BupsA.00863.f

Sensor protein (EC 2.7.13.3)

CENTER ID: BupsA.00863.f
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00863.f.A1.GE26326 periplasmic domain 286 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_147337
PATRIC ID: fig|320372.6.peg.3980
RefSeq: YP_335188.1
UniProt: Q3JML5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAREARARGR AAPAARIIEV NPDANTDRGR RAEDRHVSAQ GADRSGLHRG LGRGRRDGPA SGRDRGVRPD HPRRDAARPR RLDGARAAAP RALDARAVPD RARRRRRPRE GARARRGRLR RQAVRFRRAG RARALDPAPR AGARIDGPAD RRSGARPDAA QGHAPGRRRA ADREGIRAAV AADAPRGRDP AARDDRLAGV GHEFQQRHER RRRGDPPAAL EDRRRVRAEA DPHGARDGLR ARSQKRERAE PMIRRLLPRT LRARLTALII LSTAATLALS GVALYSALHN RLVGMSSYEM SATLAAMRTH LANVANVDDI PRKSDLWIDQ LHGHQNLDLA IYDTDGRLRF ATRGFVAPRP ALGAPQTRVP ASAAPAGATF SYLADDAPLR GGNPRTARIV VQYDGKNDHA LLRAYAYTVV VIEVLAVVLT AALAYGIAML GLSPLRRLVA RAEQMSSSRL AQPLPELDTS GELKEMEHAF NAMLKRLDES FVRLSQFSSN LAHDMRTPLT NLLAEAQVAL SKPRTADEYR DVIESSIDEY QRLSRMIEDM LFLARSDNAQ SHLAIRTLDA AAQAERVAGY YEPMAEDAEV SIVVRGKAEV RADALLYHRA LSNLISNALN HAPRGSTITI ECAQAADAAT ISVSDTGRGI EAPHRERIFE RFYRVDPARH NSASGTGLGL AIVRSIMENH GGTCGVDSEP HVRTTFWLKF PAHAA
NT Sequence
atggctcgcg aggctcgcgc ccgcggccgt gctgcgccgg cggcgcgaat catcgaggtg aatccagatg cgaatactga tcgtggaaga cgagccgaag accggcatgt atctgcgcaa ggggctgacc gaagcgggct tcatcgcgga ctgggtcgag gacggcgtga cgggcctgca tcaggccgag accgaggagt acgacctgat catcctcgac gtgatgctgc ccggccacga cggctggacg gtgctcgagc ggctgcgccg cgcgcactcg acgcccgtgc tgttcctgac cgcgcgcgac gacgtcggcg accgcgtgaa ggggctcgag ctcggcgcgg acgattacgt cgtcaagccg ttcgatttcg tcgagctggt cgcgcgcgtg cgctcgatcc tgcgccgcgg gcaggcgcgc gaatcgacgg tcctgcggat cgccgatctg gagctcgacc tgacgcggcg caaggccacg cgccagggcg acgtcgtgct gctgaccgcg aaggaattcg cgctgctgtg gctgctgatg cgccgcgagg gcgagatcct gccgcgcgcg acgatcgcct cgcaggtgtg ggacatgaat ttcaacagcg acacgaacgt cgtcgacgcg gcgatccgcc ggctgcgctc gaagatcgac gacgcgtacg agccgaagct gatccacacg gtgcgcggga tgggctacgt gctcgaagtc agaagcgcga gcgcgccgag ccgatgatca gacgcctgct gccccgcacg ctgcgcgcgc gcctgacggc gctcatcatc ctgtcgaccg cggcgacgct cgcgctgagc ggcgtcgcgc tctacagcgc gctgcacaac cggctcgtcg gcatgtcgtc gtacgagatg tcggcgacgc tcgcggcgat gcgcacgcat ctcgcgaacg tcgcgaacgt cgacgatatc ccgcgcaaat cggatctgtg gatcgatcaa ctgcacggcc atcagaatct ggatctcgcg atctacgaca ccgacggccg cctgcgcttc gccacgcgcg gcttcgtcgc gccgcggccc gcgctcggcg cgccgcaaac gcgcgtgccg gcgagcgccg cgcccgccgg cgcgacgttc agctatctcg cggacgacgc gccgctgcgc ggcgggaacc cgcgcaccgc gcgcatcgtc gtgcagtacg acggcaagaa cgatcatgcg ctgctgcgcg catacgcgta cacggtcgtc gtgatcgagg tgctcgcggt cgtgctgacg gcggcgctcg cctacggcat cgcgatgctc ggcctgagcc cgctgcggcg gctcgtcgcg cgcgccgagc agatgtcgtc gagccggctc gcgcagccgc tgccggagct cgacacgtcg ggcgagctca aggaaatgga gcacgcgttc aacgccatgc tcaagcgcct cgacgaatcg ttcgtccggc tgagccagtt ctcgtcgaat ctcgcgcacg acatgcgcac gccgctcacg aacctgctcg ccgaggcgca ggtcgcgcta tcgaagccgc gcacggccga cgaatatcgc gacgtgatcg aatcgagcat cgacgagtat cagcgtctgt cgcgcatgat cgaggacatg ctgttcctcg cgcgctccga caacgcgcag agccacctgg cgatccgcac gctcgacgcg gcggcgcaag ccgagcgcgt cgccggctat tacgagccga tggccgagga tgccgaagtg agcatcgtcg tgcgcggcaa ggccgaggtt cgggcggacg cgctgctcta tcaccgcgcg ctcagcaacc tgatcagcaa cgcgctcaat cacgcgccgc gcggctcgac gatcacgatc gaatgcgcgc aagccgccga cgcggcgacg atcagcgtat cggacacggg ccgcggcatc gaggcgccgc accgcgaacg gatcttcgag cggttctacc gcgtcgatcc ggcgcggcac aattcggcgt ccggcacggg gctcggcctg gcgatcgtgc gctcgatcat ggaaaaccac ggcggcacgt gcggcgtcga cagcgagccg cacgtgcgca cgacgttctg gctgaagttt cccgcgcacg cggcctga
Details for BupsA.00863.f.A1.GE26326
HARVESTED ON: 3/10/2009
SEQUENCED ON: 3/26/2009
EXPECTED MW: 16kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSALHNRLV GMSSYEMSAT LAAMRTHLAN VANVDDIPRK SDLWIDQLHG HQNLDLAIYD TDGRLRFATR GFVAPRPALG APQTRVPASA APAGATFSYL ADDAPLRGGN PRTARIVVQY DGKNDHALLR A
Validated NT Sequence
ttattatgcg cgcagcagcg catgatcgtt cttgccgtcg tactgcacga cgatgcgcgc ggtgcgcggg ttcccgccgc gcagcggcgc gtcgtccgcg agatagctga acgtcgcgcc ggcgggcgcg gcgctcgccg gcacgcgcgt ttgcggcgcg ccgagcgcgg gccgcggcgc gacgaagccg cgcgtggcga agcgcaggcg gccgtcggtg tcgtagatcg cgagatccag attctgatgg ccgtgcagtt gatcgatcca cagatccgat ttgcgcggga tatcgtcgac gttcgcgacg ttcgcgagat gcgtgcgcat cgccgcgagc gtcgccgaca tctcgtacga cgacatgccg acgagccggt tgtgcagcgc gctcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtnnnnctcc ttcttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSALHNRLV GMSSYEMSAT LAAMRTHLAN VANVDDIPRK SDLWIDQLHG HQNLDLAIYD TDGRLRFATR GFVAPRPALG APQTRVPASA APAGATFSYL ADDAPLRGGN PRTARIVVQY DGKNDHALLR A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgctg cacaaccggc tcgtcggcat gtcgtcgtac gagatgtcgg cgacgctcgc ggcgatgcgc acgcatctcg cgaacgtcgc gaacgtcgac gatatcccgc gcaaatcgga tctgtggatc gatcaactgc acggccatca gaatctggat ctcgcgatct acgacaccga cggccgcctg cgcttcgcca cgcgcggctt cgtcgcgccg cggcccgcgc tcggcgcgcc gcaaacgcgc gtgccggcga gcgccgcgcc cgccggcgcg acgttcagct atctcgcgga cgacgcgccg ctgcgcggcg ggaacccgcg caccgcgcgc atcgtcgtgc agtacgacgg caagaacgat catgcgctgc tgcgcgcaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa