BupsA.00190.a

L-allo-threonine aldolase

CENTER ID: BupsA.00190.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00190.a.A1.GE32534 Full length( BupsA.00190.a ) 1 397
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00190.a.A1.PW32454 Full length( BupsA.00190.a ) 1 397

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.5914
RefSeq: YP_336928.1
UniProt: Q3JHM5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPRTAHRIG ARAPRRARAA SAASSGATPN CVHSKPPLHR ASAPAHRAIL LFDETPTGTQ MIDLRSDTVT RPSAPMLAAM TAAEVGDDVW GDDPTVARLQ AVVAERAGKE AALFFPSGTQ SNLAALMSHC ERGDEYIVGQ AAHTYKYEGG GAAVLGSIQP QPIENAADGT LPLEKIAAAI KPIDDHFARS RLFTLENTIG GKVLPAGYVD EAVALARSRG LAAHLDGARV CNAAVASGRA IAELCAPFDS VSICFSKGLG APVGSVLVGS GALIERAHRW RKVLGGGMRQ AGVLAAACLY ALEHNVERLA DDHANAERLA QGLARIEPVK VLSQATNMVF AQFPEADCAP LEAWLKERGI LTQMLYASRF VTHCDVSRDD IDTAVAAISA YFARSRA
NT Sequence
ATGCGCCCGC GAACGGCTCA CCGTATCGGC GCGCGAGCGC CGCGTCGCGC CCGCGCCGCT TCGGCCGCGT CAAGCGGCGC GACGCCGAAT TGCGTACACT CGAAGCCTCC GCTTCACCGC GCGAGCGCGC CGGCGCACCG CGCAATCCTC CTCTTCGACG AGACTCCGAC GGGAACCCAG ATGATCGATT TACGCAGCGA TACCGTGACG CGCCCGAGCG CGCCGATGCT GGCCGCGATG ACGGCCGCCG AAGTGGGCGA CGACGTGTGG GGCGACGATC CCACCGTCGC GCGCCTGCAG GCCGTGGTGG CCGAGCGCGC GGGCAAGGAG GCGGCGCTGT TCTTTCCGAG CGGCACGCAG AGCAACCTCG CGGCGCTGAT GTCGCACTGC GAGCGCGGCG ACGAGTACAT CGTCGGCCAG GCCGCGCACA CGTACAAATA CGAGGGCGGC GGCGCGGCGG TGCTCGGCAG CATTCAGCCG CAGCCGATCG AGAACGCCGC GGACGGCACG CTGCCGCTCG AGAAGATCGC GGCCGCGATC AAGCCGATCG ACGATCACTT CGCCCGCTCG CGCCTGTTCA CGCTTGAGAA CACGATCGGC GGCAAGGTGC TGCCGGCGGG CTATGTCGAC GAAGCGGTCG CGCTCGCGCG CAGCCGCGGG CTCGCTGCGC ATCTCGACGG CGCGCGCGTA TGCAACGCGG CCGTCGCGTC GGGCCGCGCG ATCGCCGAGC TGTGCGCGCC GTTCGATTCG GTGTCGATCT GCTTTTCGAA GGGGCTCGGC GCGCCGGTGG GCTCGGTGCT CGTCGGCAGC GGCGCGCTGA TCGAGCGCGC GCACCGCTGG CGCAAGGTGC TCGGCGGCGG CATGCGCCAG GCGGGCGTGC TCGCGGCCGC GTGCCTGTAT GCGCTCGAGC ACAACGTCGA GCGTCTCGCC GACGATCACG CGAACGCCGA GCGCCTCGCG CAGGGCCTCG CGCGCATCGA GCCGGTGAAG GTGCTGTCGC AGGCGACGAA CATGGTGTTC GCGCAATTTC CCGAAGCCGA TTGCGCGCCG CTCGAAGCAT GGCTCAAGGA GCGCGGGATT CTCACGCAGA TGCTGTATGC GTCGCGCTTC GTCACGCACT GCGACGTGTC GCGCGACGAC ATCGACACGG CTGTCGCCGC GATCAGCGCG TATTTCGCGC GTTCGCGCGC T
Details for BupsA.00190.a.A1.GE32534
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRPRTAHRI GARAPRRARA ASAASSGATP NCVHSKPPLH RASAPAHRAI LLFDETPTGT QMIDLRSDTV TRPSAPMLAA MTAAEVGDDV WGDDPTVARL QAVVAERAGK EAALFFPSGT QSNLAALMSH CERGDEYIVG QAAHTYKYEG GGAAVLGSIQ PQPIENAADG TLPLEKIAAA IKPIDDHFAR SRLFTLENTI GGKVLPAGYV DEAVALARSR GLAAHLDGAR VCNAAVASGR AIAELCAPFD SVSICFSKGL GAPVGSVLVG SGALIERAHR WRKVLGGGMR QAGVLAAACL YALEHNVERL ADDHANAERL AQGLARIEPV KVLSQATNMV FAQFPEADCA PLEAWLKERG ILTQMLYASR FVTHCDVSRD DIDTAVAAIS AYFARSRA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcc cgcgaacggc tcaccgtatc ggcgcgcgag cgccgcgtcg cgcccgcgcc gcttcggccg cgtcaagcgg cgcgacgccg aattgcgtac actcgaagcc tccgcttcac cgcgcgagcg cgccggcgca ccgcgcaatc ctcctcttcg acgagactcc gacgggaacc cagatgatcg atttacgcag cgataccgtg acgcgcccga gcgcgccgat gctggccgcg atgacggccg ccgaagtggg cgacgacgtg tggggcgacg atcccaccgt cgcgcgcctg caggccgtgg tggccgagcg cgcgggcaag gaggcggcgc tgttctttcc gagcggcacg cagagcaacc tcgcggcgct gatgtcgcac tgcgagcgcg gcgacgagta catcgtcggc caggccgcgc acacgtacaa atacgagggc ggcggcgcgg cggtgctcgg cagcattcag ccgcagccga tcgagaacgc cgcggacggc acgctgccgc tcgagaagat cgcggccgcg atcaagccga tcgacgatca cttcgcccgc tcgcgcctgt tcacgcttga gaacacgatc ggcggcaagg tgctgccggc gggctatgtc gacgaagcgg tcgcgctcgc gcgcagccgc gggctcgctg cgcatctcga cggcgcgcgc gtatgcaacg cggccgtcgc gtcgggccgc gcgatcgccg agctgtgcgc gccgttcgat tcggtgtcga tctgcttttc gaaggggctc ggcgcgccgg tgggctcggt gctcgtcggc agcggcgcgc tgatcgagcg cgcgcaccgc tggcgcaagg tgctcggcgg cggcatgcgc caggcgggcg tgctcgcggc cgcgtgcctg tatgcgctcg agcacaacgt cgagcgtctc gccgacgatc acgcgaacgc cgagcgcctc gcgcagggcc tcgcgcgcat cgagccggtg aaggtgctgt cgcaggcgac gaacatggtg ttcgcgcaat ttcccgaagc cgattgcgcg ccgctcgaag catggctcaa ggagcgcggg attctcacgc agatgctgta tgcgtcgcgc ttcgtcacgc actgcgacgt gtcgcgcgac gacatcgaca cggctgtcgc cgcgatcagc gcgtatttcg cgcgttcgcg cgctt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPRTAHRI GARAPRRARA ASAASSGATP NCVHSKPPLH RASAPAHRAI LLFDETPTGT QMIDLRSDTV TRPSAPMLAA MTAAEVGDDV WGDDPTVARL QAVVAERAGK EAALFFPSGT QSNLAALMSH CERGDEYIVG QAAHTYKYEG GGAAVLGSIQ PQPIENAADG TLPLEKIAAA IKPIDDHFAR SRLFTLENTI GGKVLPAGYV DEAVALARSR GLAAHLDGAR VCNAAVASGR AIAELCAPFD SVSICFSKGL GAPVGSVLVG SGALIERAHR WRKVLGGGMR QAGVLAAACL YALEHNVERL ADDHANAERL AQGLARIEPV KVLSQATNMV FAQFPEADCA PLEAWLKERG ILTQMLYASR FVTHCDVSRD DIDTAVAAIS AYFARSRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcccgcga acggctcacc gtatcggcgc gcgagcgccg cgtcgcgccc gcgccgcttc ggccgcgtca agcggcgcga cgccgaattg cgtacactcg aagcctccgc ttcaccgcgc gagcgcgccg gcgcaccgcg caatcctcct cttcgacgag actccgacgg gaacccagat gatcgattta cgcagcgata ccgtgacgcg cccgagcgcg ccgatgctgg ccgcgatgac ggccgccgaa gtgggcgacg acgtgtgggg cgacgatccc accgtcgcgc gcctgcaggc cgtggtggcc gagcgcgcgg gcaaggaggc ggcgctgttc tttccgagcg gcacgcagag caacctcgcg gcgctgatgt cgcactgcga gcgcggcgac gagtacatcg tcggccaggc cgcgcacacg tacaaatacg agggcggcgg cgcggcggtg ctcggcagca ttcagccgca gccgatcgag aacgccgcgg acggcacgct gccgctcgag aagatcgcgg ccgcgatcaa gccgatcgac gatcacttcg cccgctcgcg cctgttcacg cttgagaaca cgatcggcgg caaggtgctg ccggcgggct atgtcgacga agcggtcgcg ctcgcgcgca gccgcgggct cgctgcgcat ctcgacggcg cgcgcgtatg caacgcggcc gtcgcgtcgg gccgcgcgat cgccgagctg tgcgcgccgt tcgattcggt gtcgatctgc ttttcgaagg ggctcggcgc gccggtgggc tcggtgctcg tcggcagcgg cgcgctgatc gagcgcgcgc accgctggcg caaggtgctc ggcggcggca tgcgccaggc gggcgtgctc gcggccgcgt gcctgtatgc gctcgagcac aacgtcgagc gtctcgccga cgatcacgcg aacgccgagc gcctcgcgca gggcctcgcg cgcatcgagc cggtgaaggt gctgtcgcag gcgacgaaca tggtgttcgc gcaatttccc gaagccgatt gcgcgccgct cgaagcatgg ctcaaggagc gcgggattct cacgcagatg ctgtatgcgt cgcgcttcgt cacgcactgc gacgtgtcgc gcgacgacat cgacacggct gtcgccgcga tcagcgcgta tttcgcgcgt tcgcgcgctt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.00190.a.A1.PW32454
PURIFICATION DATe: 7/8/2011
CONCENTRATION: 28.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRPRTAHRI GARAPRRARA ASAASSGATP NCVHSKPPLH RASAPAHRAI LLFDETPTGT QMIDLRSDTV TRPSAPMLAA MTAAEVGDDV WGDDPTVARL QAVVAERAGK EAALFFPSGT QSNLAALMSH CERGDEYIVG QAAHTYKYEG GGAAVLGSIQ PQPIENAADG TLPLEKIAAA IKPIDDHFAR SRLFTLENTI GGKVLPAGYV DEAVALARSR GLAAHLDGAR VCNAAVASGR AIAELCAPFD SVSICFSKGL GAPVGSVLVG SGALIERAHR WRKVLGGGMR QAGVLAAACL YALEHNVERL ADDHANAERL AQGLARIEPV KVLSQATNMV FAQFPEADCA PLEAWLKERG ILTQMLYASR FVTHCDVSRD DIDTAVAAIS AYFARSRA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcc cgcgaacggc tcaccgtatc ggcgcgcgag cgccgcgtcg cgcccgcgcc gcttcggccg cgtcaagcgg cgcgacgccg aattgcgtac actcgaagcc tccgcttcac cgcgcgagcg cgccggcgca ccgcgcaatc ctcctcttcg acgagactcc gacgggaacc cagatgatcg atttacgcag cgataccgtg acgcgcccga gcgcgccgat gctggccgcg atgacggccg ccgaagtggg cgacgacgtg tggggcgacg atcccaccgt cgcgcgcctg caggccgtgg tggccgagcg cgcgggcaag gaggcggcgc tgttctttcc gagcggcacg cagagcaacc tcgcggcgct gatgtcgcac tgcgagcgcg gcgacgagta catcgtcggc caggccgcgc acacgtacaa atacgagggc ggcggcgcgg cggtgctcgg cagcattcag ccgcagccga tcgagaacgc cgcggacggc acgctgccgc tcgagaagat cgcggccgcg atcaagccga tcgacgatca cttcgcccgc tcgcgcctgt tcacgcttga gaacacgatc ggcggcaagg tgctgccggc gggctatgtc gacgaagcgg tcgcgctcgc gcgcagccgc gggctcgctg cgcatctcga cggcgcgcgc gtatgcaacg cggccgtcgc gtcgggccgc gcgatcgccg agctgtgcgc gccgttcgat tcggtgtcga tctgcttttc gaaggggctc ggcgcgccgg tgggctcggt gctcgtcggc agcggcgcgc tgatcgagcg cgcgcaccgc tggcgcaagg tgctcggcgg cggcatgcgc caggcgggcg tgctcgcggc cgcgtgcctg tatgcgctcg agcacaacgt cgagcgtctc gccgacgatc acgcgaacgc cgagcgcctc gcgcagggcc tcgcgcgcat cgagccggtg aaggtgctgt cgcaggcgac gaacatggtg ttcgcgcaat ttcccgaagc cgattgcgcg ccgctcgaag catggctcaa ggagcgcggg attctcacgc agatgctgta tgcgtcgcgc ttcgtcacgc actgcgacgt gtcgcgcgac gacatcgaca cggctgtcgc cgcgatcagc gcgtatttcg cgcgttcgcg cgctt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPRTAHRI GARAPRRARA ASAASSGATP NCVHSKPPLH RASAPAHRAI LLFDETPTGT QMIDLRSDTV TRPSAPMLAA MTAAEVGDDV WGDDPTVARL QAVVAERAGK EAALFFPSGT QSNLAALMSH CERGDEYIVG QAAHTYKYEG GGAAVLGSIQ PQPIENAADG TLPLEKIAAA IKPIDDHFAR SRLFTLENTI GGKVLPAGYV DEAVALARSR GLAAHLDGAR VCNAAVASGR AIAELCAPFD SVSICFSKGL GAPVGSVLVG SGALIERAHR WRKVLGGGMR QAGVLAAACL YALEHNVERL ADDHANAERL AQGLARIEPV KVLSQATNMV FAQFPEADCA PLEAWLKERG ILTQMLYASR FVTHCDVSRD DIDTAVAAIS AYFARSRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCCCGCGA ACGGCTCACC GTATCGGCGC GCGAGCGCCG CGTCGCGCCC GCGCCGCTTC GGCCGCGTCA AGCGGCGCGA CGCCGAATTG CGTACACTCG AAGCCTCCGC TTCACCGCGC GAGCGCGCCG GCGCACCGCG CAATCCTCCT CTTCGACGAG ACTCCGACGG GAACCCAGAT GATCGATTTA CGCAGCGATA CCGTGACGCG CCCGAGCGCG CCGATGCTGG CCGCGATGAC GGCCGCCGAA GTGGGCGACG ACGTGTGGGG CGACGATCCC ACCGTCGCGC GCCTGCAGGC CGTGGTGGCC GAGCGCGCGG GCAAGGAGGC GGCGCTGTTC TTTCCGAGCG GCACGCAGAG CAACCTCGCG GCGCTGATGT CGCACTGCGA GCGCGGCGAC GAGTACATCG TCGGCCAGGC CGCGCACACG TACAAATACG AGGGCGGCGG CGCGGCGGTG CTCGGCAGCA TTCAGCCGCA GCCGATCGAG AACGCCGCGG ACGGCACGCT GCCGCTCGAG AAGATCGCGG CCGCGATCAA GCCGATCGAC GATCACTTCG CCCGCTCGCG CCTGTTCACG CTTGAGAACA CGATCGGCGG CAAGGTGCTG CCGGCGGGCT ATGTCGACGA AGCGGTCGCG CTCGCGCGCA GCCGCGGGCT CGCTGCGCAT CTCGACGGCG CGCGCGTATG CAACGCGGCC GTCGCGTCGG GCCGCGCGAT CGCCGAGCTG TGCGCGCCGT TCGATTCGGT GTCGATCTGC TTTTCGAAGG GGCTCGGCGC GCCGGTGGGC TCGGTGCTCG TCGGCAGCGG CGCGCTGATC GAGCGCGCGC ACCGCTGGCG CAAGGTGCTC GGCGGCGGCA TGCGCCAGGC GGGCGTGCTC GCGGCCGCGT GCCTGTATGC GCTCGAGCAC AACGTCGAGC GTCTCGCCGA CGATCACGCG AACGCCGAGC GCCTCGCGCA GGGCCTCGCG CGCATCGAGC CGGTGAAGGT GCTGTCGCAG GCGACGAACA TGGTGTTCGC GCAATTTCCC GAAGCCGATT GCGCGCCGCT CGAAGCATGG CTCAAGGAGC GCGGGATTCT CACGCAGATG CTGTATGCGT CGCGCTTCGT CACGCACTGC GACGTGTCGC GCGACGACAT CGACACGGCT GTCGCCGCGA TCAGCGCGTA TTTCGCGCGT TCGCGCGCTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA