BupsA.00131.a

pyridoxal phosphate biosynthetic protein

CENTER ID:	BupsA.00131.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00131.a.A1.GE25241	Full length( BupsA.00131.a )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

3GK0

DEPOSITED: 3/9/2009

DETERMINATION: XRay

Diffraction Image

CLONE: BupsA.00131.a.A1.GE25241

PROTEIN: BupsA.00131.a.A1.PS00037

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.

Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC

PLoS ONE - 2012

volume 8, issue 1, pages e53851

PMID: 23382856; PMCID: PMC3561365

PDB: 3D63, 3DAH, 3EIZ, 3EJ2, 3EK2, 3EZO, 3F0F, 3FTP, 3GK0, 3GK3, 3GVF, 3GWA, 3GWE, 3IML, 3SZ8, 3T4C, 3TML, 3TMQ, 3TXY, 3U7J, 3UE9, 3UK1, 3UK2, 3UND, 3UPT, 3URR, 3UW1, 3UW2, 3UW3, 3V2I, 3V7N, 3V8H, 3V9O, 3V9P, 3VAV, 4DDO, 4DFE, 4DHE, 4DHK, 4DUT, 4DZ4, 4E4T, 4E51, 4EFI, 4EG0, 4EGJ, 4EK2, 4EQY, 4EWG, 4EX5, 4EXQ, 4F2G, 4F32, 4F3N, 4F3Y, 4F4H, 4F7D, 4FK8, 4FRY, 4G1K, 4G67, 4G6Z, 4GHK, 4H3Y, 4H3Z, 4H4G

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_136924
PATRIC ID:	fig\|320372.6.peg.3021
RefSeq:	YP_334273
UniProt:	Q3JQ80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFFLTTPAA IDLGVNIDHV ATLRNARGTA YPDPVRAALA AEDAGADAIT LHLREDRRHI VDADVRTLRP RVKTRMNLEC AVTPEMLDIA CEIRPHDACL VPEKRSELTT EGGLDVVGHF DAVRAACKQL ADAGVRVSLF IDPDEAQIRA AHETGAPVIE LHTGRYADAH DAAEQQREFE RIATGVDAGI ALGLKVNAGH GLHYTNVQAI AALPGIAELN IGHAIVAHAV FVGWDNAVRE MKAIMVAARV AALHGGR

NT Sequence

ATGAGCTTCT TCCTGACAAC GCCCGCCGCC ATCGACCTGG GCGTGAACAT CGACCACGTC GCGACGCTGC GCAATGCGCG CGGCACCGCG TATCCGGACC CCGTGCGCGC GGCGCTCGCC GCCGAGGACG CGGGCGCCGA CGCGATCACG CTGCACCTGC GCGAAGACCG CCGGCACATC GTCGACGCGG ACGTGCGCAC GCTGCGTCCG CGCGTGAAGA CGCGGATGAA TCTCGAGTGC GCGGTGACGC CGGAGATGCT CGACATCGCA TGCGAGATCC GGCCGCACGA CGCGTGCCTC GTGCCGGAGA AGCGTTCGGA GCTGACGACG GAGGGCGGTC TGGACGTCGT CGGGCACTTC GACGCGGTGC GCGCCGCATG CAAGCAGCTC GCGGATGCGG GCGTGCGCGT GTCGCTCTTC ATCGATCCGG ACGAGGCGCA GATCCGCGCC GCGCACGAGA CGGGCGCGCC CGTGATCGAA CTGCACACCG GCCGCTACGC GGACGCGCAC GACGCGGCCG AGCAGCAGCG CGAGTTCGAG CGGATCGCGA CGGGCGTCGA CGCCGGCATT GCGCTCGGGC TGAAGGTGAA CGCGGGCCAC GGATTGCACT ACACGAACGT GCAGGCGATC GCCGCGCTGC CCGGCATCGC CGAGCTGAAC ATCGGGCACG CGATCGTCGC GCACGCGGTG TTCGTCGGCT GGGACAACGC GGTGCGCGAG ATGAAGGCGA TCATGGTCGC CGCGCGCGTC GCCGCGCTGC ACGGCGGCCG C

Details for BupsA.00131.a.A1.GE25241

HARVESTED ON:	5/6/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSFFLTTPA AIDLGVNIDH VATLRNARGT AYPDPVRAAL AAEDAGADAI TLHLREDRRH IVDADVRTLR PRVKTRMNLE CAVTPEMLDI ACEIRPHDAC LVPEKRSELT TEGGLDVVGH FDAVRAACKQ LADAGVRVSL FIDPDEAQIR AAHETGAPVI ELHTGRYADA HDAAEQQREF ERIATGVDAG IALGLKVNAG HGLHYTNVQA IAALPGIAXL NIGHAIVAHA VFVGWDNAVR EMKAIMVAAR VAALHGGR

Validated NT Sequence

tttaagaagg agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagctt cttcctgaca acgcccgccg ccatcgacct gggcgtgaac atcgaccacg tcgcgacgct gcgcaatgcg cgcggcaccg cgtatccgga ccccgtgcgc gcggcgctcg ccgccgagga cgcgggcgcc gacgcgatca cgctgcacct gcgcgaagac cgccggcaca tcgtcgacgc ggacgtgcgc acgctgcgtc cgcgcgtgaa gacgcggatg aatctcgagt gcgcggtgac gccggagatg ctcgacatcg catgcgagat ccggccgcac gacgcgtgcc tcgtgccgga gaagcgttcg gagctgacga cggagggcgg tctggacgtc gtcgggcact tcgacgcggt gcgcgccgca tgcaagcagc tcgcggatgc gggcgtgcgc gtgtcgctct tcatcgatcc ggacgaggcg cagatccgcg ccgcgcacga gacgggcgcg cccgtgatcg aactgcacac cggccgctac gcggacgcgc acgacgcggc cgagcagcag cgcgagttcg agcggatcgc gacgggcgtc gacgccggca ttgcgctcgg gctgaaggtg aacgcgggcc acggattgca ctacacgaac gtgcaggcga tcgccgcgct gcccggcatc gccganctga acatcgggca cgcgatcgtc gcgcacgcgg tgttcgtcgg ctgggacaac gcggtgcgcg agatgaaggc gatcatggtc gccgcgcgcg tcgccgcgct gcacggcggc cgctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcttcttc ctgacaacgc ccgccgccat cgacctgggc gtgaacatcg accacgtcgc gacgctgcgc aatgcgcgcg gcaccgcgta tccggacccc gtgcgcgcgg cgctcgccgc cgaggacgcg ggcgccgacg cgatcacgct gcacctgcgc gaagaccgcc ggcacatcgt cgacgcggac gtgcgcacgc tgcgtccgcg cgtgaagacg cggatgaatc tcgagtgcgc ggtgacgccg gagatgctcg acatcgcatg cgagatccgg ccgcacgacg cgtgcctcgt gccggagaag cgttcggagc tgacgacgga gggcggtctg gacgtcgtcg ggcacttcga cgcggtgcgc gccgcatgca agcagctcgc ggatgcgggc gtgcgcgtgt cgctcttcat cgatccggac gaggcgcaga tccgcgccgc gcacgagacg ggcgcgcccg tgatcgaact gcacaccggc cgctacgcgg acgcgcacga cgcggccgag cagcagcgcg agttcgagcg gatcgcgacg ggcgtcgacg ccggcattgc gctcgggctg aaggtgaacg cgggccacgg attgcactac acgaacgtgc aggcgatcgc cgcgctgccc ggcatcgccg agctgaacat cgggcacgcg atcgtcgcgc acgcggtgtt cgtcggctgg gacaacgcgg tgcgcgagat gaaggcgatc atggtcgccg cgcgcgtcgc cgcgctgcac ggcggccgct gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa