BupsA.00133.a

cysteinyl-tRNA synthetase

CENTER ID: BupsA.00133.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00133.a.A1.PS00454b Full length( BupsA.00133.a ) 1 465

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2806
RefSeq: YP_334067
UniProt: Q3JQT6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESLRIYNTL ARDKQDFVPR QPGEVRMYVC GITVYDYCHI GHARMVVVFD IVQRWLRARG YRVTYVRNIT DIDDKIIRRA VENGETIQSL TRRFTDAMNA DFDALGVERP DLEPRATEFI PQMLGMIEKL EANGYAYQAK DGDVNYSVRK FANYGRLSGK SLEDLRAGER VAANDAKEDP LDFVLWKRAK PQEPAGASWE SKYGAGRPGW HIECSAMGCT LLGAHFDIHG GGQDLQFPHH ENEIAQSEGA TGQTFVNYWM HNGFVQVDSE KMSKSLGNFF TIREVLEKFD AEVVRFFIVR THYRSPLNYS DVHLDDARAS LTRLYTALKD ATPDAQPLDW SEAHAQRFAA AMNDDFNTAV AVAVLFELAT EVNRTREPAL ARQLRLLAGL LGLLGREPRE FLQHAAGAAR TGALEPHEIE ARIAARVAAK QAKNYAEADR IRAELLEAGI ALEDKPGGST EWRRV
NT Sequence
ATGGAATCAC TGCGCATCTA CAACACGCTC GCGCGTGACA AGCAAGACTT CGTGCCGCGG CAGCCCGGCG AAGTGCGGAT GTACGTCTGC GGGATCACCG TCTACGACTA TTGCCACATC GGCCATGCGC GGATGGTGGT CGTGTTCGAC ATCGTCCAGC GCTGGTTGCG CGCGCGCGGC TATCGCGTGA CATACGTGCG CAACATCACC GACATCGACG ACAAGATCAT CCGCCGCGCG GTCGAGAACG GCGAGACGAT CCAGTCGCTC ACGCGCCGCT TCACCGACGC GATGAACGCG GATTTCGACG CGCTCGGCGT CGAGCGGCCG GACCTCGAGC CGCGCGCGAC CGAGTTCATC CCGCAGATGC TCGGGATGAT CGAGAAGCTC GAGGCGAACG GTTACGCGTA TCAGGCGAAG GACGGCGACG TCAACTATTC GGTGCGCAAG TTCGCGAACT ACGGCAGGCT CTCCGGCAAG TCGCTCGAGG ATTTGCGCGC GGGCGAGCGC GTCGCCGCGA ACGATGCGAA GGAGGATCCG CTCGACTTCG TGCTGTGGAA GCGCGCGAAG CCGCAGGAGC CGGCGGGCGC GTCGTGGGAA TCGAAGTACG GCGCGGGCCG CCCGGGCTGG CACATCGAAT GTTCGGCGAT GGGCTGCACG CTGCTCGGCG CGCACTTCGA CATTCACGGC GGCGGCCAGG ACCTGCAGTT CCCGCATCAC GAGAACGAGA TCGCGCAAAG CGAGGGTGCG ACCGGACAAA CTTTCGTCAA TTATTGGATG CACAACGGTT TCGTGCAGGT CGATAGTGAG AAGATGTCGA AGTCGCTCGG CAACTTCTTC ACGATCCGCG AAGTGCTCGA GAAGTTCGAC GCCGAGGTCG TGCGCTTCTT CATCGTTCGC ACGCATTACC GTTCGCCGCT CAACTACAGC GACGTTCATC TCGACGACGC GCGCGCATCG CTCACGCGCC TTTACACGGC ATTGAAGGAC GCGACGCCGG ATGCGCAGCC GCTCGACTGG AGCGAGGCGC ACGCGCAGCG TTTCGCCGCC GCGATGAACG ACGATTTCAA CACGGCGGTC GCCGTCGCCG TGCTGTTCGA GCTCGCGACC GAGGTGAACC GCACACGCGA GCCGGCGCTC GCGCGCCAGC TCAGGCTGCT TGCGGGCCTG CTCGGCCTGC TCGGCCGCGA GCCGCGCGAA TTCCTGCAGC ACGCGGCGGG CGCCGCGCGC ACGGGCGCGC TCGAGCCGCA TGAGATCGAA GCGAGGATCG CCGCGCGCGT CGCGGCGAAA CAGGCGAAGA ACTATGCCGA AGCGGACCGG ATCCGCGCGG AATTGCTCGA AGCCGGAATC GCACTTGAAG ACAAACCGGG CGGATCGACG GAGTGGCGCC GCGTA
Details for BupsA.00133.a.A1.PS00454b
PURIFICATION DATe: 1/5/2010
CONCENTRATION: 12.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 66
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMESLRIYNT LARDKQDFVP RQPGEVRMYV CGITVYDYCH IGHARMVVVF DIVQRWLRAR GYRVTYVRNI TDIDDKIIRR AVENGETIQS LTRRFTDAMN ADFDALGVER PDLEPRATEF IPQMLGMIEK LEANGYAYQA KDGDVNYSVR KFANYGRLSG KSLEDLRAGE RVAANDAKED PLDFVLWKRA KPQEPAGASW ESKYGAGRPG WHIECSAMGC TLLGAHFDIH GGGQDLQFPH HENEIAQSEG ATGQTFVNYW MHNGFVQVDS EKXSKSLGNF FXIREVXEKF DAXXXXFFXX RTXXR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaat cactgcgcat ctacaacacg ctcgcgcgtg acaagcaaga cttcgtgccg cggcagcccg gcgaagtgcg gatgtacgtc tgcgggatca ccgtctacga ctattgccac atcggccatg cgcggatggt ggtcgtgttc gacatcgtcc agcgctggtt gcgcgcgcgc ggctatcgcg tgacatacgt gcgcaacatc accgacatcg acgacaagat catccgccgc gcggtcgaga acggcgagac gatccagtcg ctcacgcgcc gcttcaccga cgcgatgaac gcggatttcg acgcgctcgg cgtcgagcgg ccggacctcg agccgcgcgc gaccgagttc atcccgcaga tgctcgggat gatcgagaag ctcgaggcga acggttacgc gtatcaggcg aaggacggcg acgtcaacta ttcggtgcgc aagttcgcga actacggcag gctctccggc aagtcgctcg aggatttgcg cgcgggcgag cgcgtcgccg cgaacgatgc gaaggaggat ccgctcgact tcgtgctgtg gaagcgcgcg aagccgcagg agccggcggg cgcgtcgtgg gaatcgaagt acggcgcggg ccgcccgggc tggcacatcg aatgttcggc gatgggctgc acgctgctcg gcgcgcactt cgacattcac ggcggcggcc aggacctgca gttcccgcat cacgagaacg agatcgcgca aagcgagggt gcgaccggac aaactttcgt caattattgg atgcacaacg gtttcgtgca ggtcgatagt gagaagangt cgaagtcnct cggcaacttc ttcncgatcc gcgaagtgnt cgagaagttc gacgccnang ncgngnnctt cttcntcgnn cgcacgcntt ancgntc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMESLRIYNT LARDKQDFVP RQPGEVRMYV CGITVYDYCH IGHARMVVVF DIVQRWLRAR GYRVTYVRNI TDIDDKIIRR AVENGETIQS LTRRFTDAMN ADFDALGVER PDLEPRATEF IPQMLGMIEK LEANGYAYQA KDGDVNYSVR KFANYGRLSG KSLEDLRAGE RVAANDAKED PLDFVLWKRA KPQEPAGASW ESKYGAGRPG WHIECSAMGC TLLGAHFDIH GGGQDLQFPH HENEIAQSEG ATGQTFVNYW MHNGFVQVDS EKMSKSLGNF FTIREVLEKF DAEVVRFFIV RTHYRSPLNY SDVHLDDARA SLTRLYTALK DATPDAQPLD WSEAHAQRFA AAMNDDFNTA VAVAVLFELA TEVNRTREPA LARQLRLLAG LLGLLGREPR EFLQHAAGAA RTGALEPHEI EARIAARVAA KQAKNYAEAD RIRAELLEAG IALEDKPGGS TEWRRV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATCACTG CGCATCTACA ACACGCTCGC GCGTGACAAG CAAGACTTCG TGCCGCGGCA GCCCGGCGAA GTGCGGATGT ACGTCTGCGG GATCACCGTC TACGACTATT GCCACATCGG CCATGCGCGG ATGGTGGTCG TGTTCGACAT CGTCCAGCGC TGGTTGCGCG CGCGCGGCTA TCGCGTGACA TACGTGCGCA ACATCACCGA CATCGACGAC AAGATCATCC GCCGCGCGGT CGAGAACGGC GAGACGATCC AGTCGCTCAC GCGCCGCTTC ACCGACGCGA TGAACGCGGA TTTCGACGCG CTCGGCGTCG AGCGGCCGGA CCTCGAGCCG CGCGCGACCG AGTTCATCCC GCAGATGCTC GGGATGATCG AGAAGCTCGA GGCGAACGGT TACGCGTATC AGGCGAAGGA CGGCGACGTC AACTATTCGG TGCGCAAGTT CGCGAACTAC GGCAGGCTCT CCGGCAAGTC GCTCGAGGAT TTGCGCGCGG GCGAGCGCGT CGCCGCGAAC GATGCGAAGG AGGATCCGCT CGACTTCGTG CTGTGGAAGC GCGCGAAGCC GCAGGAGCCG GCGGGCGCGT CGTGGGAATC GAAGTACGGC GCGGGCCGCC CGGGCTGGCA CATCGAATGT TCGGCGATGG GCTGCACGCT GCTCGGCGCG CACTTCGACA TTCACGGCGG CGGCCAGGAC CTGCAGTTCC CGCATCACGA GAACGAGATC GCGCAAAGCG AGGGTGCGAC CGGACAAACT TTCGTCAATT ATTGGATGCA CAACGGTTTC GTGCAGGTCG ATAGTGAGAA GATGTCGAAG TCGCTCGGCA ACTTCTTCAC GATCCGCGAA GTGCTCGAGA AGTTCGACGC CGAGGTCGTG CGCTTCTTCA TCGTTCGCAC GCATTACCGT TCGCCGCTCA ACTACAGCGA CGTTCATCTC GACGACGCGC GCGCATCGCT CACGCGCCTT TACACGGCAT TGAAGGACGC GACGCCGGAT GCGCAGCCGC TCGACTGGAG CGAGGCGCAC GCGCAGCGTT TCGCCGCCGC GATGAACGAC GATTTCAACA CGGCGGTCGC CGTCGCCGTG CTGTTCGAGC TCGCGACCGA GGTGAACCGC ACACGCGAGC CGGCGCTCGC GCGCCAGCTC AGGCTGCTTG CGGGCCTGCT CGGCCTGCTC GGCCGCGAGC CGCGCGAATT CCTGCAGCAC GCGGCGGGCG CCGCGCGCAC GGGCGCGCTC GAGCCGCATG AGATCGAAGC GAGGATCGCC GCGCGCGTCG CGGCGAAACA GGCGAAGAAC TATGCCGAAG CGGACCGGAT CCGCGCGGAA TTGCTCGAAG CCGGAATCGC ACTTGAAGAC AAACCGGGCG GATCGACGGA GTGGCGCCGC GTATGAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA