BupsA.00863.g

Sensor protein (EC 2.7.13.3)

CENTER ID: BupsA.00863.g
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00863.g.A1.GE26325 periplasmic domain 42 189
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_131510
PATRIC ID: fig|320372.6.peg.4379
RefSeq: YP_335541.1
UniProt: Q3JLL2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASGIGRFGK RASRLIDFGL WAAAALLLLT AGVGALGTAR LSDNEADIHR ASEVISGLER LLALARDMET SQRGYILTGK TDYLQPYKAA LPEIRKQFDA LATLVQGDAV QQQRLATLRM LLEKKQHELA ATIELRKTEG FDAAQAIVSS DAGKIFMDRA RVMVNEMEAT VQQQLRTLRD RARATRDIAI GVGLASGLLT LAVCVSFGYV MRRLLLVEAA AAHDLFEQRE LLHVTLASIG DGVIATDVGG RITFFNRVAE EMTALCADAV VGQPIEQVLT FRHAATQRVI DNPARVAVER RRTASLPAHT RFVGRSGGEL PVDGNAAPTF DANGKLVGAV LVVRDVSERE RAEERFRLAV EAAPTAMIMV GRDGRIVLVN SQAEKLFGYA RGDMIGHGID MLVPERFRAG HWAHRDAFFG NPDARPMGEG RDLFGLRRDG SEFPVEIGLN PITTSEGAFV LCSIADITAR KRAELELRRR SEELARSNQD LEQFAYVASH DLQEPLRAVA GPLQLLQRRY QGQLDARADE FIGHAVDGAT RMQSLIDDLL SYSRVGRLED ARRPVDCEHV LDEALRNLSA AIRESGAQVT HDPLPTVHGI EAQLVLLFQN LIGNAIKFRG KDRVARIHLE AHTRDDAWLL RVRDNGIGID PQYFERIFLI FQRLHTRREY PGTGLGLALC KRIVEHHGGR IFVESEPGQG TTFSFTLSRP GADETRWPRD PRE
NT Sequence
atggcgtcgg gcatcgggcg attcgggaaa agagcgtcgc gcttgatcga tttcggtttg tgggccgccg ccgcgctgtt actgctgacg gcgggcgtcg gcgcgctcgg cacggcccgc ctgtccgaca acgaggcgga tatccatcgc gcgagcgagg tgatatcggg tctcgaacgg ctgctggcgc tggcccgcga catggagacg agccagcgcg gatatatcct caccggcaag acggactacc ttcagcccta caaggcggca ctgcccgaaa tccgcaagca gttcgacgcg ctcgcgacgc ttgtgcaggg cgatgccgtt cagcagcagc ggctcgcgac gctgcgcatg ctgctcgaga aaaagcagca cgagctcgcg gcgacgatcg agctgcgcaa gacggagggg ttcgacgcgg cgcaggcgat cgtctcgagc gatgcgggca agatcttcat ggaccgcgcg cgcgtgatgg tcaacgaaat ggaggcgacc gtccagcagc aactgaggac gctgcgcgat cgcgccaggg cgacacgcga catcgcgatc ggcgtggggc tcgcgtccgg gctgctcacg ctcgccgtgt gcgtatcgtt cggctatgtg atgcgccgcc tgctgctcgt cgaggcggcc gcggcccacg atctgttcga gcagcgcgaa ctgctgcacg taacgctcgc gagcatcggc gacggcgtga tcgcgacgga cgtcggcggc cgcatcacgt tcttcaaccg ggtcgccgag gagatgacgg cgctttgcgc cgatgccgtc gtcggtcagc cgatcgagca ggtgctgact tttcggcatg ccgcgacgca gcgggtgatc gacaatccgg cgcgcgtcgc ggtcgagcgc aggcgcaccg cgagcttgcc tgcccatacg cgcttcgtcg gccgcagcgg cggggaattg ccggtggacg gcaacgccgc accgacgttc gacgcgaacg gcaagctcgt cggcgcggtg ctcgtcgtgc gcgacgtgtc ggagcgcgag cgggccgagg agcgattccg gctcgccgtc gaggccgcgc cgaccgcgat gatcatggtg gggcgcgacg gcaggatcgt gctcgtcaat tcgcaggccg agaaattgtt cgggtacgcg cgcggcgaca tgatcgggca cggcatcgac atgctggtgc ccgagcgttt tcgtgcgggg cattgggcgc accgcgacgc gttcttcggc aacccggacg cgcggccgat gggcgagggg cgggacctgt tcggtttgcg ccgcgacggc agcgagtttc cggtcgagat cggcctgaat ccgatcacga cctcggaggg cgcgttcgtg ctgtgctcga tcgccgacat caccgcgcgc aagcgcgcgg aactcgaact gcgccgcaga agcgaggagc ttgcgcgctc gaatcaggat ctcgagcagt tcgcctacgt cgcgtctcac gatctgcagg agccgttgcg cgcggtcgcg gggcccctgc aattgctgca gcggcgatac caggggcagc tcgacgcgcg ggccgacgag ttcatcgggc acgcggtgga tggcgcgacg agaatgcagt cgctgatcga cgacctgctc agctattcgc gcgtcgggcg gctcgaggac gcgcgccggc cggtcgactg cgagcacgtg ctggacgagg cgctgcgcaa cctgagcgcg gcgattcgcg aatccggcgc gcaggtcacg cacgatccgc tgccgaccgt gcatggcatc gaggcacagc tcgtgctgct gttccagaat ctgatcggca acgcgatcaa gtttcgcggc aaggaccgcg tcgcgcggat tcatctggaa gcgcacacgc gcgacgacgc atggctgttg cgcgtgcgcg acaacggcat cgggatcgat ccccagtatt tcgagcgcat cttcctgatt ttccagcggc tgcacacgcg gcgcgaatat ccgggaacgg ggctcgggct cgcgctgtgc aagcggatcg tcgagcacca tggcggccgc atcttcgtcg agtccgagcc ggggcagggc acgacgtttt catttacgct gtccaggccg ggtgccgatg aaacgcgttg gccgcgcgat ccgagggagt ga
Details for BupsA.00863.g.A1.GE26325
HARVESTED ON: 3/10/2009
SEQUENCED ON: 3/26/2009
EXPECTED MW: 19kDa
OBSERVED MW: 19kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDNEADIH RASEVISGLE RLLALARDME TSQRGYILTG KTDYLQPYKA ALPEIRKQFD ALATLVQGDA VQQQRLATLR MLLEKKQHEL AATIELRKTE GFDAAQAIVS SDAGKIFMDR ARVMVNEMEA TVQQQLRTLR DRARATRDIA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtccg acaacgaggc ggatatccat cgcgcgagcg aggtgatatc gggtctcgaa cggctgctgg cgctggcccg cgacatggag acgagccagc gcggatatat cctcaccggc aagacggact accttcagcc ctacaaggcg gcactgcccg aaatccgcaa gcagttcgac gcgctcgcga cgcttgtgca gggcgatgcc gttcagcagc agcggctcgc gacgctgcgc atgctgctcg agaaaaagca acacgagctc gcggcgacga tcgagctgcg caagacggag gggttcgacg cggcgcaggc gatcgtctcg agcgatgcgg gcaagatctt catggaccgc gcgcgcgtga tggtcaacga aatggaggcg accgtccagc agcaactgag gacgctgcgc gatcgcgcca gggcgacacg cgacatcgcg taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDNEADIH RASEVISGLE RLLALARDME TSQRGYILTG KTDYLQPYKA ALPEIRKQFD ALATLVQGDA VQQQRLATLR MLLEKKQHEL AATIELRKTE GFDAAQAIVS SDAGKIFMDR ARVMVNEMEA TVQQQLRTLR DRARATRDIA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgacaac gaggcggata tccatcgcgc gagcgaggtg atatcgggtc tcgaacggct gctggcgctg gcccgcgaca tggagacgag ccagcgcgga tatatcctca ccggcaagac ggactacctt cagccctaca aggcggcact gcccgaaatc cgcaagcagt tcgacgcgct cgcgacgctt gtgcagggcg atgccgttca gcagcagcgg ctcgcgacgc tgcgcatgct gctcgagaaa aagcagcacg agctcgcggc gacgatcgag ctgcgcaaga cggaggggtt cgacgcggcg caggcgatcg tctcgagcga tgcgggcaag atcttcatgg accgcgcgcg cgtgatggtc aacgaaatgg aggcgaccgt ccagcagcaa ctgaggacgc tgcgcgatcg cgccagggcg acacgcgaca tcgcgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa