SSGCID: Target Page

BupsA.00940.a

Undecaprenyl diphosphate synthase

CENTER ID:	BupsA.00940.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00940.a.A1.GE30921	Full length( BupsA.00940.a )		1	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.2704
RefSeq:	YP_333971.1
UniProt:	Q3JR32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTYTSSTVRV PDVAAVPRHI AIIMDGNGRW ATERRLPRVA GHTRGVDAVR SVVEGCARAG VEYLTLFAFS SENWRRPNDE VSFLMRLFIT ALEREVGKLH ANGIRLRVVG DLTKFEPRIQ ALIRRGETKT ARNTRLTLTI AANYGGRWDI LQATRKLVEE AAREGRAPEI DEDAFARHLA MAYAPEPDLF IRTGGEQRIS NFLLWQLAYT EFYFTDTFWP DFDAGALAEA LASYTHRERR FGRTSAQLEP QAQDADSLSC

NT Sequence

ATGACCTATA CCAGCTCTAC CGTTCGCGTG CCTGACGTCG CCGCCGTGCC GCGTCACATC GCGATCATCA TGGACGGCAA CGGCCGTTGG GCGACCGAGC GCCGCCTGCC GCGCGTCGCG GGGCACACGC GCGGCGTCGA CGCGGTGCGC TCGGTCGTCG AGGGATGCGC ACGCGCGGGC GTCGAATATC TGACGCTCTT CGCGTTCAGC TCCGAGAACT GGCGCCGGCC GAACGACGAA GTGTCGTTCC TGATGCGCCT GTTCATCACC GCGCTCGAGC GCGAGGTCGG CAAGCTGCAC GCGAACGGCA TCCGGCTGCG CGTCGTCGGC GATCTGACGA AGTTCGAGCC GCGCATCCAG GCGCTGATTC GTCGCGGCGA GACGAAGACG GCGCGCAATA CGCGCCTCAC GCTCACGATC GCCGCGAACT ACGGCGGCCG TTGGGACATC CTGCAGGCGA CGCGCAAGCT CGTCGAAGAG GCGGCGCGCG AAGGGCGCGC GCCCGAAATC GACGAGGACG CGTTCGCCCG CCATCTGGCG ATGGCCTATG CACCCGAGCC GGATCTCTTC ATCCGCACGG GCGGCGAGCA GCGCATCAGC AATTTCCTGC TCTGGCAGCT CGCCTATACC GAGTTCTACT TCACCGACAC GTTCTGGCCG GATTTCGACG CCGGCGCGCT CGCCGAAGCG CTCGCGTCGT ACACGCACCG CGAACGCCGC TTCGGCCGCA CGAGCGCGCA GCTCGAACCC CAGGCCCAGG ACGCCGATTC CCTTTCATGC

Details for BupsA.00940.a.A1.GE30921

HARVESTED ON:	11/23/2010
SEQUENCED ON:	12/6/2010
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTYTSSTVR VPDVAAVPRH IAIIMDGNGR WATERRLPRV AGHTRGVDAV RSVVEGCARA GVEYLTLFAF SSENWRRPND EVSFLMRLFI TALEREVGKL HANGIRLRVV GDLTKFEPRI QALIRRGETK TARNTRLTLT IAANYGGRWD ILQATRKLVE EAAREGRAPE IDEDAFARHL AMAYAPEPDL FIRTGGEQRI SNFLLWQLAY TEFYFTDTFW PDFDAGALAE ALASYTHRER RFGRTSAQLE PQAQDADSLS C

Validated NT Sequence

ttattagcat gaaagggaat cggcgtcctg ggcctggggt tcgagctgcg cgctcgtgcg gccgaagcgg cgttcgcggt gcgtgtacga cgcgagcgct tcggcgagcg cgccggcgtc gaaatccggc cagaacgtgt cggtgaagta gaactcggta taggcgagct gccagagcag gaaattgctg atgcgctgct cgccgcccgt gcggatgaag agatccggct cgggtgcata ggccatcgcc agatggcggg cgaacgcgtc ctcgtcgatt tcgggcgcgc gcccttcgcg cgccgcctct tcgacgagct tgcgcgtcgc ctgcaggatg tcccaacggc cgccgtagtt cgcggcgatc gtgagcgtga ggcgcgtatt gcgcgccgtc ttcgtctcgc cgcgacgaat cagcgcctgg atgcgcggct cgaacttcgt cagatcgccg acgacgcgca gccggatgcc gttcgcgtgc agcttgccga cctcgcgctc gagcgcggtg atgaacaggc gcatcaggaa cgacacttcg tcgttcggcc ggcgccagtt ctcggagctg aacgcgaaga gcgtcagata ttcgacgccc gcgcgtgcgc atccctcgac gaccgagcgc accgcgtcga cgccgcgcgt gtgccccgcg acgcgcggca ggcggcgctc ggtcgcccaa cggccgttgc cgtccatgat gatcgcgatg tgacgcggca cggcggcgac gtcaggcacg cgaacggtag agctggtata ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacctatacc agctctaccg ttcgcgtgcc tgacgtcgcc gccgtgccgc gtcacatcgc gatcatcatg gacggcaacg gccgttgggc gaccgagcgc cgcctgccgc gcgtcgcggg gcacacgcgc ggcgtcgacg cggtgcgctc ggtcgtcgag ggatgcgcac gcgcgggcgt cgaatatctg acgctcttcg cgttcagctc cgagaactgg cgccggccga acgacgaagt gtcgttcctg atgcgcctgt tcatcaccgc gctcgagcgc gaggtcggca agctgcacgc gaacggcatc cggctgcgcg tcgtcggcga tctgacgaag ttcgagccgc gcatccaggc gctgattcgt cgcggcgaga cgaagacggc gcgcaatacg cgcctcacgc tcacgatcgc cgcgaactac ggcggccgtt gggacatcct gcaggcgacg cgcaagctcg tcgaagaggc ggcgcgcgaa gggcgcgcgc ccgaaatcga cgaggacgcg ttcgcccgcc atctggcgat ggcctatgca cccgagccgg atctcttcat ccgcacgggc ggcgagcagc gcatcagcaa tttcctgctc tggcagctcg cctataccga gttctacttc accgacacgt tctggccgga tttcgacgcc ggcgcgctcg ccgaagcgct cgcgtcgtac acgcaccgcg aacgccgctt cggccgcacg agcgcgcagc tcgaacccca ggcccaggac gccgattccc tttcatgcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a