BupsA.00629.a

Medium-chain-fatty-acid--CoA ligase

CENTER ID: BupsA.00629.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00629.a.A2.GE32543 Mature protein( BupsA.00629.a ) 27 548
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2567
RefSeq: YP_333851.1
UniProt: Q3JRF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKPLLGQMM DMPLLVSSLI AHAARHAGDV EIVSRRVEGD IHRYTYRDCE ARSKRLAQAL TGLGVGVGVG ERIGTLAWNG YRHVEAYYGI SGMGAVCHTI NPRLFPEQIA YIVNHAEDRY VLFDLTFAPL VDQLAPQCPN VKGWIAMTDD AHLPKGATPY LCYETLVGAQ DGDYAWPLLD ERQASSLCYT SGTTGHPKGA LYSHRSTVLH AYGAALPDAM GLSSRDAALP VVPMFHVNAW GLPYTAALTG TKLVLPGKDL DGKSLYELIE SERVTFSAGV PTVWLGLLGY MREAGVRFST LERTVIGGSA CPPSMLETFE DVYDVRVIHA WGMTELSPLG TLAKLNWAQS QRGIGEQRRL LEKQGRVIYG IDMRIVGEDG RELPWDGVAF GDLQVRGPWV IDRYFGIDAS PLVDGWFPTG DVATIDADGF LQITDRSKDV IKSGGEWISS IDVENVAVAH PAVAEAACIA CAHPKWTERP LLVVVKRAGM DVTRDELLAF YEGKVAKWWI PDDVAFVDAL PHTATGKLQK LKLREQFRGH VLPTAVDA
NT Sequence
ATGGGTAAGC CGTTGCTGGG CCAGATGATG GATATGCCGC TGCTCGTGTC GTCGCTGATC GCGCATGCGG CGCGACACGC GGGTGACGTC GAGATCGTGT CCCGGCGCGT CGAGGGCGAC ATTCACCGCT ATACCTATCG CGATTGCGAA GCGCGTTCCA AACGGCTCGC GCAGGCGCTG ACCGGCCTGG GCGTGGGCGT GGGCGTGGGC GAGCGCATTG GAACGCTCGC GTGGAACGGC TACCGGCACG TCGAGGCGTA TTACGGCATC AGCGGGATGG GCGCCGTCTG CCATACGATC AACCCGCGCC TCTTTCCCGA GCAGATCGCG TACATCGTCA ACCACGCCGA GGATCGATAC GTGCTGTTCG ACCTCACCTT CGCGCCGCTC GTCGATCAGC TCGCGCCGCA ATGCCCGAAC GTGAAGGGCT GGATCGCGAT GACGGACGAC GCGCATCTGC CGAAGGGCGC GACGCCTTAT CTCTGTTACG AGACGCTCGT CGGCGCGCAG GACGGCGATT ACGCCTGGCC GCTGCTCGAC GAGCGGCAGG CGTCGTCGCT CTGCTACACG TCCGGCACGA CGGGCCACCC GAAGGGCGCG CTCTATTCGC ACCGCTCGAC GGTGCTGCAC GCGTACGGCG CGGCGCTGCC CGACGCGATG GGGCTGTCCT CGCGCGACGC GGCGCTGCCC GTCGTGCCGA TGTTCCACGT CAACGCATGG GGGCTGCCGT ACACGGCGGC GCTCACCGGC ACGAAGCTCG TGCTGCCCGG CAAGGACCTC GACGGCAAAT CGCTGTACGA GCTGATCGAA AGCGAGCGCG TGACGTTCTC GGCGGGCGTG CCCACCGTCT GGCTCGGTCT GCTCGGGTAC ATGCGCGAGG CCGGCGTGCG CTTCTCGACG CTCGAGCGCA CGGTGATCGG CGGCTCCGCG TGCCCGCCTT CGATGCTCGA GACGTTCGAG GACGTCTACG ACGTGCGTGT GATCCATGCA TGGGGGATGA CCGAGCTGTC GCCGCTCGGC ACGCTGGCCA AGCTCAATTG GGCGCAGTCG CAGCGCGGCA TCGGCGAGCA GCGGCGGCTC CTCGAGAAGC AGGGCCGGGT GATCTACGGG ATCGATATGC GCATCGTCGG CGAGGACGGC CGCGAGTTGC CGTGGGACGG CGTCGCGTTC GGCGACTTGC AGGTGCGCGG GCCGTGGGTG ATCGACCGCT ATTTCGGAAT CGACGCGTCG CCGCTCGTCG ACGGCTGGTT CCCGACGGGC GACGTCGCGA CGATCGACGC CGACGGCTTC CTGCAGATCA CCGATCGCAG CAAGGACGTG ATCAAGTCGG GCGGCGAATG GATCAGCTCG ATCGACGTCG AGAACGTCGC GGTGGCGCAC CCGGCCGTCG CGGAGGCCGC GTGCATCGCA TGCGCGCATC CGAAATGGAC CGAGCGGCCG CTCCTCGTCG TCGTCAAGCG CGCCGGCATG GACGTGACGC GCGACGAACT GCTCGCGTTC TACGAGGGCA AGGTCGCGAA ATGGTGGATT CCGGACGATG TCGCGTTCGT CGACGCGCTG CCGCACACCG CGACGGGCAA GCTGCAAAAG CTCAAGCTGC GCGAGCAATT CCGGGGCCAC GTGCTGCCGA CGGCCGTGGA CGCG
Details for BupsA.00629.a.A2.GE32543
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 59kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 72
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGDVEIVS RRVEGDIHRY TYRDCEARSK RLAQALTGLG VGVGVGERIG TLAWNGYRHV EAYYGISGMG AVCHTINPRL FPEQIAYIVN HAEDRYVLFD LTFAPLVDQL APQCPNVKGW IAMTDDAHLP KGATPYLCYE TLVGAQDGDY AWPLLDERQA SSLCYTSGTT GHPKGALYSH RSTVLHAYGA ALPDAMGLSS RDAALPVVPM FHVNAWGLPY TAALTGTKLV LPGKDLDGKS LYELIESERV TFSAGVPTVW LGLLGYXREA GVRFSTLERT VIGGSACPPS MLETFEDVYX XRVIHAWGXX ELXXSXXXXX XXXXSXXXXR XXXXXXXXXX XDLXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
gnnnnnnnnn ngnnnnnnct ttaanaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcgggt gacgtcgaga tcgtgtcccg gcgcgtcgag ggcgacattc accgctatac ctatcgcgat tgcgaagcgc gttccaaacg gctcgcgcag gcgctgaccg gcctgggcgt gggcgtgggc gtgggcgagc gcattggaac gctcgcgtgg aacggctacc ggcacgtcga ggcgtattac ggcatcagcg ggatgggcgc cgtctgccat acgatcaacc cgcgcctctt tcccgagcag atcgcgtaca tcgtcaacca cgccgaggat cgatacgtgc tgttcgacct caccttcgcg ccgctcgtcg atcagctcgc gccgcaatgc ccgaacgtga agggctggat cgcgatgacg gacgacgcgc atctgccgaa gggcgcgacg ccttatctct gttacgagac gctcgtcggc gcgcaggacg gcgattacgc ctggccgctg ctcgacgagc ggcaggcgtc gtcgctctgc tacacgtccg gcacgacggg ccacccgaag ggcgcgctct attcgcaccg ctcgacggtg ctgcacgcgt acggcgcggc gctgcccgac gcgatggggc tgtcctcgcg cgacgcggcg ctgcccgtcg tgccgatgtt ccacgtcaac gcatgggggc tgccgtacac ggcggcgctc accggcacga agctcgtgct gcccggcaag gacctcgacg gcaaatcgct gtacgagctg atcgaaagcg agcgcgtgac gttctcggcg ggcgtgccca ccgtctggct cggtctgctc gggtacntgc gcgaggcngg cgtgcgcttc tcgacgctcg agcgcacggt gatcggcggc tccgcgtgcc cgccttcgat gctcgagacg ttcgaggacg tctacnacnt gcgtgtgatc catgcatggg ggangancga gctgncncnc tcnnnannnn nnnnnnctcn nnnggngcan tcncancnnn ngnatcgnnn nncnnnnnnn gnnnnnnnnn nnnnnnnnnn ngatctacna nntgnncnnn nnnnnnnngn nngccnnnnn nnnnnnngnn nnnnncnnnn nnnnnnnnnn nnnnnnnngn ncnnngnnnn nnnnnncgnn nnnnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGDVEIVS RRVEGDIHRY TYRDCEARSK RLAQALTGLG VGVGVGERIG TLAWNGYRHV EAYYGISGMG AVCHTINPRL FPEQIAYIVN HAEDRYVLFD LTFAPLVDQL APQCPNVKGW IAMTDDAHLP KGATPYLCYE TLVGAQDGDY AWPLLDERQA SSLCYTSGTT GHPKGALYSH RSTVLHAYGA ALPDAMGLSS RDAALPVVPM FHVNAWGLPY TAALTGTKLV LPGKDLDGKS LYELIESERV TFSAGVPTVW LGLLGYMREA GVRFSTLERT VIGGSACPPS MLETFEDVYD VRVIHAWGMT ELSPLGTLAK LNWAQSQRGI GEQRRLLEKQ GRVIYGIDMR IVGEDGRELP WDGVAFGDLQ VRGPWVIDRY FGIDASPLVD GWFPTGDVAT IDADGFLQIT DRSKDVIKSG GEWISSIDVE NVAVAHPAVA EAACIACAHP KWTERPLLVV VKRAGMDVTR DELLAFYEGK VAKWWIPDDV AFVDALPHTA TGKLQKLKLR EQFRGHVLPT AVDA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgggtgac gtcgagatcg tgtcccggcg cgtcgagggc gacattcacc gctataccta tcgcgattgc gaagcgcgtt ccaaacggct cgcgcaggcg ctgaccggcc tgggcgtggg cgtgggcgtg ggcgagcgca ttggaacgct cgcgtggaac ggctaccggc acgtcgaggc gtattacggc atcagcggga tgggcgccgt ctgccatacg atcaacccgc gcctctttcc cgagcagatc gcgtacatcg tcaaccacgc cgaggatcga tacgtgctgt tcgacctcac cttcgcgccg ctcgtcgatc agctcgcgcc gcaatgcccg aacgtgaagg gctggatcgc gatgacggac gacgcgcatc tgccgaaggg cgcgacgcct tatctctgtt acgagacgct cgtcggcgcg caggacggcg attacgcctg gccgctgctc gacgagcggc aggcgtcgtc gctctgctac acgtccggca cgacgggcca cccgaagggc gcgctctatt cgcaccgctc gacggtgctg cacgcgtacg gcgcggcgct gcccgacgcg atggggctgt cctcgcgcga cgcggcgctg cccgtcgtgc cgatgttcca cgtcaacgca tgggggctgc cgtacacggc ggcgctcacc ggcacgaagc tcgtgctgcc cggcaaggac ctcgacggca aatcgctgta cgagctgatc gaaagcgagc gcgtgacgtt ctcggcgggc gtgcccaccg tctggctcgg tctgctcggg tacatgcgcg aggccggcgt gcgcttctcg acgctcgagc gcacggtgat cggcggctcc gcgtgcccgc cttcgatgct cgagacgttc gaggacgtct acgacgtgcg tgtgatccat gcatggggga tgaccgagct gtcgccgctc ggcacgctgg ccaagctcaa ttgggcgcag tcgcagcgcg gcatcggcga gcagcggcgg ctcctcgaga agcagggccg ggtgatctac gggatcgata tgcgcatcgt cggcgaggac ggccgcgagt tgccgtggga cggcgtcgcg ttcggcgact tgcaggtgcg cgggccgtgg gtgatcgacc gctatttcgg aatcgacgcg tcgccgctcg tcgacggctg gttcccgacg ggcgacgtcg cgacgatcga cgccgacggc ttcctgcaga tcaccgatcg cagcaaggac gtgatcaagt cgggcggcga atggatcagc tcgatcgacg tcgagaacgt cgcggtggcg cacccggccg tcgcggaggc cgcgtgcatc gcatgcgcgc atccgaaatg gaccgagcgg ccgctcctcg tcgtcgtcaa gcgcgccggc atggacgtga cgcgcgacga actgctcgcg ttctacgagg gcaaggtcgc gaaatggtgg attccggacg atgtcgcgtt cgtcgacgcg ctgccgcaca ccgcgacggg caagctgcaa aagctcaagc tgcgcgagca attccggggc cacgtgctgc cgacggccgt ggacgcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa