SSGCID: Target Page

BupsA.00358.a

Enoyl-CoA hydratase/isomerase family protein domain protein

CENTER ID:	BupsA.00358.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00358.a.A1.GE32538	Full length( BupsA.00358.a )		1	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00358.a.A1.PS01373	Full length( BupsA.00358.a )		1	260

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.6836
RefSeq:	YP_337761.1
UniProt:	Q3JF92

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAHERVVEV REVADCVVQV VMQDRENKNT FSDALIRGLG EAFDAIRQNG RCKAAILTGY GNYFASGGTQ ESLMYLSEGK GTFAEIAASA SRNGANLYSL ALNCPVPVIA AMQGHGIGGG FVMGLFADFV VLGKECLYTT NFMKYGFTPG MGATLVLPHK LGLPLAQNML MTARSFYGDE LQKLGVPFPV VPRAEVAACA LEIAKNLAEK PRLSLVTLKD HLVAELRSRL PAVIEQELAM HEKTFGQPEV KERIRSLFGQ

NT Sequence

GTGAGCGCGC ATGAACGAGT CGTCGAAGTC CGCGAGGTAG CGGACTGCGT AGTCCAGGTC GTCATGCAGG ATCGCGAGAA CAAGAACACG TTCTCCGACG CGCTGATCCG GGGGCTCGGC GAGGCATTCG ACGCGATCCG CCAGAACGGC CGGTGCAAGG CCGCGATCCT CACCGGCTAC GGCAACTATT TCGCGAGCGG CGGCACGCAG GAAAGCCTCA TGTACCTGTC CGAGGGCAAG GGCACGTTCG CGGAGATCGC CGCGAGCGCG AGCCGCAACG GCGCGAACCT GTACAGCCTC GCGCTGAACT GCCCCGTCCC CGTCATTGCG GCGATGCAGG GGCACGGCAT CGGCGGCGGC TTCGTGATGG GCCTGTTCGC GGACTTCGTC GTGCTCGGCA AGGAATGCCT GTACACCACC AATTTCATGA AGTACGGCTT CACGCCGGGC ATGGGCGCGA CGCTCGTGCT GCCGCACAAG CTCGGCCTGC CGCTCGCGCA GAACATGCTG ATGACCGCCC GTTCGTTCTA CGGCGACGAG CTGCAAAAGC TCGGCGTGCC GTTTCCGGTG GTGCCGCGCG CGGAAGTGGC CGCGTGTGCG CTCGAGATCG CGAAGAATCT CGCCGAAAAG CCGCGGCTGT CGCTCGTCAC GCTGAAGGAT CATCTGGTGG CCGAGCTGCG CTCGAGACTG CCGGCCGTGA TCGAGCAGGA GCTCGCGATG CATGAAAAGA CCTTCGGCCA GCCCGAGGTC AAGGAGCGCA TCCGCTCGCT CTTCGGACAA

Details for BupsA.00358.a.A1.GE32538

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

MVSAHERVVE VREVADCVVQ VVMQDRENKN TFSDALIRGL GEAFDAIRQN GRCKAAILTG YGNYFASGGT QESLMYLSEG KGTFAEIAAS ASRNGANLYS LALNCPVPVI AAMQGHGIGG GFVMGLFADF VVLGKECLYT TNFMKYGFTP GMGATLVLPH KLGLPLAQNM LMTARSFYGD ELQKLGVPFP VVPRAEVAAC ALEIAKNLAE KPRLSLVTLK DHLVAELRSR LPAVIEQELA MHEKTFGQPE VKERIRSLFG Q

Validated NT Sequence

ttattattgt ccgaagagcg agcggatgcg ctccttgacc tcgggctggc cgaaggtctt ttcatgcatc gcgagctcct gctcgatcac ggccggcagt ctcgagcgca gctcggccac cagatgatcc ttcagcgtga cgagcgacag ccgcggcttt tcggcgagat tcttcgcgat ctcgagcgca cacgcggcca cttccgcgcg cggcaccacc ggaaacggca cgccgagctt ttgcagctcg tcgccgtaga acgaacgggc ggtcatcagc atgttctgcg cgagcggcag gccgagcttg tgcggcagca cgagcgtcgc gcccatgccc ggcgtgaagc cgtacttcat gaaattggtg gtgtacaggc attccttgcc gagcacgacg aagtccgcga acaggcccat cacgaagccg ccgccgatgc cgtgcccctg catcgccgca atgacgggga cggggcagtt cagcgcgagg ctgtacaggt tcgcgccgtt gcggctcgcg ctcgcggcga tctccgcgaa cgtgcccttg ccctcggaca ggtacatgag gctttcctgc gtgccgccgc tcgcgaaata gttgccgtag ccggtgagga tcgcggcctt gcaccggccg ttctggcgga tcgcgtcgaa tgcctcgccg agcccccgga tcagcgcgtc ggagaacgtg ttcttgttct cgcgatcctg catgacgacc tggactacgc agtccgctac ctcgcggact tcgacgactc gttcatgcgc gctcaccatc gaaccaggac cctgggtctg agcttccag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSAHERVV EVREVADCVV QVVMQDRENK NTFSDALIRG LGEAFDAIRQ NGRCKAAILT GYGNYFASGG TQESLMYLSE GKGTFAEIAA SASRNGANLY SLALNCPVPV IAAMQGHGIG GGFVMGLFAD FVVLGKECLY TTNFMKYGFT PGMGATLVLP HKLGLPLAQN MLMTARSFYG DELQKLGVPF PVVPRAEVAA CALEIAKNLA EKPRLSLVTL KDHLVAELRS RLPAVIEQEL AMHEKTFGQP EVKERIRSLF GQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgcg catgaacgag tcgtcgaagt ccgcgaggta gcggactgcg tagtccaggt cgtcatgcag gatcgcgaga acaagaacac gttctccgac gcgctgatcc gggggctcgg cgaggcattc gacgcgatcc gccagaacgg ccggtgcaag gccgcgatcc tcaccggcta cggcaactat ttcgcgagcg gcggcacgca ggaaagcctc atgtacctgt ccgagggcaa gggcacgttc gcggagatcg ccgcgagcgc gagccgcaac ggcgcgaacc tgtacagcct cgcgctgaac tgccccgtcc ccgtcattgc ggcgatgcag gggcacggca tcggcggcgg cttcgtgatg ggcctgttcg cggacttcgt cgtgctcggc aaggaatgcc tgtacaccac caatttcatg aagtacggct tcacgccggg catgggcgcg acgctcgtgc tgccgcacaa gctcggcctg ccgctcgcgc agaacatgct gatgaccgcc cgttcgttct acggcgacga gctgcaaaag ctcggcgtgc cgtttccggt ggtgccgcgc gcggaagtgg ccgcgtgtgc gctcgagatc gcgaagaatc tcgccgaaaa gccgcggctg tcgctcgtca cgctgaagga tcatctggtg gccgagctgc gctcgagact gccggccgtg atcgagcagg agctcgcgat gcatgaaaag accttcggcc agcccgaggt caaggagcgc atccgctcgc tcttcggaca ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BupsA.00358.a.A1.PS01373

PURIFICATION DATe:	1/31/2012
CONCENTRATION:	32.73mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCGCG CATGAACGAG TCGTCGAAGT CCGCGAGGTA GCGGACTGCG TAGTCCAGGT CGTCATGCAG GATCGCGAGA ACAAGAACAC GTTCTCCGAC GCGCTGATCC GGGGGCTCGG CGAGGCATTC GACGCGATCC GCCAGAACGG CCGGTGCAAG GCCGCGATCC TCACCGGCTA CGGCAACTAT TTCGCGAGCG GCGGCACGCA GGAAAGCCTC ATGTACCTGT CCGAGGGCAA GGGCACGTTC GCGGAGATCG CCGCGAGCGC GAGCCGCAAC GGCGCGAACC TGTACAGCCT CGCGCTGAAC TGCCCCGTCC CCGTCATTGC GGCGATGCAG GGGCACGGCA TCGGCGGCGG CTTCGTGATG GGCCTGTTCG CGGACTTCGT CGTGCTCGGC AAGGAATGCC TGTACACCAC CAATTTCATG AAGTACGGCT TCACGCCGGG CATGGGCGCG ACGCTCGTGC TGCCGCACAA GCTCGGCCTG CCGCTCGCGC AGAACATGCT GATGACCGCC CGTTCGTTCT ACGGCGACGA GCTGCAAAAG CTCGGCGTGC CGTTTCCGGT GGTGCCGCGC GCGGAAGTGG CCGCGTGTGC GCTCGAGATC GCGAAGAATC TCGCCGAAAA GCCGCGGCTG TCGCTCGTCA CGCTGAAGGA TCATCTGGTG GCCGAGCTGC GCTCGAGACT GCCGGCCGTG ATCGAGCAGG AGCTCGCGAT GCATGAAAAG ACCTTCGGCC AGCCCGAGGT CAAGGAGCGC ATCCGCTCGC TCTTCGGACA ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA