BupsA.00122.a
2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF)
| CENTER ID: | BupsA.00122.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00122.a.A1.PW33584 | Full length( BupsA.00122.a ) | 1 | 162 | unavailable | |
| BupsA.00122.a.A1.PW33584a | Full length( BupsA.00122.a ) | 1 | 162 | unavailable |
Structures
3F0D
DEPOSITED: 10/24/2008
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3F0E
DEPOSITED: 10/24/2008
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3F0F
DEPOSITED: 10/24/2008
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3F0G
DEPOSITED: 10/24/2008
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3IEQ
DEPOSITED: 7/23/2009
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3IEW
DEPOSITED: 7/23/2009
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3IKE
DEPOSITED: 8/5/2009
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3IKF
DEPOSITED: 8/5/2009
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3JVH
DEPOSITED: 9/16/2009
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3K14
DEPOSITED: 9/25/2009
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3K2X
DEPOSITED: 9/30/2009
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3KE1
DEPOSITED: 10/23/2009
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3MBM
DEPOSITED: 3/25/2010
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PS00036
3P0Z
DEPOSITED: 9/29/2010
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW29971
3P10
DEPOSITED: 9/29/2010
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW29971
3Q8H
DEPOSITED: 1/6/2011
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW29971
3QHD
DEPOSITED: 1/25/2011
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW29971
6MWF
DEPOSITED: 10/29/2018
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW28612
6MWI
DEPOSITED: 10/29/2018
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW28612
6MWJ
DEPOSITED: 10/29/2018
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW28612
6MWK
DEPOSITED: 10/29/2018
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW28612
6NMO
DEPOSITED: 1/11/2019
DETERMINATION: XRay
CLONE: BupsA.00122.a.D11.GE00453
PROTEIN: BupsA.00122.a.D11.PD00455
6V3M
DEPOSITED: 11/26/2019
DETERMINATION: XRay
CLONE: BupsA.00122.a.A1.GE25234
PROTEIN: BupsA.00122.a.A1.PW28612
Publications by SSGCID
Leveraging structure determination with fragment screening for infectious disease drug targets: MECP synthase from Burkholderia pseudomallei.
Abendroth J, Begley DW, Bhandari J, Burris CA, Davies DR, Edwards TE, Hartley RC, Leonard JT, Myler PJ, Staker BL, Stewart LJ
J. Struct. Funct. Genomics - 2011
volume 12, issue 2, pages 63-76
PMID: 21359640; PMCID: PMC3123455
Cytidine derivatives as IspF inhibitors of Burkolderia pseudomallei.
Begley DW, Blain J, Clare M, Edwards TE, Gogol K, Hagen TJ, Hartley RC, Horn JR, Jakkaraju S, Karlsson CA, Myler PJ, Staker BL, Stewart LJ, Zhang Z, Zhao L
Bioorg. Med. Chem. Lett. - 2013
volume 23, issue 24, pages 6860-3
PMID: 24157367; PMCID: PMC3874807
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130616 |
| PATRIC ID: | fig|320372.6.peg.2627 |
| RefSeq: | YP_333903 |
| UniProt: | Q3JRA0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFRIGQGYD VHQLVPGRPL IIGGVTIPYE RGLLGHSDAD VLLHAITDAL FGAAALGDIG RHFSDTDPRF KGADSRALLR ECASRVAQAG FAIRNVDSTI IAQAPKLAPH IDAMRANIAA DLDLPLDRVN VKAKTNEKLG YLGRGEGIEA QAAALVVREA AA
NT Sequence
ATGGATTTCA GAATCGGACA AGGCTACGAC GTGCACCAGC TCGTTCCCGG ACGCCCGCTC ATCATCGGCG GCGTGACGAT CCCTTACGAG CGGGGGCTGC TCGGCCACTC GGACGCCGAC GTGCTGCTGC ACGCGATCAC CGACGCGCTC TTCGGCGCGG CGGCGCTCGG CGACATCGGC CGCCACTTCT CCGACACCGA TCCGCGCTTC AAGGGCGCCG ACAGCCGCGC GCTGTTGCGC GAATGCGCAT CGCGCGTCGC GCAGGCGGGT TTTGCGATCC GCAACGTCGA CAGCACGATC ATCGCGCAGG CGCCGAAGCT CGCGCCGCAT ATCGACGCGA TGCGCGCGAA CATCGCGGCC GATCTCGACT TGCCGCTCGA TCGCGTGAAC GTGAAGGCGA AGACGAACGA GAAGCTCGGC TATCTCGGGC GCGGTGAGGG GATCGAGGCG CAGGCGGCCG CGCTCGTCGT GCGCGAAGCG GCCGCG
Details for BupsA.00122.a.A1.PW33584
| PURIFICATION DATe: | 7/5/2011 |
| CONCENTRATION: | 31.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDFRIGQGY DVHQLVPGRP LIIGGVTIPY ERGLLGHSDA DVLLHAITDA LFGAAALGDI GRHFSDTDPR FKGADSRALL RECASRVAQA GFAIRNVDST IIAQAPKLAP HIDAMRANIA ADLDLPLDRV NVKAKTNEKL GYLGRGEGIE AQAAALVVRE AAA
Validated NT Sequence
ttattacgcg gccgcttcgc gcacgacgag cgcggccgcc tgcgcctcga tcccctcacc gcgcccgaga tagccgagct tctcgttcgt cttcgccttc acgttcacgc gatcgagcgg caagtcgaga tcggccgcga tgttcgcgcg catcgcgtcg atatgcggcg cgagcttcgg cgcctgcgcg atgatcgtgc tgtcgacgtt gcggatcgca aaacccgcct gcgcgacgcg cgatgcgcat tcgcgcaaca gcgcgcggct gtcggcgccc ttgaagcgcg gatcggtgtc ggagaagtgg cggccgatgt cgccgagcgc cgccgcgccg aagagcgcgt cggtgatcgc gtgcagcagc acgtcggcgt ccgagtggcc gagcagcccc cgctcgtaag ggatcgtcac gccgccgatg atgagcgggc gtccgggaac gagctggtgc acgtcgtagc cttgtccgat tctgaaatcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFRIGQGY DVHQLVPGRP LIIGGVTIPY ERGLLGHSDA DVLLHAITDA LFGAAALGDI GRHFSDTDPR FKGADSRALL RECASRVAQA GFAIRNVDST IIAQAPKLAP HIDAMRANIA ADLDLPLDRV NVKAKTNEKL GYLGRGEGIE AQAAALVVRE AAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATTTCAGA ATCGGACAAG GCTACGACGT GCACCAGCTC GTTCCCGGAC GCCCGCTCAT CATCGGCGGC GTGACGATCC CTTACGAGCG GGGGCTGCTC GGCCACTCGG ACGCCGACGT GCTGCTGCAC GCGATCACCG ACGCGCTCTT CGGCGCGGCG GCGCTCGGCG ACATCGGCCG CCACTTCTCC GACACCGATC CGCGCTTCAA GGGCGCCGAC AGCCGCGCGC TGTTGCGCGA ATGCGCATCG CGCGTCGCGC AGGCGGGTTT TGCGATCCGC AACGTCGACA GCACGATCAT CGCGCAGGCG CCGAAGCTCG CGCCGCATAT CGACGCGATG CGCGCGAACA TCGCGGCCGA TCTCGACTTG CCGCTCGATC GCGTGAACGT GAAGGCGAAG ACGAACGAGA AGCTCGGCTA TCTCGGGCGC GGTGAGGGGA TCGAGGCGCA GGCGGCCGCG CTCGTCGTGC GCGAAGCGGC CGCGTGAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for BupsA.00122.a.A1.PW33584a
| PURIFICATION DATe: | 7/5/2011 |
| CONCENTRATION: | 31.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDFRIGQGY DVHQLVPGRP LIIGGVTIPY ERGLLGHSDA DVLLHAITDA LFGAAALGDI GRHFSDTDPR FKGADSRALL RECASRVAQA GFAIRNVDST IIAQAPKLAP HIDAMRANIA ADLDLPLDRV NVKAKTNEKL GYLGRGEGIE AQAAALVVRE AAA
Validated NT Sequence
ttattacgcg gccgcttcgc gcacgacgag cgcggccgcc tgcgcctcga tcccctcacc gcgcccgaga tagccgagct tctcgttcgt cttcgccttc acgttcacgc gatcgagcgg caagtcgaga tcggccgcga tgttcgcgcg catcgcgtcg atatgcggcg cgagcttcgg cgcctgcgcg atgatcgtgc tgtcgacgtt gcggatcgca aaacccgcct gcgcgacgcg cgatgcgcat tcgcgcaaca gcgcgcggct gtcggcgccc ttgaagcgcg gatcggtgtc ggagaagtgg cggccgatgt cgccgagcgc cgccgcgccg aagagcgcgt cggtgatcgc gtgcagcagc acgtcggcgt ccgagtggcc gagcagcccc cgctcgtaag ggatcgtcac gccgccgatg atgagcgggc gtccgggaac gagctggtgc acgtcgtagc cttgtccgat tctgaaatcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFRIGQGY DVHQLVPGRP LIIGGVTIPY ERGLLGHSDA DVLLHAITDA LFGAAALGDI GRHFSDTDPR FKGADSRALL RECASRVAQA GFAIRNVDST IIAQAPKLAP HIDAMRANIA ADLDLPLDRV NVKAKTNEKL GYLGRGEGIE AQAAALVVRE AAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATTTCAGA ATCGGACAAG GCTACGACGT GCACCAGCTC GTTCCCGGAC GCCCGCTCAT CATCGGCGGC GTGACGATCC CTTACGAGCG GGGGCTGCTC GGCCACTCGG ACGCCGACGT GCTGCTGCAC GCGATCACCG ACGCGCTCTT CGGCGCGGCG GCGCTCGGCG ACATCGGCCG CCACTTCTCC GACACCGATC CGCGCTTCAA GGGCGCCGAC AGCCGCGCGC TGTTGCGCGA ATGCGCATCG CGCGTCGCGC AGGCGGGTTT TGCGATCCGC AACGTCGACA GCACGATCAT CGCGCAGGCG CCGAAGCTCG CGCCGCATAT CGACGCGATG CGCGCGAACA TCGCGGCCGA TCTCGACTTG CCGCTCGATC GCGTGAACGT GAAGGCGAAG ACGAACGAGA AGCTCGGCTA TCTCGGGCGC GGTGAGGGGA TCGAGGCGCA GGCGGCCGCG CTCGTCGTGC GCGAAGCGGC CGCGTGAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA