BupsA.00810.a

3-octaprenyl-4-hydroxybenzoate carboxy-lyase

CENTER ID: BupsA.00810.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00810.a.A1.GE30918 Full length( BupsA.00810.a ) 1 323
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3749
RefSeq: YP_334970.2
UniProt: Q3JN83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSSIISFLT PSYSKVSHGH PTTHQANRRR KPGRALHERH RAIPDVRLFG PRRPGAEGVR RRPVQDGQRP RRRRDPPGHQ GVLELADDPA ALREGRIRRR LGHHDGDVSV GRTAATVHRL IFRPAMEPRP APPRRLIVAI TGATGAIYGV RMLDMLRASG GVETHLLISS AGWLNIRHEL QLSKDDVLAR ADVVHSVRDV GASIASGSFA TEGMIVAPCS MKTLASIAHG LSDNLITRAA DVALKERRRL VLLVREAPFN LAHLRNMTAV TEMGGVIFPP VPAFYAMPKT IDEIVDHTVA RVLDMFALGA PLTAPWQGLR EHG
NT Sequence
GTGAAATCCA GTATCATTTC TTTTCTCACG CCCAGCTACA GCAAGGTCAG TCATGGACAC CCAACAACGC ATCAAGCAAA TCGTCGACGA AAACCCGGTC GTGCTCTTCA TGAAAGGCAC CGCGCAATTC CCGATGTGCG GCTTTTCGGG CCGCGCCGTC CAGGTGCTGA AGGCGTGCGG CGTCGACCAG TTCAAGACGG TCAACGTCCT CGAAGACGAA GAGATCCGCC AGGGCATCAA GGAGTTCTCG AACTGGCCGA CGATCCCGCA GCTCTACGTG AAGGGCGAAT TCGTCGGCGG CTCGGACATC ATGATGGAGA TGTATCAGTC GGGCGAACTG CAGCAACTGT TCACCGCCTG ATTTTCCGCC CTGCGATGGA GCCCCGTCCG GCGCCGCCGC GGCGCCTGAT CGTCGCGATC ACGGGTGCCA CCGGCGCGAT CTATGGCGTG CGCATGCTCG ACATGCTGCG CGCGTCGGGC GGCGTCGAGA CGCACCTGCT GATCTCGAGC GCCGGCTGGC TCAACATCCG GCACGAACTG CAACTGTCGA AGGACGACGT GCTGGCGCGC GCCGACGTCG TCCATTCGGT GCGCGACGTC GGCGCGAGCA TTGCATCCGG CTCGTTCGCG ACCGAGGGCA TGATCGTCGC GCCCTGCTCG ATGAAGACGC TCGCGAGCAT CGCGCACGGG CTATCCGACA ATCTCATCAC CCGCGCCGCC GACGTCGCGC TGAAAGAGCG CCGCCGGCTC GTGCTGCTCG TGCGCGAGGC GCCGTTCAAC CTCGCGCACC TACGCAACAT GACGGCCGTC ACCGAGATGG GCGGCGTGAT CTTCCCGCCG GTGCCCGCGT TCTACGCGAT GCCGAAGACG ATCGACGAGA TCGTCGACCA TACGGTCGCG CGCGTGCTCG ACATGTTCGC GCTCGGCGCG CCGCTCACCG CGCCGTGGCA AGGGCTGCGC GAGCACGGC
Details for BupsA.00810.a.A1.GE30918
HARVESTED ON: 11/23/2010
SEQUENCED ON: 12/6/2010
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 38
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKSSIISF LTPSYSKVSH GHPTTHXANR XRKPGRALHE RHRAIPDVXL FGPRXPGXEG VRRXPVQDXX RPXRRRDPXG XXGVLELADD PXXLREGRIX XXLGHXXXXX XXXXXXAXXX XXIFXXAMXX XPXPPXXXXR XXXRXXRPIX XXXXLDXXRA SGXXXTXLLI XXAXXXXIRX XAXXXXXXAW XXXTXXXXXX XXXEXCIXXX XXXAXXXXXX XXXXXSXXRX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX PXXXAXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXGXXX XXX
Validated NT Sequence
attttgttta ctttaagaag gaganatacn atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga aatccagtat catttctttt ctcacgccca gctacagcaa ggtcagtcat ggacacccaa caacgcatca ngcaaatcgt cnacgaaaac ccggtcgtgc tcttcatgaa aggcaccgcg caattcccga tgtgnggctt ttcgggccgc gccntccagg tnctgaaggn gtgcggcgtc naccagttca agacngnnna cgtcctcnaa gacgaagaga tccnnnnggg nntcnnggag ttctcgaact ggccgacgat cccncanctc ttcgtgaagg gcgaattcnt cnnnnnctcg gacatcangn ngnnnnnnnn tnngncnggn nnanngnngc aanngntcnn cncnngattt tccnncntgc gatgganncc cntccggngc cgccncnnnn ncngnancgn nncgntcncc ggngcnnncg gccgatctnt ngnntnnncn tgctcgacnt gnngcgcgcg tcgggcngnn ncnagacnca nctgctgatc tnnagngccn gnnnnntcna catccggcnc nnngcnnctg nnganngnnn acntgcntgg ngcgnnncna cntnnnncnt cngnnnnngn nnnnngcncg agnnntgcat cnnnntcnnn nnnnannnng cnnnnnannn nnnncnnnnn nnanannana nnntntcgnn nntccgtnnn ngnnnntnnn gnnntcnnnn cnnnnnnnnn ncnnnanatn nngantnnna nagnnnnnnn nnnncncngn ctnnnncnng nnnnnnnnnn ccnnnnnctc nngcnncnna nnnnnnannn nggngntnnn cnnnatgnnn gannnnnncn nnnnnnnnnn nnnnnnnnnc nnnnnnnann nnnnnngngn nnnnnntnnn nnnnnnnnnn nnnnnngnnn nnnaannnnn nnnnnngnnn nnnnnnnnnn nnnnnnnngg nnnnnnngnn ncnnnnnnnn ng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKSSIISF LTPSYSKVSH GHPTTHQANR RRKPGRALHE RHRAIPDVRL FGPRRPGAEG VRRRPVQDGQ RPRRRRDPPG HQGVLELADD PAALREGRIR RRLGHHDGDV SVGRTAATVH RLIFRPAMEP RPAPPRRLIV AITGATGAIY GVRMLDMLRA SGGVETHLLI SSAGWLNIRH ELQLSKDDVL ARADVVHSVR DVGASIASGS FATEGMIVAP CSMKTLASIA HGLSDNLITR AADVALKERR RLVLLVREAP FNLAHLRNMT AVTEMGGVIF PPVPAFYAMP KTIDEIVDHT VARVLDMFAL GAPLTAPWQG LREHG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaatcc agtatcattt cttttctcac gcccagctac agcaaggtca gtcatggaca cccaacaacg catcaagcaa atcgtcgacg aaaacccggt cgtgctcttc atgaaaggca ccgcgcaatt cccgatgtgc ggcttttcgg gccgcgccgt ccaggtgctg aaggcgtgcg gcgtcgacca gttcaagacg gtcaacgtcc tcgaagacga agagatccgc cagggcatca aggagttctc gaactggccg acgatcccgc agctctacgt gaagggcgaa ttcgtcggcg gctcggacat catgatggag atgtatcagt cgggcgaact gcagcaactg ttcaccgcct gattttccgc cctgcgatgg agccccgtcc ggcgccgccg cggcgcctga tcgtcgcgat cacgggtgcc accggcgcga tctatggcgt gcgcatgctc gacatgctgc gcgcgtcggg cggcgtcgag acgcacctgc tgatctcgag cgccggctgg ctcaacatcc ggcacgaact gcaactgtcg aaggacgacg tgctggcgcg cgccgacgtc gtccattcgg tgcgcgacgt cggcgcgagc attgcatccg gctcgttcgc gaccgagggc atgatcgtcg cgccctgctc gatgaagacg ctcgcgagca tcgcgcacgg gctatccgac aatctcatca cccgcgccgc cgacgtcgcg ctgaaagagc gccgccggct cgtgctgctc gtgcgcgagg cgccgttcaa cctcgcgcac ctacgcaaca tgacggccgt caccgagatg ggcggcgtga tcttcccgcc ggtgcccgcg ttctacgcga tgccgaagac gatcgacgag atcgtcgacc atacggtcgc gcgcgtgctc gacatgttcg cgctcggcgc gccgctcacc gcgccgtggc aagggctgcg cgagcacggc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa