BupsA.00171.b

3-oxoacyl- synthase

CENTER ID: BupsA.00171.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00171.b.A1.GE25597 Full length( BupsA.00171.b ) 1 354
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00171.b.A1.PS00095 Full length( BupsA.00171.b ) 1 354
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_187291
PATRIC ID: fig|320372.6.peg.94
RefSeq: YP_331516.1
UniProt: Q3JY37
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTPHAVAGR EIRLGGARLA GIVSCLPSRE IDNDAFVARF GEGPVRDVVK MIGVERRRWA AEHETTSDLC RAAGAHLLRE LDWRPDSVDA VIFVSQTPDY RLPGTSFVLQ AAWGLPQASI ALDVNLGCSG YPQALWLGTN LIATGAARRV LLAVGDTISK LVDPNDRSTS LLFGDAGTVT ALEADGAAGD AHFVIGADGR GARNLIVPSG ACRPYDAQAD ARLAQRSPGH LFMDGGEIFN FTLQGIPPLV AHTLAFANTS VDQYDAFLFH QANLFMLKHL AKKAGLPAER VPINIGTYGN TSCASIPLLM TTALQAQLAA RPMRLAMFGF GVGYSWASAS LAVEPLSVID NIEL
NT Sequence
GTGAGCACGC CGCACGCCGT CGCGGGACGT GAGATCCGCC TGGGCGGTGC CCGGCTCGCC GGCATCGTCT CGTGCCTGCC GTCGCGCGAG ATCGACAACG ATGCGTTCGT CGCACGCTTC GGCGAGGGAC CGGTCAGGGA CGTCGTCAAG ATGATCGGCG TGGAGCGCCG CCGCTGGGCG GCCGAACACG AGACGACGAG CGATCTGTGC CGCGCGGCCG GCGCGCACCT GCTGCGCGAG CTCGACTGGC GGCCGGACAG CGTCGACGCC GTGATCTTCG TCTCGCAGAC GCCCGACTAC CGGCTACCCG GCACGTCGTT CGTGCTGCAG GCGGCATGGG GCTTGCCGCA GGCGAGCATC GCGCTCGACG TCAACCTCGG CTGCTCCGGC TATCCGCAGG CGCTGTGGCT CGGCACGAAC CTGATCGCCA CGGGCGCCGC GCGGCGCGTG CTGCTCGCCG TCGGCGACAC GATCAGCAAG CTCGTCGATC CGAACGACCG GTCGACGAGC CTGCTGTTCG GCGACGCCGG CACCGTGACG GCGCTCGAAG CCGATGGCGC GGCGGGCGAC GCGCACTTCG TGATCGGCGC GGACGGCCGC GGCGCGCGCA ACCTGATCGT GCCGTCGGGC GCGTGCCGCC CATACGACGC GCAGGCCGAC GCGCGCCTCG CGCAGCGCTC GCCCGGCCAC CTGTTCATGG ACGGCGGCGA GATATTCAAT TTCACGCTGC AAGGCATTCC GCCGCTCGTC GCGCATACGC TCGCGTTCGC GAACACGAGC GTCGACCAGT ACGACGCGTT CCTGTTTCAT CAGGCGAACC TCTTCATGCT GAAGCATCTC GCGAAGAAAG CGGGGCTGCC CGCCGAGCGC GTGCCGATCA ACATCGGCAC ATACGGCAAT ACGAGCTGCG CGTCCATTCC GCTACTGATG ACGACCGCGC TGCAAGCGCA GCTCGCCGCG CGCCCGATGC GGCTCGCGAT GTTCGGCTTC GGCGTCGGCT ATTCGTGGGC GTCGGCATCG CTCGCCGTCG AACCGCTTTC CGTCATCGAC AACATCGAGC TA
Details for BupsA.00171.b.A1.GE25597
HARVESTED ON: 11/19/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 40kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSTPHAVA GREIRLGGAR LAGIVSCLPS REIDNDAFVA RFGEGPVRDV VKMIGVERRR WAAEHETTSD LCRAAGAHLL RELDWRPDSV DAVIFVSQTP DYRLPGTSFV LQAAWGLPQA SIALDVNLGC SGYPQALWLG TNLIATGAAR RVLLAVGDTI SKLVDPNDRS TSLLFGDAGT VTALEADGAA GDAHFVIGAD GRGARNLIVP SGACRPYDAQ ADARLAQRSP GHLFMDGGEI FNFTLQGIPP LVAHTLAFAN TSVDQYDAFL FHQANLFMLK HLAKKAGLPA ERVPINIGTY GNTSCASIPL LMTTALQAQL AARPMRLAMF GFGVGYSWAS ASLAVEPLSV IDNI
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgag cacgccgcac gccgtcgcgg gacgtgagat ccgcctgggc ggtgcccggc tcgccggcat cgtctcgtgc ctgccgtcgc gcgagatcga caacgatgcg ttcgtcgcac gcttcggcga gggaccggtc agggacgtcg tcaagatgat cggcgtggag cgccgccgct gggcggccga acacgagacg acgagcgatc tgtgccgcgc ggccggcgcg cacctgctgc gcgagctcga ctggcggccg gacagcgtcg acgccgtgat cttcgtctcg cagacgcccg actaccggct acccggcacg tcgttcgtgc tgcaggcggc atggggcttg ccgcaggcga gcatcgcgct cgacgtcaac ctcggctgct ccggctatcc gcaggcgctg tggctcggca cgaacctgat cgccacgggc gccgcgcggc gcgtgctgct cgccgtcggc gacacgatca gcaagctcgt cgatccgaac gaccggtcga cgagcctgct gttcggcgac gccggcaccg tgacggcgct cgaagccgat ggcgcggcgg gcgacgcgca cttcgtgatc ggcgcggacg gccgcggcgc gcgcaacctg atcgtgccgt cgggcgcgtg ccgcccatac gacgcgcagg ccgacgcgcg cctcgcgcag cgctcgcccg gccacctgtt catggacggc ggcgagatat tcaatttcac gctgcaaggc attccgccgc tcgtcgcgca tacgctcgcg ttcgcgaaca cgagcgtcga ccagtacgac gcgttcctgt ttcatcaggc gaacctcttc atgctgaagc atctcgcgaa gaaagcgggg ctgcccgccg agcgcgtgcc gatcaacatc ggcacatacg gcaatacgag ctgcgcgtcc attccgctac tgatgacgac cgcgctgcaa gcgcagctcg ccgcgcgccc gatgcggctc gcgatgttcg gcttcggcgt cggctattcg tgggcgtcgg catcgctcgc cgtcgaaccg ctttccgtca tcgacaacat cg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSTPHAVA GREIRLGGAR LAGIVSCLPS REIDNDAFVA RFGEGPVRDV VKMIGVERRR WAAEHETTSD LCRAAGAHLL RELDWRPDSV DAVIFVSQTP DYRLPGTSFV LQAAWGLPQA SIALDVNLGC SGYPQALWLG TNLIATGAAR RVLLAVGDTI SKLVDPNDRS TSLLFGDAGT VTALEADGAA GDAHFVIGAD GRGARNLIVP SGACRPYDAQ ADARLAQRSP GHLFMDGGEI FNFTLQGIPP LVAHTLAFAN TSVDQYDAFL FHQANLFMLK HLAKKAGLPA ERVPINIGTY GNTSCASIPL LMTTALQAQL AARPMRLAMF GFGVGYSWAS ASLAVEPLSV IDNIEL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcacg ccgcacgccg tcgcgggacg tgagatccgc ctgggcggtg cccggctcgc cggcatcgtc tcgtgcctgc cgtcgcgcga gatcgacaac gatgcgttcg tcgcacgctt cggcgaggga ccggtcaggg acgtcgtcaa gatgatcggc gtggagcgcc gccgctgggc ggccgaacac gagacgacga gcgatctgtg ccgcgcggcc ggcgcgcacc tgctgcgcga gctcgactgg cggccggaca gcgtcgacgc cgtgatcttc gtctcgcaga cgcccgacta ccggctaccc ggcacgtcgt tcgtgctgca ggcggcatgg ggcttgccgc aggcgagcat cgcgctcgac gtcaacctcg gctgctccgg ctatccgcag gcgctgtggc tcggcacgaa cctgatcgcc acgggcgccg cgcggcgcgt gctgctcgcc gtcggcgaca cgatcagcaa gctcgtcgat ccgaacgacc ggtcgacgag cctgctgttc ggcgacgccg gcaccgtgac ggcgctcgaa gccgatggcg cggcgggcga cgcgcacttc gtgatcggcg cggacggccg cggcgcgcgc aacctgatcg tgccgtcggg cgcgtgccgc ccatacgacg cgcaggccga cgcgcgcctc gcgcagcgct cgcccggcca cctgttcatg gacggcggcg agatattcaa tttcacgctg caaggcattc cgccgctcgt cgcgcatacg ctcgcgttcg cgaacacgag cgtcgaccag tacgacgcgt tcctgtttca tcaggcgaac ctcttcatgc tgaagcatct cgcgaagaaa gcggggctgc ccgccgagcg cgtgccgatc aacatcggca catacggcaa tacgagctgc gcgtccattc cgctactgat gacgaccgcg ctgcaagcgc agctcgccgc gcgcccgatg cggctcgcga tgttcggctt cggcgtcggc tattcgtggg cgtcggcatc gctcgccgtc gaaccgcttt ccgtcatcga caacatcgag ctaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BupsA.00171.b.A1.PS00095
PURIFICATION DATe: 10/29/2008
CONCENTRATION: 6.12mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSTPHAVA GREIRLGGAR LAGIVSCLPS REIDNDAFVA RFGEGPVRDV VKMIGVERRR WAAEHETTSD LCRAAGAHLL RELDWRPDSV DAVIFVSQTP DYRLPGTSFV LQAAWGLPQA SIALDVNLGC SGYPQALWLG TNLIATGAAR RVLLAVGDTI SKLVDPNDRS TSLLFGDAGT VTALEADGAA GDAHFVIGAD GRGARNLIVP SGACRPYDAQ ADARLAQRSP GHLFMDGGEI FNFTLQGIPP LVAHTLAFAN TSVDQYDAFL FHQANLFMLK HLAKKAGLPA ERVPINIGTY GNTSCASIPL LMTTALQAQL AARPMRLAMF GFGVGYSWAS ASLAVEPLSV IDNI
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgag cacgccgcac gccgtcgcgg gacgtgagat ccgcctgggc ggtgcccggc tcgccggcat cgtctcgtgc ctgccgtcgc gcgagatcga caacgatgcg ttcgtcgcac gcttcggcga gggaccggtc agggacgtcg tcaagatgat cggcgtggag cgccgccgct gggcggccga acacgagacg acgagcgatc tgtgccgcgc ggccggcgcg cacctgctgc gcgagctcga ctggcggccg gacagcgtcg acgccgtgat cttcgtctcg cagacgcccg actaccggct acccggcacg tcgttcgtgc tgcaggcggc atggggcttg ccgcaggcga gcatcgcgct cgacgtcaac ctcggctgct ccggctatcc gcaggcgctg tggctcggca cgaacctgat cgccacgggc gccgcgcggc gcgtgctgct cgccgtcggc gacacgatca gcaagctcgt cgatccgaac gaccggtcga cgagcctgct gttcggcgac gccggcaccg tgacggcgct cgaagccgat ggcgcggcgg gcgacgcgca cttcgtgatc ggcgcggacg gccgcggcgc gcgcaacctg atcgtgccgt cgggcgcgtg ccgcccatac gacgcgcagg ccgacgcgcg cctcgcgcag cgctcgcccg gccacctgtt catggacggc ggcgagatat tcaatttcac gctgcaaggc attccgccgc tcgtcgcgca tacgctcgcg ttcgcgaaca cgagcgtcga ccagtacgac gcgttcctgt ttcatcaggc gaacctcttc atgctgaagc atctcgcgaa gaaagcgggg ctgcccgccg agcgcgtgcc gatcaacatc ggcacatacg gcaatacgag ctgcgcgtcc attccgctac tgatgacgac cgcgctgcaa gcgcagctcg ccgcgcgccc gatgcggctc gcgatgttcg gcttcggcgt cggctattcg tgggcgtcgg catcgctcgc cgtcgaaccg ctttccgtca tcgacaacat cg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSTPHAVA GREIRLGGAR LAGIVSCLPS REIDNDAFVA RFGEGPVRDV VKMIGVERRR WAAEHETTSD LCRAAGAHLL RELDWRPDSV DAVIFVSQTP DYRLPGTSFV LQAAWGLPQA SIALDVNLGC SGYPQALWLG TNLIATGAAR RVLLAVGDTI SKLVDPNDRS TSLLFGDAGT VTALEADGAA GDAHFVIGAD GRGARNLIVP SGACRPYDAQ ADARLAQRSP GHLFMDGGEI FNFTLQGIPP LVAHTLAFAN TSVDQYDAFL FHQANLFMLK HLAKKAGLPA ERVPINIGTY GNTSCASIPL LMTTALQAQL AARPMRLAMF GFGVGYSWAS ASLAVEPLSV IDNIEL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCACG CCGCACGCCG TCGCGGGACG TGAGATCCGC CTGGGCGGTG CCCGGCTCGC CGGCATCGTC TCGTGCCTGC CGTCGCGCGA GATCGACAAC GATGCGTTCG TCGCACGCTT CGGCGAGGGA CCGGTCAGGG ACGTCGTCAA GATGATCGGC GTGGAGCGCC GCCGCTGGGC GGCCGAACAC GAGACGACGA GCGATCTGTG CCGCGCGGCC GGCGCGCACC TGCTGCGCGA GCTCGACTGG CGGCCGGACA GCGTCGACGC CGTGATCTTC GTCTCGCAGA CGCCCGACTA CCGGCTACCC GGCACGTCGT TCGTGCTGCA GGCGGCATGG GGCTTGCCGC AGGCGAGCAT CGCGCTCGAC GTCAACCTCG GCTGCTCCGG CTATCCGCAG GCGCTGTGGC TCGGCACGAA CCTGATCGCC ACGGGCGCCG CGCGGCGCGT GCTGCTCGCC GTCGGCGACA CGATCAGCAA GCTCGTCGAT CCGAACGACC GGTCGACGAG CCTGCTGTTC GGCGACGCCG GCACCGTGAC GGCGCTCGAA GCCGATGGCG CGGCGGGCGA CGCGCACTTC GTGATCGGCG CGGACGGCCG CGGCGCGCGC AACCTGATCG TGCCGTCGGG CGCGTGCCGC CCATACGACG CGCAGGCCGA CGCGCGCCTC GCGCAGCGCT CGCCCGGCCA CCTGTTCATG GACGGCGGCG AGATATTCAA TTTCACGCTG CAAGGCATTC CGCCGCTCGT CGCGCATACG CTCGCGTTCG CGAACACGAG CGTCGACCAG TACGACGCGT TCCTGTTTCA TCAGGCGAAC CTCTTCATGC TGAAGCATCT CGCGAAGAAA GCGGGGCTGC CCGCCGAGCG CGTGCCGATC AACATCGGCA CATACGGCAA TACGAGCTGC GCGTCCATTC CGCTACTGAT GACGACCGCG CTGCAAGCGC AGCTCGCCGC GCGCCCGATG CGGCTCGCGA TGTTCGGCTT CGGCGTCGGC TATTCGTGGG CGTCGGCATC GCTCGCCGTC GAACCGCTTT CCGTCATCGA CAACATCGAG CTAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA