BupsA.00367.b

RNA polymerase sigma factor

CENTER ID: BupsA.00367.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00367.b.A1.GE32540 Full length( BupsA.00367.b ) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.741
RefSeq: YP_332145.1
UniProt: Q3JWA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNALTLPNT LRPASAKAVS AGSLTLASHS MLPGHLGNID AYIQAVNRIP LLSAEEERQY ATEYREQNNL DAARRLVLSH LRLVVSVARN YLGYGLPHGD LIQEGNIGLM KAVKRFDPAQ NVRLVSYAIH WIKAEIHEYI LRNWRMVKVA TTKAQRKLFF NLRSHKKGTQ AFTPEEIDGL AQELNVKREE VAEMETRLSG GDIALEGQID DGEESYAPIA YLADSHNEPT AVLAARQRDM LQTDGIARAL ESLDARSRRI IEARWLNVDD DGSGGSTLHD LAAEFGVSAE RIRQIEASAM KKMRTALAAY A
NT Sequence
GTGAGCAACG CATTGACCCT TCCGAACACA CTGCGCCCGG CGTCCGCTAA GGCCGTATCG GCGGGCTCGC TGACGCTCGC CTCTCATTCG ATGCTGCCCG GCCATCTGGG CAACATCGAC GCCTATATCC AGGCTGTGAA CCGGATTCCG CTGCTAAGCG CGGAGGAAGA GCGTCAATAC GCGACCGAAT ACCGCGAGCA AAACAATCTC GACGCCGCGC GCCGGCTCGT GCTGTCGCAC CTGCGGCTCG TCGTGTCGGT CGCGCGCAAC TACCTCGGCT ACGGCCTGCC GCACGGCGAT CTGATCCAGG AAGGCAACAT CGGCCTGATG AAGGCGGTCA AGCGGTTCGA TCCCGCCCAG AACGTGCGCC TCGTGTCGTA CGCGATCCAC TGGATCAAGG CCGAGATTCA CGAGTACATC CTGCGCAACT GGCGCATGGT CAAGGTGGCG ACGACGAAGG CGCAGCGCAA GCTGTTCTTC AATCTGCGCA GCCACAAGAA GGGCACGCAG GCGTTCACGC CGGAGGAAAT CGACGGCCTC GCGCAGGAGC TGAACGTCAA GCGCGAGGAA GTGGCCGAGA TGGAAACCCG CCTGTCGGGC GGCGACATCG CGCTCGAAGG CCAGATCGAC GACGGCGAGG AATCGTACGC GCCGATCGCC TATCTCGCCG ATTCGCACAA CGAGCCGACC GCCGTGCTCG CCGCGCGGCA GCGCGACATG CTGCAGACGG ACGGCATCGC GCGCGCGCTC GAATCGCTCG ACGCACGCAG CCGCCGGATC ATCGAGGCGC GCTGGCTGAA CGTCGACGAC GACGGCTCGG GCGGCTCGAC GCTGCACGAT CTCGCGGCCG AATTCGGCGT GTCGGCGGAG CGCATCCGCC AGATCGAGGC AAGCGCGATG AAGAAGATGC GCACGGCGCT CGCCGCGTAC GCA
Details for BupsA.00367.b.A1.GE32540
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMVSNALT LPNTLRPASA KAVSAGSLTL ASHSMLPGHL GNIDAYIQAV NRIPLLSAEE ERQYATEYRE QNNLDAARRL VLSHLRLVVS VARNYLGYGL PHGDLIQEGN IGLMKAVKRF DPAQNVRLVS YAIHWIKAEI HEYILRNWRM VKVATTKAQR KLFFNLRSHK KGTQAFTPEE IDGLAQELNV KREEVAEMET RLSGGDIALE GQIDDGEESY APIAYLADSH NEPTAVLAAR QRDMLQTDGI ARALESLDAR SRRIIEARWL NVDDDGSGGS TLHDLAAEFG VSAERIRQIE ASAMKKMRTA LAA
Validated NT Sequence
acgcggcgag cgccgtgcgc atcttcttca tcgcgcttgc ctcgatctgg cggatgcgct ccgccgacac gccgaattcg gccgcgagat cgtgcagcgt cgagccgccc gagccgtcgt cgtcgacgtt cagccagcgc gcctcgatga tccggcggct gcgtgcgtcg agcgattcga gcgcgcgcgc gatgccgtcc gtctgcagca tgtcgcgctg ccgcgcggcg agcacggcgg tcggctcgtt gtgcgaatcg gcgagatagg cgatcggcgc gtacgattcc tcgccgtcgt cgatctggcc ttcgagcgcg atgtcgccgc ccgacaggcg ggtttccatc tcggccactt cctcgcgctt gacgttcagc tcctgcgcga ggccgtcgat ttcctccggc gtgaacgcct gcgtgccctt cttgtggctg cgcagattga agaacagctt gcgctgcgcc ttcgtcgtcg ccaccttgac catgcgccag ttgcgcagga tgtactcgtg aatctcggcc ttgatccagt ggatcgcgta cgacacgagg cgcacgttct gggcgggatc gaaccgcttg accgccttca tcaggccgat gttgccttcc tggatcagat cgccgtgcgg caggccgtag ccgaggtagt tgcgcgcgac cgacacgacg agccgcaggt gcgacagcac gagccggcgc gcggcgtcga gattgttttg ctcgcggtat tcggtcgcgt attgacgctc ttcctccgcg cttagcagcg gaatccggtt cacagcctgg atataggcgt cgatgttgcc cagatggccg ggcagcatcg aatgagaggc gagcgtcagc gagcccgccg atacggcctt agcggacgcc gggcgcagtg tgttcggaag ggtcaatgcg ttgctcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSNALTLP NTLRPASAKA VSAGSLTLAS HSMLPGHLGN IDAYIQAVNR IPLLSAEEER QYATEYREQN NLDAARRLVL SHLRLVVSVA RNYLGYGLPH GDLIQEGNIG LMKAVKRFDP AQNVRLVSYA IHWIKAEIHE YILRNWRMVK VATTKAQRKL FFNLRSHKKG TQAFTPEEID GLAQELNVKR EEVAEMETRL SGGDIALEGQ IDDGEESYAP IAYLADSHNE PTAVLAARQR DMLQTDGIAR ALESLDARSR RIIEARWLNV DDDGSGGSTL HDLAAEFGVS AERIRQIEAS AMKKMRTALA AYA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcaac gcattgaccc ttccgaacac actgcgcccg gcgtccgcta aggccgtatc ggcgggctcg ctgacgctcg cctctcattc gatgctgccc ggccatctgg gcaacatcga cgcctatatc caggctgtga accggattcc gctgctaagc gcggaggaag agcgtcaata cgcgaccgaa taccgcgagc aaaacaatct cgacgccgcg cgccggctcg tgctgtcgca cctgcggctc gtcgtgtcgg tcgcgcgcaa ctacctcggc tacggcctgc cgcacggcga tctgatccag gaaggcaaca tcggcctgat gaaggcggtc aagcggttcg atcccgccca gaacgtgcgc ctcgtgtcgt acgcgatcca ctggatcaag gccgagattc acgagtacat cctgcgcaac tggcgcatgg tcaaggtggc gacgacgaag gcgcagcgca agctgttctt caatctgcgc agccacaaga agggcacgca ggcgttcacg ccggaggaaa tcgacggcct cgcgcaggag ctgaacgtca agcgcgagga agtggccgag atggaaaccc gcctgtcggg cggcgacatc gcgctcgaag gccagatcga cgacggcgag gaatcgtacg cgccgatcgc ctatctcgcc gattcgcaca acgagccgac cgccgtgctc gccgcgcggc agcgcgacat gctgcagacg gacggcatcg cgcgcgcgct cgaatcgctc gacgcacgca gccgccggat catcgaggcg cgctggctga acgtcgacga cgacggctcg ggcggctcga cgctgcacga tctcgcggcc gaattcggcg tgtcggcgga gcgcatccgc cagatcgagg caagcgcgat gaagaagatg cgcacggcgc tcgccgcgta cgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa