BupsA.00339.b

Alanine racemase

CENTER ID: BupsA.00339.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00339.b.A1.GE32537 Full length( BupsA.00339.b ) 1 374
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.6468
RefSeq: YP_337437.1
UniProt: Q3JG66

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRPIVARIH PDAVAHNLAI VRQKAPNARI WSVVKANAYG HGIEHVYPAL AGADGIALLD LDEAVRVREL GWTKPVLLLE GIFEAADVEI ADRHRLTVAV HGDEQFKLLT AAKTTRPIDI QLKMNSGMNR LGYRPHALRA AWERAAAAPS IGRIALMMHF ANADEGEIDW QMSVFDAATQ GLPGERTLSN SAAVLWHPRA HRDWVRPGTI LYGASPTGAS RHVADVPLRA AMTVTSRIIG VQTLTPGETV GYGRCFAAER EMRIGVVACG YADGYPRHAP TGTPIAVDGV RTQVVGRVSM DMLTVDLTPC PNAGVGSAVE LWGEQVRVDD VAEAAGTIGY ELMCALARRV PVTVDPSPAA AALAQPVRTG SYGR
NT Sequence
ATGCCTCGCC CCATCGTCGC CCGCATTCAT CCCGACGCCG TCGCGCACAA TCTTGCAATC GTCCGGCAAA AGGCGCCGAA CGCGCGTATC TGGTCGGTCG TCAAGGCGAA CGCGTACGGC CACGGCATCG AGCATGTCTA TCCGGCGCTC GCCGGCGCGG ACGGCATCGC GCTGCTCGAC CTCGACGAAG CGGTGCGCGT GCGCGAGCTC GGCTGGACGA AGCCCGTGCT GCTGCTCGAA GGCATCTTCG AGGCGGCCGA TGTCGAGATC GCGGATCGCC ATCGGCTGAC GGTCGCCGTG CACGGCGACG AGCAGTTCAA GCTGCTCACC GCCGCGAAGA CGACGCGCCC GATCGACATC CAACTGAAGA TGAACTCCGG GATGAACCGG CTCGGCTACC GGCCGCACGC GTTGCGCGCC GCGTGGGAAC GCGCGGCCGC CGCGCCGTCG ATCGGCCGCA TCGCGCTGAT GATGCATTTC GCGAACGCCG ACGAAGGCGA GATCGACTGG CAGATGAGCG TGTTCGACGC GGCGACGCAG GGCCTGCCCG GCGAGCGCAC GCTGTCGAAC TCGGCCGCCG TGCTGTGGCA TCCGCGCGCG CACCGCGACT GGGTGCGCCC CGGCACGATC CTGTACGGCG CGTCGCCCAC GGGCGCGAGC CGCCACGTCG CCGACGTACC GCTGCGGGCC GCGATGACGG TCACGAGCCG GATCATCGGC GTGCAAACGC TGACGCCGGG CGAGACGGTC GGCTACGGGC GCTGCTTCGC GGCCGAGCGC GAGATGCGCA TCGGCGTCGT CGCCTGCGGC TACGCGGACG GCTATCCGCG CCACGCGCCC ACCGGCACGC CGATCGCGGT CGACGGCGTG CGCACGCAAG TGGTCGGGCG CGTGTCGATG GACATGCTGA CCGTCGACCT CACGCCGTGT CCGAACGCGG GCGTCGGCTC GGCCGTCGAG CTGTGGGGCG AGCAGGTGCG CGTCGACGAC GTCGCCGAAG CCGCGGGCAC GATCGGCTAC GAGCTGATGT GCGCGCTCGC GCGGCGCGTG CCGGTCACCG TCGATCCGTC GCCCGCCGCG GCCGCGCTCG CCCAGCCGGT GCGCACCGGA AGCTACGGGC GC
Details for BupsA.00339.b.A1.GE32537
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: data unavailable
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPRPIVARI HPDAVAHNLA IVRQKAPNAR IWSVVKANAY GHGIEHVYPA LAGADGIALL DLDEAVRVRE LGWTKPVLLL EGIFEAADVE IADRHRLTVA VHGDEQFKLL TAAKTTRPID IQLKMNSGMN RLGYRPHALR AAWERAAAAP SIGRIALMMH FANADEGEID WQMSVFDAAT QGLPGERTLS NSAAVLWHPR AHRDWVRPGT ILYGASPTGA SRHVADVPLR AAMTVTSRII GVQTLTPGET VGYGRCFAAE REMRIGVVAC GYAXGYPRHA PTGTPIAVXX VRTQVXRVSM DXLTVXXTXX PXGVXSXXXL XGXXXAXXXX XXXXXXXLXX XXXXGXXXXX XXXXXPXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttntttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcctcgc cccatcgtcg cccgcattca tcccgacgcc gtcgcgcaca atcttgcaat cgtccggcaa aaggcgccga acgcgcgtat ctggtcggtc gtcaaggcga acgcgtacgg ccacggcatc gagcatgtct atccggcgct cgccggcgcg gacggcatcg cgctgctcga cctcgacgaa gcggtgcgcg tgcgcgagct cggctggacg aagcccgtgc tgctgctcga aggcatcttc gaggcggccg atgtcgagat cgcggatcgc catcggctga cggtcgccgt gcacggcgac gagcagttca agctgctcac cgccgcgaag acgacgcgcc cgatcgacat ccaactgaag atgaactccg ggatgaaccg gctcggctac cggccgcacg cgttgcgcgc cgcgtgggaa cgcgcggccg ccgcgccgtc gatcggccgc atcgcgctga tgatgcattt cgcgaacgcc gacgaaggcg agatcgactg gcagatgagc gtgttcgacg cggcgacgca gggcctgccc ggcgagcgca cgctgtcgaa ctcggccgcc gtgctgtggc atccgcgcgc gcaccgcgac tgggtgcgcc ccggcacgat cctgtacggc gcgtcgccca cgggcgcgag ccgccacgtc gccgacgtac cgctgcgggc cgcgatgacg gtcacgagcc ggatcatcgg cgtgcaaacg ctgacgccgg gcgagacggt cggctacggg cgctgcttcg cggccgagcg cgagatgcgc atcggcgtcg tcgcctgcgg ctacgcggan ggctatccnc gccacgcgcc caccggcacg ccgatcgcgg tcgangnngt gcgcacgcaa gtngnncgcg tgtcgatgga cntgctgacc gtcnannnca cgncnnntcc gannggcgtn nnntcnnnng ncnngctgnn gggnnnnanc nnngcnnnnc nnnnnnnnnc nannnnnngg nnnnatnngc tannnnnnnn gnnnnnnnng ggnnnnnnnc nnnntnnnnn nnnnnnnnnt nnnccnnncn nggnnnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnn nnnnngnnnn nnnnnannnn nnnnnnnnna ana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPRPIVARI HPDAVAHNLA IVRQKAPNAR IWSVVKANAY GHGIEHVYPA LAGADGIALL DLDEAVRVRE LGWTKPVLLL EGIFEAADVE IADRHRLTVA VHGDEQFKLL TAAKTTRPID IQLKMNSGMN RLGYRPHALR AAWERAAAAP SIGRIALMMH FANADEGEID WQMSVFDAAT QGLPGERTLS NSAAVLWHPR AHRDWVRPGT ILYGASPTGA SRHVADVPLR AAMTVTSRII GVQTLTPGET VGYGRCFAAE REMRIGVVAC GYADGYPRHA PTGTPIAVDG VRTQVVGRVS MDMLTVDLTP CPNAGVGSAV ELWGEQVRVD DVAEAAGTIG YELMCALARR VPVTVDPSPA AAALAQPVRT GSYGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctcgcccc atcgtcgccc gcattcatcc cgacgccgtc gcgcacaatc ttgcaatcgt ccggcaaaag gcgccgaacg cgcgtatctg gtcggtcgtc aaggcgaacg cgtacggcca cggcatcgag catgtctatc cggcgctcgc cggcgcggac ggcatcgcgc tgctcgacct cgacgaagcg gtgcgcgtgc gcgagctcgg ctggacgaag cccgtgctgc tgctcgaagg catcttcgag gcggccgatg tcgagatcgc ggatcgccat cggctgacgg tcgccgtgca cggcgacgag cagttcaagc tgctcaccgc cgcgaagacg acgcgcccga tcgacatcca actgaagatg aactccggga tgaaccggct cggctaccgg ccgcacgcgt tgcgcgccgc gtgggaacgc gcggccgccg cgccgtcgat cggccgcatc gcgctgatga tgcatttcgc gaacgccgac gaaggcgaga tcgactggca gatgagcgtg ttcgacgcgg cgacgcaggg cctgcccggc gagcgcacgc tgtcgaactc ggccgccgtg ctgtggcatc cgcgcgcgca ccgcgactgg gtgcgccccg gcacgatcct gtacggcgcg tcgcccacgg gcgcgagccg ccacgtcgcc gacgtaccgc tgcgggccgc gatgacggtc acgagccgga tcatcggcgt gcaaacgctg acgccgggcg agacggtcgg ctacgggcgc tgcttcgcgg ccgagcgcga gatgcgcatc ggcgtcgtcg cctgcggcta cgcggacggc tatccgcgcc acgcgcccac cggcacgccg atcgcggtcg acggcgtgcg cacgcaagtg gtcgggcgcg tgtcgatgga catgctgacc gtcgacctca cgccgtgtcc gaacgcgggc gtcggctcgg ccgtcgagct gtggggcgag caggtgcgcg tcgacgacgt cgccgaagcc gcgggcacga tcggctacga gctgatgtgc gcgctcgcgc ggcgcgtgcc ggtcaccgtc gatccgtcgc ccgccgcggc cgcgctcgcc cagccggtgc gcaccggaag ctacgggcgc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa