BupsA.00863.e

Sensor protein (EC 2.7.13.3)

CENTER ID:	BupsA.00863.e
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00863.e.A1.GE26313	periplasmic domain		35	182

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00863.e.A1.PW26360	periplasmic domain		35	182

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130368
PATRIC ID:	fig\|320372.6.peg.963
RefSeq:	YP_332368.1
UniProt:	Q3JVN5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRVEWRNEK IIVALGSLWI LGFAAWAFLL YDLLGTSVKE GILEGPREGV FWTAAQYRNS FSRFDRQLIL YAAGENRDFD AVLLQLDSLE ASFGFLERPS EVSAYWLSIP KARGDIAELS RFMASLRRDV PALRARPDDT KRVLGELARQ WPKVNALANY FRAIEMEQRD FTFHQLKEKR RAIVMLGGVL GIILGALFLL LFYTVRTRGS LLEQQQAALD AERKASDRAF EMIAAKNAFL GMVSHELRTP LQAICGSIEV LLARPQSEAN MKTIKRLQNS AASLEAQVKD LTDYIKLRST NRSVQSEIVE VAPLLADVLD PLRGRIRDKH LSTSLRVEPP ALVVRSDRKL IQQIVSNLVE NAIKYTNSGT IEISAALGGT PANRTMAITV RDTGVGIARH LLAKIFEPFF RVNDPGVRHV DGIGMGLAVV QELVVALRGH VDVRSAVGEG SEFAVTLPVE LPGRADALDD DAQPSPRAAH RDLRALVVDD NENARETLGA MLATLGIRVD LRGTGKEGLR CFGECQHDIV VLDLELPDIS GFEVAEQIRW ATSSDAARKT TILGVSAYES ALLKGDHAIF DAFIPKPIHL DTLGGIVSRL RS

NT Sequence

atgagccggg tcgaatggcg caatgaaaaa atcatcgtcg ccctgggttc cctgtggatc ctgggcttcg ccgcgtgggc gttcctgctg tacgacctgc tcggcacgtc cgtcaaggaa ggcattctgg aaggcccgcg cgaaggcgtg ttctggactg ccgcgcaata ccggaactcg ttcagccggt tcgatcggca actgattctc tacgcggccg gcgagaaccg cgacttcgac gccgtgctgc tccagctcga cagcctcgaa gcgtcgttcg gctttctcga gcggccgtcc gaagtctccg cgtactggct gagcattccg aaggcccgtg gcgacatcgc cgagctgtcg cgcttcatgg cgagcctgcg ccgcgacgtt ccggcgctgc gcgcgcgccc cgacgatacg aagcgcgtgc tgggcgagct cgcccggcaa tggcccaagg tgaacgcgct cgcgaattac tttcgcgcga tcgaaatgga gcagcgcgat ttcacgttcc atcagttgaa ggagaagcgg cgcgcgatcg tcatgctggg cggcgtgctc ggcatcattc tcggcgcgct gttcctgctg ctgttctaca cggtccgcac gcgcggcagc ctgctcgagc agcagcaggc ggcgctcgac gcggaacgca aggcgtccga tcgcgcgttc gagatgatcg ccgcgaaaaa cgcgttcctc ggcatggtga gccatgagct gcgcacgccg ctgcaggcga tctgcggctc gatcgaagtg ctgctcgcgc ggccgcagtc cgaagcgaac atgaagacga tcaagcggct gcagaactcg gccgcgtcgc tcgaagcgca ggtgaaggac ctgaccgact acatcaagct gcggtccacg aatcgatcgg tgcagtcgga gatcgtcgag gtcgcgccgc tgctcgccga cgtgctcgat ccgttgcgcg gccgcatccg cgacaagcat ctgagcacgt cgctgcgcgt ggagccgccc gcgctcgtcg tccggtccga tcgcaagctg atccagcaga tcgtgtcgaa cctcgtcgag aacgcgatca aatacacgaa cagcggaacg atcgagatct cggccgcgct cggcggcacg cccgccaatc ggaccatggc gatcacggtg cgcgacaccg gcgtcggcat cgcgcggcat ctgctcgcga agatcttcga gccgttcttt cgcgtgaacg atccgggcgt gcggcacgtc gacggcatcg gcatggggct cgcggtcgtc caggagctcg tcgtcgcgct gcgcgggcac gtcgatgtgc gcagcgccgt cggcgaaggc agcgaattcg ccgtcacgct gcccgtcgag ctgcccggcc gcgccgatgc gctcgacgac gacgcgcagc cgtcaccgcg ggcggcgcat cgcgatctgc gcgcgctcgt cgtcgacgac aacgagaacg cgcgcgaaac gctgggcgcg atgctcgcga cgctcggcat ccgggtggat ctgcgcggca ccggcaagga aggcttgcgc tgcttcggcg aatgtcagca cgacatcgtc gtgctggatc tggagttgcc cgacatcagc ggtttcgagg tggccgagca gatacgctgg gcgacgtcgt ccgacgcggc caggaagacg acgatactcg gcgtgagcgc gtacgaatcg gcgctgctca agggcgatca cgcgatcttc gacgccttca tccccaagcc gattcatctg gacacgctcg gcggcatcgt gagccggctg cgaagctga

Details for BupsA.00863.e.A1.GE26313

HARVESTED ON:	3/10/2009
SEQUENCED ON:	3/26/2009
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGTSVKEGI LEGPREGVFW TAAQYRNSFS RFDRQLILYA AGENRDFDAV LLQLDSLEAS FGFLERPSEV SAYWLSIPKA RGDIAELSRF MASLRRDVPA LRARPDDTKR VLGELARQWP KVNALANYFR AIEMEQRDFT FHQLKEKRRA

Validated NT Sequence

ttattacgcg cgccgcttct ccttcaactg atggaacgtg aaatcgcgct gctccatttc gatcgcgcga aagtaattcg cgagcgcgtt caccttgggc cattgccggg cgagctcgcc cagcacgcgc ttcgtatcgt cggggcgcgc gcgcagcgcc ggaacgtcgc ggcgcaggct cgccatgaag cgcgacagct cggcgatgtc gccacgggcc ttcggaatgc tcagccagta cgcggagact tcggacggcc gctcgagaaa gccgaacgac gcttcgaggc tgtcgagctg gagcagcacg gcgtcgaagt cgcggttctc gccggccgcg tagagaatca gttgccgatc gaaccggctg aacgagttcc ggtattgcgc ggcagtccag aacacgcctt cgcgcgggcc ttccagaatg ccttccttga cggacgtgcc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGTSVKEGI LEGPREGVFW TAAQYRNSFS RFDRQLILYA AGENRDFDAV LLQLDSLEAS FGFLERPSEV SAYWLSIPKA RGDIAELSRF MASLRRDVPA LRARPDDTKR VLGELARQWP KVNALANYFR AIEMEQRDFT FHQLKEKRRA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcacgtcc gtcaaggaag gcattctgga aggcccgcgc gaaggcgtgt tctggactgc cgcgcaatac cggaactcgt tcagccggtt cgatcggcaa ctgattctct acgcggccgg cgagaaccgc gacttcgacg ccgtgctgct ccagctcgac agcctcgaag cgtcgttcgg ctttctcgag cggccgtccg aagtctccgc gtactggctg agcattccga aggcccgtgg cgacatcgcc gagctgtcgc gcttcatggc gagcctgcgc cgcgacgttc cggcgctgcg cgcgcgcccc gacgatacga agcgcgtgct gggcgagctc gcccggcaat ggcccaaggt gaacgcgctc gcgaattact ttcgcgcgat cgaaatggag cagcgcgatt tcacgttcca tcagttgaag gagaagcggc gcgcgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BupsA.00863.e.A1.PW26360

PURIFICATION DATe:	4/14/2009
CONCENTRATION:	4.3mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	22
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGTSVKEGI LEGPREGVFW TAAQYRNSFS RFDRQLILYA AGENRDFDAV LLQLDSLEAS FGFLERPSEV SAYWLSIPKA RGDIAELSRF MASLRRDVPA LRARPDDTKR VLGELARQWP KVNALANYFR AIEMEQRDFT FHQLKEKRRA

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGTSVKEGI LEGPREGVFW TAAQYRNSFS RFDRQLILYA AGENRDFDAV LLQLDSLEAS FGFLERPSEV SAYWLSIPKA RGDIAELSRF MASLRRDVPA LRARPDDTKR VLGELARQWP KVNALANYFR AIEMEQRDFT FHQLKEKRRA

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCACGTCC GTCAAGGAAG GCATTCTGGA AGGCCCGCGC GAAGGCGTGT TCTGGACTGC CGCGCAATAC CGGAACTCGT TCAGCCGGTT CGATCGGCAA CTGATTCTCT ACGCGGCCGG CGAGAACCGC GACTTCGACG CCGTGCTGCT CCAGCTCGAC AGCCTCGAAG CGTCGTTCGG CTTTCTCGAG CGGCCGTCCG AAGTCTCCGC GTACTGGCTG AGCATTCCGA AGGCCCGTGG CGACATCGCC GAGCTGTCGC GCTTCATGGC GAGCCTGCGC CGCGACGTTC CGGCGCTGCG CGCGCGCCCC GACGATACGA AGCGCGTGCT GGGCGAGCTC GCCCGGCAAT GGCCCAAGGT GAACGCGCTC GCGAATTACT TTCGCGCGAT CGAAATGGAG CAGCGCGATT TCACGTTCCA TCAGTTGAAG GAGAAGCGGC GCGCGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA