BupsA.00147.a

anthranilate synthase component I

CENTER ID: BupsA.00147.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00147.a.A1.PS00109 Full length( BupsA.00147.a ) 1 522

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3714
RefSeq: YP_334940.1
UniProt: Q3JNB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRARAQRHA KPGAPRRSSR RQDRNMTELE FQSLANEGYN RIPLIAEALA DLETPLSLYL KLAQPERGGA NSFLLESVVG GERFGRYSFI GLPAHTLVRT KNGVSEVVTD GQVTETHDGD PFAFIATFQS RFKVAQRPGL PRFCGGLAGY FGYDAVRYIE KKLAHTAPRD DLGLPDIQLL LTEEVAVIDN LAGKLYLIVY ADPTKPEAYT KAKQRLRELK QRLRASVVPP VTSASVRTEI YREFKKDDYL AAVRTAKEYI AAGELMQIQV GQRLTKPYRD NPLSLYRALR SLNPSPYMYY YNFGEFHVVG ASPEILVRQE KRGDDQIVTI RPLAGTRPRG NTPERDAELA TELLNDPKEI AEHVMLIDLA RNDVGRIAEI GSVHVTDKMV IEKYSHVQHI VSSVEGKLKP GVTNYDVLRA TFPAGTLSGA PKVRAMELID ELEPIKRGLY GGAVGYLSFS GEMDLAIAIR TGLIHNGNLY VQAAAGIVAD SVPESEWQET ENKARAVLRA AEQVQDGLDS DF
NT Sequence
GTGCTGCGGG CGCGAGCGCA GCGCCACGCG AAACCCGGCG CGCCGCGCCG ATCGTCCCGA CGACAGGACC GGAACATGAC TGAACTCGAA TTCCAATCGC TTGCCAACGA GGGCTACAAC CGCATTCCGC TCATCGCCGA AGCGCTGGCC GACCTCGAAA CGCCGCTTTC ACTGTATCTG AAGCTCGCGC AGCCCGAACG CGGCGGCGCC AACTCGTTCC TGCTCGAATC GGTGGTGGGC GGCGAGCGCT TCGGACGCTA TTCGTTCATC GGCCTGCCCG CGCATACGCT GGTGCGCACG AAGAACGGCG TGTCGGAGGT CGTGACGGAC GGCCAGGTCA CCGAGACCCA CGACGGCGAC CCGTTCGCGT TCATCGCGAC ATTCCAGAGC CGCTTCAAGG TCGCGCAGCG CCCCGGCCTG CCGCGCTTCT GCGGCGGCCT CGCCGGCTAT TTCGGCTACG ACGCGGTGCG CTACATCGAG AAGAAGCTCG CGCACACCGC GCCGCGCGAC GATCTCGGCC TGCCCGACAT CCAGTTGCTG CTGACCGAGG AAGTCGCCGT GATCGACAAC CTCGCCGGCA AGCTCTACCT GATCGTCTAT GCCGATCCGA CGAAGCCCGA GGCGTACACG AAAGCCAAGC AACGGCTGCG CGAGCTCAAG CAGCGGCTGC GCGCGAGCGT CGTGCCGCCC GTCACGTCGG CGAGCGTGCG CACCGAGATC TATCGCGAAT TCAAGAAGGA TGACTATCTG GCCGCCGTGC GCACGGCGAA GGAATACATC GCGGCGGGCG AGCTGATGCA GATCCAGGTC GGCCAGCGCC TGACGAAGCC GTATCGCGAC AATCCGCTGT CGCTGTACCG CGCGCTGCGC TCGCTGAACC CGTCGCCATA CATGTATTAC TACAATTTCG GCGAATTCCA TGTCGTCGGC GCTTCGCCGG AGATTCTCGT GCGTCAGGAG AAGCGCGGCG ACGACCAGAT CGTGACGATC CGCCCGCTTG CCGGCACGCG GCCGCGGGGC AACACGCCCG AGCGCGACGC CGAGCTCGCG ACCGAACTGC TCAACGATCC GAAGGAAATC GCCGAGCACG TGATGCTGAT CGACCTCGCG CGCAACGACG TCGGCCGCAT CGCGGAAATC GGCTCGGTCC ACGTGACCGA CAAGATGGTG ATCGAGAAAT ACTCGCACGT GCAGCACATC GTGAGTTCGG TCGAGGGCAA GCTGAAGCCC GGCGTGACGA ACTACGACGT GCTGCGCGCG ACGTTCCCGG CGGGCACGCT GTCCGGCGCG CCGAAAGTCC GCGCGATGGA GCTGATCGAC GAGCTCGAGC CGATCAAGCG CGGGCTGTAC GGCGGCGCGG TCGGCTACCT GTCGTTCTCG GGCGAGATGG ATCTCGCGAT CGCGATCCGC ACGGGCCTCA TCCACAACGG CAATCTGTAC GTGCAGGCGG CGGCGGGCAT CGTCGCCGAC TCGGTGCCCG AATCCGAATG GCAGGAGACC GAGAACAAGG CGCGCGCGGT GCTGCGCGCG GCCGAACAGG TGCAAGACGG CCTCGATTCC GATTTC
Details for BupsA.00147.a.A1.PS00109
PURIFICATION DATe: 10/14/2008
CONCENTRATION: 13.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLRARAQR HAKPGAPRRS SRRQDRNMTE LEFQSLANEG YNRIPLIAEA LADLETPLSL YLKLAQPERG GANSFLLESV VGGERFGRYS FIGLPAHTLV RTKNGVSEVV TDGQVTETHD GDPFAFIATF QSRFKVAQRP GLPRFCGGLA GYFGYDAVRY IEKKLAHTAP RDDLGLPDIQ LLLTEEVAVI DNLAGKLYLI VYADPTKPEA YTKAKQRLRE LKQRLRASVV PPVTSASVRT EIYREFKKDD YLAAVRTAKE YIAAGELMQI QVGQRLTKPY RDNPLSLYRA LRSLNPSPYM YYYNFGEFHV VGASPEILVR QEKRGDDQIV TIRPLAGTRP RGNTPERDAE LATELLNDPK EIAEHVMLID LARNDVGRIA EIGSVHVTDK MVIEKYSHVQ HIVSSVEGKL KPGVTNYDVL RATFPAGTLS GAPKVRAMEL IDELEPIKRG LYGGAVGYLS FSGEMDLAIA IRTGLIHNGN LYVQAAAGIV ADSVPESEWQ ETENKARAVL RAAEQVQDGL DSDF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCTGCGG GCGCGAGCGC AGCGCCACGC GAAACCCGGC GCGCCGCGCC GATCGTCCCG ACGACAGGAC CGGAACATGA CTGAACTCGA ATTCCAATCG CTTGCCAACG AGGGCTACAA CCGCATTCCG CTCATCGCCG AAGCGCTGGC CGACCTCGAA ACGCCGCTTT CACTGTATCT GAAGCTCGCG CAGCCCGAAC GCGGCGGCGC CAACTCGTTC CTGCTCGAAT CGGTGGTGGG CGGCGAGCGC TTCGGACGCT ATTCGTTCAT CGGCCTGCCC GCGCATACGC TGGTGCGCAC GAAGAACGGC GTGTCGGAGG TCGTGACGGA CGGCCAGGTC ACCGAGACCC ACGACGGCGA CCCGTTCGCG TTCATCGCGA CATTCCAGAG CCGCTTCAAG GTCGCGCAGC GCCCCGGCCT GCCGCGCTTC TGCGGCGGCC TCGCCGGCTA TTTCGGCTAC GACGCGGTGC GCTACATCGA GAAGAAGCTC GCGCACACCG CGCCGCGCGA CGATCTCGGC CTGCCCGACA TCCAGTTGCT GCTGACCGAG GAAGTCGCCG TGATCGACAA CCTCGCCGGC AAGCTCTACC TGATCGTCTA TGCCGATCCG ACGAAGCCCG AGGCGTACAC GAAAGCCAAG CAACGGCTGC GCGAGCTCAA GCAGCGGCTG CGCGCGAGCG TCGTGCCGCC CGTCACGTCG GCGAGCGTGC GCACCGAGAT CTATCGCGAA TTCAAGAAGG ATGACTATCT GGCCGCCGTG CGCACGGCGA AGGAATACAT CGCGGCGGGC GAGCTGATGC AGATCCAGGT CGGCCAGCGC CTGACGAAGC CGTATCGCGA CAATCCGCTG TCGCTGTACC GCGCGCTGCG CTCGCTGAAC CCGTCGCCAT ACATGTATTA CTACAATTTC GGCGAATTCC ATGTCGTCGG CGCTTCGCCG GAGATTCTCG TGCGTCAGGA GAAGCGCGGC GACGACCAGA TCGTGACGAT CCGCCCGCTT GCCGGCACGC GGCCGCGGGG CAACACGCCC GAGCGCGACG CCGAGCTCGC GACCGAACTG CTCAACGATC CGAAGGAAAT CGCCGAGCAC GTGATGCTGA TCGACCTCGC GCGCAACGAC GTCGGCCGCA TCGCGGAAAT CGGCTCGGTC CACGTGACCG ACAAGATGGT GATCGAGAAA TACTCGCACG TGCAGCACAT CGTGAGTTCG GTCGAGGGCA AGCTGAAGCC CGGCGTGACG AACTACGACG TGCTGCGCGC GACGTTCCCG GCGGGCACGC TGTCCGGCGC GCCGAAAGTC CGCGCGATGG AGCTGATCGA CGAGCTCGAG CCGATCAAGC GCGGGCTGTA CGGCGGCGCG GTCGGCTACC TGTCGTTCTC GGGCGAGATG GATCTCGCGA TCGCGATCCG CACGGGCCTC ATCCACAACG GCAATCTGTA CGTGCAGGCG GCGGCGGGCA TCGTCGCCGA CTCGGTGCCC GAATCCGAAT GGCAGGAGAC CGAGAACAAG GCGCGCGCGG TGCTGCGCGC GGCCGAACAG GTGCAAGACG GCCTCGATTC CGATTTCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA