BupsA.00134.a

4-hydroxyphenylpyruvate dioxygenase

CENTER ID: BupsA.00134.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00134.a.A1.GE25243 Full length( BupsA.00134.a ) 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.12
RefSeq: YP_331434
UniProt: Q3JYB9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIPTWDNPV GTDGFEFIEY TAPDPKALGQ LFERMGFTAV ARHRHKDVTL YRQGDINFII NAEPDSFAQR FARLHGPSIC AIAFRVQDAA KAYRHALELG AWGFDNKTGP MELNIPAIKG IGDSLIYFVD RWRGKNGAKP GAIGDISIYD VDFEPIPGAD PNPAGHGLTY IDHLTHNVHR GRMQEWAEFY ERLFNFREVR YFDIEGKVTG VKSKAMTSPC GKIRIPINEE GSDTAGQIQE YLDAYRGEGI QHIALGAADI YRAVDGLRAK GVTLLDTIDT YYELVDRRVP NHGEPLDELR KRKILIDGAH DDLLLQIFTE NQIGPIFFEI IQRKGNQGFG EGNFKALFES IELDQIRRGV VQDKA
NT Sequence
ATGCAAATCC CCACCTGGGA CAATCCCGTC GGCACCGACG GCTTCGAATT CATCGAATAC ACCGCCCCCG ATCCGAAGGC GCTCGGCCAA CTGTTCGAGC GAATGGGCTT CACCGCGGTC GCCCGCCATC GCCACAAGGA CGTGACGCTG TACCGCCAGG GCGACATCAA CTTCATCATC AACGCGGAAC CCGATTCGTT CGCGCAACGC TTCGCGCGGC TGCACGGGCC GTCGATCTGC GCGATCGCAT TCCGCGTGCA GGACGCCGCG AAAGCGTACA GGCATGCGCT CGAGCTCGGC GCATGGGGCT TCGACAACAA GACGGGCCCG ATGGAGCTGA ACATCCCGGC GATCAAGGGC ATCGGCGATT CGCTGATCTA CTTCGTCGAC CGCTGGCGCG GCAAGAACGG CGCGAAGCCG GGCGCGATCG GCGATATCAG CATCTACGAC GTCGATTTCG AGCCGATTCC GGGCGCCGAT CCGAACCCGG CCGGCCACGG CCTCACGTAC ATCGATCACC TCACGCACAA CGTGCACCGC GGCCGCATGC AGGAATGGGC GGAGTTCTAC GAGCGCCTGT TCAACTTCCG CGAGGTTCGC TACTTCGACA TCGAAGGCAA GGTGACGGGC GTGAAATCGA AGGCGATGAC GTCGCCGTGC GGCAAGATCC GGATTCCGAT CAACGAGGAA GGCTCGGACA CGGCCGGCCA GATCCAGGAA TATCTGGACG CGTATCGCGG CGAAGGCATC CAGCACATCG CGCTCGGCGC GGCCGACATC TATCGGGCGG TCGACGGCCT GCGCGCGAAG GGCGTGACGC TGCTCGACAC GATCGACACG TACTACGAGC TCGTCGATCG CCGCGTGCCG AACCACGGCG AGCCGCTCGA CGAGCTCAGA AAGCGCAAGA TCCTGATCGA CGGCGCGCAC GACGATCTGC TGCTGCAGAT CTTCACCGAG AACCAGATCG GGCCGATCTT CTTCGAGATT ATTCAGCGCA AGGGTAATCA GGGTTTCGGC GAGGGCAACT TCAAGGCGCT GTTCGAATCG ATCGAACTCG ACCAGATCCG CCGCGGCGTC GTGCAGGACA AGGCG
Details for BupsA.00134.a.A1.GE25243
HARVESTED ON: 5/6/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQIPTWDNP VGTDGFEFIE YTAPDPKALG QLFERMGFTA VARHRHKDVT LYRQGDINFI INAEPDSFAQ RFARLHGPSI CAIAFRVQDA AKAYRHALEL GAWGFDNKTG PMELNIPAIK GIGDSLIYFV DRWRGKNGAK PGAIGDISIY DVDFEPIPGA DPNPAGHGLT YIDHLTHNVH RGRMQEWAEF YERLFNFREV RYFDIEGKVT GVKSKAMTSP CGKIRIPINE EGSDTAGQIQ EYLDAYRGER XPAHRARRGR XLSGGRRPAR EGRDAARHDR HVLRARRSPR XEPRRXXRRA XKAXXPDRRX A
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaaa tccccacctg ggacaatccc gtcggcaccg acggcttcga attcatcgaa tacaccgccc ccgatccgaa ggcgctcggc caactgttcg agcgaatggg cttcaccgcg gtcgcccgcc atcgccacaa ggacgtgacg ctgtaccgcc agggcgacat caacttcatc atcaacgcgg aacccgattc gttcgcgcaa cgcttcgcgc ggctgcacgg gccgtcgatc tgcgcgatcg cattccgcgt gcaggacgcc gcgaaagcgt acaggcatgc gctcgagctc ggcgcatggg gcttcgacaa caagacgggc ccgatggagc tgaacatccc ggcgatcaag ggcatcggcg attcgctgat ctacttcgtc gaccgctggc gcggcaagaa cggcgcgaag ccgggcgcga tcggcgatat cagcatctac gacgtcgatt tcgagccgat tccgggcgcc gatccgaacc cggccggcca cggcctcacg tacatcgatc acctcacgca caacgtgcac cgcggccgca tgcaggaatg ggcggagttc tacgagcgcc tgttcaactt ccgcgaggtt cgctacttcg acatcgaagg caaggtgacg ggcgtgaaat cgaaggcgat gacgtcgccg tgcggcaaga tccggattcc gatcaacgag gaaggctcgg acacggccgg ccagatccag gaatatctgg acgcgtatcg cggcgaaagg natccagcac atcgcgctcg gcgcggccga natctatcgg gcggtcgacg gcctgcgcgc gaagggcgtg acgctgctcg acacgatcga cacgtactac gagctcgtcg atcgccgcgt gncgaaccac ggcgannngn tcgacgagct canaaagcgc angancctga tcgacggnnc gcacga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQIPTWDNP VGTDGFEFIE YTAPDPKALG QLFERMGFTA VARHRHKDVT LYRQGDINFI INAEPDSFAQ RFARLHGPSI CAIAFRVQDA AKAYRHALEL GAWGFDNKTG PMELNIPAIK GIGDSLIYFV DRWRGKNGAK PGAIGDISIY DVDFEPIPGA DPNPAGHGLT YIDHLTHNVH RGRMQEWAEF YERLFNFREV RYFDIEGKVT GVKSKAMTSP CGKIRIPINE EGSDTAGQIQ EYLDAYRGEG IQHIALGAAD IYRAVDGLRA KGVTLLDTID TYYELVDRRV PNHGEPLDEL RKRKILIDGA HDDLLLQIFT ENQIGPIFFE IIQRKGNQGF GEGNFKALFE SIELDQIRRG VVQDKA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaaatcccc acctgggaca atcccgtcgg caccgacggc ttcgaattca tcgaatacac cgcccccgat ccgaaggcgc tcggccaact gttcgagcga atgggcttca ccgcggtcgc ccgccatcgc cacaaggacg tgacgctgta ccgccagggc gacatcaact tcatcatcaa cgcggaaccc gattcgttcg cgcaacgctt cgcgcggctg cacgggccgt cgatctgcgc gatcgcattc cgcgtgcagg acgccgcgaa agcgtacagg catgcgctcg agctcggcgc atggggcttc gacaacaaga cgggcccgat ggagctgaac atcccggcga tcaagggcat cggcgattcg ctgatctact tcgtcgaccg ctggcgcggc aagaacggcg cgaagccggg cgcgatcggc gatatcagca tctacgacgt cgatttcgag ccgattccgg gcgccgatcc gaacccggcc ggccacggcc tcacgtacat cgatcacctc acgcacaacg tgcaccgcgg ccgcatgcag gaatgggcgg agttctacga gcgcctgttc aacttccgcg aggttcgcta cttcgacatc gaaggcaagg tgacgggcgt gaaatcgaag gcgatgacgt cgccgtgcgg caagatccgg attccgatca acgaggaagg ctcggacacg gccggccaga tccaggaata tctggacgcg tatcgcggcg aaggcatcca gcacatcgcg ctcggcgcgg ccgacatcta tcgggcggtc gacggcctgc gcgcgaaggg cgtgacgctg ctcgacacga tcgacacgta ctacgagctc gtcgatcgcc gcgtgccgaa ccacggcgag ccgctcgacg agctcagaaa gcgcaagatc ctgatcgacg gcgcgcacga cgatctgctg ctgcagatct tcaccgagaa ccagatcggg ccgatcttct tcgagattat tcagcgcaag ggtaatcagg gtttcggcga gggcaacttc aaggcgctgt tcgaatcgat cgaactcgac cagatccgcc gcggcgtcgt gcaggacaag gcgtaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa