BupsA.00171.a
3-oxoacyl-(acyl-carrier-protein) synthase III
| CENTER ID: | BupsA.00171.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00171.a.A1.GE25596 | Full length( BupsA.00171.a ) | 1 | 344 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00171.a.A1.PS00094 | Full length( BupsA.00171.a ) | 1 | 344 | ||
| BupsA.00171.a.A1.PS00198a | Full length( BupsA.00171.a ) | 1 | 344 | ||
| BupsA.00171.a.A1.PS00201 | Full length( BupsA.00171.a ) | 1 | 344 |
Structures
3GWA
DEPOSITED: 3/31/2009
DETERMINATION: XRay
CLONE: BupsA.00171.a.A1.GE25596
PROTEIN: BupsA.00171.a.A1.PS00094
3GWE
DEPOSITED: 3/31/2009
DETERMINATION: XRay
CLONE: BupsA.00171.a.A1.GE25596
PROTEIN: BupsA.00171.a.A1.PS00094
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_179210 |
| PATRIC ID: | fig|320372.6.peg.92 |
| RefSeq: | YP_331513.1 |
| UniProt: | Q3JY40 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPAAHPPRA AIADIAGHLP EQVLTNDVLA QLYPDWPAEK ILAKTGIRER RIAAPRETAA DLAYEAARKL FAQGAVGADQ VDFVILCTQA PDYVLPTSAC MLQHRLGIPT HAGALDVNLG CSGYVYGLSL AKGLVETGAA RCVLLLTADT YSKYLHPLDK SVRTLFGDGA SATAVIAEHG ELERIGPFVF GTDGRGAPNL IVKAGLFREP KSADSAREHE DASGNVRTDE HLYMNGAEVM AFSLAEVPRA ADRLLALAGE PRENIDCFVL HQANRFMLDA LRKKMKIPEH KFPVLMEHCG NTVSSTLPLA LETMRANGTL ARGMRLMLLG FGVGYSWAGC LVNF
NT Sequence
ATGACACCGG CCGCCCATCC GCCGCGCGCG GCGATCGCGG ACATCGCCGG GCATCTGCCC GAGCAGGTGC TGACGAACGA CGTCCTCGCG CAGCTCTATC CGGACTGGCC GGCGGAGAAG ATCCTCGCGA AGACGGGCAT CCGCGAACGC CGCATCGCCG CTCCCCGCGA GACGGCCGCC GACCTCGCGT ATGAAGCGGC GCGCAAGCTG TTCGCGCAAG GCGCCGTCGG CGCAGACCAG GTCGATTTCG TGATTCTCTG CACGCAGGCG CCCGACTACG TGCTGCCCAC GTCCGCCTGC ATGCTCCAGC ATCGCCTCGG CATTCCGACG CACGCGGGGG CGCTCGACGT CAACCTCGGC TGCTCGGGTT ACGTGTACGG GCTGTCGCTC GCGAAGGGGC TCGTCGAGAC GGGCGCCGCG CGCTGCGTGC TGCTGCTCAC GGCCGACACC TATTCGAAGT ATCTGCACCC GCTCGACAAG AGCGTGCGCA CGCTGTTCGG CGACGGCGCG AGCGCGACCG CCGTGATCGC CGAACACGGC GAGCTCGAGC GTATCGGCCC CTTCGTGTTC GGCACGGACG GCCGCGGCGC GCCGAACCTG ATCGTGAAGG CGGGCCTCTT TCGCGAACCG AAGAGCGCCG ACAGCGCGCG CGAGCACGAG GACGCATCGG GCAACGTGCG CACCGACGAG CATCTATACA TGAACGGCGC GGAGGTGATG GCGTTCTCGC TCGCCGAAGT GCCGCGCGCC GCCGACCGGC TGCTCGCGCT CGCGGGCGAG CCGCGCGAGA ACATCGATTG CTTCGTGCTG CATCAGGCGA ACCGCTTCAT GCTCGATGCG CTGCGCAAGA AAATGAAGAT CCCCGAACAC AAATTCCCCG TGCTGATGGA GCACTGCGGC AATACCGTCT CGTCGACGCT GCCGCTCGCG CTCGAGACGA TGCGGGCAAA CGGCACGCTC GCGCGCGGCA TGCGTCTGAT GCTGCTGGGA TTCGGCGTCG GCTACTCCTG GGCCGGCTGC CTCGTGAACT TT
Details for BupsA.00171.a.A1.GE25596
| HARVESTED ON: | 11/19/2008 |
| SEQUENCED ON: | 3/6/2009 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAAHPPR AAIADIAGHL PEQVLTNDVL AQLYPDWPAE KILAKTGIRE RRIAAPRETA ADLAYEAARK LFAQGAVGAD QVDFVILCTQ APDYVLPTSA CMLQHRLGIP THAGALDVNL GCSGYVYGLS LAKGLVETGA ARCVLLLTAD TYSKYLHPLD KSVRTLFGDG ASATAVIAEH GELERIGPFV FGTDGRGAPN LIVKAGLFRE PKSADSAREH EDASGNVRTD EHLYMNGAEV MAFSLAEVPR AADRLLALAG EPRENIDCFV LHQANRFMLD ALRKKMKIPE HKFPVLMEHC GNTVSSTLPL ALETMRANGT LARGMRLMLL GFGVGYSWAG CLV
Validated NT Sequence
tttaagangg agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacacc ggccgcccat ccgccgcgcg cggcgatcgc ggacatcgcc gggcatctgc ccgagcaggt gctgacgaac gacgtcctcg cgcagctcta tccggactgg ccggcggaga agatcctcgc gaagacgggc atccgcgaac gccgcatcgc cgctccccgc gagacggccg ccgacctcgc gtatgaagcg gcgcgcaagc tgttcgcgca aggcgccgtc ggcgcagacc aggtcgattt cgtgattctc tgcacgcagg cgcccgacta cgtgctgccc acgtccgcct gcatgctcca gcatcgcctc ggcattccga cgcacgcggg ggcgctcgac gtcaacctcg gctgctcggg ttacgtgtac gggctgtcgc tcgcgaaggg gctcgtcgag acgggcgccg cgcgctgcgt gctgctgctc acggccgaca cctattcgaa gtatctgcac ccgctcgaca agagcgtgcg cacgctgttc ggcgacggcg cgagcgcgac cgccgtgatc gccgaacacg gcgagctcga gcgtatcggc cccttcgtgt tcggcacgga cggccgcggc gcgccgaacc tgatcgtgaa ggcgggcctc tttcgcgaac cgaagagcgc cgacagcgcg cgcgagcacg aggacgcatc gggcaacgtg cgcaccgacg agcatctata catgaacggc gcggaggtga tggcgttctc gctcgccgaa gtgccgcgcg ccgccgaccg gctgctcgcg ctcgcgggcg agccgcgcga gaacatcgat tgcttcgtgc tgcatcaggc gaaccgcttc atgctcgatg cgctgcgcaa gaaaatgaag atccccgaac acaaattccc cgtgctgatg gagcactgcg gcaataccgt ctcgtcgacg ctgccgctcg cgctcgagac gatgcgggca aacggcacgc tcgcgcgcgg catgcgtctg atgctgctgg gattcggcgt cggctactcc tgggccggct gcctcgtga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAAHPPR AAIADIAGHL PEQVLTNDVL AQLYPDWPAE KILAKTGIRE RRIAAPRETA ADLAYEAARK LFAQGAVGAD QVDFVILCTQ APDYVLPTSA CMLQHRLGIP THAGALDVNL GCSGYVYGLS LAKGLVETGA ARCVLLLTAD TYSKYLHPLD KSVRTLFGDG ASATAVIAEH GELERIGPFV FGTDGRGAPN LIVKAGLFRE PKSADSAREH EDASGNVRTD EHLYMNGAEV MAFSLAEVPR AADRLLALAG EPRENIDCFV LHQANRFMLD ALRKKMKIPE HKFPVLMEHC GNTVSSTLPL ALETMRANGT LARGMRLMLL GFGVGYSWAG CLVNF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaccggcc gcccatccgc cgcgcgcggc gatcgcggac atcgccgggc atctgcccga gcaggtgctg acgaacgacg tcctcgcgca gctctatccg gactggccgg cggagaagat cctcgcgaag acgggcatcc gcgaacgccg catcgccgct ccccgcgaga cggccgccga cctcgcgtat gaagcggcgc gcaagctgtt cgcgcaaggc gccgtcggcg cagaccaggt cgatttcgtg attctctgca cgcaggcgcc cgactacgtg ctgcccacgt ccgcctgcat gctccagcat cgcctcggca ttccgacgca cgcgggggcg ctcgacgtca acctcggctg ctcgggttac gtgtacgggc tgtcgctcgc gaaggggctc gtcgagacgg gcgccgcgcg ctgcgtgctg ctgctcacgg ccgacaccta ttcgaagtat ctgcacccgc tcgacaagag cgtgcgcacg ctgttcggcg acggcgcgag cgcgaccgcc gtgatcgccg aacacggcga gctcgagcgt atcggcccct tcgtgttcgg cacggacggc cgcggcgcgc cgaacctgat cgtgaaggcg ggcctctttc gcgaaccgaa gagcgccgac agcgcgcgcg agcacgagga cgcatcgggc aacgtgcgca ccgacgagca tctatacatg aacggcgcgg aggtgatggc gttctcgctc gccgaagtgc cgcgcgccgc cgaccggctg ctcgcgctcg cgggcgagcc gcgcgagaac atcgattgct tcgtgctgca tcaggcgaac cgcttcatgc tcgatgcgct gcgcaagaaa atgaagatcc ccgaacacaa attccccgtg ctgatggagc actgcggcaa taccgtctcg tcgacgctgc cgctcgcgct cgagacgatg cgggcaaacg gcacgctcgc gcgcggcatg cgtctgatgc tgctgggatt cggcgtcggc tactcctggg ccggctgcct cgtgaacttt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.00171.a.A1.PS00094
| PURIFICATION DATe: | 10/28/2008 |
| CONCENTRATION: | 51.43mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAAHPPR AAIADIAGHL PEQVLTNDVL AQLYPDWPAE KILAKTGIRE RRIAAPRETA ADLAYEAARK LFAQGAVGAD QVDFVILCTQ APDYVLPTSA CMLQHRLGIP THAGALDVNL GCSGYVYGLS LAKGLVETGA ARCVLLLTAD TYSKYLHPLD KSVRTLFGDG ASATAVIAEH GELERIGPFV FGTDGRGAPN LIVKAGLFRE PKSADSAREH EDASGNVRTD EHLYMNGAEV MAFSLAEVPR AADRLLALAG EPRENIDCFV LHQANRFMLD ALRKKMKIPE HKFPVLMEHC GNTVSSTLPL ALETMRANGT LARGMRLMLL GFGVGYSWAG CLV
Validated NT Sequence
tttaagangg agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacacc ggccgcccat ccgccgcgcg cggcgatcgc ggacatcgcc gggcatctgc ccgagcaggt gctgacgaac gacgtcctcg cgcagctcta tccggactgg ccggcggaga agatcctcgc gaagacgggc atccgcgaac gccgcatcgc cgctccccgc gagacggccg ccgacctcgc gtatgaagcg gcgcgcaagc tgttcgcgca aggcgccgtc ggcgcagacc aggtcgattt cgtgattctc tgcacgcagg cgcccgacta cgtgctgccc acgtccgcct gcatgctcca gcatcgcctc ggcattccga cgcacgcggg ggcgctcgac gtcaacctcg gctgctcggg ttacgtgtac gggctgtcgc tcgcgaaggg gctcgtcgag acgggcgccg cgcgctgcgt gctgctgctc acggccgaca cctattcgaa gtatctgcac ccgctcgaca agagcgtgcg cacgctgttc ggcgacggcg cgagcgcgac cgccgtgatc gccgaacacg gcgagctcga gcgtatcggc cccttcgtgt tcggcacgga cggccgcggc gcgccgaacc tgatcgtgaa ggcgggcctc tttcgcgaac cgaagagcgc cgacagcgcg cgcgagcacg aggacgcatc gggcaacgtg cgcaccgacg agcatctata catgaacggc gcggaggtga tggcgttctc gctcgccgaa gtgccgcgcg ccgccgaccg gctgctcgcg ctcgcgggcg agccgcgcga gaacatcgat tgcttcgtgc tgcatcaggc gaaccgcttc atgctcgatg cgctgcgcaa gaaaatgaag atccccgaac acaaattccc cgtgctgatg gagcactgcg gcaataccgt ctcgtcgacg ctgccgctcg cgctcgagac gatgcgggca aacggcacgc tcgcgcgcgg catgcgtctg atgctgctgg gattcggcgt cggctactcc tgggccggct gcctcgtga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAAHPPR AAIADIAGHL PEQVLTNDVL AQLYPDWPAE KILAKTGIRE RRIAAPRETA ADLAYEAARK LFAQGAVGAD QVDFVILCTQ APDYVLPTSA CMLQHRLGIP THAGALDVNL GCSGYVYGLS LAKGLVETGA ARCVLLLTAD TYSKYLHPLD KSVRTLFGDG ASATAVIAEH GELERIGPFV FGTDGRGAPN LIVKAGLFRE PKSADSAREH EDASGNVRTD EHLYMNGAEV MAFSLAEVPR AADRLLALAG EPRENIDCFV LHQANRFMLD ALRKKMKIPE HKFPVLMEHC GNTVSSTLPL ALETMRANGT LARGMRLMLL GFGVGYSWAG CLVNF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACCGGCC GCCCATCCGC CGCGCGCGGC GATCGCGGAC ATCGCCGGGC ATCTGCCCGA GCAGGTGCTG ACGAACGACG TCCTCGCGCA GCTCTATCCG GACTGGCCGG CGGAGAAGAT CCTCGCGAAG ACGGGCATCC GCGAACGCCG CATCGCCGCT CCCCGCGAGA CGGCCGCCGA CCTCGCGTAT GAAGCGGCGC GCAAGCTGTT CGCGCAAGGC GCCGTCGGCG CAGACCAGGT CGATTTCGTG ATTCTCTGCA CGCAGGCGCC CGACTACGTG CTGCCCACGT CCGCCTGCAT GCTCCAGCAT CGCCTCGGCA TTCCGACGCA CGCGGGGGCG CTCGACGTCA ACCTCGGCTG CTCGGGTTAC GTGTACGGGC TGTCGCTCGC GAAGGGGCTC GTCGAGACGG GCGCCGCGCG CTGCGTGCTG CTGCTCACGG CCGACACCTA TTCGAAGTAT CTGCACCCGC TCGACAAGAG CGTGCGCACG CTGTTCGGCG ACGGCGCGAG CGCGACCGCC GTGATCGCCG AACACGGCGA GCTCGAGCGT ATCGGCCCCT TCGTGTTCGG CACGGACGGC CGCGGCGCGC CGAACCTGAT CGTGAAGGCG GGCCTCTTTC GCGAACCGAA GAGCGCCGAC AGCGCGCGCG AGCACGAGGA CGCATCGGGC AACGTGCGCA CCGACGAGCA TCTATACATG AACGGCGCGG AGGTGATGGC GTTCTCGCTC GCCGAAGTGC CGCGCGCCGC CGACCGGCTG CTCGCGCTCG CGGGCGAGCC GCGCGAGAAC ATCGATTGCT TCGTGCTGCA TCAGGCGAAC CGCTTCATGC TCGATGCGCT GCGCAAGAAA ATGAAGATCC CCGAACACAA ATTCCCCGTG CTGATGGAGC ACTGCGGCAA TACCGTCTCG TCGACGCTGC CGCTCGCGCT CGAGACGATG CGGGCAAACG GCACGCTCGC GCGCGGCATG CGTCTGATGC TGCTGGGATT CGGCGTCGGC TACTCCTGGG CCGGCTGCCT CGTGAACTTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BupsA.00171.a.A1.PS00198a
| PURIFICATION DATe: | 3/20/2009 |
| CONCENTRATION: | 26.19mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAAHPPR AAIADIAGHL PEQVLTNDVL AQLYPDWPAE KILAKTGIRE RRIAAPRETA ADLAYEAARK LFAQGAVGAD QVDFVILCTQ APDYVLPTSA CMLQHRLGIP THAGALDVNL GCSGYVYGLS LAKGLVETGA ARCVLLLTAD TYSKYLHPLD KSVRTLFGDG ASATAVIAEH GELERIGPFV FGTDGRGAPN LIVKAGLFRE PKSADSAREH EDASGNVRTD EHLYMNGAEV MAFSLAEVPR AADRLLALAG EPRENIDCFV LHQANRFMLD ALRKKMKIPE HKFPVLMEHC GNTVSSTLPL ALETMRANGT LARGMRLMLL GFGVGYSWAG CLV
Validated NT Sequence
tttaagangg agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacacc ggccgcccat ccgccgcgcg cggcgatcgc ggacatcgcc gggcatctgc ccgagcaggt gctgacgaac gacgtcctcg cgcagctcta tccggactgg ccggcggaga agatcctcgc gaagacgggc atccgcgaac gccgcatcgc cgctccccgc gagacggccg ccgacctcgc gtatgaagcg gcgcgcaagc tgttcgcgca aggcgccgtc ggcgcagacc aggtcgattt cgtgattctc tgcacgcagg cgcccgacta cgtgctgccc acgtccgcct gcatgctcca gcatcgcctc ggcattccga cgcacgcggg ggcgctcgac gtcaacctcg gctgctcggg ttacgtgtac gggctgtcgc tcgcgaaggg gctcgtcgag acgggcgccg cgcgctgcgt gctgctgctc acggccgaca cctattcgaa gtatctgcac ccgctcgaca agagcgtgcg cacgctgttc ggcgacggcg cgagcgcgac cgccgtgatc gccgaacacg gcgagctcga gcgtatcggc cccttcgtgt tcggcacgga cggccgcggc gcgccgaacc tgatcgtgaa ggcgggcctc tttcgcgaac cgaagagcgc cgacagcgcg cgcgagcacg aggacgcatc gggcaacgtg cgcaccgacg agcatctata catgaacggc gcggaggtga tggcgttctc gctcgccgaa gtgccgcgcg ccgccgaccg gctgctcgcg ctcgcgggcg agccgcgcga gaacatcgat tgcttcgtgc tgcatcaggc gaaccgcttc atgctcgatg cgctgcgcaa gaaaatgaag atccccgaac acaaattccc cgtgctgatg gagcactgcg gcaataccgt ctcgtcgacg ctgccgctcg cgctcgagac gatgcgggca aacggcacgc tcgcgcgcgg catgcgtctg atgctgctgg gattcggcgt cggctactcc tgggccggct gcctcgtga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAAHPPR AAIADIAGHL PEQVLTNDVL AQLYPDWPAE KILAKTGIRE RRIAAPRETA ADLAYEAARK LFAQGAVGAD QVDFVILCTQ APDYVLPTSA CMLQHRLGIP THAGALDVNL GCSGYVYGLS LAKGLVETGA ARCVLLLTAD TYSKYLHPLD KSVRTLFGDG ASATAVIAEH GELERIGPFV FGTDGRGAPN LIVKAGLFRE PKSADSAREH EDASGNVRTD EHLYMNGAEV MAFSLAEVPR AADRLLALAG EPRENIDCFV LHQANRFMLD ALRKKMKIPE HKFPVLMEHC GNTVSSTLPL ALETMRANGT LARGMRLMLL GFGVGYSWAG CLVNF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACCGGCC GCCCATCCGC CGCGCGCGGC GATCGCGGAC ATCGCCGGGC ATCTGCCCGA GCAGGTGCTG ACGAACGACG TCCTCGCGCA GCTCTATCCG GACTGGCCGG CGGAGAAGAT CCTCGCGAAG ACGGGCATCC GCGAACGCCG CATCGCCGCT CCCCGCGAGA CGGCCGCCGA CCTCGCGTAT GAAGCGGCGC GCAAGCTGTT CGCGCAAGGC GCCGTCGGCG CAGACCAGGT CGATTTCGTG ATTCTCTGCA CGCAGGCGCC CGACTACGTG CTGCCCACGT CCGCCTGCAT GCTCCAGCAT CGCCTCGGCA TTCCGACGCA CGCGGGGGCG CTCGACGTCA ACCTCGGCTG CTCGGGTTAC GTGTACGGGC TGTCGCTCGC GAAGGGGCTC GTCGAGACGG GCGCCGCGCG CTGCGTGCTG CTGCTCACGG CCGACACCTA TTCGAAGTAT CTGCACCCGC TCGACAAGAG CGTGCGCACG CTGTTCGGCG ACGGCGCGAG CGCGACCGCC GTGATCGCCG AACACGGCGA GCTCGAGCGT ATCGGCCCCT TCGTGTTCGG CACGGACGGC CGCGGCGCGC CGAACCTGAT CGTGAAGGCG GGCCTCTTTC GCGAACCGAA GAGCGCCGAC AGCGCGCGCG AGCACGAGGA CGCATCGGGC AACGTGCGCA CCGACGAGCA TCTATACATG AACGGCGCGG AGGTGATGGC GTTCTCGCTC GCCGAAGTGC CGCGCGCCGC CGACCGGCTG CTCGCGCTCG CGGGCGAGCC GCGCGAGAAC ATCGATTGCT TCGTGCTGCA TCAGGCGAAC CGCTTCATGC TCGATGCGCT GCGCAAGAAA ATGAAGATCC CCGAACACAA ATTCCCCGTG CTGATGGAGC ACTGCGGCAA TACCGTCTCG TCGACGCTGC CGCTCGCGCT CGAGACGATG CGGGCAAACG GCACGCTCGC GCGCGGCATG CGTCTGATGC TGCTGGGATT CGGCGTCGGC TACTCCTGGG CCGGCTGCCT CGTGAACTTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BupsA.00171.a.A1.PS00201
| PURIFICATION DATe: | 4/10/2009 |
| CONCENTRATION: | 35.41mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAAHPPR AAIADIAGHL PEQVLTNDVL AQLYPDWPAE KILAKTGIRE RRIAAPRETA ADLAYEAARK LFAQGAVGAD QVDFVILCTQ APDYVLPTSA CMLQHRLGIP THAGALDVNL GCSGYVYGLS LAKGLVETGA ARCVLLLTAD TYSKYLHPLD KSVRTLFGDG ASATAVIAEH GELERIGPFV FGTDGRGAPN LIVKAGLFRE PKSADSAREH EDASGNVRTD EHLYMNGAEV MAFSLAEVPR AADRLLALAG EPRENIDCFV LHQANRFMLD ALRKKMKIPE HKFPVLMEHC GNTVSSTLPL ALETMRANGT LARGMRLMLL GFGVGYSWAG CLV
Validated NT Sequence
tttaagangg agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacacc ggccgcccat ccgccgcgcg cggcgatcgc ggacatcgcc gggcatctgc ccgagcaggt gctgacgaac gacgtcctcg cgcagctcta tccggactgg ccggcggaga agatcctcgc gaagacgggc atccgcgaac gccgcatcgc cgctccccgc gagacggccg ccgacctcgc gtatgaagcg gcgcgcaagc tgttcgcgca aggcgccgtc ggcgcagacc aggtcgattt cgtgattctc tgcacgcagg cgcccgacta cgtgctgccc acgtccgcct gcatgctcca gcatcgcctc ggcattccga cgcacgcggg ggcgctcgac gtcaacctcg gctgctcggg ttacgtgtac gggctgtcgc tcgcgaaggg gctcgtcgag acgggcgccg cgcgctgcgt gctgctgctc acggccgaca cctattcgaa gtatctgcac ccgctcgaca agagcgtgcg cacgctgttc ggcgacggcg cgagcgcgac cgccgtgatc gccgaacacg gcgagctcga gcgtatcggc cccttcgtgt tcggcacgga cggccgcggc gcgccgaacc tgatcgtgaa ggcgggcctc tttcgcgaac cgaagagcgc cgacagcgcg cgcgagcacg aggacgcatc gggcaacgtg cgcaccgacg agcatctata catgaacggc gcggaggtga tggcgttctc gctcgccgaa gtgccgcgcg ccgccgaccg gctgctcgcg ctcgcgggcg agccgcgcga gaacatcgat tgcttcgtgc tgcatcaggc gaaccgcttc atgctcgatg cgctgcgcaa gaaaatgaag atccccgaac acaaattccc cgtgctgatg gagcactgcg gcaataccgt ctcgtcgacg ctgccgctcg cgctcgagac gatgcgggca aacggcacgc tcgcgcgcgg catgcgtctg atgctgctgg gattcggcgt cggctactcc tgggccggct gcctcgtga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAAHPPR AAIADIAGHL PEQVLTNDVL AQLYPDWPAE KILAKTGIRE RRIAAPRETA ADLAYEAARK LFAQGAVGAD QVDFVILCTQ APDYVLPTSA CMLQHRLGIP THAGALDVNL GCSGYVYGLS LAKGLVETGA ARCVLLLTAD TYSKYLHPLD KSVRTLFGDG ASATAVIAEH GELERIGPFV FGTDGRGAPN LIVKAGLFRE PKSADSAREH EDASGNVRTD EHLYMNGAEV MAFSLAEVPR AADRLLALAG EPRENIDCFV LHQANRFMLD ALRKKMKIPE HKFPVLMEHC GNTVSSTLPL ALETMRANGT LARGMRLMLL GFGVGYSWAG CLVNF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACCGGCC GCCCATCCGC CGCGCGCGGC GATCGCGGAC ATCGCCGGGC ATCTGCCCGA GCAGGTGCTG ACGAACGACG TCCTCGCGCA GCTCTATCCG GACTGGCCGG CGGAGAAGAT CCTCGCGAAG ACGGGCATCC GCGAACGCCG CATCGCCGCT CCCCGCGAGA CGGCCGCCGA CCTCGCGTAT GAAGCGGCGC GCAAGCTGTT CGCGCAAGGC GCCGTCGGCG CAGACCAGGT CGATTTCGTG ATTCTCTGCA CGCAGGCGCC CGACTACGTG CTGCCCACGT CCGCCTGCAT GCTCCAGCAT CGCCTCGGCA TTCCGACGCA CGCGGGGGCG CTCGACGTCA ACCTCGGCTG CTCGGGTTAC GTGTACGGGC TGTCGCTCGC GAAGGGGCTC GTCGAGACGG GCGCCGCGCG CTGCGTGCTG CTGCTCACGG CCGACACCTA TTCGAAGTAT CTGCACCCGC TCGACAAGAG CGTGCGCACG CTGTTCGGCG ACGGCGCGAG CGCGACCGCC GTGATCGCCG AACACGGCGA GCTCGAGCGT ATCGGCCCCT TCGTGTTCGG CACGGACGGC CGCGGCGCGC CGAACCTGAT CGTGAAGGCG GGCCTCTTTC GCGAACCGAA GAGCGCCGAC AGCGCGCGCG AGCACGAGGA CGCATCGGGC AACGTGCGCA CCGACGAGCA TCTATACATG AACGGCGCGG AGGTGATGGC GTTCTCGCTC GCCGAAGTGC CGCGCGCCGC CGACCGGCTG CTCGCGCTCG CGGGCGAGCC GCGCGAGAAC ATCGATTGCT TCGTGCTGCA TCAGGCGAAC CGCTTCATGC TCGATGCGCT GCGCAAGAAA ATGAAGATCC CCGAACACAA ATTCCCCGTG CTGATGGAGC ACTGCGGCAA TACCGTCTCG TCGACGCTGC CGCTCGCGCT CGAGACGATG CGGGCAAACG GCACGCTCGC GCGCGGCATG CGTCTGATGC TGCTGGGATT CGGCGTCGGC TACTCCTGGG CCGGCTGCCT CGTGAACTTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA