAA AGGGTACGAT CGTCAATGCC GCGCGCAATG CGATCATCCC TCTTCGCGCG ATTCATGTGA AGCGCCCGGC CTTATCCCAC CTTATCGCCG CCTTATCGCC CGTATCGTCG TCCCCCTCGC GCCGCCCGTA TCGCCCGGCC GGCCCTCGCG CCGCGCGACG AATCTGCGAA ATCCTCGCGA TCGGTGGCAT GATATCGACC TTGCGCGTCA TTACACCCAC CTGCATCCGA ACATGTCGAA CCTGATCGTC CACGGCGGCG CCCCGCTTCG CGGAGAAATC ACGCCGTCCG CCAACAAGAA CGCCGTCCTG CCCATCCTGT GCGCGACGCT CCTCACCGAC CGGCCGCTGC GGCTCGTCGG CGTGCCGGAC ATCACCGACG TGCGCAAGAT CCTCGACATC TTCCGCACGC TCGGCAGCGA CGTCTCGATC GATTACGCGA GCGGCGTGCT CGATCTGCAC CATCGCGCGA CCGCGTTCGA TCCGGCCGTC CACCGGCTGC CGGAGGAGAT GCGCTCGTCG ATCATGCTGG TGCCGCCGCT GCTCGCGCGC TTCGGCGTCG CGCGGCTCGA GAACGACGTA AAGGGCTGCA CGCTCGGCGT GCGCGAGATC GATCCGCACG TCGAAGTGTT CGAGCGCTTC GGCGCGCGCA TCGAGCGCAC GTCCGATTCG CTGATCGTGC GCGCCGACGG CCCGCTCACG CCGAATCATC ACTGGCTCGA CTACGCGTCC GTCACGACGA CCGAGAACTT CGTGCTGTGC GCCGCGTCGG CGAACGGCAC GTCGACGCTC GTCAATGCCG CGTCGGAGCC GCACGTGCAG GAGTTCTGCC GGTTCCTCGC GATGCTCGGC GTGCCGATCG AGGGCATCGG CACGTCGCAC CTGAGCGTTC AGGGCGGGCG CGCGCTCGCG GGCGGCGAAT ACCGCTTCAA CGAGGACTTT CACGAAATCG CGACGTTTCT CGCGCTCGGC GCGATCACGG GCGGCGACAT CGCGGTGCGT AACGGCTCGC CCGAGCAGTT TCCGCTGATC GATCGGACCT TCGCGAAATT CGGCGTGCAG GTCACGCACG AGAACGGCTG GTCGCACGCG CTGCGCGACG GCCCGCTGAA GGTCAAGCAG CCGTTCACGC GCAACATCCT GACGAAAGTC GAGGCCGCGC CGTGGCCCTA CCTGCCCGTC GATCTGCTGC CGATCTTCAT CGCGCTCGGC GTGCAGGCGC AAGGCAGCGT GATGTTCTGG AACAAGGTGT ATGACGGCGC GATGGGCTGG ACGGGCGAGC TGTCGAAGTT CGGCGCGCAC GTGTTCCTGT CCGATCCGCA TCGGCTGATC ACGTTCGGCG GGCTGCCGCT CAGCCCGGCG CGCGTCGAGA GCCCGTACAT CATCCGCGTC GCGATCGCGC TGCTGATGGT CGCCGCGAGC ATCGACGGAC GCTCGGAGAT CCTGAACGCA CAGCCGATCC GGCGCGCGCA TCCGCACTTC GTCGAGAACC TGCGCTCGGT CGGCGCGAAC GTCGAGTGGA CGAGCGGCGA A
Details for BupsA.00952.a.A1.GE30924
HARVESTED ON: 11/23/2010
SEQUENCED ON: 12/6/2010
EXPECTED MW: 59kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 44
PERCENT COVERAGE: 37
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRRLAWKGY DRQCRXXXXX XSSRDXXEAP XLSHLIXAXX XVXXSPSRRX XXPAGXXXXX XXCKXXXIGG XXSXLARHYT HLHPNMSNLI VHXGAPLXGX ITPSANKNAV LPXXARXSXX TGXXGXXXCX XXPXCXXXST SFXXXXXVSX XYXSXVXXXX XXXXXXXXXX XRXXXXXXXX XX
Validated NT Sequence
antannnnnn annactttaa gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg cgtcgcctcg cttggaaagg gtacgatcgt caatgccgct nannnnnnnc tnncanctct tcgcgcgatt nnngngaagc gccgnnntta tcccacctta tcgncgcnnn atngnnngta ncancntccc cctcgcgccg nncgnntcnc ccggccggcc ntnnnnncgn gnnacnaanc tgcaaaatnn tcnngatcgg tggcannntn tcgnancttg cgcgtcatta cacccacctg catccgaaca tgtcgaacct gatcgtccac ngcggcgccc cgcttcncgg anaaatcacg ccgtccgcca acaagaacgc cgtcctgccn ancngngcgc gangctcctn nncnaccggn ngnngcggcn nnncggnntg ccnnannncn ccnanntgcg nnnnancctc nacatctttc nncannntnn ncnacgtctc gatnnantac gnnagcgncg tgnnnnatcn gncnngannn nnnnnnancn nnncnnnnnn cnnnngncgg annanangcn ctcntcnann nnnnnnnngc t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRRLAWKGY DRQCRAQCDH PSSRDSCEAP GLIPPYRRLI ARIVVPLAPP VSPGRPSRRA TNLRNPRDRW HDIDLARHYT HLHPNMSNLI VHGGAPLRGE ITPSANKNAV LPILCATLLT DRPLRLVGVP DITDVRKILD IFRTLGSDVS IDYASGVLDL HHRATAFDPA VHRLPEEMRS SIMLVPPLLA RFGVARLEND VKGCTLGVRE IDPHVEVFER FGARIERTSD SLIVRADGPL TPNHHWLDYA SVTTTENFVL CAASANGTST LVNAASEPHV QEFCRFLAML GVPIEGIGTS HLSVQGGRAL AGGEYRFNED FHEIATFLAL GAITGGDIAV RNGSPEQFPL IDRTFAKFGV QVTHENGWSH ALRDGPLKVK QPFTRNILTK VEAAPWPYLP VDLLPIFIAL GVQAQGSVMF WNKVYDGAMG WTGELSKFGA HVFLSDPHRL ITFGGLPLSP ARVESPYIIR VAIALLMVAA SIDGRSEILN AQPIRRAHPH FVENLRSVGA NVEWTSGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtcgcctc gcttggaaag ggtacgatcg tcaatgccgc gcgcaatgcg atcatccctc ttcgcgcgat tcatgtgaag cgcccggcct tatcccacct tatcgccgcc ttatcgcccg tatcgtcgtc cccctcgcgc cgcccgtatc gcccggccgg ccctcgcgcc gcgcgacgaa tctgcgaaat cctcgcgatc ggtggcatga tatcgacctt gcgcgtcatt acacccacct gcatccgaac atgtcgaacc tgatcgtcca cggcggcgcc ccgcttcgcg gagaaatcac gccgtccgcc aacaagaacg ccgtcctgcc catcctgtgc gcgacgctcc tcaccgaccg gccgctgcgg ctcgtcggcg tgccggacat caccgacgtg cgcaagatcc tcgacatctt ccgcacgctc ggcagcgacg tctcgatcga ttacgcgagc ggcgtgctcg atctgcacca tcgcgcgacc gcgttcgatc cggccgtcca ccggctgccg gaggagatgc gctcgtcgat catgctggtg ccgccgctgc tcgcgcgctt cggcgtcgcg cggctcgaga acgacgtaaa gggctgcacg ctcggcgtgc gcgagatcga tccgcacgtc gaagtgttcg agcgcttcgg cgcgcgcatc gagcgcacgt ccgattcgct gatcgtgcgc gccgacggcc cgctcacgcc gaatcatcac tggctcgact acgcgtccgt cacgacgacc gagaacttcg tgctgtgcgc cgcgtcggcg aacggcacgt cgacgctcgt caatgccgcg tcggagccgc acgtgcagga gttctgccgg ttcctcgcga tgctcggcgt gccgatcgag ggcatcggca cgtcgcacct gagcgttcag ggcgggcgcg cgctcgcggg cggcgaatac cgcttcaacg aggactttca cgaaatcgcg acgtttctcg cgctcggcgc gatcacgggc ggcgacatcg cggtgcgtaa cggctcgccc gagcagtttc cgctgatcga tcggaccttc gcgaaattcg gcgtgcaggt cacgcacgag aacggctggt cgcacgcgct gcgcgacggc ccgctgaagg tcaagcagcc gttcacgcgc aacatcctga cgaaagtcga ggccgcgccg tggccctacc tgcccgtcga tctgctgccg atcttcatcg cgctcggcgt gcaggcgcaa ggcagcgtga tgttctggaa caaggtgtat gacggcgcga tgggctggac gggcgagctg tcgaagttcg gcgcgcacgt gttcctgtcc gatccgcatc ggctgatcac gttcggcggg ctgccgctca gcccggcgcg cgtcgagagc ccgtacatca tccgcgtcgc gatcgcgctg ctgatggtcg ccgcgagcat cgacggacgc tcggagatcc tgaacgcaca gccgatccgg cgcgcgcatc cgcacttcgt cgagaacctg cgctcggtcg gcgcgaacgt cgagtggacg agcggcgaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa