BupsA.00863.d

Sensor protein (EC 2.7.13.3)

CENTER ID:	BupsA.00863.d
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00863.d.A1.GE34749	periplasmic domain		80	341

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_155569
PATRIC ID:	fig\|320372.6.peg.656
RefSeq:	YP_332055.1
UniProt:	Q3JWJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAETAGRAA APAPGGAGPD ATRRAWPGPA AFSGGGYATI DDPYSPEAVT VKRRLLVVLA LAAMLAAACA LTWTLTWQSG IADLRDSAAA RVDRTTNALK STLDRYESLP YLLGGHPFVQ DVLADPANRR QVNRANAYLE DMNRHAHATA TYVITASGLC VAASNWRGPD SFTGIGYQFR PYFIDAVKGG TGRFFGIGTI SRDPGYYISQ PVRRDGKIIG VAVVKLNLEW FQGADASEPL VVADDHGVIF LSSVPQWKYH TLKPLPGPIA TSIHETRQYA HYPVTPLPLR VERVLGPDAQ IVRMGAARRA PRYLATRRLL GEPDWQLVTL APIEPVDADA RNATIVTAFG FVSLSLLAFY WRTRRARVKE MLRSRVLLQS AYAELNRRVE ERTADLSQAN ARLKKEVGER IRAEQELRAA HDELVQASKL AALGQMAAGI THELNQPLAA LRSFSDNTRV LLDRGEQAAA RENLEAIAAL TERMGKITNQ LKLFVGRAKP RNERAHVARA LRNVLALLKE RMKDVELVIT LRDETRDAAV AARFDPSRDE PALIARCEDL RLEQVLINLL GNALDAVAAV DAPRIDVAID ATAATLAITV RDNGPGIAPE SLARLFEPFF TTKEMGRGLG LGLAISSSIA RDAGGSLTAR NAPAGGAEFV LTLRRARTHH PDTSAAS

NT Sequence

atgaacgcgg aaacggcggg gcgcgcagcg gcgcccgcgc cgggcggggc cgggccggac gcgacgagac gcgcctggcc cggccccgcg gccttttcgg gcggcggcta tgccacaata gacgatcctt acagtccgga agccgtcacc gtgaagcgcc gcctgctcgt cgttctcgcg ctcgccgcga tgctcgcggc cgcgtgcgcg cttacctgga cgctcacgtg gcaaagcggc atcgccgatc tgcgcgacag cgcggccgcg cgcgtcgacc gcacgacgaa cgcgctgaag agcacgctcg accgttacga atcgctgccg tacctgctgg gcggccaccc gttcgtgcag gatgtcctcg ccgatccggc caaccgtcgc caggtgaacc gcgcgaatgc ctatctcgag gacatgaacc gccacgcgca cgcgacggcg acctacgtga tcaccgcgag cgggctgtgc gtcgccgcga gcaactggcg cggcccggac agcttcaccg gcatcggcta tcagtttcgg ccgtacttca tcgacgcggt gaagggcggc accggccgct tcttcgggat cggcacgatc tcgcgcgatc ccggctacta catctcgcag ccggtgcggc gcgacggcaa gatcatcggc gtcgccgtcg tcaagctcaa tctcgaatgg ttccagggcg cggacgcgtc ggagccgctc gtcgtcgccg acgaccacgg cgtgatcttc ctgtcgtcgg tgccgcagtg gaagtaccac acgctcaagc cgctgccggg gccgatcgcg acgtcgatcc acgagacgcg ccagtacgcg cactatccgg tgacgccgct gccgctgcgc gtcgagcgcg tgctcgggcc cgacgcgcag atcgtgcgga tgggcgccgc gcgccgcgcg ccgcgctatc tcgcgacgcg ccgcctgctc ggcgagcccg actggcagct cgtcacgctc gcgccgatcg agccggtcga cgccgacgcg cgcaacgcga cgatcgtcac cgcgttcggc ttcgtgtcgc tcagcctgct cgcgttctac tggcgcacgc gccgcgcgcg cgtgaaggag atgctgcgca gccgcgtgct gctgcaaagc gcgtatgcgg agctgaaccg gcgtgtcgag gagcgcacgg cggatctgtc gcaggcgaac gcgcggctca agaaggaagt cggcgagcgc atccgcgcgg agcaggaact gcgcgccgcg cacgacgagc tcgtccaggc gagcaagctc gccgcgctcg gccagatggc ggccggcatc acgcacgagc tgaaccagcc gctcgccgcg ctgcgcagct tctccgacaa cacccgcgtg ctgctcgacc gcggcgagca ggcggccgcg cgcgagaacc tggaggcgat cgccgcgctc accgagcgga tgggcaagat cacgaatcag ctcaagctgt tcgtcgggcg tgcgaagccg cgcaacgagc gggcgcacgt cgcgcgcgcg ctgcgcaacg tgctcgcgct gctcaaggaa cggatgaagg acgtcgagct ggtgatcacg ctgcgcgacg agacgcgcga tgccgccgtc gccgcgcgct tcgatccgtc acgggacgag ccggcgctca ttgcgcgctg cgaggatctg cggctcgagc aggtgctcat caacctgctc ggcaacgcgc tcgacgcggt cgcggcggtc gacgcgccgc gcatcgacgt ggcgatcgat gcgaccgccg cgacgctggc gatcaccgtg cgcgacaatg ggccgggcat cgcgccggaa tcgctcgcgc ggctgttcga gccgttcttc acgacgaagg aaatggggcg cggcctcggg ctcggcctcg cgatctcgtc gtcgatcgcg cgcgacgcgg gcggctcgct caccgcgcgc aacgcgccgg cgggcggcgc ggagttcgtc ttgacgctgc gccgcgcgcg cacgcatcat ccggacactt ccgccgcgag ttga

Details for BupsA.00863.d.A1.GE34749

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGIADLRDS AAARVDRTTN ALKSTLDRYE SLPYLLGGHP FVQDVLADPA NRRQVNRANA YLEDMNRHAH ATATYVITAS GLCVAASNWR GPDSFTGIGY QFRPYFIDAV KGGTGRFFGI GTISRDPGYY ISQPVRRDGK IIGVAVVKLN LEWFQGADAS EPLVVADDHG VIFLSSVPQW KYHTLKPLPG PIATSIHETR QYAHYPVTPL PLRVERVLGP XAXIVRMGAA RRAPRYLATR XLLXXXXWXL VXLAPIEPVD AXXR

Validated NT Sequence

annnnnnttn nnnnnnttaa gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg ggcatcgccg atctgcgcga cagcgcggcc gcgcgcgtcg accgcacgac gaacgcgctg aagagcacgc tcgaccgtta cgaatcgctg ccgtacctgc tgggcggcca cccgttcgtg caggatgtcc tcgccgatcc ggccaaccgt cgccaggtga accgcgcgaa tgcctatctc gaggacatga accgccacgc gcacgcgacg gcgacctacg tgatcaccgc gagcgggctg tgcgtcgccg cgagcaactg gcgcggcccg gacagcttca ccggcatcgg ctatcagttt cggccgtact tcatcgacgc ggtgaagggc ggcaccggcc gcttcttcgg gatcggcacg atctcgcgcg atcccggcta ctacatctcg cagccggtgc ggcgcgacgg caagatcatc ggcgtcgccg tcgtcaagct caatctcgaa tggttccagg gcgcggacgc gtcggagccg ctcgtcgtcg ccgacgacca cggcgtgatc ttcctgtcgt cggtgccgca gtggaagtac cacacgctca agccgctgcc ggggccgatc gcgacgtcna tccacgagac gcgccagtac gcgcactatc cggtgacgcc gctgccgctg cgcgtcgagc gcgtgctcgg gcccnacgcg canatcgtgc ggatgggcgc cgcgcgccgc gcgccgcgct atctcgcgac gcgccncctg ctcngcnann ccnantggna nctcgtcncg ctngcnccga tcgagccggt cgacgccnan ncgcgctaat aacagcacga acnagttctg canccnanct tctcgaggtg cnnnnnnnnc cccnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcatcgcc gatctgcgcg acagcgcggc cgcgcgcgtc gaccgcacga cgaacgcgct gaagagcacg ctcgaccgtt acgaatcgct gccgtacctg ctgggcggcc acccgttcgt gcaggatgtc ctcgccgatc cggccaaccg tcgccaggtg aaccgcgcga atgcctatct cgaggacatg aaccgccacg cgcacgcgac ggcgacctac gtgatcaccg cgagcgggct gtgcgtcgcc gcgagcaact ggcgcggccc ggacagcttc accggcatcg gctatcagtt tcggccgtac ttcatcgacg cggtgaaggg cggcaccggc cgcttcttcg ggatcggcac gatctcgcgc gatcccggct actacatctc gcagccggtg cggcgcgacg gcaagatcat cggcgtcgcc gtcgtcaagc tcaatctcga atggttccag ggcgcggacg cgtcggagcc gctcgtcgtc gccgacgacc acggcgtgat cttcctgtcg tcggtgccgc agtggaagta ccacacgctc aagccgctgc cggggccgat cgcgacgtcg atccacgaga cgcgccagta cgcgcactat ccggtgacgc cgctgccgct gcgcgtcgag cgcgtgctcg ggcccgacgc gcagatcgtg cggatgggcg ccgcgcgccg cgcgccgcgc tatctcgcga cgcgccgcct gctcggcgag cccgactggc agctcgtcac gctcgcgccg atcgagccgg tcgacgccga cgcgcgcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa