BupsA.00137.b

UDP-N-acetylmuramate--L-alanine ligase

CENTER ID:	BupsA.00137.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00137.b.A1.GE25435	Full length( BupsA.00137.b )		1	465
BupsA.00137.b.B1.GE25531	Full length( BupsA.00137.b )		1	465

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00137.b.B1.PW25574	Full length( BupsA.00137.b )		1	465

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132146
PATRIC ID:	fig\|320372.6.peg.3688
RefSeq:	YP_334914.1
UniProt:	Q3JND9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKHIVKHIHF VGIGGAGMSG IAEVLVNLGY QVSGSDLARN AVTERLEALG ARVSIGHDAA NIEGANAVVV STAVRSDNPE VLAARRLRVP IVPRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLTSAGANAR LGTGDFIVAE ADESDASFLN LYPVIEVITN IDADHMDTYG HDFARLKQAF IEFTQRLPFY GSAVVCIDDA NVRQIVPLIS KPVVRYGFAA DAQVRAENVE ARDGRMHFTV RREGREPLPV VLNLPGLHNV QNALAAIAIA TDLDVADAAI QQALAEFNGV GRRFQRYGEI AAAGGGAYTL IDDYGHHPVE MAATIAAARG AFPGRRLVLA FQPHRYTRTR DCFDDFVNVL STVDALVLTE VYAAGEAPIS TANGDALSRA LRAAGKVEPV FVATVDEVPD ALAKLARDGD VVITMGAGSI GGVPGKLAQD TQQKG

NT Sequence

ATGAAACACA TCGTCAAACA CATTCACTTC GTCGGGATCG GCGGCGCGGG CATGAGCGGC ATCGCCGAAG TGCTCGTGAA CCTGGGCTAC CAGGTGAGCG GCTCCGATCT CGCGCGCAAC GCGGTGACCG AGCGCCTCGA GGCGCTCGGC GCGCGCGTGT CGATCGGCCA CGATGCGGCG AACATCGAAG GCGCGAACGC GGTCGTCGTG TCGACCGCGG TGCGCTCGGA CAACCCGGAA GTGCTCGCCG CGCGCCGCCT GCGCGTGCCG ATCGTGCCGC GCGCGGTGAT GCTCGCGGAG CTGATGCGCC TGAAGCAGGG GATCGCGATC GCCGGCACGC ACGGCAAGAC GACGACGACG AGCCTCGTCG CGAGCGTGCT CGCCGCGGGC GGGCTCGATC CGACGTTCGT GATCGGCGGG CGTCTCACGA GCGCCGGCGC GAACGCGCGG CTCGGCACGG GCGACTTCAT CGTCGCGGAA GCCGACGAGT CGGACGCGTC ATTCCTGAAC CTGTATCCGG TGATCGAGGT CATCACGAAC ATCGACGCCG ATCACATGGA TACCTACGGC CACGACTTCG CGCGGCTCAA GCAGGCGTTC ATCGAATTCA CGCAGCGGCT GCCGTTCTAC GGCAGCGCGG TCGTGTGCAT CGACGACGCG AACGTGCGGC AGATCGTGCC GCTGATCTCG AAGCCCGTCG TGCGCTACGG CTTCGCGGCG GATGCGCAGG TGCGCGCGGA GAACGTCGAG GCGCGCGACG GCCGGATGCA CTTCACGGTG CGGCGCGAGG GGCGCGAGCC GCTGCCTGTC GTCCTGAACC TGCCCGGCCT GCACAACGTG CAGAACGCGC TCGCGGCGAT CGCGATCGCG ACCGATCTCG ATGTGGCCGA CGCGGCGATC CAGCAGGCGC TCGCGGAATT CAACGGCGTC GGCCGGCGCT TCCAGCGCTA CGGCGAGATC GCGGCGGCGG GCGGCGGCGC GTATACGCTG ATCGACGACT ACGGCCACCA TCCGGTCGAG ATGGCGGCGA CGATCGCGGC GGCGCGCGGC GCGTTCCCGG GCCGCCGGCT CGTGCTCGCG TTCCAGCCGC ATCGCTACAC GCGCACGCGC GACTGCTTCG ACGACTTCGT CAACGTGCTG TCGACGGTCG ACGCGCTCGT GCTGACCGAG GTGTACGCGG CGGGCGAGGC GCCGATTTCG ACCGCGAACG GCGACGCGCT GTCGCGCGCG CTGCGCGCGG CGGGCAAGGT CGAGCCGGTA TTCGTCGCGA CGGTCGACGA GGTGCCGGAC GCGCTCGCGA AGCTCGCGCG CGACGGCGAC GTGGTGATCA CGATGGGCGC GGGCTCGATC GGCGGCGTGC CGGGCAAGCT CGCGCAGGAC ACGCAACAGA AGGGA

Details for BupsA.00137.b.A1.GE25435

HARVESTED ON:	6/30/2008
SEQUENCED ON:	3/16/2012
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKHIVKHIH FVGIGGAGMS GIAEVLVNLG YQVSGSDLAR NAVTERLEAL GARVSIGHDA ANIEGANAVV VSTAVRSDNP EVLAARRLRV PIVPRAVMLA ELMRLKQGIA IAGTHGKTTT TSLVASVLAA GGLDPTFVIG GRLTSAGANA RLGTGDFIVA EADESDASFL NLYPVIEVIT NIDADHMDTY GHDFARLKQA FIEFTQRLPF YGSAVVCIDD ANVRQIVPLI SKPVVRYGFA ADAQVRAENV EARDGRMHFT VRREGREPLP VVLNLPXLHN VQNALAAIAI ATDLDVADAA IQQALAEFNG VGRRFQRYGE IAAAGGGAYT LIDDYGHHPV EMAATIAAAR GAFPGRRLVL AFQPHRYTRT RDCFDDFVNV LSTVDALVLT EVYAAGEAPI STANGDALSR ALRAAGKVEP VFVATVDEVP DALAKLARDG DVVITMGAGS IGGVPGKLAQ DTQQ

Validated NT Sequence

ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaaacac atcgtcaaac acattcactt cgtcgggatc ggcggcgcgg gcatgagcgg catcgccgaa gtgctcgtga acctgggcta ccaggtgagc ggctccgatc tcgcgcgcaa cgcggtgacc gagcgcctcg aggcgctcgg cgcgcgcgtg tcgatcggcc acgatgcggc gaacatcgaa ggcgcgaacg cggtcgtcgt gtcgaccgcg gtgcgctcgg acaacccgga agtgctcgcc gcgcgccgcc tgcgcgtgcc gatcgtgccg cgcgcggtga tgctcgcgga gctgatgcgc ctgaagcagg ggatcgcgat cgccggcacg cacggcaaga cgacgacgac gagcctcgtc gcgagcgtgc tcgccgcggg cgggctcgat ccgacgttcg tgatcggcgg gcgtctcacg agcgccggcg cgaacgcgcg gctcggcacg ggcgacttca tcgtcgcgga agccgacgag tcggacgcgt cattcctgaa cctgtatccg gtgatcgagg tcatcacgaa catcgacgcc gatcacatgg atacctacgg ccacgacttc gcgcggctca agcaggcgtt catcgaattc acgcagcggc tgccgttcta cggcagcgcg gtcgtgtgca tcgacgacgc gaacgtgcgg cagatcgtgc cgctgatctc gaagcccgtc gtgcgctacg gcttcgcggc ggatgcgcag gtgcgcgcgg agaacgtcga ggcgcgcgac ggccggatgc acttcacggt gcggcgcgag gggcgcgagc cgctgcctgt cgtcctgaac ctgcccgncc tgcacaacgt gcagaacgcg ctcgcggcga tcgcgatcgc gaccgatctc gatgtggcng acgcggcgat ccagcaggcg ctcgcggaat tcaacggcgt cggccggcgc ttccagcgct acggcgagat cgcggcggcg ggcggcggcg cgtatacgct gatcgacgac tacggccacc atccggtcga gatggcggcg acgatcgcgg cggcgcgcgg cgcgttcccg ggccgccggc tcgtgctcgc gttccagccg catcgctaca cgcgcacgcg cgactgcttc gacgacttcg tcaacgtgct gtcgacggtc gacgcgctcg tgctgaccga ggtgtacgcg gcgggcgagg cgccgatttc gaccgcgaac ggcgacgcgc tgtcgcgcgc gctgcgcgcg gcgggcaagg tcgagccggt attcgtcgcg acggtcgacg aggtgccgga cgcgctcgcg aagctcgcgc gcgacggcga cgtggtgatc acgatgggcg cgggctcgat cggcggcgtg ccgggcaagc tcgcgcagga cacgcaacag a

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKHIVKHIH FVGIGGAGMS GIAEVLVNLG YQVSGSDLAR NAVTERLEAL GARVSIGHDA ANIEGANAVV VSTAVRSDNP EVLAARRLRV PIVPRAVMLA ELMRLKQGIA IAGTHGKTTT TSLVASVLAA GGLDPTFVIG GRLTSAGANA RLGTGDFIVA EADESDASFL NLYPVIEVIT NIDADHMDTY GHDFARLKQA FIEFTQRLPF YGSAVVCIDD ANVRQIVPLI SKPVVRYGFA ADAQVRAENV EARDGRMHFT VRREGREPLP VVLNLPGLHN VQNALAAIAI ATDLDVADAA IQQALAEFNG VGRRFQRYGE IAAAGGGAYT LIDDYGHHPV EMAATIAAAR GAFPGRRLVL AFQPHRYTRT RDCFDDFVNV LSTVDALVLT EVYAAGEAPI STANGDALSR ALRAAGKVEP VFVATVDEVP DALAKLARDG DVVITMGAGS IGGVPGKLAQ DTQQKG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacacatc gtcaaacaca ttcacttcgt cgggatcggc ggcgcgggca tgagcggcat cgccgaagtg ctcgtgaacc tgggctacca ggtgagcggc tccgatctcg cgcgcaacgc ggtgaccgag cgcctcgagg cgctcggcgc gcgcgtgtcg atcggccacg atgcggcgaa catcgaaggc gcgaacgcgg tcgtcgtgtc gaccgcggtg cgctcggaca acccggaagt gctcgccgcg cgccgcctgc gcgtgccgat cgtgccgcgc gcggtgatgc tcgcggagct gatgcgcctg aagcagggga tcgcgatcgc cggcacgcac ggcaagacga cgacgacgag cctcgtcgcg agcgtgctcg ccgcgggcgg gctcgatccg acgttcgtga tcggcgggcg tctcacgagc gccggcgcga acgcgcggct cggcacgggc gacttcatcg tcgcggaagc cgacgagtcg gacgcgtcat tcctgaacct gtatccggtg atcgaggtca tcacgaacat cgacgccgat cacatggata cctacggcca cgacttcgcg cggctcaagc aggcgttcat cgaattcacg cagcggctgc cgttctacgg cagcgcggtc gtgtgcatcg acgacgcgaa cgtgcggcag atcgtgccgc tgatctcgaa gcccgtcgtg cgctacggct tcgcggcgga tgcgcaggtg cgcgcggaga acgtcgaggc gcgcgacggc cggatgcact tcacggtgcg gcgcgagggg cgcgagccgc tgcctgtcgt cctgaacctg cccggcctgc acaacgtgca gaacgcgctc gcggcgatcg cgatcgcgac cgatctcgat gtggccgacg cggcgatcca gcaggcgctc gcggaattca acggcgtcgg ccggcgcttc cagcgctacg gcgagatcgc ggcggcgggc ggcggcgcgt atacgctgat cgacgactac ggccaccatc cggtcgagat ggcggcgacg atcgcggcgg cgcgcggcgc gttcccgggc cgccggctcg tgctcgcgtt ccagccgcat cgctacacgc gcacgcgcga ctgcttcgac gacttcgtca acgtgctgtc gacggtcgac gcgctcgtgc tgaccgaggt gtacgcggcg ggcgaggcgc cgatttcgac cgcgaacggc gacgcgctgt cgcgcgcgct gcgcgcggcg ggcaaggtcg agccggtatt cgtcgcgacg gtcgacgagg tgccggacgc gctcgcgaag ctcgcgcgcg acggcgacgt ggtgatcacg atgggcgcgg gctcgatcgg cggcgtgccg ggcaagctcg cgcaggacac gcaacagaag ggaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BupsA.00137.b.B1.GE25531

HARVESTED ON:	7/11/2008
SEQUENCED ON:	7/28/2010
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	97

Validated AA Sequence

MKHIVKHIHF VGIGGAGMSG IAEVLVNLGY QVSGSDLARN AVTERLEALG ARVSIGHDAA NIEGANAVVV STAVRSDNPE VLAARRLRVP IVPRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLTSAGANAR LGTGDFIVAE ADESDASFLN LYPVIEVITN IDADHMDTYG HDFARLKQAF IEFTQRLPFY GSAVVCIDDA NVRQIVPLIS KPVVRYGFAA DAQVRAENVE ARDGRMXFTV RREGREPLPV VLNLPGLHNV QNALAAIAIA TDLDVXXAAI QQALAEFNGV GRRFXRXGXI AAAGGGAYXL IDDYGHHPVE MAATIAAARG AXPGRRLVLA XQPHRYTRTR DCFDDFVNVL STVDALVLTE VYAAGEAPIS TANGDALSRA LRAAGKVXPV FVATVDEVPD ALAKLARDGX XVITMGAGSI GGVPGKLAQ

Validated NT Sequence

tggctcacca ccaccaccac catatgaaac acatcgtcaa acacattcac ttcgtcggga tcggcggcgc gggcatgagc ggcatcgccg aagtgctcgt gaacctgggc taccaggtga gcggctccga tctcgcgcgc aacgcggtga ccgagcgcct cgaggcgctc ggcgcgcgcg tgtcgatcgg ccacgatgcg gcgaacatcg aaggcgcgaa cgcggtcgtc gtgtcgaccg cggtgcgctc ggacaacccg gaagtgctcg ccgcgcgccg cctgcgcgtg ccgatcgtgc cgcgcgcggt gatgctcgcg gagctgatgc gcctgaagca ggggatcgcg atcgccggca cgcacggcaa gacgacgacg acgagcctcg tcgcgagcgt gctcgccgcg ggcgggctcg atccgacgtt cgtgatcggc gggcgtctca cgagcgccgg cgcgaacgcg cggctcggca cgggcgactt catcgtcgcg gaagccgacg agtcggacgc gtcattcctg aacctgtatc cggtgatcga ggtcatcacg aacatcgacg ccgatcacat ggatacctac ggccacgact tcgcgcggct caagcaggcg ttcatcgaat tcacgcagcg gctgccgttc tacggcagcg cggtcgtgtg catcgacgac gcgaacgtgc ggcagatcgt gccgctgatc tcgaagcccg tcgtgcgcta cggcttcgcg gcggatgcgc aggtgcgcgc ggagaacgtc gaggcgcgcg acggccggat gnacttcacg gtgcggcgcg aggggcgcga gccgctgcct gtcgtcctga acctgcccgg cctgcacaac gtgcagaacg cgctcgcggc gatcgcgatc gcgaccgatc tcgatgtggn nnncgcggcg atccagcagg cgctcgcgga attcaacggc gtcggccggc gcttcnagcg ctanggcgan atcgcggcgg cgggcggcgg cgcgtatncg ctgatcgacg actacggcca ccatccggtc gagatggcgg cgacgatcgc ggcggcgcgc ggcgcgtncc cgggccgccg gctcgtgctc gcgtnccagc cgcatcgcta cacgcgcacg cgcgactgct tcgacgactt cgtcaacgtg ctgtcgacgg tcgacgcgct cgtgctgacc gaggtgtacg cggcgggcga ggcgccgatt tcgaccgcga acggcgacgc gctgtcgcgc gcgctgcgcg cggcgggcaa ggtcganccg gtattcgtcg cgacggtcga cgaggtgccg gacgcgctcg cgaagctcgc gcgcgacggc nnnntggtga tcacgatggg cgcgggctcg atcggcggcg tnccgggcaa gctcgcgcag gac

Expected Protein Sequence

MAHHHHHHMK HIVKHIHFVG IGGAGMSGIA EVLVNLGYQV SGSDLARNAV TERLEALGAR VSIGHDAANI EGANAVVVST AVRSDNPEVL AARRLRVPIV PRAVMLAELM RLKQGIAIAG THGKTTTTSL VASVLAAGGL DPTFVIGGRL TSAGANARLG TGDFIVAEAD ESDASFLNLY PVIEVITNID ADHMDTYGHD FARLKQAFIE FTQRLPFYGS AVVCIDDANV RQIVPLISKP VVRYGFAADA QVRAENVEAR DGRMHFTVRR EGREPLPVVL NLPGLHNVQN ALAAIAIATD LDVADAAIQQ ALAEFNGVGR RFQRYGEIAA AGGGAYTLID DYGHHPVEMA ATIAAARGAF PGRRLVLAFQ PHRYTRTRDC FDDFVNVLST VDALVLTEVY AAGEAPISTA NGDALSRALR AAGKVEPVFV ATVDEVPDAL AKLARDGDVV ITMGAGSIGG VPGKLAQDTQ QKG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac acatcgtcaa acacattcac ttcgtcggga tcggcggcgc gggcatgagc ggcatcgccg aagtgctcgt gaacctgggc taccaggtga gcggctccga tctcgcgcgc aacgcggtga ccgagcgcct cgaggcgctc ggcgcgcgcg tgtcgatcgg ccacgatgcg gcgaacatcg aaggcgcgaa cgcggtcgtc gtgtcgaccg cggtgcgctc ggacaacccg gaagtgctcg ccgcgcgccg cctgcgcgtg ccgatcgtgc cgcgcgcggt gatgctcgcg gagctgatgc gcctgaagca ggggatcgcg atcgccggca cgcacggcaa gacgacgacg acgagcctcg tcgcgagcgt gctcgccgcg ggcgggctcg atccgacgtt cgtgatcggc gggcgtctca cgagcgccgg cgcgaacgcg cggctcggca cgggcgactt catcgtcgcg gaagccgacg agtcggacgc gtcattcctg aacctgtatc cggtgatcga ggtcatcacg aacatcgacg ccgatcacat ggatacctac ggccacgact tcgcgcggct caagcaggcg ttcatcgaat tcacgcagcg gctgccgttc tacggcagcg cggtcgtgtg catcgacgac gcgaacgtgc ggcagatcgt gccgctgatc tcgaagcccg tcgtgcgcta cggcttcgcg gcggatgcgc aggtgcgcgc ggagaacgtc gaggcgcgcg acggccggat gcacttcacg gtgcggcgcg aggggcgcga gccgctgcct gtcgtcctga acctgcccgg cctgcacaac gtgcagaacg cgctcgcggc gatcgcgatc gcgaccgatc tcgatgtggc cgacgcggcg atccagcagg cgctcgcgga attcaacggc gtcggccggc gcttccagcg ctacggcgag atcgcggcgg cgggcggcgg cgcgtatacg ctgatcgacg actacggcca ccatccggtc gagatggcgg cgacgatcgc ggcggcgcgc ggcgcgttcc cgggccgccg gctcgtgctc gcgttccagc cgcatcgcta cacgcgcacg cgcgactgct tcgacgactt cgtcaacgtg ctgtcgacgg tcgacgcgct cgtgctgacc gaggtgtacg cggcgggcga ggcgccgatt tcgaccgcga acggcgacgc gctgtcgcgc gcgctgcgcg cggcgggcaa ggtcgagccg gtattcgtcg cgacggtcga cgaggtgccg gacgcgctcg cgaagctcgc gcgcgacggc gacgtggtga tcacgatggg cgcgggctcg atcggcggcg tgccgggcaa gctcgcgcag gacacgcaac agaagggatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BupsA.00137.b.B1.PW25574

PURIFICATION DATe:	1/19/2010
CONCENTRATION:	25.74mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	17
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

MKHIVKHIHF VGIGGAGMSG IAEVLVNLGY QVSGSDLARN AVTERLEALG ARVSIGHDAA NIEGANAVVV STAVRSDNPE VLAARRLRVP IVPRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLTSAGANAR LGTGDFIVAE ADESDASFLN LYPVIEVITN IDADHMDTYG HDFARLKQAF IEFTQRLPFY GSAVVCIDDA NVRQIVPLIS KPVVRYGFAA DAQVRAENVE ARDGRMXFTV RREGREPLPV VLNLPGLHNV QNALAAIAIA TDLDVXXAAI QQALAEFNGV GRRFXRXGXI AAAGGGAYXL IDDYGHHPVE MAATIAAARG AXPGRRLVLA XQPHRYTRTR DCFDDFVNVL STVDALVLTE VYAAGEAPIS TANGDALSRA LRAAGKVXPV FVATVDEVPD ALAKLARDGX XVITMGAGSI GGVPGKLAQ

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC ACATCGTCAA ACACATTCAC TTCGTCGGGA TCGGCGGCGC GGGCATGAGC GGCATCGCCG AAGTGCTCGT GAACCTGGGC TACCAGGTGA GCGGCTCCGA TCTCGCGCGC AACGCGGTGA CCGAGCGCCT CGAGGCGCTC GGCGCGCGCG TGTCGATCGG CCACGATGCG GCGAACATCG AAGGCGCGAA CGCGGTCGTC GTGTCGACCG CGGTGCGCTC GGACAACCCG GAAGTGCTCG CCGCGCGCCG CCTGCGCGTG CCGATCGTGC CGCGCGCGGT GATGCTCGCG GAGCTGATGC GCCTGAAGCA GGGGATCGCG ATCGCCGGCA CGCACGGCAA GACGACGACG ACGAGCCTCG TCGCGAGCGT GCTCGCCGCG GGCGGGCTCG ATCCGACGTT CGTGATCGGC GGGCGTCTCA CGAGCGCCGG CGCGAACGCG CGGCTCGGCA CGGGCGACTT CATCGTCGCG GAAGCCGACG AGTCGGACGC GTCATTCCTG AACCTGTATC CGGTGATCGA GGTCATCACG AACATCGACG CCGATCACAT GGATACCTAC GGCCACGACT TCGCGCGGCT CAAGCAGGCG TTCATCGAAT TCACGCAGCG GCTGCCGTTC TACGGCAGCG CGGTCGTGTG CATCGACGAC GCGAACGTGC GGCAGATCGT GCCGCTGATC TCGAAGCCCG TCGTGCGCTA CGGCTTCGCG GCGGATGCGC AGGTGCGCGC GGAGAACGTC GAGGCGCGCG ACGGCCGGAT GCACTTCACG GTGCGGCGCG AGGGGCGCGA GCCGCTGCCT GTCGTCCTGA ACCTGCCCGG CCTGCACAAC GTGCAGAACG CGCTCGCGGC GATCGCGATC GCGACCGATC TCGATGTGGC CGACGCGGCG ATCCAGCAGG CGCTCGCGGA ATTCAACGGC GTCGGCCGGC GCTTCCAGCG CTACGGCGAG ATCGCGGCGG CGGGCGGCGG CGCGTATACG CTGATCGACG ACTACGGCCA CCATCCGGTC GAGATGGCGG CGACGATCGC GGCGGCGCGC GGCGCGTTCC CGGGCCGCCG GCTCGTGCTC GCGTTCCAGC CGCATCGCTA CACGCGCACG CGCGACTGCT TCGACGACTT CGTCAACGTG CTGTCGACGG TCGACGCGCT CGTGCTGACC GAGGTGTACG CGGCGGGCGA GGCGCCGATT TCGACCGCGA ACGGCGACGC GCTGTCGCGC GCGCTGCGCG CGGCGGGCAA GGTCGAGCCG GTATTCGTCG CGACGGTCGA CGAGGTGCCG GACGCGCTCG CGAAGCTCGC GCGCGACGGC GACGTGGTGA TCACGATGGG CGCGGGCTCG ATCGGCGGCG TGCCGGGCAA GCTCGCGCAG GACACGCAAC AGAAGGGATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT