SSGCID: Target Page

BupsA.00231.a

Threonine dehydratase catabolic

CENTER ID:	BupsA.00231.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00231.a.A1.GE32535	Full length( BupsA.00231.a )		1	343

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00231.a.A1.PS01415	Full length( BupsA.00231.a )		1	343

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.2632
RefSeq:	YP_333907.1
UniProt:	Q3JR96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTSPNRPAH LTINGEPIPT LDEIAAQHFA LTPWVMRTPV FERADLPSLD NTHVNFKFEL LQASGSFKAR GAFTNLLALG EDRKSVGVTC VSAGNHAAAV AYAAMRLGTS AKVVMFDTAS PARVALCRHY RAEVVMASSP AEAFEIVRRI EAEEGRFFVH PFNGYHTILG TATLGYEWAT QTPDLDAVIV PIGGGGLAAG TSTALRLANP RVHVYGVEPE GADAMSRSFA ANHPVRMGAM HTIADSLMAP HTEEYSYELC RRHVDRIVTV SDDALRAAML YLFAHLKVAV EPACAAATAA LLGPLRESLQ GRRVGVLLSG TNIDSTALAA HLEHARLHRA AQP

NT Sequence

ATGTCGACCT CACCCAACCG CCCCGCTCAT CTGACGATCA ACGGCGAGCC GATCCCGACG CTCGACGAGA TCGCCGCGCA ACACTTCGCG CTCACGCCGT GGGTCATGCG CACGCCGGTG TTCGAGCGCG CGGATCTGCC GTCGCTCGAC AACACGCACG TGAACTTCAA GTTCGAGCTG CTGCAGGCGA GCGGCAGCTT CAAGGCGCGC GGCGCGTTCA CGAACCTGCT CGCGCTCGGC GAGGACAGGA AGAGCGTGGG CGTCACCTGC GTGTCGGCCG GCAATCACGC GGCGGCCGTC GCGTATGCGG CGATGCGACT CGGCACGAGC GCGAAGGTCG TGATGTTCGA CACCGCGAGC CCGGCGCGCG TCGCGCTCTG CCGCCACTAC CGCGCGGAGG TCGTGATGGC GTCGAGCCCC GCCGAGGCGT TCGAGATCGT GCGGCGCATC GAAGCCGAGG AAGGCCGTTT CTTCGTGCAT CCGTTCAACG GCTACCACAC GATTCTCGGC ACCGCGACGC TCGGCTACGA ATGGGCGACG CAGACGCCCG ACCTCGATGC GGTGATCGTG CCGATCGGCG GGGGCGGCCT CGCCGCGGGC ACGTCGACCG CGCTGCGCCT CGCGAACCCG CGCGTGCACG TGTACGGCGT CGAGCCGGAA GGCGCGGATG CGATGAGCCG CAGCTTCGCC GCGAATCACC CGGTCCGCAT GGGCGCGATG CACACGATCG CCGATTCGCT GATGGCGCCG CACACCGAGG AGTACAGCTA CGAGCTGTGT CGTCGCCACG TCGACCGGAT CGTCACCGTG TCGGACGACG CGCTGCGCGC GGCGATGCTC TATCTGTTCG CGCATCTGAA GGTCGCGGTC GAGCCCGCGT GCGCGGCCGC GACGGCCGCC CTCCTCGGGC CGCTGCGCGA GTCGCTGCAG GGCAGGCGCG TCGGCGTGCT GCTGTCGGGC ACCAACATCG ATTCGACCGC GCTCGCCGCG CATCTCGAGC ACGCGCGGCT GCATCGGGCC GCGCAACCG

Details for BupsA.00231.a.A1.GE32535

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSTSPNRPA HLTINGEPIP TLDEIAAQHF ALTPWVMRTP VFERADLPSL DNTHVNFKFE LLQASGSFKA RGAFTNLLAL GEDRKSVGVT CVSAGNHAAA VAYAAMRLGT SAKVVMFDTA SPARVALCRH YRAEVVMASS PAEAFEIVRR IEAEEGRFFV HPFNGYHTIL GTATLGYEWA TQTPDLDAVI VPIGGGGLAA GTSTALRLAN PRVHVYGVEP EGADAMSRSF AANHPVRMGA MHTIADSLMA PHTEEYSYEL CRRHVDRIVT VSDDALRAAM LYLFAHLKVA VEPACAAATA ALLGPLRESL QGRRVGVLLS GTNIDSTALA AHLEHA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcga cctcacccaa ccgccccgct catctgacga tcaacggcga gccgatcccg acgctcgacg agatcgccgc gcaacacttc gcgctcacgc cgtgggtcat gcgcacgccg gtgttcgagc gcgcggatct gccgtcgctc gacaacacgc acgtgaactt caagttcgag ctgctgcagg cgagcggcag cttcaaggcg cgcggcgcgt tcacgaacct gctcgcgctc ggcgaggaca ggaagagcgt gggcgtcacc tgcgtgtcgg ccggcaatca cgcggcggcc gtcgcgtatg cggcgatgcg actcggcacg agcgcgaagg tcgtgatgtt cgacaccgcg agcccggcgc gcgtcgcgct ctgccgccac taccgcgcgg aggtcgtgat ggcgtcgagc cccgccgagg cgttcgagat cgtgcggcgc atcgaagccg aggaaggccg tttcttcgtg catccgttca acggctacca cacgattctc ggcaccgcga cgctcggcta cgaatgggcg acgcagacgc ccgacctcga tgcggtgatc gtgccgatcg gcgggggcgg cctcgccgcg ggcacgtcga ccgcgctgcg cctcgcgaac ccgcgcgtgc acgtgtacgg cgtcgagccg gaaggcgcgg atgcgatgag ccgcagcttc gccgcgaatc acccggtccg catgggcgcg atgcacacga tcgccgattc gctgatggcg ccgcacaccg aggagtacag ctacgagctg tgtcgtcgcc acgtcgaccg gatcgtcacc gtgtcggacg acgcgctgcg cgcggcgatg ctctatctgt tcgcgcatct gaaggtcgcg gtcgagcccg cgtgcgcggc cgcgacggcc gccctcctcg ggccgctgcg cgagtcgctg cagggcaggc gcgtcggcgt gctgctgtcg ggcaccaaca tcgattcgac cgcgctcgcc gcgcatctcg agcacgcgcg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgacctca cccaaccgcc ccgctcatct gacgatcaac ggcgagccga tcccgacgct cgacgagatc gccgcgcaac acttcgcgct cacgccgtgg gtcatgcgca cgccggtgtt cgagcgcgcg gatctgccgt cgctcgacaa cacgcacgtg aacttcaagt tcgagctgct gcaggcgagc ggcagcttca aggcgcgcgg cgcgttcacg aacctgctcg cgctcggcga ggacaggaag agcgtgggcg tcacctgcgt gtcggccggc aatcacgcgg cggccgtcgc gtatgcggcg atgcgactcg gcacgagcgc gaaggtcgtg atgttcgaca ccgcgagccc ggcgcgcgtc gcgctctgcc gccactaccg cgcggaggtc gtgatggcgt cgagccccgc cgaggcgttc gagatcgtgc ggcgcatcga agccgaggaa ggccgtttct tcgtgcatcc gttcaacggc taccacacga ttctcggcac cgcgacgctc ggctacgaat gggcgacgca gacgcccgac ctcgatgcgg tgatcgtgcc gatcggcggg ggcggcctcg ccgcgggcac gtcgaccgcg ctgcgcctcg cgaacccgcg cgtgcacgtg tacggcgtcg agccggaagg cgcggatgcg atgagccgca gcttcgccgc gaatcacccg gtccgcatgg gcgcgatgca cacgatcgcc gattcgctga tggcgccgca caccgaggag tacagctacg agctgtgtcg tcgccacgtc gaccggatcg tcaccgtgtc ggacgacgcg ctgcgcgcgg cgatgctcta tctgttcgcg catctgaagg tcgcggtcga gcccgcgtgc gcggccgcga cggccgccct cctcgggccg ctgcgcgagt cgctgcaggg caggcgcgtc ggcgtgctgc tgtcgggcac caacatcgat tcgaccgcgc tcgccgcgca tctcgagcac gcgcggctgc atcgggccgc gcaaccgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BupsA.00231.a.A1.PS01415

PURIFICATION DATe:	3/10/2012
CONCENTRATION:	12.01mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGACCTCA CCCAACCGCC CCGCTCATCT GACGATCAAC GGCGAGCCGA TCCCGACGCT CGACGAGATC GCCGCGCAAC ACTTCGCGCT CACGCCGTGG GTCATGCGCA CGCCGGTGTT CGAGCGCGCG GATCTGCCGT CGCTCGACAA CACGCACGTG AACTTCAAGT TCGAGCTGCT GCAGGCGAGC GGCAGCTTCA AGGCGCGCGG CGCGTTCACG AACCTGCTCG CGCTCGGCGA GGACAGGAAG AGCGTGGGCG TCACCTGCGT GTCGGCCGGC AATCACGCGG CGGCCGTCGC GTATGCGGCG ATGCGACTCG GCACGAGCGC GAAGGTCGTG ATGTTCGACA CCGCGAGCCC GGCGCGCGTC GCGCTCTGCC GCCACTACCG CGCGGAGGTC GTGATGGCGT CGAGCCCCGC CGAGGCGTTC GAGATCGTGC GGCGCATCGA AGCCGAGGAA GGCCGTTTCT TCGTGCATCC GTTCAACGGC TACCACACGA TTCTCGGCAC CGCGACGCTC GGCTACGAAT GGGCGACGCA GACGCCCGAC CTCGATGCGG TGATCGTGCC GATCGGCGGG GGCGGCCTCG CCGCGGGCAC GTCGACCGCG CTGCGCCTCG CGAACCCGCG CGTGCACGTG TACGGCGTCG AGCCGGAAGG CGCGGATGCG ATGAGCCGCA GCTTCGCCGC GAATCACCCG GTCCGCATGG GCGCGATGCA CACGATCGCC GATTCGCTGA TGGCGCCGCA CACCGAGGAG TACAGCTACG AGCTGTGTCG TCGCCACGTC GACCGGATCG TCACCGTGTC GGACGACGCG CTGCGCGCGG CGATGCTCTA TCTGTTCGCG CATCTGAAGG TCGCGGTCGA GCCCGCGTGC GCGGCCGCGA CGGCCGCCCT CCTCGGGCCG CTGCGCGAGT CGCTGCAGGG CAGGCGCGTC GGCGTGCTGC TGTCGGGCAC CAACATCGAT TCGACCGCGC TCGCCGCGCA TCTCGAGCAC GCGCGGCTGC ATCGGGCCGC GCAACCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA