BupsA.00166.b

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase

CENTER ID: BupsA.00166.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00166.b.A1.GE25594 Full length( BupsA.00166.b ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3682
RefSeq: YP_334907.1
UniProt: Q3JNE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MISSIKKTVT MLKQRTIKSI VKTVGIGVHS GRKVELTLRP AAPDTGIVFS RVDLPTPVDI PASALSIGDT RLASVLQKDG VRVSTVEHLM SACAGLGIDN LYVDVTAEEI PIMDGSAATF VFLIQSAGIE EQNAAKKFIK VTKPVEIRDG DKFARLDPYF GFKLKFTIDF RHPAVDKTGQ ELEVDFANTS YVREIARART FGFAHEVEMM RELGLARGGS MDNAIVLDEY RILNNDGLRY DDEFVKHKML DAIGDLYVIG HPLLASYTAY KSGHGLNNAL LRELLAHEQA YEIVTFDDPK TAPTGFGFDA QTAFA
NT Sequence
TTGATATCCT CAATCAAGAA GACTGTGACC ATGTTGAAGC AGCGCACCAT CAAATCGATC GTCAAGACCG TCGGCATCGG CGTGCATTCG GGCCGCAAGG TCGAACTGAC GCTTCGTCCC GCGGCGCCCG ATACGGGCAT CGTGTTCTCG CGCGTCGATC TGCCGACGCC CGTCGACATT CCCGCTTCGG CGCTGTCGAT CGGCGACACG CGGCTCGCGT CCGTGCTGCA GAAGGACGGC GTGCGCGTGT CGACGGTCGA GCACCTGATG TCGGCGTGCG CGGGCCTCGG CATCGACAAC CTCTACGTCG ACGTGACGGC CGAGGAAATT CCGATCATGG ACGGCAGCGC GGCGACGTTC GTGTTTCTGA TCCAGTCGGC CGGCATCGAA GAGCAGAACG CGGCGAAGAA GTTCATCAAG GTGACGAAGC CCGTCGAGAT CCGCGACGGC GATAAATTCG CGCGTCTCGA TCCGTACTTC GGTTTCAAGC TGAAGTTCAC GATCGATTTC CGCCATCCGG CCGTCGACAA GACGGGCCAG GAGCTCGAGG TCGATTTCGC GAACACGTCG TACGTGCGCG AGATCGCGCG CGCGCGCACG TTCGGCTTCG CGCACGAGGT CGAGATGATG CGCGAGCTGG GGCTCGCGCG CGGCGGCAGC ATGGACAACG CGATCGTGCT CGACGAGTAC CGGATCCTCA ACAACGACGG CCTTCGCTAC GACGACGAGT TCGTCAAGCA CAAGATGCTC GACGCGATCG GTGATCTGTA CGTGATCGGC CACCCGCTGC TCGCGTCTTA CACGGCGTAC AAGTCGGGCC ACGGGCTGAA CAACGCGTTG CTGCGCGAAT TGCTCGCGCA TGAGCAAGCG TATGAGATCG TCACGTTCGA CGATCCGAAG ACGGCGCCGA CGGGGTTCGG CTTCGACGCG CAGACGGCGT TCGCG
Details for BupsA.00166.b.A1.GE25594
HARVESTED ON: 9/10/2008
SEQUENCED ON: 3/6/2009
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLISSIKKT VTMLKQRTIK SIVKTVGIGV HSGRKVELTL RPAAPDTGIV FSRVDLPTPV DIPASALSIG DTRLASVLQK DGVRVSTVEH LMSACAGLGI DNLYVDVTAE EIPIMDGSAA TFVFLIQSAG IEEQNAAKKF IKVTKPVEIR DGDKFARLDP YFGFKLKFTI DFRHPAVDKT GQELEVDFAN TSYVREIARA RTFGFAHEVE MMRELGLARG GSMDNAIVLD EYRILNNDGL RYDDEFVKHK MLDAIGDLYV IGHPLLASYT AYKSGHGLNN ALLRELLAHE QAYEIVTFDD PKTAPTGFGF DAQTAFA
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttgata tcctcaatca agaagactgt gaccatgttg aagcagcgca ccatcaaatc gatcgtcaag accgtcggca tcggcgtgca ttcgggccgc aaggtcgaac tgacgcttcg tcccgcggcg cccgatacgg gcatcgtgtt ctcgcgcgtc gatctgccga cgcccgtcga cattcccgct tcggcgctgt cgatcggcga cacgcggctc gcgtccgtgc tgcagaagga cggcgtgcgc gtgtcgacgg tcgagcacct gatgtcggcg tgcgcgggcc tcggcatcga caacctctac gtcgacgtga cggccgagga aattccgatc atggacggca gcgcggcgac gttcgtgttt ctgatccagt cggccggcat cgaagagcag aacgcggcga agaagttcat caaggtgacg aagcccgtcg agatccgcga cggcgataaa ttcgcgcgtc tcgatccgta cttcggtttc aagctgaagt tcacgatcga tttccgccat ccggccgtcg acaagacggg ccaggagctc gaggtcgatt tcgcgaacac gtcgtacgtg cgcgagatcg cgcgcgcgcg cacgttcggc ttcgcgcacg aggtcgagat gatgcgcgag ctggggctcg cgcgcggcgg cagcatggac aacgcgatcg tgctcgacga gtaccggatc ctcaacaacg acggccttcg ctacgacgac gagttcgtca agcacaagat gctcgacgcg atcggtgatc tgtacgtgat cggccacccg ctgctcgcgt cttacacggc gtacaagtcg ggccacgggc tgaacaacgc gttgctgcgc gaattgctcg cgcatgagca agcgtatgag atcgtcacgt tcgacgatcc gaagacggcg ccgacggggt tcggcttcga cgcgcagacg gcgttcgcgt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLISSIKKT VTMLKQRTIK SIVKTVGIGV HSGRKVELTL RPAAPDTGIV FSRVDLPTPV DIPASALSIG DTRLASVLQK DGVRVSTVEH LMSACAGLGI DNLYVDVTAE EIPIMDGSAA TFVFLIQSAG IEEQNAAKKF IKVTKPVEIR DGDKFARLDP YFGFKLKFTI DFRHPAVDKT GQELEVDFAN TSYVREIARA RTFGFAHEVE MMRELGLARG GSMDNAIVLD EYRILNNDGL RYDDEFVKHK MLDAIGDLYV IGHPLLASYT AYKSGHGLNN ALLRELLAHE QAYEIVTFDD PKTAPTGFGF DAQTAFA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgatatcc tcaatcaaga agactgtgac catgttgaag cagcgcacca tcaaatcgat cgtcaagacc gtcggcatcg gcgtgcattc gggccgcaag gtcgaactga cgcttcgtcc cgcggcgccc gatacgggca tcgtgttctc gcgcgtcgat ctgccgacgc ccgtcgacat tcccgcttcg gcgctgtcga tcggcgacac gcggctcgcg tccgtgctgc agaaggacgg cgtgcgcgtg tcgacggtcg agcacctgat gtcggcgtgc gcgggcctcg gcatcgacaa cctctacgtc gacgtgacgg ccgaggaaat tccgatcatg gacggcagcg cggcgacgtt cgtgtttctg atccagtcgg ccggcatcga agagcagaac gcggcgaaga agttcatcaa ggtgacgaag cccgtcgaga tccgcgacgg cgataaattc gcgcgtctcg atccgtactt cggtttcaag ctgaagttca cgatcgattt ccgccatccg gccgtcgaca agacgggcca ggagctcgag gtcgatttcg cgaacacgtc gtacgtgcgc gagatcgcgc gcgcgcgcac gttcggcttc gcgcacgagg tcgagatgat gcgcgagctg gggctcgcgc gcggcggcag catggacaac gcgatcgtgc tcgacgagta ccggatcctc aacaacgacg gccttcgcta cgacgacgag ttcgtcaagc acaagatgct cgacgcgatc ggtgatctgt acgtgatcgg ccacccgctg ctcgcgtctt acacggcgta caagtcgggc cacgggctga acaacgcgtt gctgcgcgaa ttgctcgcgc atgagcaagc gtatgagatc gtcacgttcg acgatccgaa gacggcgccg acggggttcg gcttcgacgc gcagacggcg ttcgcgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa