BupsA.00160.a

chorismate mutase

CENTER ID: BupsA.00160.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00160.a.E1.PS00213 Full length( BupsA.00160.a ) 1 205
BupsA.00160.a.E1.PS00405 Full length( BupsA.00160.a ) 1 205
BupsA.00160.a.E1.PS00406 Full length( BupsA.00160.a ) 1 205

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3172
RefSeq: YP_334416.1
UniProt: Q3JPT7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPASPSFTMK QSLRASLAAA VLGCIVLSAP RIAAADGDDT ALTNLVALAS QRLALAEPVA HWKWLNGKPI SDPPREAALL ADVEQRATAN GVDPAYARAF FDDQIAASKQ VQNALFATWR ATHGPEGPAP DLATSTRPQL DRLTQSLIAA LARVAPLRDA PDCPSRLARS VSNWKTLTRY DSGRKDALGT ALSHVCTAGS TSAVG
NT Sequence
GTGCCGGCAT CGCCATCGTT CACCATGAAA CAGTCGCTTC GTGCAAGCTT GGCCGCCGCC GTGCTCGGCT GCATCGTGCT GAGCGCGCCC CGCATCGCCG CCGCCGACGG CGACGATACC GCGCTCACGA ACCTCGTCGC GCTCGCGTCG CAGCGCCTCG CGCTCGCCGA ACCCGTCGCC CACTGGAAGT GGCTCAACGG AAAACCGATC TCGGATCCAC CGCGCGAGGC AGCGCTCCTG GCCGACGTCG AGCAACGCGC GACGGCGAAC GGCGTCGATC CCGCATACGC CCGCGCGTTC TTCGACGACC AGATCGCGGC GAGCAAGCAA GTGCAGAACG CGCTCTTCGC GACATGGCGC GCGACGCACG GCCCGGAAGG CCCCGCGCCC GACTTGGCGA CGAGCACGCG GCCGCAGCTC GACCGCCTCA CGCAATCGCT GATCGCCGCG CTCGCGCGTG TCGCCCCGCT GCGCGACGCG CCGGATTGTC CGTCACGCCT CGCGCGCAGC GTCTCGAACT GGAAGACCTT GACGCGCTAC GACTCCGGCC GCAAGGACGC GCTCGGCACC GCGCTGTCGC ACGTCTGCAC GGCGGGCAGC ACCAGCGCGG TCGGC
Details for BupsA.00160.a.E1.PS00213
PURIFICATION DATe: 5/7/2009
CONCENTRATION: 4.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 25µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVPASPS FTMKQSLRAS LAAAVLGCIV LSAPRIAAAD GDDTALTNLV ALASQRLALA EPVAHWKWLN GKPISDPPRE AALLADVEQR ATANGVDPAY ARAFFDDQIA ASKQVQNALF ATWRATHGPE GPAPDLATST RPQLDRLTQS LIAALARVAP LRDAPDCPSR LARSVSNWKX LTRYDSGRKD ALGTALSHVC TAGSTSAVG
Validated NT Sequence
caccaccacc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgccg gcatcgccat cgttcaccat gaaacagtcg cttcgtgcaa gcttggccgc cgccgtgctc ggctgcatcg tgctgagcgc gccccgcatc gccgccgccg acggcgacga taccgcgctc acgaacctcg tcgcgctcgc gtcgcagcgc ctcgcgctcg ccgaacccgt cgcccactgg aagtggctca acggaaaacc gatctcggat ccaccgcgcg aggcagcgct cctggccgac gtcgagcaac gcgcgacggc gaacggcgtc gatcccgcat acgcccgcgc gttcttcgac gaccagatcg cggcgagcaa gcaagtgcag aacgcgctct tcgcgacatg gcgcgcgacg cacggcccgg aaggccccgc gcccgacttg gcgacgagca cgcggccgca gctcgaccgc ctcacgcaat cgctgatcgc cgcgctcgcg cgtgtcgccc cgctgcgcga cgcgccggat tgtccgtcac gcctcgcgcg cagcgtctcg aactggaaga ncttgacgcg ctacgactcc ggccgcaagg acgcgctcgg caccgcgctg tcgcacgtct gcacggcggg cagcaccagc gcggtcggct aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SVPASPSFTM KQSLRASLAA AVLGCIVLSA PRIAAADGDD TALTNLVALA SQRLALAEPV AHWKWLNGKP ISDPPREAAL LADVEQRATA NGVDPAYARA FFDDQIAASK QVQNALFATW RATHGPEGPA PDLATSTRPQ LDRLTQSLIA ALARVAPLRD APDCPSRLAR SVSNWKTLTR YDSGRKDALG TALSHVCTAG STSAVG
Full NT Sequence (Expression Vector + Insert)
GAACTCACCT ATCTCCCCAA CACCTAATAA CATTCAATCA CTCTTTCCAC TAACCACCTA TCTACATCAC CAAGATATCA CTAGTTCTCG AGATGGCTCA CCACCACCAC CACCATATGG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGTGCC GGCATCGCCA TCGTTCACCA TGAAACAGTC GCTTCGTGCA AGCTTGGCCG CCGCCGTGCT CGGCTGCATC GTGCTGAGCG CGCCCCGCAT CGCCGCCGCC GACGGCGACG ATACCGCGCT CACGAACCTC GTCGCGCTCG CGTCGCAGCG CCTCGCGCTC GCCGAACCCG TCGCCCACTG GAAGTGGCTC AACGGAAAAC CGATCTCGGA TCCACCGCGC GAGGCAGCGC TCCTGGCCGA CGTCGAGCAA CGCGCGACGG CGAACGGCGT CGATCCCGCA TACGCCCGCG CGTTCTTCGA CGACCAGATC GCGGCGAGCA AGCAAGTGCA GAACGCGCTC TTCGCGACAT GGCGCGCGAC GCACGGCCCG GAAGGCCCCG CGCCCGACTT GGCGACGAGC ACGCGGCCGC AGCTCGACCG CCTCACGCAA TCGCTGATCG CCGCGCTCGC GCGTGTCGCC CCGCTGCGCG ACGCGCCGGA TTGTCCGTCA CGCCTCGCGC GCAGCGTCTC GAACTGGAAG ACCTTGACGC GCTACGACTC CGGCCGCAAG GACGCGCTCG GCACCGCGCT GTCGCACGTC TGCACGGCGG GCAGCACCAG CGCGGTCGGC ACGCGTTAAC CACGTGAGTA AGATAGGGAT CCATATATAG GGCCCGGGTT ATAATTACCT CAGGTCGACG TCCCATGGTT TTGTATAGAA TTTACGGCTA GCGCCGGATG CGACGCCGGT CGCGTCTTAT CCGGCCTTCC TATATCAGGC GGTGTTTAAG ACGCCGCCGC TTCGCCCAAA TCCTTATGCC GGTTCGACGA CTGGACAAAA TACTGTTTAT CTTCCCAGCG CAGGCAGGTT AATGTACCAC CCCAGCAGCA GCCGGTATCC AGCGCGTATA TACCTTCCGG CGTACCTTTG CCCTCCAGCG ATGCCCAGTG ACCAAAGGCG ATGCTGTATT CTTCAGCGAC AGGGCCAGGA ATCGCAAACC ACGGTTTCAG TGGGGCAGGG GCCTCTTCCG GCGATTCTTA CTAGCTAGTA TGCATAGGTG CTGAAATATA AAGTTTGTGT TTCTAAAACA CACTTGGTAC GTACGATAAC GTACAGTGTT TTTCCCTCCA CTTAAATCGA AGGGTAGTGT CTTGGAGCGC GCGGAGTAAA CATATATGGT TCATATATGT CCGTAGGCAC GTAAAAAAAG CGAGGGATTC GAATTCCCCC GGAACCCCCG GTTGGGGCCC ACGCCTCGAT CGAGCAAAAA AAAAAAAAAA GAAAAAAAAA AAAAAAAAAA GCTTTCCCGC GGCCAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCACTCA CTGACTCGCT GCGCTCGGTC GCTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGCGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCTG GCTAGCGATG ACCCTGCTGA TTGGTTCGCT GACCATTTCC GGGTGCGGGA CGGCGTTACC AGAAACTCAG AAGGTTCGTC CAACCAAACC GACTCTGGCG GCAGTTTACG AGAGAGATGA TAGGGTCTGC TTCAGTAAGC CAGATGCTAC ACAATTAGGC TTGTACATAC TGTCGTTAGA ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC ATACGATTTA GGTGACACTA TA
Details for BupsA.00160.a.E1.PS00405
PURIFICATION DATe: 11/9/2009
CONCENTRATION: 1.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVPASPS FTMKQSLRAS LAAAVLGCIV LSAPRIAAAD GDDTALTNLV ALASQRLALA EPVAHWKWLN GKPISDPPRE AALLADVEQR ATANGVDPAY ARAFFDDQIA ASKQVQNALF ATWRATHGPE GPAPDLATST RPQLDRLTQS LIAALARVAP LRDAPDCPSR LARSVSNWKX LTRYDSGRKD ALGTALSHVC TAGSTSAVG
Validated NT Sequence
caccaccacc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgccg gcatcgccat cgttcaccat gaaacagtcg cttcgtgcaa gcttggccgc cgccgtgctc ggctgcatcg tgctgagcgc gccccgcatc gccgccgccg acggcgacga taccgcgctc acgaacctcg tcgcgctcgc gtcgcagcgc ctcgcgctcg ccgaacccgt cgcccactgg aagtggctca acggaaaacc gatctcggat ccaccgcgcg aggcagcgct cctggccgac gtcgagcaac gcgcgacggc gaacggcgtc gatcccgcat acgcccgcgc gttcttcgac gaccagatcg cggcgagcaa gcaagtgcag aacgcgctct tcgcgacatg gcgcgcgacg cacggcccgg aaggccccgc gcccgacttg gcgacgagca cgcggccgca gctcgaccgc ctcacgcaat cgctgatcgc cgcgctcgcg cgtgtcgccc cgctgcgcga cgcgccggat tgtccgtcac gcctcgcgcg cagcgtctcg aactggaaga ncttgacgcg ctacgactcc ggccgcaagg acgcgctcgg caccgcgctg tcgcacgtct gcacggcggg cagcaccagc gcggtcggct aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SVPASPSFTM KQSLRASLAA AVLGCIVLSA PRIAAADGDD TALTNLVALA SQRLALAEPV AHWKWLNGKP ISDPPREAAL LADVEQRATA NGVDPAYARA FFDDQIAASK QVQNALFATW RATHGPEGPA PDLATSTRPQ LDRLTQSLIA ALARVAPLRD APDCPSRLAR SVSNWKTLTR YDSGRKDALG TALSHVCTAG STSAVG
Full NT Sequence (Expression Vector + Insert)
GAACTCACCT ATCTCCCCAA CACCTAATAA CATTCAATCA CTCTTTCCAC TAACCACCTA TCTACATCAC CAAGATATCA CTAGTTCTCG AGATGGCTCA CCACCACCAC CACCATATGG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGTGCC GGCATCGCCA TCGTTCACCA TGAAACAGTC GCTTCGTGCA AGCTTGGCCG CCGCCGTGCT CGGCTGCATC GTGCTGAGCG CGCCCCGCAT CGCCGCCGCC GACGGCGACG ATACCGCGCT CACGAACCTC GTCGCGCTCG CGTCGCAGCG CCTCGCGCTC GCCGAACCCG TCGCCCACTG GAAGTGGCTC AACGGAAAAC CGATCTCGGA TCCACCGCGC GAGGCAGCGC TCCTGGCCGA CGTCGAGCAA CGCGCGACGG CGAACGGCGT CGATCCCGCA TACGCCCGCG CGTTCTTCGA CGACCAGATC GCGGCGAGCA AGCAAGTGCA GAACGCGCTC TTCGCGACAT GGCGCGCGAC GCACGGCCCG GAAGGCCCCG CGCCCGACTT GGCGACGAGC ACGCGGCCGC AGCTCGACCG CCTCACGCAA TCGCTGATCG CCGCGCTCGC GCGTGTCGCC CCGCTGCGCG ACGCGCCGGA TTGTCCGTCA CGCCTCGCGC GCAGCGTCTC GAACTGGAAG ACCTTGACGC GCTACGACTC CGGCCGCAAG GACGCGCTCG GCACCGCGCT GTCGCACGTC TGCACGGCGG GCAGCACCAG CGCGGTCGGC ACGCGTTAAC CACGTGAGTA AGATAGGGAT CCATATATAG GGCCCGGGTT ATAATTACCT CAGGTCGACG TCCCATGGTT TTGTATAGAA TTTACGGCTA GCGCCGGATG CGACGCCGGT CGCGTCTTAT CCGGCCTTCC TATATCAGGC GGTGTTTAAG ACGCCGCCGC TTCGCCCAAA TCCTTATGCC GGTTCGACGA CTGGACAAAA TACTGTTTAT CTTCCCAGCG CAGGCAGGTT AATGTACCAC CCCAGCAGCA GCCGGTATCC AGCGCGTATA TACCTTCCGG CGTACCTTTG CCCTCCAGCG ATGCCCAGTG ACCAAAGGCG ATGCTGTATT CTTCAGCGAC AGGGCCAGGA ATCGCAAACC ACGGTTTCAG TGGGGCAGGG GCCTCTTCCG GCGATTCTTA CTAGCTAGTA TGCATAGGTG CTGAAATATA AAGTTTGTGT TTCTAAAACA CACTTGGTAC GTACGATAAC GTACAGTGTT TTTCCCTCCA CTTAAATCGA AGGGTAGTGT CTTGGAGCGC GCGGAGTAAA CATATATGGT TCATATATGT CCGTAGGCAC GTAAAAAAAG CGAGGGATTC GAATTCCCCC GGAACCCCCG GTTGGGGCCC ACGCCTCGAT CGAGCAAAAA AAAAAAAAAA GAAAAAAAAA AAAAAAAAAA GCTTTCCCGC GGCCAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCACTCA CTGACTCGCT GCGCTCGGTC GCTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGCGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCTG GCTAGCGATG ACCCTGCTGA TTGGTTCGCT GACCATTTCC GGGTGCGGGA CGGCGTTACC AGAAACTCAG AAGGTTCGTC CAACCAAACC GACTCTGGCG GCAGTTTACG AGAGAGATGA TAGGGTCTGC TTCAGTAAGC CAGATGCTAC ACAATTAGGC TTGTACATAC TGTCGTTAGA ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC ATACGATTTA GGTGACACTA TA
Details for BupsA.00160.a.E1.PS00406
PURIFICATION DATe: 11/14/2009
CONCENTRATION: 4.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 50µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVPASPS FTMKQSLRAS LAAAVLGCIV LSAPRIAAAD GDDTALTNLV ALASQRLALA EPVAHWKWLN GKPISDPPRE AALLADVEQR ATANGVDPAY ARAFFDDQIA ASKQVQNALF ATWRATHGPE GPAPDLATST RPQLDRLTQS LIAALARVAP LRDAPDCPSR LARSVSNWKX LTRYDSGRKD ALGTALSHVC TAGSTSAVG
Validated NT Sequence
caccaccacc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgccg gcatcgccat cgttcaccat gaaacagtcg cttcgtgcaa gcttggccgc cgccgtgctc ggctgcatcg tgctgagcgc gccccgcatc gccgccgccg acggcgacga taccgcgctc acgaacctcg tcgcgctcgc gtcgcagcgc ctcgcgctcg ccgaacccgt cgcccactgg aagtggctca acggaaaacc gatctcggat ccaccgcgcg aggcagcgct cctggccgac gtcgagcaac gcgcgacggc gaacggcgtc gatcccgcat acgcccgcgc gttcttcgac gaccagatcg cggcgagcaa gcaagtgcag aacgcgctct tcgcgacatg gcgcgcgacg cacggcccgg aaggccccgc gcccgacttg gcgacgagca cgcggccgca gctcgaccgc ctcacgcaat cgctgatcgc cgcgctcgcg cgtgtcgccc cgctgcgcga cgcgccggat tgtccgtcac gcctcgcgcg cagcgtctcg aactggaaga ncttgacgcg ctacgactcc ggccgcaagg acgcgctcgg caccgcgctg tcgcacgtct gcacggcggg cagcaccagc gcggtcggct aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SVPASPSFTM KQSLRASLAA AVLGCIVLSA PRIAAADGDD TALTNLVALA SQRLALAEPV AHWKWLNGKP ISDPPREAAL LADVEQRATA NGVDPAYARA FFDDQIAASK QVQNALFATW RATHGPEGPA PDLATSTRPQ LDRLTQSLIA ALARVAPLRD APDCPSRLAR SVSNWKTLTR YDSGRKDALG TALSHVCTAG STSAVG
Full NT Sequence (Expression Vector + Insert)
GAACTCACCT ATCTCCCCAA CACCTAATAA CATTCAATCA CTCTTTCCAC TAACCACCTA TCTACATCAC CAAGATATCA CTAGTTCTCG AGATGGCTCA CCACCACCAC CACCATATGG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGTGCC GGCATCGCCA TCGTTCACCA TGAAACAGTC GCTTCGTGCA AGCTTGGCCG CCGCCGTGCT CGGCTGCATC GTGCTGAGCG CGCCCCGCAT CGCCGCCGCC GACGGCGACG ATACCGCGCT CACGAACCTC GTCGCGCTCG CGTCGCAGCG CCTCGCGCTC GCCGAACCCG TCGCCCACTG GAAGTGGCTC AACGGAAAAC CGATCTCGGA TCCACCGCGC GAGGCAGCGC TCCTGGCCGA CGTCGAGCAA CGCGCGACGG CGAACGGCGT CGATCCCGCA TACGCCCGCG CGTTCTTCGA CGACCAGATC GCGGCGAGCA AGCAAGTGCA GAACGCGCTC TTCGCGACAT GGCGCGCGAC GCACGGCCCG GAAGGCCCCG CGCCCGACTT GGCGACGAGC ACGCGGCCGC AGCTCGACCG CCTCACGCAA TCGCTGATCG CCGCGCTCGC GCGTGTCGCC CCGCTGCGCG ACGCGCCGGA TTGTCCGTCA CGCCTCGCGC GCAGCGTCTC GAACTGGAAG ACCTTGACGC GCTACGACTC CGGCCGCAAG GACGCGCTCG GCACCGCGCT GTCGCACGTC TGCACGGCGG GCAGCACCAG CGCGGTCGGC ACGCGTTAAC CACGTGAGTA AGATAGGGAT CCATATATAG GGCCCGGGTT ATAATTACCT CAGGTCGACG TCCCATGGTT TTGTATAGAA TTTACGGCTA GCGCCGGATG CGACGCCGGT CGCGTCTTAT CCGGCCTTCC TATATCAGGC GGTGTTTAAG ACGCCGCCGC TTCGCCCAAA TCCTTATGCC GGTTCGACGA CTGGACAAAA TACTGTTTAT CTTCCCAGCG CAGGCAGGTT AATGTACCAC CCCAGCAGCA GCCGGTATCC AGCGCGTATA TACCTTCCGG CGTACCTTTG CCCTCCAGCG ATGCCCAGTG ACCAAAGGCG ATGCTGTATT CTTCAGCGAC AGGGCCAGGA ATCGCAAACC ACGGTTTCAG TGGGGCAGGG GCCTCTTCCG GCGATTCTTA CTAGCTAGTA TGCATAGGTG CTGAAATATA AAGTTTGTGT TTCTAAAACA CACTTGGTAC GTACGATAAC GTACAGTGTT TTTCCCTCCA CTTAAATCGA AGGGTAGTGT CTTGGAGCGC GCGGAGTAAA CATATATGGT TCATATATGT CCGTAGGCAC GTAAAAAAAG CGAGGGATTC GAATTCCCCC GGAACCCCCG GTTGGGGCCC ACGCCTCGAT CGAGCAAAAA AAAAAAAAAA GAAAAAAAAA AAAAAAAAAA GCTTTCCCGC GGCCAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCACTCA CTGACTCGCT GCGCTCGGTC GCTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGCGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCTG GCTAGCGATG ACCCTGCTGA TTGGTTCGCT GACCATTTCC GGGTGCGGGA CGGCGTTACC AGAAACTCAG AAGGTTCGTC CAACCAAACC GACTCTGGCG GCAGTTTACG AGAGAGATGA TAGGGTCTGC TTCAGTAAGC CAGATGCTAC ACAATTAGGC TTGTACATAC TGTCGTTAGA ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC ATACGATTTA GGTGACACTA TA