BupsA.00138.a

alanyl-tRNA synthetase

CENTER ID: BupsA.00138.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00138.a.A1.GE25436 Full length( BupsA.00138.a ) 1 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1446
RefSeq: YP_332812.1
UniProt: Q3JUE1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATQALFRED AYLTRCDALV TAVDAAGIHL DRTVFYPLGG GQAGDSGTLT LAGGAALAVA DTRKAKFDGA TPDDAVHVLA PGQAALAAGV KPGDALVAEI DWAPRHRRMR LHTASHLLCA VLPYPVDGCS VTADYVRIDF ATVEPIDREH VQARLAELVG GAHPVTTEWI TDDEMVARPE LVRTMSVKPP MGLGRVRLLR IEGVDLQPCG GTHVRNTAEI GALRVAKLEK KSARTRRLVL ELA
NT Sequence
ATGGCCACTC AAGCTCTCTT TCGCGAAGAC GCTTATCTGA CGCGTTGCGA CGCGCTCGTC ACCGCCGTCG ACGCAGCCGG CATCCATCTC GATCGAACCG TGTTCTACCC GCTCGGCGGC GGCCAGGCGG GCGACAGCGG CACGCTGACG CTCGCGGGCG GCGCGGCGCT CGCCGTCGCC GACACGCGCA AGGCGAAGTT CGACGGCGCG ACGCCCGACG ACGCGGTGCA CGTGCTCGCG CCGGGGCAGG CGGCGCTCGC GGCGGGCGTG AAGCCGGGCG ATGCGCTCGT CGCCGAAATC GATTGGGCGC CGCGGCACCG CCGGATGCGG CTGCACACGG CGAGCCATCT GCTGTGCGCG GTGCTGCCGT ATCCGGTCGA CGGCTGCAGC GTGACCGCCG ATTACGTTCG GATCGATTTC GCGACGGTCG AGCCGATCGA TCGCGAGCAT GTGCAGGCGC GACTCGCCGA GCTCGTCGGC GGCGCGCATC CGGTGACGAC CGAATGGATC ACCGACGACG AAATGGTCGC GCGCCCGGAG CTCGTGCGCA CGATGAGCGT GAAGCCGCCA ATGGGGCTCG GGCGCGTGCG GCTGCTGCGG ATCGAAGGCG TCGACCTGCA GCCGTGCGGT GGCACGCACG TGCGCAACAC GGCGGAGATC GGCGCGCTGC GCGTCGCGAA GCTCGAGAAG AAGAGCGCGC GCACGCGCCG TCTCGTGCTG GAGCTCGCA
Details for BupsA.00138.a.A1.GE25436
HARVESTED ON: 6/30/2008
SEQUENCED ON: 3/6/2009
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMATQALFXE DAYLTRCDAL VTAVDAAGIH LDRTVFYPLG GGQAGDSGTL TLAGGAALAV ADTRKAKFDG ATPDDAVHVL APGQAALAAG VKPGDALVAE IDWAPRHRRM RLHTASHLLC AVLPYPVDGC SVTADYVRID FATVEPIDRE HVQARLAELV GGAHPVTTEW ITDDEMVARP ELVRTMSVKP PMGLGRVRLL RIEGVDLQPC GGTHVRNTAE IGALRVAKLE KKSARTRRLV LEL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcca ctcaagctct ctttcncgaa gacgcttatc tgacgcgttg cgacgcgctc gtcaccgccg tcgacgcagc cggcatccat ctcgatcgaa ccgtgttcta cccgctcggc ggcggccagg cgggcgacag cggcacgctg acgctcgcgg gcggcgcggc gctcgccgtc gccgacacgc gcaaggcgaa gttcgacggc gcgacgcccg acgacgcggt gcacgtgctc gcgccggggc aggcggcgct cgcggcgggc gtgaagccgg gcgatgcgct cgtcgccgaa atcgattggg cgccgcggca ccgccggatg cggctgcaca cggcgagcca tctgctgtgc gcggtgctgc cgtatccggt cgacggctgc agcgtgaccg ccgattacgt tcggatcgat ttcgcgacgg tcgagccgat cgatcgcgag catgtgcagg cgcgactcgc cgagctcgtc ggcggcgcgc atccggtgac gaccgaatgg atcaccgacg acgaaatggt cgcgcgcccg gagctcgtgc gcacgatgag cgtgaagccg ccaatggggc tcgggcgcgt gcggctgctg cggatcgaag gcgtcgacct gcagccgtgc ggtggcacgc acgtgcgcaa cacggcggag atcggcgcgc tgcgcgtcgc gaagctcgag aagaagagcg cgcgcacgcg ccgtctcgtg ctggagctcg c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATQALFRE DAYLTRCDAL VTAVDAAGIH LDRTVFYPLG GGQAGDSGTL TLAGGAALAV ADTRKAKFDG ATPDDAVHVL APGQAALAAG VKPGDALVAE IDWAPRHRRM RLHTASHLLC AVLPYPVDGC SVTADYVRID FATVEPIDRE HVQARLAELV GGAHPVTTEW ITDDEMVARP ELVRTMSVKP PMGLGRVRLL RIEGVDLQPC GGTHVRNTAE IGALRVAKLE KKSARTRRLV LELA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccactcaa gctctctttc gcgaagacgc ttatctgacg cgttgcgacg cgctcgtcac cgccgtcgac gcagccggca tccatctcga tcgaaccgtg ttctacccgc tcggcggcgg ccaggcgggc gacagcggca cgctgacgct cgcgggcggc gcggcgctcg ccgtcgccga cacgcgcaag gcgaagttcg acggcgcgac gcccgacgac gcggtgcacg tgctcgcgcc ggggcaggcg gcgctcgcgg cgggcgtgaa gccgggcgat gcgctcgtcg ccgaaatcga ttgggcgccg cggcaccgcc ggatgcggct gcacacggcg agccatctgc tgtgcgcggt gctgccgtat ccggtcgacg gctgcagcgt gaccgccgat tacgttcgga tcgatttcgc gacggtcgag ccgatcgatc gcgagcatgt gcaggcgcga ctcgccgagc tcgtcggcgg cgcgcatccg gtgacgaccg aatggatcac cgacgacgaa atggtcgcgc gcccggagct cgtgcgcacg atgagcgtga agccgccaat ggggctcggg cgcgtgcggc tgctgcggat cgaaggcgtc gacctgcagc cgtgcggtgg cacgcacgtg cgcaacacgg cggagatcgg cgcgctgcgc gtcgcgaagc tcgagaagaa gagcgcgcgc acgcgccgtc tcgtgctgga gctcgcaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa