SSGCID: Target Page

BupsA.00124.a

imidazoleglycerol-phosphate dehydratase

CENTER ID:	BupsA.00124.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00124.a.A1.GE25235	Full length( BupsA.00124.a )		1	195

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3842
RefSeq:	YP_335055
UniProt:	Q3JMZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVAQVVRNT SETQISVKID LDGTGRQKLA TGVPFLDHML DQIARHGLVD LDIEANGDTH IDDHHTVEDV GITLGQAVAK AVGDRKGIRR YGHSYVPLDE ALSRVVIDFS GRPGLEFHVP FTRARIGTFD VDLSIEFFRG FVNHAGVTLH IDNLRGVNAH HQLETVFKAF GRALRMAVEL DERAAGQIPS TKGSL

NT Sequence

ATGCGCGTGG CGCAAGTCGT TCGCAACACC AGCGAAACGC AGATCAGTGT GAAGATCGAC CTCGACGGCA CCGGCCGGCA GAAGCTTGCG ACGGGCGTCC CGTTTCTCGA CCATATGCTC GACCAGATCG CGCGCCATGG TCTCGTCGAT CTCGACATCG AAGCGAATGG GGACACGCAC ATCGACGATC ACCATACGGT CGAGGACGTC GGCATCACGC TCGGCCAGGC CGTCGCGAAA GCGGTCGGCG ACAGGAAGGG CATCCGCCGC TACGGCCATT CGTACGTGCC GCTCGACGAG GCGCTGTCGC GCGTCGTGAT CGACTTCTCC GGCCGTCCGG GCCTCGAATT CCATGTGCCG TTCACGCGCG CGCGGATCGG CACGTTCGAC GTCGACCTGT CGATCGAGTT CTTCCGCGGC TTCGTCAATC ACGCGGGCGT CACGCTGCAT ATCGACAATC TGCGCGGCGT CAATGCGCAC CATCAGCTCG AGACGGTGTT CAAGGCGTTC GGCCGCGCGC TGCGGATGGC GGTGGAACTC GACGAGCGCG CGGCGGGGCA GATTCCGTCG ACGAAGGGCA GCCTC

Details for BupsA.00124.a.A1.GE25235

HARVESTED ON:	5/6/2008
SEQUENCED ON:	7/29/2008
EXPECTED MW:	23kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRVAQVVRN TSETQISVKI DLDGTGRQKL ATGVPFLDHM LDQIARHGLV DLDIEANGDT HIDDHHTVED VGITLGQAVA KAVGDRKGIR RYGHSYVPLD EALSRVVIDF SGRPGLEFHV PFTRARIGTF DVDLSIEFFR GFVNHAGVTL HIDNLRGVNA HHQLETVFKA FGRALRMAVE LDERAAGQIP STKGSL

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcg tggcgcaagt cgttcgcaac accagcgaaa cgcagatcag tgtgaagatc gacctcgacg gcaccggccg gcagaagctt gcgacgggcg tcccgtttct cgaccatatg ctcgaccaga tcgcgcgcca tggtctcgtc gatctcgaca tcgaagcgaa tggggacacg cacatcgacg atcaccatac ggtcgaggac gtcggcatca cgctcggcca ggccgtcgcg aaagcggtcg gcgacaggaa gggcatccgc cgctacggcc attcgtacgt gccgctcgac gaggcgctgt cgcgcgtcgt gatcgacttc tccggccgtc cgggcctcga attccatgtg ccgttcacgc gcgcgcggat cggcacgttc gacgtcgacc tgtcgatcga gttcttccgc ggcttcgtca atcacgcggg cgtcacgctg catatcgaca atctgcgcgg cgtcaatgcg caccatcagc tcgagacggt gttcaaggcg ttcggccgcg cgctgcggat ggcggtggaa ctcgacgagc gcgcggcggg gcagattccg tcgacgaagg gcagcctcta ataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgtggcg caagtcgttc gcaacaccag cgaaacgcag atcagtgtga agatcgacct cgacggcacc ggccggcaga agcttgcgac gggcgtcccg tttctcgacc atatgctcga ccagatcgcg cgccatggtc tcgtcgatct cgacatcgaa gcgaatgggg acacgcacat cgacgatcac catacggtcg aggacgtcgg catcacgctc ggccaggccg tcgcgaaagc ggtcggcgac aggaagggca tccgccgcta cggccattcg tacgtgccgc tcgacgaggc gctgtcgcgc gtcgtgatcg acttctccgg ccgtccgggc ctcgaattcc atgtgccgtt cacgcgcgcg cggatcggca cgttcgacgt cgacctgtcg atcgagttct tccgcggctt cgtcaatcac gcgggcgtca cgctgcatat cgacaatctg cgcggcgtca atgcgcacca tcagctcgag acggtgttca aggcgttcgg ccgcgcgctg cggatggcgg tggaactcga cgagcgcgcg gcggggcaga ttccgtcgac gaagggcagc ctctgaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa