BupsA.00624.a

ABC transporter, substrate binding protein

CENTER ID: BupsA.00624.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00624.a.A2.GE30911 Mature protein( BupsA.00624.a ) 32 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00624.a.A2.PW30799 Mature protein( BupsA.00624.a ) 32 351
BupsA.00624.a.A2.PW31844 Mature protein( BupsA.00624.a ) 32 351

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1558
RefSeq: YP_332916.1
UniProt: Q3JU37

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFVKQARPLL RTLLRPLAFA FALAAVAPAA HAANEVNLYT TREPKLIQPL VDAFTKQSGI AVNTVFVKDG LLERVKAEGA RSPADVLMTV DIGNLLDLVD GGLTQPVRSA SLDGAIPANL RGAGGNWYAL SLRDRVLYVD KNMQLDAITY ESLADPKWKG KVCIRSGQHP YNTALVAAMI AHDGEAAAEQ WLRGVKANLA RKATGGDRDV ARDILGGICD IGLANAYYAG HMKHAQPGTD ARKWGDAIKV VRPTFANAKS GGTHVNVSGA AVAKHAPNRD NAVKLLEYLA SPPAQALYAQ ANYEYPVRAG VALDPVIAGF GPLKVDPLPL VEIAKYRERA SQLVDKVGFD N
NT Sequence
ATGTTCGTCA AGCAAGCCCG CCCGCTGCTG CGCACGCTGC TGCGCCCGCT CGCGTTCGCA TTCGCGCTCG CCGCCGTCGC GCCCGCCGCG CACGCGGCCA ACGAAGTCAA CCTGTACACG ACCCGCGAGC CGAAGCTGAT CCAGCCGCTC GTCGACGCGT TCACGAAGCA AAGCGGCATC GCCGTCAACA CGGTGTTCGT CAAGGACGGC CTGCTCGAGC GCGTGAAGGC CGAAGGCGCG CGCTCGCCCG CCGACGTGCT GATGACGGTC GACATCGGCA ATCTGCTCGA TCTCGTCGAC GGCGGGCTCA CGCAGCCCGT GCGCTCGGCG TCGCTCGACG GCGCGATTCC CGCGAACCTG CGCGGCGCGG GCGGCAACTG GTACGCGCTG TCGCTGCGCG ACCGCGTGCT GTACGTCGAC AAGAACATGC AGCTCGACGC GATCACGTAC GAGTCGCTCG CCGATCCGAA ATGGAAGGGC AAGGTCTGCA TCCGTTCCGG CCAGCATCCG TACAACACGG CGCTCGTCGC CGCGATGATC GCGCACGACG GCGAAGCCGC GGCCGAGCAA TGGCTGCGCG GCGTGAAGGC GAATCTCGCG CGCAAGGCGA CGGGCGGCGA TCGCGACGTC GCGCGCGACA TCCTCGGCGG CATCTGCGAC ATCGGGCTCG CGAACGCGTA CTACGCCGGC CACATGAAGC ACGCGCAGCC CGGCACCGAC GCGCGCAAGT GGGGCGACGC GATCAAGGTC GTGCGCCCGA CGTTCGCGAA CGCGAAGAGC GGCGGCACGC ATGTGAACGT GAGCGGCGCG GCCGTCGCGA AGCACGCGCC GAACAGGGAC AACGCGGTGA AGCTGCTCGA ATACCTCGCG TCGCCGCCCG CGCAGGCGCT CTACGCACAG GCGAACTACG AATACCCGGT GCGCGCGGGC GTCGCGCTCG ATCCGGTGAT CGCCGGCTTC GGGCCGCTGA AGGTCGATCC GCTGCCGCTC GTCGAGATCG CGAAGTACCG CGAGCGCGCC AGCCAGCTCG TCGACAAGGT CGGCTTTGAC AAC
Details for BupsA.00624.a.A2.GE30911
HARVESTED ON: 11/23/2010
SEQUENCED ON: 10/29/2014
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAANEVNLY TTREPKLIQP LVDAFTKQSG IAVNTVFVKD GLLERVKAEG ARSPADVLMT VDIGNLLDLV DGGLTQPVRS ASLDGAIPAN LRGAGGNWYA LSLRDRVLYV DKNMQLDAIT YESLADPKWK GKVCIRSGQH PYNTALVAAM IAHDGEAAAE QWLRGVKANL ARKATGGDRD VARDILGGIC DIGLANAYYA GHMKHAQPGT DARKWGDAIK VVRPTFANAK SGGTHVNVSG AAVAKHAPNR DNAVKLLEYL ASPPAQALYA QANYEYPVRA GVALDPVIAG FGPLKVDPLP LVEIAKYRER ASQLVDKVGF
Validated NT Sequence
caaagccgac cttgtcgacg agctggctgg cgcgctcgcg gtacttcgcg atctcgacga gcggcagcgg atcgaccttc agcggcccga agccggcgat caccggatcg agcgcgacgc ccgcgcgcac cgggtattcg tagttcgcct gtgcgtagag cgcctgcgca ggcggcgacg cgaggtattc gagcagcttc accgcgttgt ccctgttcgg cgcgtgcttc gcgacggccg cgccgctcac gttcacatgc gtgccgccgc tcttcgcgtt cgcgaacgtc gggcgcacga ccttgatcgc gtcgccccac ttgcgcgcgt cggtgccggg ctgcgcgtgc ttcatgtggc cggcgtagta cgcgttcgcg agcccgatgt cgcagatgcc gccgaggatg tcgcgcgcga cgtcgcgatc gccgcccgtc gccttgcgcg cgagattcgc cttcacgccg cgcagccatt gctcggccgc ggcttcgccg tcgtgcgcga tcatcgcggc gacgagcgcc gtgttgtacg gatgctggcc ggaacggatg cagaccttgc ccttccattt cggatcggcg agcgactcgt acgtgatcgc gtcgagctgc atgttcttgt cgacgtacag cacgcggtcg cgcagcgaca gcgcgtacca gttgccgccc gcgccgcgca ggttcgcggg aatcgcgccg tcgagcgacg ccgagcgcac gggctgcgtg agcccgccgt cgacgagatc gagcagattg ccgatgtcga ccgtcatcag cacgtcggcg ggcgagcgcg cgccttcggc cttcacgcgc tcgagcaggc cgtccttgac gaacaccgtg ttgacggcga tgccgctttg cttcgtgaac gcgtcgacga gcggctggat cagcttcggc tcgcgggtcg tgtacaggtt gacttcgttg gccgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAANEVNLY TTREPKLIQP LVDAFTKQSG IAVNTVFVKD GLLERVKAEG ARSPADVLMT VDIGNLLDLV DGGLTQPVRS ASLDGAIPAN LRGAGGNWYA LSLRDRVLYV DKNMQLDAIT YESLADPKWK GKVCIRSGQH PYNTALVAAM IAHDGEAAAE QWLRGVKANL ARKATGGDRD VARDILGGIC DIGLANAYYA GHMKHAQPGT DARKWGDAIK VVRPTFANAK SGGTHVNVSG AAVAKHAPNR DNAVKLLEYL ASPPAQALYA QANYEYPVRA GVALDPVIAG FGPLKVDPLP LVEIAKYRER ASQLVDKVGF DN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccaac gaagtcaacc tgtacacgac ccgcgagccg aagctgatcc agccgctcgt cgacgcgttc acgaagcaaa gcggcatcgc cgtcaacacg gtgttcgtca aggacggcct gctcgagcgc gtgaaggccg aaggcgcgcg ctcgcccgcc gacgtgctga tgacggtcga catcggcaat ctgctcgatc tcgtcgacgg cgggctcacg cagcccgtgc gctcggcgtc gctcgacggc gcgattcccg cgaacctgcg cggcgcgggc ggcaactggt acgcgctgtc gctgcgcgac cgcgtgctgt acgtcgacaa gaacatgcag ctcgacgcga tcacgtacga gtcgctcgcc gatccgaaat ggaagggcaa ggtctgcatc cgttccggcc agcatccgta caacacggcg ctcgtcgccg cgatgatcgc gcacgacggc gaagccgcgg ccgagcaatg gctgcgcggc gtgaaggcga atctcgcgcg caaggcgacg ggcggcgatc gcgacgtcgc gcgcgacatc ctcggcggca tctgcgacat cgggctcgcg aacgcgtact acgccggcca catgaagcac gcgcagcccg gcaccgacgc gcgcaagtgg ggcgacgcga tcaaggtcgt gcgcccgacg ttcgcgaacg cgaagagcgg cggcacgcat gtgaacgtga gcggcgcggc cgtcgcgaag cacgcgccga acagggacaa cgcggtgaag ctgctcgaat acctcgcgtc gccgcccgcg caggcgctct acgcacaggc gaactacgaa tacccggtgc gcgcgggcgt cgcgctcgat ccggtgatcg ccggcttcgg gccgctgaag gtcgatccgc tgccgctcgt cgagatcgcg aagtaccgcg agcgcgccag ccagctcgtc gacaaggtcg gctttgacaa ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BupsA.00624.a.A2.PW30799
PURIFICATION DATe: 2/25/2011
CONCENTRATION: 45.16mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAANEVNLY TTREPKLIQP LVDAFTKQSG IAVNTVFVKD GLLERVKAEG ARSPADVLMT VDIGNLLDLV DGGLTQPVRS ASLDGAIPAN LRGAGGNWYA LSLRDRVLYV DKNMQLDAIT YESLADPKWK GKVCIRSGQH PYNTALVAAM IAHDGEAAAE QWLRGVKANL ARKATGGDRD VARDILGGIC DIGLANAYYA GHMKHAQPGT DARKWGDAIK VVRPTFANAK SGGTHVNVSG AAVAKHAPNR DNAVKLLEYL ASPPAQALYA QANYEYPVRA GVALDPVIAG FGPLKVDPLP LVEIAKYRER ASQLVDKVGF
Validated NT Sequence
caaagccgac cttgtcgacg agctggctgg cgcgctcgcg gtacttcgcg atctcgacga gcggcagcgg atcgaccttc agcggcccga agccggcgat caccggatcg agcgcgacgc ccgcgcgcac cgggtattcg tagttcgcct gtgcgtagag cgcctgcgca ggcggcgacg cgaggtattc gagcagcttc accgcgttgt ccctgttcgg cgcgtgcttc gcgacggccg cgccgctcac gttcacatgc gtgccgccgc tcttcgcgtt cgcgaacgtc gggcgcacga ccttgatcgc gtcgccccac ttgcgcgcgt cggtgccggg ctgcgcgtgc ttcatgtggc cggcgtagta cgcgttcgcg agcccgatgt cgcagatgcc gccgaggatg tcgcgcgcga cgtcgcgatc gccgcccgtc gccttgcgcg cgagattcgc cttcacgccg cgcagccatt gctcggccgc ggcttcgccg tcgtgcgcga tcatcgcggc gacgagcgcc gtgttgtacg gatgctggcc ggaacggatg cagaccttgc ccttccattt cggatcggcg agcgactcgt acgtgatcgc gtcgagctgc atgttcttgt cgacgtacag cacgcggtcg cgcagcgaca gcgcgtacca gttgccgccc gcgccgcgca ggttcgcggg aatcgcgccg tcgagcgacg ccgagcgcac gggctgcgtg agcccgccgt cgacgagatc gagcagattg ccgatgtcga ccgtcatcag cacgtcggcg ggcgagcgcg cgccttcggc cttcacgcgc tcgagcaggc cgtccttgac gaacaccgtg ttgacggcga tgccgctttg cttcgtgaac gcgtcgacga gcggctggat cagcttcggc tcgcgggtcg tgtacaggtt gacttcgttg gccgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAANEVNLY TTREPKLIQP LVDAFTKQSG IAVNTVFVKD GLLERVKAEG ARSPADVLMT VDIGNLLDLV DGGLTQPVRS ASLDGAIPAN LRGAGGNWYA LSLRDRVLYV DKNMQLDAIT YESLADPKWK GKVCIRSGQH PYNTALVAAM IAHDGEAAAE QWLRGVKANL ARKATGGDRD VARDILGGIC DIGLANAYYA GHMKHAQPGT DARKWGDAIK VVRPTFANAK SGGTHVNVSG AAVAKHAPNR DNAVKLLEYL ASPPAQALYA QANYEYPVRA GVALDPVIAG FGPLKVDPLP LVEIAKYRER ASQLVDKVGF DN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCCAAC GAAGTCAACC TGTACACGAC CCGCGAGCCG AAGCTGATCC AGCCGCTCGT CGACGCGTTC ACGAAGCAAA GCGGCATCGC CGTCAACACG GTGTTCGTCA AGGACGGCCT GCTCGAGCGC GTGAAGGCCG AAGGCGCGCG CTCGCCCGCC GACGTGCTGA TGACGGTCGA CATCGGCAAT CTGCTCGATC TCGTCGACGG CGGGCTCACG CAGCCCGTGC GCTCGGCGTC GCTCGACGGC GCGATTCCCG CGAACCTGCG CGGCGCGGGC GGCAACTGGT ACGCGCTGTC GCTGCGCGAC CGCGTGCTGT ACGTCGACAA GAACATGCAG CTCGACGCGA TCACGTACGA GTCGCTCGCC GATCCGAAAT GGAAGGGCAA GGTCTGCATC CGTTCCGGCC AGCATCCGTA CAACACGGCG CTCGTCGCCG CGATGATCGC GCACGACGGC GAAGCCGCGG CCGAGCAATG GCTGCGCGGC GTGAAGGCGA ATCTCGCGCG CAAGGCGACG GGCGGCGATC GCGACGTCGC GCGCGACATC CTCGGCGGCA TCTGCGACAT CGGGCTCGCG AACGCGTACT ACGCCGGCCA CATGAAGCAC GCGCAGCCCG GCACCGACGC GCGCAAGTGG GGCGACGCGA TCAAGGTCGT GCGCCCGACG TTCGCGAACG CGAAGAGCGG CGGCACGCAT GTGAACGTGA GCGGCGCGGC CGTCGCGAAG CACGCGCCGA ACAGGGACAA CGCGGTGAAG CTGCTCGAAT ACCTCGCGTC GCCGCCCGCG CAGGCGCTCT ACGCACAGGC GAACTACGAA TACCCGGTGC GCGCGGGCGT CGCGCTCGAT CCGGTGATCG CCGGCTTCGG GCCGCTGAAG GTCGATCCGC TGCCGCTCGT CGAGATCGCG AAGTACCGCG AGCGCGCCAG CCAGCTCGTC GACAAGGTCG GCTTTGACAA CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for BupsA.00624.a.A2.PW31844
PURIFICATION DATe: 2/25/2011
CONCENTRATION: 21.77mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 35
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAANEVNLY TTREPKLIQP LVDAFTKQSG IAVNTVFVKD GLLERVKAEG ARSPADVLMT VDIGNLLDLV DGGLTQPVRS ASLDGAIPAN LRGAGGNWYA LSLRDRVLYV DKNMQLDAIT YESLADPKWK GKVCIRSGQH PYNTALVAAM IAHDGEAAAE QWLRGVKANL ARKATGGDRD VARDILGGIC DIGLANAYYA GHMKHAQPGT DARKWGDAIK VVRPTFANAK SGGTHVNVSG AAVAKHAPNR DNAVKLLEYL ASPPAQALYA QANYEYPVRA GVALDPVIAG FGPLKVDPLP LVEIAKYRER ASQLVDKVGF
Validated NT Sequence
caaagccgac cttgtcgacg agctggctgg cgcgctcgcg gtacttcgcg atctcgacga gcggcagcgg atcgaccttc agcggcccga agccggcgat caccggatcg agcgcgacgc ccgcgcgcac cgggtattcg tagttcgcct gtgcgtagag cgcctgcgca ggcggcgacg cgaggtattc gagcagcttc accgcgttgt ccctgttcgg cgcgtgcttc gcgacggccg cgccgctcac gttcacatgc gtgccgccgc tcttcgcgtt cgcgaacgtc gggcgcacga ccttgatcgc gtcgccccac ttgcgcgcgt cggtgccggg ctgcgcgtgc ttcatgtggc cggcgtagta cgcgttcgcg agcccgatgt cgcagatgcc gccgaggatg tcgcgcgcga cgtcgcgatc gccgcccgtc gccttgcgcg cgagattcgc cttcacgccg cgcagccatt gctcggccgc ggcttcgccg tcgtgcgcga tcatcgcggc gacgagcgcc gtgttgtacg gatgctggcc ggaacggatg cagaccttgc ccttccattt cggatcggcg agcgactcgt acgtgatcgc gtcgagctgc atgttcttgt cgacgtacag cacgcggtcg cgcagcgaca gcgcgtacca gttgccgccc gcgccgcgca ggttcgcggg aatcgcgccg tcgagcgacg ccgagcgcac gggctgcgtg agcccgccgt cgacgagatc gagcagattg ccgatgtcga ccgtcatcag cacgtcggcg ggcgagcgcg cgccttcggc cttcacgcgc tcgagcaggc cgtccttgac gaacaccgtg ttgacggcga tgccgctttg cttcgtgaac gcgtcgacga gcggctggat cagcttcggc tcgcgggtcg tgtacaggtt gacttcgttg gccgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAANEVNLY TTREPKLIQP LVDAFTKQSG IAVNTVFVKD GLLERVKAEG ARSPADVLMT VDIGNLLDLV DGGLTQPVRS ASLDGAIPAN LRGAGGNWYA LSLRDRVLYV DKNMQLDAIT YESLADPKWK GKVCIRSGQH PYNTALVAAM IAHDGEAAAE QWLRGVKANL ARKATGGDRD VARDILGGIC DIGLANAYYA GHMKHAQPGT DARKWGDAIK VVRPTFANAK SGGTHVNVSG AAVAKHAPNR DNAVKLLEYL ASPPAQALYA QANYEYPVRA GVALDPVIAG FGPLKVDPLP LVEIAKYRER ASQLVDKVGF DN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCCAAC GAAGTCAACC TGTACACGAC CCGCGAGCCG AAGCTGATCC AGCCGCTCGT CGACGCGTTC ACGAAGCAAA GCGGCATCGC CGTCAACACG GTGTTCGTCA AGGACGGCCT GCTCGAGCGC GTGAAGGCCG AAGGCGCGCG CTCGCCCGCC GACGTGCTGA TGACGGTCGA CATCGGCAAT CTGCTCGATC TCGTCGACGG CGGGCTCACG CAGCCCGTGC GCTCGGCGTC GCTCGACGGC GCGATTCCCG CGAACCTGCG CGGCGCGGGC GGCAACTGGT ACGCGCTGTC GCTGCGCGAC CGCGTGCTGT ACGTCGACAA GAACATGCAG CTCGACGCGA TCACGTACGA GTCGCTCGCC GATCCGAAAT GGAAGGGCAA GGTCTGCATC CGTTCCGGCC AGCATCCGTA CAACACGGCG CTCGTCGCCG CGATGATCGC GCACGACGGC GAAGCCGCGG CCGAGCAATG GCTGCGCGGC GTGAAGGCGA ATCTCGCGCG CAAGGCGACG GGCGGCGATC GCGACGTCGC GCGCGACATC CTCGGCGGCA TCTGCGACAT CGGGCTCGCG AACGCGTACT ACGCCGGCCA CATGAAGCAC GCGCAGCCCG GCACCGACGC GCGCAAGTGG GGCGACGCGA TCAAGGTCGT GCGCCCGACG TTCGCGAACG CGAAGAGCGG CGGCACGCAT GTGAACGTGA GCGGCGCGGC CGTCGCGAAG CACGCGCCGA ACAGGGACAA CGCGGTGAAG CTGCTCGAAT ACCTCGCGTC GCCGCCCGCG CAGGCGCTCT ACGCACAGGC GAACTACGAA TACCCGGTGC GCGCGGGCGT CGCGCTCGAT CCGGTGATCG CCGGCTTCGG GCCGCTGAAG GTCGATCCGC TGCCGCTCGT CGAGATCGCG AAGTACCGCG AGCGCGCCAG CCAGCTCGTC GACAAGGTCG GCTTTGACAA CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA