BupsA.00871.a

Oxidoreductase, zinc-binding dehydrogenase family protein

CENTER ID: BupsA.00871.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00871.a.A1.GE32546 Full length( BupsA.00871.a ) 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00871.a.A1.PW32466 Full length( BupsA.00871.a ) 1 332

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2560
RefSeq: YP_333844.1
UniProt: Q3JRF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQVNRRVLL VSRPEREARV ENFELVETPL APLAEGEVRV RNHFLSIDPY MRGRMNAGRS YAEPQPLGEV MGGGTAGEVV ESRNPAFAPG DRVIGAYGWQ EYGTSTGKEL RKVDTTRVPL SAYLGAAGMP GVTAWYGLNR IIRPRAGETL VVSAASGAVG SVVGQLAKLA GCRAVGIAGG ADKCRYVVDT LGFDACVDYK AGRLADDLAA AAPDGVDGCF ENVGGAVLDA TLARMNPFGR IAMCGMIAAY DGAPAPLANP ALILRERLLV QGFIVSEHFD VWPEALAQLA SLVANRQLHY RETIAQGLER APDALLGLLK GRNFGKQLVA LV
NT Sequence
ATGTCACAGG TCAACCGGCG GGTGCTGCTC GTGTCGCGCC CCGAACGCGA GGCGCGCGTT GAGAACTTCG AACTCGTCGA GACGCCGCTT GCGCCGCTCG CCGAAGGCGA GGTGCGCGTG CGCAATCATT TTCTGTCGAT CGATCCGTAC ATGCGCGGGC GGATGAACGC GGGGCGCTCG TACGCCGAGC CGCAACCGCT CGGCGAGGTG ATGGGCGGCG GCACCGCCGG CGAGGTCGTC GAGTCGCGCA ATCCGGCGTT CGCCCCCGGC GATCGCGTGA TCGGCGCGTA CGGCTGGCAG GAGTACGGCA CGTCGACGGG CAAGGAACTG CGCAAGGTCG ACACGACGCG CGTGCCGCTG TCCGCGTATC TCGGAGCCGC CGGAATGCCC GGCGTGACCG CGTGGTACGG CCTGAACCGG ATCATCCGGC CGCGCGCGGG CGAGACGCTC GTCGTCAGCG CGGCGAGCGG CGCGGTCGGC AGCGTGGTCG GGCAGCTCGC GAAGCTCGCC GGGTGTCGCG CGGTCGGCAT CGCGGGCGGC GCGGACAAGT GCCGCTACGT CGTCGATACG CTCGGCTTCG ATGCGTGCGT CGACTACAAG GCGGGCCGGC TCGCCGACGA TCTCGCGGCC GCCGCGCCGG ACGGCGTCGA CGGCTGTTTC GAGAACGTCG GCGGCGCGGT GCTCGATGCG ACGCTCGCGC GGATGAACCC GTTCGGGCGC ATCGCGATGT GCGGGATGAT CGCCGCGTAC GACGGCGCGC CCGCGCCGCT CGCGAACCCG GCGCTGATCC TGCGCGAGCG GCTGCTCGTG CAGGGCTTCA TCGTGTCCGA GCACTTCGAC GTGTGGCCCG AGGCGCTCGC GCAGCTCGCG TCGCTCGTCG CGAACAGGCA GCTGCATTAT CGGGAGACGA TCGCGCAGGG CCTCGAGCGC GCGCCCGACG CGCTGCTCGG GCTGCTGAAA GGGCGCAATT TCGGCAAGCA GCTCGTCGCG CTCGTC
Details for BupsA.00871.a.A1.GE32546
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQVNRRVL LVSRPEREAR VENFELVETP LAPLAEGEVR VRNHFLSIDP YMRGRMNAGR SYAEPQPLGE VMGGGTAGEV VESRNPAFAP GDRVIGAYGW QEYGTSTGKE LRKVDTTRVP LSAYLGAAGM PGVTAWYGLN RIIRPRAGET LVVSAASGAV GSVVGQLAKL AGCRAVGIAG GADKCRYVVD TLGFDACVDY KAGRLADDLA AAAPDGVDGC FENVGGAVLD ATLARMNPFG RIAMCGMIAA YDGAPAPLAN PALILRERLL VQGFIVSEHF DVWPEALAQL ASLVANRQLH YRETIAQGLE RAPDALLGLL KGRNFGKQLV A
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcac aggtcaaccg gcgggtgctg ctcgtgtcgc gccccgaacg cgaggcgcgc gttgagaact tcgaactcgt cgagacgccg cttgcgccgc tcgccgaagg cgaggtgcgc gtgcgcaatc attttctgtc gatcgatccg tacatgcgcg ggcggatgaa cgcggggcgc tcgtacgccg agccgcaacc gctcggcgag gtgatgggcg gcggcaccgc cggcgaggtc gtcgagtcgc gcaatccggc gttcgccccc ggcgatcgcg tgatcggcgc gtacggctgg caggagtacg gcacgtcgac gggcaaggaa ctgcgcaagg tcgacacgac gcgcgtgccg ctgtccgcgt atctcggagc cgccggaatg cccggcgtga ccgcgtggta cggcctgaac cggatcatcc ggccgcgcgc gggcgagacg ctcgtcgtca gcgcggcgag cggcgcggtc ggcagcgtgg tcgggcagct cgcgaagctc gccgggtgtc gcgcggtcgg catcgcgggc ggcgcggaca agtgccgcta cgtcgtcgat acgctcggct tcgatgcgtg cgtcgactac aaggcgggcc ggctcgccga cgatctcgcg gccgccgcgc cggacggcgt cgacggctgt ttcgagaacg tcggcggcgc ggtgctcgat gcgacgctcg cgcggatgaa cccgttcggg cgcatcgcga tgtgcgggat gatcgccgcg tacgacggcg cgcccgcgcc gctcgcgaac ccggcgctga tcctgcgcga gcggctgctc gtgcagggct tcatcgtgtc cgagcacttc gacgtgtggc ccgaggcgct cgcgcagctc gcgtcgctcg tcgcgaacag gcagctgcat tatcgggaga cgatcgcgca gggcctcgag cgcgcgcccg acgcgctgct cgggctgctg aaagggcgca atttcggcaa gcagctcgtc gcgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQVNRRVL LVSRPEREAR VENFELVETP LAPLAEGEVR VRNHFLSIDP YMRGRMNAGR SYAEPQPLGE VMGGGTAGEV VESRNPAFAP GDRVIGAYGW QEYGTSTGKE LRKVDTTRVP LSAYLGAAGM PGVTAWYGLN RIIRPRAGET LVVSAASGAV GSVVGQLAKL AGCRAVGIAG GADKCRYVVD TLGFDACVDY KAGRLADDLA AAAPDGVDGC FENVGGAVLD ATLARMNPFG RIAMCGMIAA YDGAPAPLAN PALILRERLL VQGFIVSEHF DVWPEALAQL ASLVANRQLH YRETIAQGLE RAPDALLGLL KGRNFGKQLV ALV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcacaggtc aaccggcggg tgctgctcgt gtcgcgcccc gaacgcgagg cgcgcgttga gaacttcgaa ctcgtcgaga cgccgcttgc gccgctcgcc gaaggcgagg tgcgcgtgcg caatcatttt ctgtcgatcg atccgtacat gcgcgggcgg atgaacgcgg ggcgctcgta cgccgagccg caaccgctcg gcgaggtgat gggcggcggc accgccggcg aggtcgtcga gtcgcgcaat ccggcgttcg cccccggcga tcgcgtgatc ggcgcgtacg gctggcagga gtacggcacg tcgacgggca aggaactgcg caaggtcgac acgacgcgcg tgccgctgtc cgcgtatctc ggagccgccg gaatgcccgg cgtgaccgcg tggtacggcc tgaaccggat catccggccg cgcgcgggcg agacgctcgt cgtcagcgcg gcgagcggcg cggtcggcag cgtggtcggg cagctcgcga agctcgccgg gtgtcgcgcg gtcggcatcg cgggcggcgc ggacaagtgc cgctacgtcg tcgatacgct cggcttcgat gcgtgcgtcg actacaaggc gggccggctc gccgacgatc tcgcggccgc cgcgccggac ggcgtcgacg gctgtttcga gaacgtcggc ggcgcggtgc tcgatgcgac gctcgcgcgg atgaacccgt tcgggcgcat cgcgatgtgc gggatgatcg ccgcgtacga cggcgcgccc gcgccgctcg cgaacccggc gctgatcctg cgcgagcggc tgctcgtgca gggcttcatc gtgtccgagc acttcgacgt gtggcccgag gcgctcgcgc agctcgcgtc gctcgtcgcg aacaggcagc tgcattatcg ggagacgatc gcgcagggcc tcgagcgcgc gcccgacgcg ctgctcgggc tgctgaaagg gcgcaatttc ggcaagcagc tcgtcgcgct cgtctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.00871.a.A1.PW32466
PURIFICATION DATe: 7/8/2011
CONCENTRATION: 32.22mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQVNRRVL LVSRPEREAR VENFELVETP LAPLAEGEVR VRNHFLSIDP YMRGRMNAGR SYAEPQPLGE VMGGGTAGEV VESRNPAFAP GDRVIGAYGW QEYGTSTGKE LRKVDTTRVP LSAYLGAAGM PGVTAWYGLN RIIRPRAGET LVVSAASGAV GSVVGQLAKL AGCRAVGIAG GADKCRYVVD TLGFDACVDY KAGRLADDLA AAAPDGVDGC FENVGGAVLD ATLARMNPFG RIAMCGMIAA YDGAPAPLAN PALILRERLL VQGFIVSEHF DVWPEALAQL ASLVANRQLH YRETIAQGLE RAPDALLGLL KGRNFGKQLV A
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcac aggtcaaccg gcgggtgctg ctcgtgtcgc gccccgaacg cgaggcgcgc gttgagaact tcgaactcgt cgagacgccg cttgcgccgc tcgccgaagg cgaggtgcgc gtgcgcaatc attttctgtc gatcgatccg tacatgcgcg ggcggatgaa cgcggggcgc tcgtacgccg agccgcaacc gctcggcgag gtgatgggcg gcggcaccgc cggcgaggtc gtcgagtcgc gcaatccggc gttcgccccc ggcgatcgcg tgatcggcgc gtacggctgg caggagtacg gcacgtcgac gggcaaggaa ctgcgcaagg tcgacacgac gcgcgtgccg ctgtccgcgt atctcggagc cgccggaatg cccggcgtga ccgcgtggta cggcctgaac cggatcatcc ggccgcgcgc gggcgagacg ctcgtcgtca gcgcggcgag cggcgcggtc ggcagcgtgg tcgggcagct cgcgaagctc gccgggtgtc gcgcggtcgg catcgcgggc ggcgcggaca agtgccgcta cgtcgtcgat acgctcggct tcgatgcgtg cgtcgactac aaggcgggcc ggctcgccga cgatctcgcg gccgccgcgc cggacggcgt cgacggctgt ttcgagaacg tcggcggcgc ggtgctcgat gcgacgctcg cgcggatgaa cccgttcggg cgcatcgcga tgtgcgggat gatcgccgcg tacgacggcg cgcccgcgcc gctcgcgaac ccggcgctga tcctgcgcga gcggctgctc gtgcagggct tcatcgtgtc cgagcacttc gacgtgtggc ccgaggcgct cgcgcagctc gcgtcgctcg tcgcgaacag gcagctgcat tatcgggaga cgatcgcgca gggcctcgag cgcgcgcccg acgcgctgct cgggctgctg aaagggcgca atttcggcaa gcagctcgtc gcgc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQVNRRVL LVSRPEREAR VENFELVETP LAPLAEGEVR VRNHFLSIDP YMRGRMNAGR SYAEPQPLGE VMGGGTAGEV VESRNPAFAP GDRVIGAYGW QEYGTSTGKE LRKVDTTRVP LSAYLGAAGM PGVTAWYGLN RIIRPRAGET LVVSAASGAV GSVVGQLAKL AGCRAVGIAG GADKCRYVVD TLGFDACVDY KAGRLADDLA AAAPDGVDGC FENVGGAVLD ATLARMNPFG RIAMCGMIAA YDGAPAPLAN PALILRERLL VQGFIVSEHF DVWPEALAQL ASLVANRQLH YRETIAQGLE RAPDALLGLL KGRNFGKQLV ALV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCACAGGTC AACCGGCGGG TGCTGCTCGT GTCGCGCCCC GAACGCGAGG CGCGCGTTGA GAACTTCGAA CTCGTCGAGA CGCCGCTTGC GCCGCTCGCC GAAGGCGAGG TGCGCGTGCG CAATCATTTT CTGTCGATCG ATCCGTACAT GCGCGGGCGG ATGAACGCGG GGCGCTCGTA CGCCGAGCCG CAACCGCTCG GCGAGGTGAT GGGCGGCGGC ACCGCCGGCG AGGTCGTCGA GTCGCGCAAT CCGGCGTTCG CCCCCGGCGA TCGCGTGATC GGCGCGTACG GCTGGCAGGA GTACGGCACG TCGACGGGCA AGGAACTGCG CAAGGTCGAC ACGACGCGCG TGCCGCTGTC CGCGTATCTC GGAGCCGCCG GAATGCCCGG CGTGACCGCG TGGTACGGCC TGAACCGGAT CATCCGGCCG CGCGCGGGCG AGACGCTCGT CGTCAGCGCG GCGAGCGGCG CGGTCGGCAG CGTGGTCGGG CAGCTCGCGA AGCTCGCCGG GTGTCGCGCG GTCGGCATCG CGGGCGGCGC GGACAAGTGC CGCTACGTCG TCGATACGCT CGGCTTCGAT GCGTGCGTCG ACTACAAGGC GGGCCGGCTC GCCGACGATC TCGCGGCCGC CGCGCCGGAC GGCGTCGACG GCTGTTTCGA GAACGTCGGC GGCGCGGTGC TCGATGCGAC GCTCGCGCGG ATGAACCCGT TCGGGCGCAT CGCGATGTGC GGGATGATCG CCGCGTACGA CGGCGCGCCC GCGCCGCTCG CGAACCCGGC GCTGATCCTG CGCGAGCGGC TGCTCGTGCA GGGCTTCATC GTGTCCGAGC ACTTCGACGT GTGGCCCGAG GCGCTCGCGC AGCTCGCGTC GCTCGTCGCG AACAGGCAGC TGCATTATCG GGAGACGATC GCGCAGGGCC TCGAGCGCGC GCCCGACGCG CTGCTCGGGC TGCTGAAAGG GCGCAATTTC GGCAAGCAGC TCGTCGCGCT CGTCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA