BupsA.00148.c

Probable site-specific

CENTER ID: BupsA.00148.c
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00148.c.A1.GE30895 Full length( BupsA.00148.c ) 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00148.c.A1.PW30783 Full length( BupsA.00148.c ) 1 339

External Resources

RESOURCE REFERENCE ID
RefSeq: WP_011325413.1
RefSeq: YP_333123.1
UniProt: Q3JTI0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATVSKRESG WMCQVRKKGY PATSKKFATK SEALAWGRMI ESEMDRGLFA SRDDAERTTI ADLLDRYTRD ISPTKRSGGS DMGRAGKLKK QLGAYKLTAL TAPHLVAYRD ARLREVSPQT VVHELNLLNR VLTLATREWG VVVPSGVPKV VKPRKPQGRD RRLHPDEIQA IIDATESPDL RDFIRLAVAT GMRRGELASL RWENIDLDQR TAHLPATKTD TPRTVPLSSV ATAVLQARKD AGYTVPFAVT ANALTRAFCR AVQRARRLYE STCTFLERPV TSNWLIGIRL HDLRHEATSR LFEKGLNPME VASITGHKTL SMLQRYTHLR AEDLAKKLG
NT Sequence
ATGGCGACCG TAAGCAAACG CGAAAGTGGA TGGATGTGCC AAGTTCGCAA AAAGGGCTAC CCTGCCACTT CAAAGAAGTT TGCGACCAAG AGCGAAGCCC TTGCATGGGG CCGCATGATC GAATCGGAGA TGGACCGGGG ATTATTCGCG AGCCGTGACG ATGCGGAGCG AACGACCATC GCGGACCTGC TCGACCGGTA CACCCGCGAC ATATCCCCGA CCAAACGCAG CGGCGGGAGC GACATGGGAC GCGCGGGGAA ACTCAAGAAG CAACTGGGCG CGTACAAACT GACCGCCCTA ACCGCACCCC ATCTCGTCGC CTACCGAGAT GCACGACTCA GAGAGGTGTC ACCGCAAACC GTTGTCCATG AATTGAATCT CCTGAATCGC GTACTGACAT TGGCAACACG TGAATGGGGC GTCGTCGTGC CCAGCGGCGT ACCGAAGGTC GTCAAACCCC GGAAGCCACA AGGCCGCGAC CGCAGACTGC ACCCGGACGA GATACAGGCA ATCATCGACG CAACGGAATC TCCCGACCTG AGGGACTTCA TCCGGCTCGC CGTAGCGACT GGGATGCGCC GAGGTGAGTT GGCGTCGCTG CGTTGGGAGA ACATCGACCT CGATCAACGC ACCGCGCATC TACCTGCGAC GAAAACAGAT ACGCCCCGCA CCGTCCCACT CTCCAGCGTC GCCACAGCCG TTCTACAGGC CCGCAAGGAC GCCGGGTACA CCGTACCCTT TGCCGTCACT GCGAACGCCC TCACGCGGGC ATTCTGCCGT GCTGTGCAGC GTGCAAGAAG GCTTTATGAA TCGACGTGTA CATTTCTGGA ACGTCCCGTT ACCTCGAACT GGCTCATCGG TATCAGATTG CACGACCTCC GCCACGAAGC CACAAGCCGC CTATTCGAAA AGGGCTTAAA CCCGATGGAG GTCGCTAGCA TCACGGGCCA CAAGACGCTA AGCATGCTCC AACGCTACAC CCATCTCCGC GCCGAAGATT TGGCGAAGAA ACTGGGC
Details for BupsA.00148.c.A1.GE30895
HARVESTED ON: 11/23/2010
SEQUENCED ON: 12/6/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MATVSKRESG WMCQVRKKGY PATSKKFATK SEALAWGRMI ESEMDRGLFA SRDDAERTTI ADLLDRYTRD ISPTKRSGGS DMGRAGKLKK QLGAYKLTAL TAPHLVAYRD ARLREVSPQT VVHELNLLNR VLTLATREWG VVVPSGVPKV VKPRKPQGRD RRLHPDEIQA IIDATESPDL RDFIRLAVAT GMRRGELASL RWENIDLDQR TAHLPATKTD TPRTVPLSSV ATAVLQARKD AGYTVPFAVT ANALTRAFCR AVQRARRLYE STCTFLERPV TSNWLIGIRL HDLRHEATSR LFEKGLNPME VASITGHKTL SMLQRYTHLR AEDLAKKL
Validated NT Sequence
ccagtttctt cgccaaatct tcggcgcgga gatgggtgta gcgttggagc atgcttagcg tcttgtggcc cgtgatgcta gcgacctcca tcgggtttaa gcccttttcg aataggcggc ttgtggcttc gtggcggagg tcgtgcaatc tgataccgat gagccagttc gaggtaacgg gacgttccag aaatgtacac gtcgattcat aaagccttct tgcacgctgc acagcacggc agaatgcccg cgtgagggcg ttcgcagtga cggcaaaggg tacggtgtac ccggcgtcct tgcgggcctg tagaacggct gtggcgacgc tggagagtgg gacggtgcgg ggcgtatctg ttttcgtcgc aggtagatgc gcggtgcgtt gatcgaggtc gatgttctcc caacgcagcg acgccaactc acctcggcgc atcccagtcg ctacggcgag ccggatgaag tccctcaggt cgggagattc cgttgcgtcg atgattgcct gtatctcgtc cgggtgcagt ctgcggtcgc ggccttgtgg cttccggggt ttgacgacct tcggtacgcc gctgggcacg acgacgcccc attcacgtgt tgccaatgtc agtacgcgat tcaggagatt caattcatgg acaacggttt gcggtgacac ctctctgagt cgtgcatctc ggtaggcgac gagatggggt gcggttaggg cggtcagttt gtacgcgccc agttgcttct tgagtttccc cgcgcgtccc atgtcgctcc cgccgctgcg tttggtcggg gatatgtcgc gggtgtaccg gtcgagcagg tccgcgatgg tcgttcgctc cgcatcgtca cggctcgcga ataatccccg gtccatctcc gattcgatca tgcggcccca tgcaagggct tcgctcttgg tcgcaaactt ctttgaagtg gcagggtagc cctttttgcg aacttggcac atccatccac tttcgcgttt gcttacggtc gccatcgaac caggaccctg ggtctgagct tccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATVSKRES GWMCQVRKKG YPATSKKFAT KSEALAWGRM IESEMDRGLF ASRDDAERTT IADLLDRYTR DISPTKRSGG SDMGRAGKLK KQLGAYKLTA LTAPHLVAYR DARLREVSPQ TVVHELNLLN RVLTLATREW GVVVPSGVPK VVKPRKPQGR DRRLHPDEIQ AIIDATESPD LRDFIRLAVA TGMRRGELAS LRWENIDLDQ RTAHLPATKT DTPRTVPLSS VATAVLQARK DAGYTVPFAV TANALTRAFC RAVQRARRLY ESTCTFLERP VTSNWLIGIR LHDLRHEATS RLFEKGLNPM EVASITGHKT LSMLQRYTHL RAEDLAKKLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgaccgta agcaaacgcg aaagtggatg gatgtgccaa gttcgcaaaa agggctaccc tgccacttca aagaagtttg cgaccaagag cgaagccctt gcatggggcc gcatgatcga atcggagatg gaccggggat tattcgcgag ccgtgacgat gcggagcgaa cgaccatcgc ggacctgctc gaccggtaca cccgcgacat atccccgacc aaacgcagcg gcgggagcga catgggacgc gcggggaaac tcaagaagca actgggcgcg tacaaactga ccgccctaac cgcaccccat ctcgtcgcct accgagatgc acgactcaga gaggtgtcac cgcaaaccgt tgtccatgaa ttgaatctcc tgaatcgcgt actgacattg gcaacacgtg aatggggcgt cgtcgtgccc agcggcgtac cgaaggtcgt caaaccccgg aagccacaag gccgcgaccg cagactgcac ccggacgaga tacaggcaat catcgacgca acggaatctc ccgacctgag ggacttcatc cggctcgccg tagcgactgg gatgcgccga ggtgagttgg cgtcgctgcg ttgggagaac atcgacctcg atcaacgcac cgcgcatcta cctgcgacga aaacagatac gccccgcacc gtcccactct ccagcgtcgc cacagccgtt ctacaggccc gcaaggacgc cgggtacacc gtaccctttg ccgtcactgc gaacgccctc acgcgggcat tctgccgtgc tgtgcagcgt gcaagaaggc tttatgaatc gacgtgtaca tttctggaac gtcccgttac ctcgaactgg ctcatcggta tcagattgca cgacctccgc cacgaagcca caagccgcct attcgaaaag ggcttaaacc cgatggaggt cgctagcatc acgggccaca agacgctaag catgctccaa cgctacaccc atctccgcgc cgaagatttg gcgaagaaac tgggctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.00148.c.A1.PW30783
PURIFICATION DATe: 3/18/2011
CONCENTRATION: 21mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MATVSKRESG WMCQVRKKGY PATSKKFATK SEALAWGRMI ESEMDRGLFA SRDDAERTTI ADLLDRYTRD ISPTKRSGGS DMGRAGKLKK QLGAYKLTAL TAPHLVAYRD ARLREVSPQT VVHELNLLNR VLTLATREWG VVVPSGVPKV VKPRKPQGRD RRLHPDEIQA IIDATESPDL RDFIRLAVAT GMRRGELASL RWENIDLDQR TAHLPATKTD TPRTVPLSSV ATAVLQARKD AGYTVPFAVT ANALTRAFCR AVQRARRLYE STCTFLERPV TSNWLIGIRL HDLRHEATSR LFEKGLNPME VASITGHKTL SMLQRYTHLR AEDLAKKL
Validated NT Sequence
ccagtttctt cgccaaatct tcggcgcgga gatgggtgta gcgttggagc atgcttagcg tcttgtggcc cgtgatgcta gcgacctcca tcgggtttaa gcccttttcg aataggcggc ttgtggcttc gtggcggagg tcgtgcaatc tgataccgat gagccagttc gaggtaacgg gacgttccag aaatgtacac gtcgattcat aaagccttct tgcacgctgc acagcacggc agaatgcccg cgtgagggcg ttcgcagtga cggcaaaggg tacggtgtac ccggcgtcct tgcgggcctg tagaacggct gtggcgacgc tggagagtgg gacggtgcgg ggcgtatctg ttttcgtcgc aggtagatgc gcggtgcgtt gatcgaggtc gatgttctcc caacgcagcg acgccaactc acctcggcgc atcccagtcg ctacggcgag ccggatgaag tccctcaggt cgggagattc cgttgcgtcg atgattgcct gtatctcgtc cgggtgcagt ctgcggtcgc ggccttgtgg cttccggggt ttgacgacct tcggtacgcc gctgggcacg acgacgcccc attcacgtgt tgccaatgtc agtacgcgat tcaggagatt caattcatgg acaacggttt gcggtgacac ctctctgagt cgtgcatctc ggtaggcgac gagatggggt gcggttaggg cggtcagttt gtacgcgccc agttgcttct tgagtttccc cgcgcgtccc atgtcgctcc cgccgctgcg tttggtcggg gatatgtcgc gggtgtaccg gtcgagcagg tccgcgatgg tcgttcgctc cgcatcgtca cggctcgcga ataatccccg gtccatctcc gattcgatca tgcggcccca tgcaagggct tcgctcttgg tcgcaaactt ctttgaagtg gcagggtagc cctttttgcg aacttggcac atccatccac tttcgcgttt gcttacggtc gccatcgaac caggaccctg ggtctgagct tccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATVSKRES GWMCQVRKKG YPATSKKFAT KSEALAWGRM IESEMDRGLF ASRDDAERTT IADLLDRYTR DISPTKRSGG SDMGRAGKLK KQLGAYKLTA LTAPHLVAYR DARLREVSPQ TVVHELNLLN RVLTLATREW GVVVPSGVPK VVKPRKPQGR DRRLHPDEIQ AIIDATESPD LRDFIRLAVA TGMRRGELAS LRWENIDLDQ RTAHLPATKT DTPRTVPLSS VATAVLQARK DAGYTVPFAV TANALTRAFC RAVQRARRLY ESTCTFLERP VTSNWLIGIR LHDLRHEATS RLFEKGLNPM EVASITGHKT LSMLQRYTHL RAEDLAKKLG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGACCGTA AGCAAACGCG AAAGTGGATG GATGTGCCAA GTTCGCAAAA AGGGCTACCC TGCCACTTCA AAGAAGTTTG CGACCAAGAG CGAAGCCCTT GCATGGGGCC GCATGATCGA ATCGGAGATG GACCGGGGAT TATTCGCGAG CCGTGACGAT GCGGAGCGAA CGACCATCGC GGACCTGCTC GACCGGTACA CCCGCGACAT ATCCCCGACC AAACGCAGCG GCGGGAGCGA CATGGGACGC GCGGGGAAAC TCAAGAAGCA ACTGGGCGCG TACAAACTGA CCGCCCTAAC CGCACCCCAT CTCGTCGCCT ACCGAGATGC ACGACTCAGA GAGGTGTCAC CGCAAACCGT TGTCCATGAA TTGAATCTCC TGAATCGCGT ACTGACATTG GCAACACGTG AATGGGGCGT CGTCGTGCCC AGCGGCGTAC CGAAGGTCGT CAAACCCCGG AAGCCACAAG GCCGCGACCG CAGACTGCAC CCGGACGAGA TACAGGCAAT CATCGACGCA ACGGAATCTC CCGACCTGAG GGACTTCATC CGGCTCGCCG TAGCGACTGG GATGCGCCGA GGTGAGTTGG CGTCGCTGCG TTGGGAGAAC ATCGACCTCG ATCAACGCAC CGCGCATCTA CCTGCGACGA AAACAGATAC GCCCCGCACC GTCCCACTCT CCAGCGTCGC CACAGCCGTT CTACAGGCCC GCAAGGACGC CGGGTACACC GTACCCTTTG CCGTCACTGC GAACGCCCTC ACGCGGGCAT TCTGCCGTGC TGTGCAGCGT GCAAGAAGGC TTTATGAATC GACGTGTACA TTTCTGGAAC GTCCCGTTAC CTCGAACTGG CTCATCGGTA TCAGATTGCA CGACCTCCGC CACGAAGCCA CAAGCCGCCT ATTCGAAAAG GGCTTAAACC CGATGGAGGT CGCTAGCATC ACGGGCCACA AGACGCTAAG CATGCTCCAA CGCTACACCC ATCTCCGCGC CGAAGATTTG GCGAAGAAAC TGGGCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA