SSGCID: Target Page

BupsA.00138.b

putative alanyl-tRNA synthetase related protein

CENTER ID:	BupsA.00138.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00138.b.A1.GE25437	Full length( BupsA.00138.b )		1	239

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.4328
RefSeq:	YP_335495.1
UniProt:	Q3JLQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNRLFCQDDP DTLLLSGTVV DTRPGAVRLS RHPFYPGGGG QLPDLGTVRW EGGVETVAGF SEDGGGLWIE FANGVEIVGD VQSSVDAAFR RAMRELHTDL HVLNALVYRD FDGALVTGVQ MNADRTARID FDLPDADNDR LRALEAEMND VVRRDLPVRT RYVPLADAQQ ASGLLRSRSV APPPTADGLV RIVEIDGLDS QACGGTHLAS TGGSSPVRIL KIENKGRSNR RVRIGLEPR

NT Sequence

ATGAATCGAT TGTTCTGTCA AGACGACCCC GACACGCTGC TGCTTTCGGG CACGGTGGTC GACACGCGGC CGGGCGCGGT GCGCTTGTCG CGGCATCCGT TCTATCCGGG CGGCGGCGGC CAGTTGCCCG ATCTGGGCAC GGTTCGATGG GAAGGCGGCG TCGAGACCGT CGCCGGTTTC AGCGAGGACG GCGGCGGCCT CTGGATCGAA TTCGCGAACG GCGTCGAGAT CGTCGGCGAC GTGCAGTCGA GCGTCGACGC CGCGTTCCGC CGCGCGATGC GCGAGCTGCA TACCGATCTG CACGTGCTCA ACGCGCTCGT CTATCGCGAT TTCGACGGCG CGCTCGTGAC GGGCGTGCAG ATGAACGCCG ATCGCACCGC GCGCATCGAT TTCGATCTGC CCGACGCCGA TAACGATCGC CTGCGCGCGC TGGAGGCCGA AATGAACGAT GTCGTGCGCC GCGATCTGCC GGTGCGCACG CGCTACGTGC CGCTCGCCGA CGCGCAGCAG GCAAGCGGGC TGCTGCGCTC GCGCTCGGTC GCGCCGCCGC CGACGGCCGA CGGGCTCGTG CGGATCGTCG AGATCGACGG GCTCGACAGC CAGGCGTGCG GCGGCACGCA TCTCGCGTCG ACGGGCGGCT CGAGCCCGGT GCGGATCCTG AAGATCGAAA ACAAGGGCCG CTCGAACCGC CGCGTGCGCA TCGGGCTGGA GCCGAGA

Details for BupsA.00138.b.A1.GE25437

HARVESTED ON:	6/30/2008
SEQUENCED ON:	3/16/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNRLFCQDD PDTLLLSGTV VDTRPGAVRL SRHPFYPGGG GQLPDLGTVR WEGGVETVAG FSEDGGGLWI EFANGVEIVG DVQSSVDAAF RRAMRELHTD LHVLNALVYR DFDGALVTGV QMNADRTARI DFDLPDADND RLRALEAEMN DVVRRDLPVR TRYVPLADAQ QASGLLRSRS VAPPPTADGL VRIVEIDGLD SQACGGTHLA STGGSSPVRI LKIENKGRSN RRVRIGLEPR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaatc gattgttctg tcaagacgac cccgacacgc tgctgctttc gggcacggtg gtcgacacgc ggccgggcgc ggtgcgcttg tcgcggcatc cgttctatcc gggcggcggc ggccagttgc ccgatctggg cacggttcga tgggaaggcg gcgtcgagac cgtcgccggt ttcagcgagg acggcggcgg cctctggatc gaattcgcga acggcgtcga gatcgtcggc gacgtgcagt cgagcgtcga cgccgcgttc cgccgcgcga tgcgcgagct gcataccgat ctgcacgtgc tcaacgcgct cgtctatcgc gatttcgacg gcgcgctcgt gacgggcgtg cagatgaacg ccgatcgcac cgcgcgcatc gatttcgatc tgcccgacgc cgataacgat cgcctgcgcg cgctggaggc cgaaatgaac gatgtcgtgc gccgcgatct gccggtgcgc acgcgctacg tgccgctcgc cgacgcgcag caggcaagcg ggctgctgcg ctcgcgctcg gtcgcgccgc cgccgacggc cgacgggctc gtgcggatcg tcgagatcga cgggctcgac agccaggcgt gcggcggcac gcatctcgcg tcgacgggcg gctcgagccc ggtgcggatc ctgaagatcg aaaacaaggg ccgctcgaac cgccgcgtgc gcatcgggct ggagccgaga taataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatcgattg ttctgtcaag acgaccccga cacgctgctg ctttcgggca cggtggtcga cacgcggccg ggcgcggtgc gcttgtcgcg gcatccgttc tatccgggcg gcggcggcca gttgcccgat ctgggcacgg ttcgatggga aggcggcgtc gagaccgtcg ccggtttcag cgaggacggc ggcggcctct ggatcgaatt cgcgaacggc gtcgagatcg tcggcgacgt gcagtcgagc gtcgacgccg cgttccgccg cgcgatgcgc gagctgcata ccgatctgca cgtgctcaac gcgctcgtct atcgcgattt cgacggcgcg ctcgtgacgg gcgtgcagat gaacgccgat cgcaccgcgc gcatcgattt cgatctgccc gacgccgata acgatcgcct gcgcgcgctg gaggccgaaa tgaacgatgt cgtgcgccgc gatctgccgg tgcgcacgcg ctacgtgccg ctcgccgacg cgcagcaggc aagcgggctg ctgcgctcgc gctcggtcgc gccgccgccg acggccgacg ggctcgtgcg gatcgtcgag atcgacgggc tcgacagcca ggcgtgcggc ggcacgcatc tcgcgtcgac gggcggctcg agcccggtgc ggatcctgaa gatcgaaaac aagggccgct cgaaccgccg cgtgcgcatc gggctggagc cgagaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa