BupsA.00141.a
acyl-carrier-protein S-malonyltransferase
| CENTER ID: | BupsA.00141.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00141.a.A1.GE25439 | Full length( BupsA.00141.a ) | 1 | 310 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00141.a.A1.PW25477 | Full length( BupsA.00141.a ) | 1 | 310 |
Structures
3EZO
DEPOSITED: 10/23/2008
DETERMINATION: XRay
CLONE: BupsA.00141.a.A1.GE25439
PROTEIN: BupsA.00141.a.A1.PW25477
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127857 |
| PATRIC ID: | fig|320372.6.peg.3037 |
| RefSeq: | YP_334287.1 |
| UniProt: | Q3JQ66 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFAFVFPGQ GSQSVGMLNA FADVAVVRET LDEASDALGQ DIGKLIADGP ADELNLTTNT QPVMLTAAYA CYRAWQQAGG AQPSIVAGHS LGEYTALVAA GAIAFRDALP LVRFRAQAMQ TAVPVGVGGM AAILGLDDDT VRAVCAEASA TGVVEAVNFN APAQVVIAGT KAGIEKACEI AKEKGAKRAL PLPVSAPFHS SLLKPASDKL REYLAGVDVK APKISVVNNI DVAVVSDPAA IKDALVRQAA GPVRWVECVQ HIAREGVTHV IECGPGKVLA GLTKRIDGNL VGASVFDPAS LDEALKLAAA
NT Sequence
ATGAAATTCG CATTCGTTTT TCCGGGCCAG GGCTCGCAAT CGGTCGGCAT GCTCAATGCA TTCGCCGACG TCGCCGTCGT GCGCGAGACG CTCGACGAAG CGTCCGATGC GCTTGGCCAG GATATCGGCA AGCTGATCGC CGACGGCCCG GCCGACGAAC TCAATCTGAC GACGAATACG CAACCCGTGA TGCTGACGGC CGCGTACGCG TGCTATCGCG CGTGGCAGCA GGCGGGCGGC GCGCAGCCGT CGATCGTCGC GGGCCACAGC CTCGGCGAAT ACACGGCGCT CGTCGCCGCG GGCGCGATCG CGTTTCGCGA CGCGCTGCCG CTCGTGCGCT TTCGCGCGCA GGCGATGCAG ACGGCCGTGC CCGTCGGCGT GGGCGGCATG GCGGCGATTC TCGGGCTCGA CGACGACACG GTGCGCGCGG TGTGCGCCGA GGCGTCGGCG ACGGGCGTCG TCGAAGCGGT GAACTTCAAC GCGCCCGCGC AGGTCGTGAT CGCGGGCACG AAGGCGGGCA TCGAGAAGGC GTGCGAGATC GCGAAGGAGA AGGGCGCGAA GCGCGCGCTG CCGCTGCCGG TGTCGGCGCC GTTCCATTCG TCGCTGCTCA AGCCCGCGTC GGACAAGCTG CGCGAATATC TCGCGGGCGT CGACGTGAAG GCGCCGAAGA TTTCCGTCGT CAACAACATC GACGTCGCGG TGGTGTCCGA TCCCGCGGCG ATCAAGGATG CGCTCGTGCG CCAGGCGGCG GGCCCCGTGC GCTGGGTCGA GTGCGTGCAG CACATCGCCC GCGAGGGCGT GACGCACGTG ATCGAGTGCG GTCCGGGCAA GGTGCTCGCC GGCCTCACGA AGCGCATCGA CGGCAATCTG GTCGGCGCGT CGGTGTTCGA TCCGGCGTCG CTCGACGAAG CGCTCAAGCT CGCCGCCGCC
Details for BupsA.00141.a.A1.GE25439
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 3/6/2009 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKFAFVFPG QGSQSVGMLN AFADVAVVRE TLDEASDALG QDIGKLIADG PADELNLTTN TQPVMLTAAY ACYRAWQQAG GAQPSIVAGH SLGEYTALVA AGAIAFRDAL PLVRFRAQAM QTAVPVGVGG MAAILGLDDD TVRAVCAEAS ATGVVEAVNF NAPAQVVIAG TKAGIEKACE IAKEKGAKRA LPLPVSAPFH SSLLKPASDK LREYLAGVDV KAPKISVVNN IDVAVVSDPA AIKDALVRQA AGPVRWVECV QHIAREGVTH VIECGPGKVL AGLTKRIDGN LVGASVFDPA SLDEALKLAA A
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat tcgcattcgt ttttccgggc cagggctcgc aatcggtcgg catgctcaat gcattcgccg acgtcgccgt cgtgcgcgag acgctcgacg aagcgtccga tgcgcttggc caggatatcg gcaagctgat cgccgacggc ccggccgacg aactcaatct gacgacgaat acgcaacccg tgatgctgac ggccgcgtac gcgtgctatc gcgcgtggca gcaggcgggc ggcgcgcagc cgtcgatcgt cgcgggccac agcctcggcg aatacacggc gctcgtcgcc gcgggcgcga tcgcgtttcg cgacgcgctg ccgctcgtgc gctttcgcgc gcaggcgatg cagacggccg tgcccgtcgg cgtgggcggc atggcggcga ttctcgggct cgacgacgac acggtgcgcg cggtgtgcgc cgaggcgtcg gcgacgggcg tcgtcgaagc ggtgaacttc aacgcgcccg cgcaggtcgt gatcgcgggc acgaaggcgg gcatcgagaa ggcgtgcgag atcgcgaagg agaagggcgc gaagcgcgcg ctgccgctgc cggtgtcggc gccgttccat tcgtcgctgc tcaagcccgc gtcggacaag ctgcgcgaat atctcgcggg cgtcgacgtg aaggcgccga agatttccgt cgtcaacaac atcgacgtcg cggtggtgtc cgatcccgcg gcgatcaagg atgcgctcgt gcgccaggcg gcgggccccg tgcgctgggt cgagtgcgtg cagcacatcg cccgcgaggg cgtgacgcac gtgatcgagt gcggtccggg caaggtgctc gccggcctca cgaagcgcat cgacggcaat ctggtcggcg cgtcggtgtt cgatccggcg tcgctcgacg aagcgctcaa gctcgccgcc gccta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFAFVFPG QGSQSVGMLN AFADVAVVRE TLDEASDALG QDIGKLIADG PADELNLTTN TQPVMLTAAY ACYRAWQQAG GAQPSIVAGH SLGEYTALVA AGAIAFRDAL PLVRFRAQAM QTAVPVGVGG MAAILGLDDD TVRAVCAEAS ATGVVEAVNF NAPAQVVIAG TKAGIEKACE IAKEKGAKRA LPLPVSAPFH SSLLKPASDK LREYLAGVDV KAPKISVVNN IDVAVVSDPA AIKDALVRQA AGPVRWVECV QHIAREGVTH VIECGPGKVL AGLTKRIDGN LVGASVFDPA SLDEALKLAA A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaattcgca ttcgtttttc cgggccaggg ctcgcaatcg gtcggcatgc tcaatgcatt cgccgacgtc gccgtcgtgc gcgagacgct cgacgaagcg tccgatgcgc ttggccagga tatcggcaag ctgatcgccg acggcccggc cgacgaactc aatctgacga cgaatacgca acccgtgatg ctgacggccg cgtacgcgtg ctatcgcgcg tggcagcagg cgggcggcgc gcagccgtcg atcgtcgcgg gccacagcct cggcgaatac acggcgctcg tcgccgcggg cgcgatcgcg tttcgcgacg cgctgccgct cgtgcgcttt cgcgcgcagg cgatgcagac ggccgtgccc gtcggcgtgg gcggcatggc ggcgattctc gggctcgacg acgacacggt gcgcgcggtg tgcgccgagg cgtcggcgac gggcgtcgtc gaagcggtga acttcaacgc gcccgcgcag gtcgtgatcg cgggcacgaa ggcgggcatc gagaaggcgt gcgagatcgc gaaggagaag ggcgcgaagc gcgcgctgcc gctgccggtg tcggcgccgt tccattcgtc gctgctcaag cccgcgtcgg acaagctgcg cgaatatctc gcgggcgtcg acgtgaaggc gccgaagatt tccgtcgtca acaacatcga cgtcgcggtg gtgtccgatc ccgcggcgat caaggatgcg ctcgtgcgcc aggcggcggg ccccgtgcgc tgggtcgagt gcgtgcagca catcgcccgc gagggcgtga cgcacgtgat cgagtgcggt ccgggcaagg tgctcgccgg cctcacgaag cgcatcgacg gcaatctggt cggcgcgtcg gtgttcgatc cggcgtcgct cgacgaagcg ctcaagctcg ccgccgccaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.00141.a.A1.PW25477
| PURIFICATION DATe: | 8/18/2008 |
| CONCENTRATION: | 34.11mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 20 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKFAFVFPG QGSQSVGMLN AFADVAVVRE TLDEASDALG QDIGKLIADG PADELNLTTN TQPVMLTAAY ACYRAWQQAG GAQPSIVAGH SLGEYTALVA AGAIAFRDAL PLVRFRAQAM QTAVPVGVGG MAAILGLDDD TVRAVCAEAS ATGVVEAVNF NAPAQVVIAG TKAGIEKACE IAKEKGAKRA LPLPVSAPFH SSLLKPASDK LREYLAGVDV KAPKISVVNN IDVAVVSDPA AIKDALVRQA AGPVRWVECV QHIAREGVTH VIECGPGKVL AGLTKRIDGN LVGASVFDPA SLDEALKLAA A
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat tcgcattcgt ttttccgggc cagggctcgc aatcggtcgg catgctcaat gcattcgccg acgtcgccgt cgtgcgcgag acgctcgacg aagcgtccga tgcgcttggc caggatatcg gcaagctgat cgccgacggc ccggccgacg aactcaatct gacgacgaat acgcaacccg tgatgctgac ggccgcgtac gcgtgctatc gcgcgtggca gcaggcgggc ggcgcgcagc cgtcgatcgt cgcgggccac agcctcggcg aatacacggc gctcgtcgcc gcgggcgcga tcgcgtttcg cgacgcgctg ccgctcgtgc gctttcgcgc gcaggcgatg cagacggccg tgcccgtcgg cgtgggcggc atggcggcga ttctcgggct cgacgacgac acggtgcgcg cggtgtgcgc cgaggcgtcg gcgacgggcg tcgtcgaagc ggtgaacttc aacgcgcccg cgcaggtcgt gatcgcgggc acgaaggcgg gcatcgagaa ggcgtgcgag atcgcgaagg agaagggcgc gaagcgcgcg ctgccgctgc cggtgtcggc gccgttccat tcgtcgctgc tcaagcccgc gtcggacaag ctgcgcgaat atctcgcggg cgtcgacgtg aaggcgccga agatttccgt cgtcaacaac atcgacgtcg cggtggtgtc cgatcccgcg gcgatcaagg atgcgctcgt gcgccaggcg gcgggccccg tgcgctgggt cgagtgcgtg cagcacatcg cccgcgaggg cgtgacgcac gtgatcgagt gcggtccggg caaggtgctc gccggcctca cgaagcgcat cgacggcaat ctggtcggcg cgtcggtgtt cgatccggcg tcgctcgacg aagcgctcaa gctcgccgcc gccta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFAFVFPG QGSQSVGMLN AFADVAVVRE TLDEASDALG QDIGKLIADG PADELNLTTN TQPVMLTAAY ACYRAWQQAG GAQPSIVAGH SLGEYTALVA AGAIAFRDAL PLVRFRAQAM QTAVPVGVGG MAAILGLDDD TVRAVCAEAS ATGVVEAVNF NAPAQVVIAG TKAGIEKACE IAKEKGAKRA LPLPVSAPFH SSLLKPASDK LREYLAGVDV KAPKISVVNN IDVAVVSDPA AIKDALVRQA AGPVRWVECV QHIAREGVTH VIECGPGKVL AGLTKRIDGN LVGASVFDPA SLDEALKLAA A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATTCGCA TTCGTTTTTC CGGGCCAGGG CTCGCAATCG GTCGGCATGC TCAATGCATT CGCCGACGTC GCCGTCGTGC GCGAGACGCT CGACGAAGCG TCCGATGCGC TTGGCCAGGA TATCGGCAAG CTGATCGCCG ACGGCCCGGC CGACGAACTC AATCTGACGA CGAATACGCA ACCCGTGATG CTGACGGCCG CGTACGCGTG CTATCGCGCG TGGCAGCAGG CGGGCGGCGC GCAGCCGTCG ATCGTCGCGG GCCACAGCCT CGGCGAATAC ACGGCGCTCG TCGCCGCGGG CGCGATCGCG TTTCGCGACG CGCTGCCGCT CGTGCGCTTT CGCGCGCAGG CGATGCAGAC GGCCGTGCCC GTCGGCGTGG GCGGCATGGC GGCGATTCTC GGGCTCGACG ACGACACGGT GCGCGCGGTG TGCGCCGAGG CGTCGGCGAC GGGCGTCGTC GAAGCGGTGA ACTTCAACGC GCCCGCGCAG GTCGTGATCG CGGGCACGAA GGCGGGCATC GAGAAGGCGT GCGAGATCGC GAAGGAGAAG GGCGCGAAGC GCGCGCTGCC GCTGCCGGTG TCGGCGCCGT TCCATTCGTC GCTGCTCAAG CCCGCGTCGG ACAAGCTGCG CGAATATCTC GCGGGCGTCG ACGTGAAGGC GCCGAAGATT TCCGTCGTCA ACAACATCGA CGTCGCGGTG GTGTCCGATC CCGCGGCGAT CAAGGATGCG CTCGTGCGCC AGGCGGCGGG CCCCGTGCGC TGGGTCGAGT GCGTGCAGCA CATCGCCCGC GAGGGCGTGA CGCACGTGAT CGAGTGCGGT CCGGGCAAGG TGCTCGCCGG CCTCACGAAG CGCATCGACG GCAATCTGGT CGGCGCGTCG GTGTTCGATC CGGCGTCGCT CGACGAAGCG CTCAAGCTCG CCGCCGCCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA