BupsA.00117.a

catechol 1,2-dioxygenase

CENTER ID: BupsA.00117.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00117.a.D12.GE00001 Full length( BupsA.00117.a ) 1 281
BupsA.00117.a.D13.GE00001 Full length( BupsA.00117.a ) 1 297
BupsA.00117.a.D15.GE00001 Full length( BupsA.00117.a ) 21 281
BupsA.00117.a.D16.GE00001 Full length( BupsA.00117.a ) 21 297
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00117.a.D13.PD00170 Full length( BupsA.00117.a ) 1 297
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_141371
PATRIC ID: fig|320372.6.peg.5043
RefSeq: YP_336145
UniProt: Q3JJV8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKEAIDALL KSFDDAATRP GNPRVRAIVN RIVKDLFYTI EDCDVQPSEF WTALNYLNDA GKEFGLIAAG LGFERFLDVR MDEAEAKAGL QGGTPRTIEG PLYVAGAPES VGHARLDDGT DPGETLVMRG RVLGGNGAPL ANALVEVWHA NHLGNYSYFD PSQPAFNLRR SIRTDAEGRY SFRSVLPVGY SVPPGSKTEQ LLDQLGRHGH RPAHIHFFVS APGHRKLTTQ INIEGDPHIW DDFAFATREG LIPKIAQAEG AQGKPYGIDG RFALIDFDFT LTRERGDVPA SEVERVRAQA
NT Sequence
ATGAACAAGG AAGCCATCGA CGCCCTGCTG AAATCGTTCG ACGACGCCGC GACGCGGCCC GGCAACCCGC GCGTGCGCGC GATCGTCAAC CGGATCGTGA AGGACCTGTT CTACACGATC GAGGATTGCG ACGTGCAGCC GAGCGAATTC TGGACCGCGC TCAACTACCT GAACGATGCC GGCAAGGAAT TCGGCCTCAT CGCGGCTGGC CTCGGCTTCG AGCGCTTCCT CGACGTGCGG ATGGACGAAG CCGAAGCGAA GGCGGGCCTG CAGGGCGGCA CGCCGCGCAC GATCGAGGGG CCGCTGTACG TCGCGGGCGC GCCGGAGTCG GTCGGCCACG CGCGGCTCGA CGACGGCACC GATCCGGGCG AGACGCTCGT GATGCGCGGC CGCGTGCTCG GCGGCAACGG CGCGCCGCTT GCGAACGCGC TCGTCGAAGT CTGGCATGCG AACCATCTCG GCAATTATTC GTACTTCGAT CCGTCTCAGC CCGCGTTCAA CCTGCGCCGC TCGATCCGCA CCGACGCCGA AGGCCGCTAC AGCTTCCGCA GCGTGCTGCC CGTCGGCTAC AGCGTGCCGC CCGGCAGCAA GACCGAGCAA TTGCTCGACC AGCTCGGCCG CCACGGCCAC CGCCCCGCGC ACATCCACTT CTTCGTGTCG GCGCCCGGCC ATCGCAAGCT GACGACGCAG ATCAACATCG AGGGCGATCC GCACATCTGG GACGACTTCG CGTTCGCGAC GCGCGAGGGG CTGATTCCGA AGATCGCGCA GGCGGAAGGC GCGCAGGGCA AGCCGTACGG CATCGACGGC CGGTTCGCGC TGATCGACTT CGATTTCACG CTGACGCGCG AGCGCGGCGA CGTGCCGGCG AGCGAAGTCG AGCGCGTGCG CGCGCAAGCG
Details for BupsA.00117.a.D12.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/29/2009
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 78
Validated AA Sequence
MEDNDXIEAX REQIGGMNKE AIDXLLKSFD DAATRPGNXR VRAIVNRIVK DLFYTIEDCD VQXSEFWTAX NYLNDAGKEF GLIAAGLGFE RFLDVRMDEA EAKAGLQGGT PRTIEGPLYV AGAPESVGHA RLDDGTDPGE TLVMRGRVLG GNGAPLANAL VEVWHANHLG NYSYFDPSQP AFNLRRSIRT DAEGRYSFRS VLPVGYSVPP GSKTEQLLDQ LGRHGHRPAH IHFFVSAPGH RKLTTQINIE GDPHIWDDFA FATREGLIPK IAQAEGAQGK PYGIDGRFAL IDFDFTL
Validated NT Sequence
ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagctttta cagagtgaag tcgaagtcga tcagggcgaa acgaccgtca ataccgtacg gtttgccctg agcaccctct gcctgtgcaa tcttcgggat caggccttca cgggtggcga atgcaaagtc gtcccagatg tgtgggtcac cttcgatgtt aatctgggtg gtcagcttgc ggtggcctgg cgcagaaaca aagaagtgaa tgtgggcagg gcggtggccg tgacggccta actgatctaa cagctgttca gtcttagagc ccggcggcac agagtagcct acagggagaa cagaacggaa gctgtaacgg ccttctgcgt cagtacgaat ggaacgacga aggttgaagg ccggctggga cgggtcgaag tagctatagt tgcctaaatg gttagcgtgc cagacttcaa cgagggcgtt tgccagcggt gcgccgttac cgccgagaac acggccacgc atcaccagag tttcaccagg atcagtgcca tcgtcgaggc gagcgtggcc aacagattcc ggagcgccag ctacgtacag agggccctca atagtacgcg gagtgccgcc ctggaggccc gccttagctt ctgcttcgtc catacgcaca tccaggaagc gttcgaagcc taagccggct gcgatcagac cgaattcttt acctgcatcg ttaaggtagt tcanggcagt ccagaattcg gaangctgca cgtcgcagtc ttcgatggtg tagaacagat ctttaacgat gcggttaact attgcacgaa cacganggtt gcccggacga gtagcagcgt catcgaagga cttgagcaga ncgtcaattg cttctttgtt cattccaccg atttgttcac ggngtgcttc gatnatgtcg ttatcttcca tgtccaganc ttncgnantc tggtctgncn gnanangnat ancntcnnac anaaannncn nnnnnnnatt tncnngcccn ggnntttann nannnnnncn ncngngacnn nnnngggnnn gnnnnnntnn nnannnnttc nnnnnnaccn nnnnnnnnnn nnnngnnnnn nnnnnnnnnn nnnnntnnnn gnnttnnnnn nncnnnnnnn nnnnnnnnnn ngg
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNKE AIDALLKSFD DAATRPGNPR VRAIVNRIVK DLFYTIEDCD VQPSEFWTAL NYLNDAGKEF GLIAAGLGFE RFLDVRMDEA EAKAGLQGGT PRTIEGPLYV AGAPESVGHA RLDDGTDPGE TLVMRGRVLG GNGAPLANAL VEVWHANHLG NYSYFDPSQP AFNLRRSIRT DAEGRYSFRS VLPVGYSVPP GSKTEQLLDQ LGRHGHRPAH IHFFVSAPGH RKLTTQINIE GDPHIWDDFA FATREGLIPK IAQAEGAQGK PYGIDGRFAL IDFDFTL
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaacaaagaa gcaattgacg ctctgctcaa gtccttcgat gacgctgcta ctcgtccggg caaccctcgt gttcgtgcaa tagttaaccg catcgttaaa gatctgttct acaccatcga agactgcgac gtgcagcctt ccgaattctg gactgccctg aactacctta acgatgcagg taaagaattc ggtctgatcg cagccggctt aggcttcgaa cgcttcctgg atgtgcgtat ggacgaagca gaagctaagg cgggcctcca gggcggcact ccgcgtacta ttgagggccc tctgtacgta gctggcgctc cggaatctgt tggccacgct cgcctcgacg atggcactga tcctggtgaa actctggtga tgcgtggccg tgttctcggc ggtaacggcg caccgctggc aaacgccctc gttgaagtct ggcacgctaa ccatttaggc aactatagct acttcgaccc gtcccagccg gccttcaacc ttcgtcgttc cattcgtact gacgcagaag gccgttacag cttccgttct gttctccctg taggctactc tgtgccgccg ggctctaaga ctgaacagct gttagatcag ttaggccgtc acggccaccg ccctgcccac attcacttct ttgtttctgc gccaggccac cgcaagctga ccacccagat taacatcgaa ggtgacccac acatctggga cgactttgca ttcgccaccc gtgaaggcct gatcccgaag attgcacagg cagagggtgc tcagggcaaa ccgtacggta ttgacggtcg tttcgccctg atcgacttcg acttcactct gtaaaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
Details for BupsA.00117.a.D13.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/30/2009
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNKE AIDALLKSFD DAATRPGNPR VRAIVNRIVK DLFYTIEDCD VQPSEFWTAL NYLNDAGKEF GLIAAGLGFE RFLDVRMDEA EAKAGLQGGT PRTIEGPLYV AGAPESVGHA RLDDGTDPGE TLVMRGRVLG GNGAPLANAL VEVWHANHLG NYSYFDPSQP AFNLRRSIRT DAEGRYSFRS VLPVGYSVPP GSKTEQLLDQ LGRHGHRPAH IHFFVSAPGH RKLTTQINIE GDPHIWDDFA FATREGLIPK IAQAEGAQGK PYGIDGRFAL IDFDFTLTRE RGDVPASEVE RVR
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaacaaagaa gcaattgacg ctctgctcaa gtccttcgat gacgctgcta ctcgtccggg caaccctcgt gttcgtgcaa tagttaaccg catcgttaaa gatctgttct acaccatcga agactgcgac gtgcagcctt ccgaattctg gactgccctg aactacctta acgatgcagg taaagaattc ggtctgatcg cagccggctt aggcttcgaa cgcttcctgg atgtgcgtat ggacgaagca gaagctaagg cgggcctcca gggcggcact ccgcgtacta ttgagggccc tctgtacgta gctggcgctc cggaatctgt tggccacgct cgcctcgacg atggcactga tcctggtgaa actctggtga tgcgtggccg tgttctcggc ggtaacggcg caccgctggc aaacgccctc gttgaagtct ggcacgctaa ccatttaggc aactatagct acttcgaccc gtcccagccg gccttcaacc ttcgtcgttc cattcgtact gacgcagaag gccgttacag cttccgttct gttctccctg taggctactc tgtgccgccg ggctctaaga ctgaacagct gttagatcag ttaggccgtc acggccaccg ccctgcccac attcacttct ttgtttctgc gccaggccac cgcaagctga ccacccagat taacatcgaa ggtgacccac acatctggga cgactttgca ttcgccaccc gtgaaggcct gatcccgaag attgcacagg cagagggtgc tcagggcaaa ccgtacggta ttgacggtcg tttcgccctg atcgacttcg acttcactct gacccgcgaa cgtggcgatg ttcctgctag cgaagtggaa cgtgttcgtt aa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNKE AIDALLKSFD DAATRPGNPR VRAIVNRIVK DLFYTIEDCD VQPSEFWTAL NYLNDAGKEF GLIAAGLGFE RFLDVRMDEA EAKAGLQGGT PRTIEGPLYV AGAPESVGHA RLDDGTDPGE TLVMRGRVLG GNGAPLANAL VEVWHANHLG NYSYFDPSQP AFNLRRSIRT DAEGRYSFRS VLPVGYSVPP GSKTEQLLDQ LGRHGHRPAH IHFFVSAPGH RKLTTQINIE GDPHIWDDFA FATREGLIPK IAQAEGAQGK PYGIDGRFAL IDFDFTLTRE RGDVPASEVE RVR
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaacaaagaa gcaattgacg ctctgctcaa gtccttcgat gacgctgcta ctcgtccggg caaccctcgt gttcgtgcaa tagttaaccg catcgttaaa gatctgttct acaccatcga agactgcgac gtgcagcctt ccgaattctg gactgccctg aactacctta acgatgcagg taaagaattc ggtctgatcg cagccggctt aggcttcgaa cgcttcctgg atgtgcgtat ggacgaagca gaagctaagg cgggcctcca gggcggcact ccgcgtacta ttgagggccc tctgtacgta gctggcgctc cggaatctgt tggccacgct cgcctcgacg atggcactga tcctggtgaa actctggtga tgcgtggccg tgttctcggc ggtaacggcg caccgctggc aaacgccctc gttgaagtct ggcacgctaa ccatttaggc aactatagct acttcgaccc gtcccagccg gccttcaacc ttcgtcgttc cattcgtact gacgcagaag gccgttacag cttccgttct gttctccctg taggctactc tgtgccgccg ggctctaaga ctgaacagct gttagatcag ttaggccgtc acggccaccg ccctgcccac attcacttct ttgtttctgc gccaggccac cgcaagctga ccacccagat taacatcgaa ggtgacccac acatctggga cgactttgca ttcgccaccc gtgaaggcct gatcccgaag attgcacagg cagagggtgc tcagggcaaa ccgtacggta ttgacggtcg tttcgccctg atcgacttcg acttcactct gacccgcgaa cgtggcgatg ttcctgctag cgaagtggaa cgtgttcgtt aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for BupsA.00117.a.D15.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/30/2009
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 91
PERCENT COVERAGE: 86
Validated AA Sequence
MXAFXXXXXX XXXXXFXYXX IRXQADQXXE DLXMXDNDIX EAXXEQIGXG NXRVRAIVNR IVKDLFYTIE XCDVQPSEFW TALNYLNDAG KEFGLIAAGL GFERFLDVRM DEAEAKAGXQ GGTPRTIEGP LYVAGAPESV GHARLDDGTD PGETLVMRGR VLGGNGAPLA NALVEVWHAN HLGNYSYFDP SQPAFNLRRS IRTDAEGRYS FRSVLPVGYS VPPGSKTEQL LDQLGRHGHR PAHIHFFVSA PGHRKLTTQI NIEGDPHIWD DFAFATREGL IPKIAQAEGA QGKPYGIDGR FALIDFDFTL
Validated NT Sequence
ttcnggcttt gttagcngcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagctttta cagagtgaag tcgaagtcga tcagggcgaa acgaccgtca ataccgtacg gtttgccctg agcaccctct gcctgtgcaa tcttcgggat caggccttca cgggtggcga atgcaaagtc gtcccagatg tgtgggtcac cttcgatgtt aatctgggtg gtcagcttgc ggtggcctgg cgcagaaaca aagaagtgaa tgtgggcagg gcggtggccg tgacggccta actgatctaa cagctgttca gtcttagagc ccggcggcac agagtagcct acagggagaa cagaacggaa gctgtaacgg ccttctgcgt cagtacgaat ggaacgacga aggttgaagg ccggctggga cgggtcgaag tagctatagt tgcctaaatg gttagcgtgc cagacttcaa cgagggcgtt tgccagcggt gcgccgttac cgccgagaac acggccacgc atcaccagag tttcaccagg atcagtgcca tcgtcgaggc gagcgtggcc aacagattcc ggagcgccag ctacgtacag agggccctca atagtacgcg gagtgccgcc ctggangccc gccttagctt ctgcttcgtc catacgcaca tccaggaagc gttcgaagcc taagccggct gcgatcagac cgaattcttt acctgcatcg ttaaggtagt tcagggcagt ccagaattcg gaaggctgca cgtcgcannc ttcgatggtg tagaacagat ctttaacgat gcggttaact attgcacgaa cacganggtt gcctcnaccg atttgttcac ngngngcttc nataatgtcg ttatcttnca tgnncagatc ttccngantc tggtctgcct gaanacggat acnntcgtac ngaaagnnnn ngnaanncan tnncnnnnnn nggtnannga atgctnncat cngncgacnn annngggnng nnnnnntnat naanaannnt nnnnnnnnnc nnngctnnnn tnnnnnnnnn gtggtnnnnn nnnnntnann nnnnnnntnn gngnnnnnnn nnnnngnnnn nnannnnnna atnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncnnt
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGGNPR VRAIVNRIVK DLFYTIEDCD VQPSEFWTAL NYLNDAGKEF GLIAAGLGFE RFLDVRMDEA EAKAGLQGGT PRTIEGPLYV AGAPESVGHA RLDDGTDPGE TLVMRGRVLG GNGAPLANAL VEVWHANHLG NYSYFDPSQP AFNLRRSIRT DAEGRYSFRS VLPVGYSVPP GSKTEQLLDQ LGRHGHRPAH IHFFVSAPGH RKLTTQINIE GDPHIWDDFA FATREGLIPK IAQAEGAQGK PYGIDGRFAL IDFDFTL
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggagg caaccctcgt gttcgtgcaa tagttaaccg catcgttaaa gatctgttct acaccatcga agactgcgac gtgcagcctt ccgaattctg gactgccctg aactacctta acgatgcagg taaagaattc ggtctgatcg cagccggctt aggcttcgaa cgcttcctgg atgtgcgtat ggacgaagca gaagctaagg cgggcctcca gggcggcact ccgcgtacta ttgagggccc tctgtacgta gctggcgctc cggaatctgt tggccacgct cgcctcgacg atggcactga tcctggtgaa actctggtga tgcgtggccg tgttctcggc ggtaacggcg caccgctggc aaacgccctc gttgaagtct ggcacgctaa ccatttaggc aactatagct acttcgaccc gtcccagccg gccttcaacc ttcgtcgttc cattcgtact gacgcagaag gccgttacag cttccgttct gttctccctg taggctactc tgtgccgccg ggctctaaga ctgaacagct gttagatcag ttaggccgtc acggccaccg ccctgcccac attcacttct ttgtttctgc gccaggccac cgcaagctga ccacccagat taacatcgaa ggtgacccac acatctggga cgactttgca ttcgccaccc gtgaaggcct gatcccgaag attgcacagg cagagggtgc tcagggcaaa ccgtacggta ttgacggtcg tttcgccctg atcgacttcg acttcactct gtaaaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
Details for BupsA.00117.a.D16.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/30/2009
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG GNPRVRAIVN RIVKDLFYTI EDCDVQPSEF WTALNYLNDA GKEFGLIAAG LGFERFLDVR MDEAEAKAGL QGGTPRTIEG PLYVAGAPES VGHARLDDGT DPGETLVMRG RVLGGNGAPL ANALVEVWHA NHLGNYSYFD PSQPAFNLRR SIRTDAEGRY SFRSVLPVGY SVPPGSKTEQ LLDQLGRHGH RPAHIHFFVS APGHRKLTTQ INIEGDPHIW DDFAFATREG LIPKIAQAEG AQGKPYGIDG RFALIDFDFT LTRERGDVPA SEVERVR
Validated NT Sequence
agcggcgaag taaaaccgga agtgaagccg gagacccaca tcaacctgaa ggttagcgac ggtagcagcg aaatcttctt caagattaag aagaccaccc cgctgcgtcg cctgatggaa gcattcgcta aacgccaggg caaggaaatg gattccctgc gctttctgta cgacggtatc cgtattcagg cagaccagac tccggaagat ctggacatgg aagataacga cattatcgaa gcacaccgtg aacaaatcgg tggaggcaac cctcgtgttc gtgcaatagt taaccgcatc gttaaagatc tgttctacac catcgaagac tgcgacgtgc agccttccga attctggact gccctgaact accttaacga tgcaggtaaa gaattcggtc tgatcgcagc cggcttaggc ttcgaacgct tcctggatgt gcgtatggac gaagcagaag ctaaggcggg cctccagggc ggcactccgc gtactattga gggccctctg tacgtagctg gcgctccgga atctgttggc cacgctcgcc tcgacgatgg cactgatcct ggtgaaactc tggtgatgcg tggccgtgtt ctcggcggta acggcgcacc gctggcaaac gccctcgttg aagtctggca cgctaaccat ttaggcaact atagctactt cgacccgtcc cagccggcct tcaaccttcg tcgttccatt cgtactgacg cagaaggccg ttacagcttc cgttctgttc tccctgtagg ctactctgtg ccgccgggct ctaagactga acagctgtta gatcagttag gccgtcacgg ccaccgccct gcccacattc acttctttgt ttctgcgcca ggccaccgca agctgaccac ccagattaac atcgaaggtg acccacacat ctgggacgac tttgcattcg ccacccgtga aggcctgatc ccgaagattg cacaggcaga gggtgctcag ggcaaaccgt acggtattga cggtcgtttc gccctgatcg acttcgactt cactctgacc cgcgaacgtg gcgatgttcc tgctagcgaa gtggaacgtg ttcgttaa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGGNPR VRAIVNRIVK DLFYTIEDCD VQPSEFWTAL NYLNDAGKEF GLIAAGLGFE RFLDVRMDEA EAKAGLQGGT PRTIEGPLYV AGAPESVGHA RLDDGTDPGE TLVMRGRVLG GNGAPLANAL VEVWHANHLG NYSYFDPSQP AFNLRRSIRT DAEGRYSFRS VLPVGYSVPP GSKTEQLLDQ LGRHGHRPAH IHFFVSAPGH RKLTTQINIE GDPHIWDDFA FATREGLIPK IAQAEGAQGK PYGIDGRFAL IDFDFTLTRE RGDVPASEVE RVR
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggagg caaccctcgt gttcgtgcaa tagttaaccg catcgttaaa gatctgttct acaccatcga agactgcgac gtgcagcctt ccgaattctg gactgccctg aactacctta acgatgcagg taaagaattc ggtctgatcg cagccggctt aggcttcgaa cgcttcctgg atgtgcgtat ggacgaagca gaagctaagg cgggcctcca gggcggcact ccgcgtacta ttgagggccc tctgtacgta gctggcgctc cggaatctgt tggccacgct cgcctcgacg atggcactga tcctggtgaa actctggtga tgcgtggccg tgttctcggc ggtaacggcg caccgctggc aaacgccctc gttgaagtct ggcacgctaa ccatttaggc aactatagct acttcgaccc gtcccagccg gccttcaacc ttcgtcgttc cattcgtact gacgcagaag gccgttacag cttccgttct gttctccctg taggctactc tgtgccgccg ggctctaaga ctgaacagct gttagatcag ttaggccgtc acggccaccg ccctgcccac attcacttct ttgtttctgc gccaggccac cgcaagctga ccacccagat taacatcgaa ggtgacccac acatctggga cgactttgca ttcgccaccc gtgaaggcct gatcccgaag attgcacagg cagagggtgc tcagggcaaa ccgtacggta ttgacggtcg tttcgccctg atcgacttcg acttcactct gacccgcgaa cgtggcgatg ttcctgctag cgaagtggaa cgtgttcgtt aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for BupsA.00117.a.D13.PD00170
PURIFICATION DATe: 9/16/2010
CONCENTRATION: 22.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNKE AIDALLKSFD DAATRPGNPR VRAIVNRIVK DLFYTIEDCD VQPSEFWTAL NYLNDAGKEF GLIAAGLGFE RFLDVRMDEA EAKAGLQGGT PRTIEGPLYV AGAPESVGHA RLDDGTDPGE TLVMRGRVLG GNGAPLANAL VEVWHANHLG NYSYFDPSQP AFNLRRSIRT DAEGRYSFRS VLPVGYSVPP GSKTEQLLDQ LGRHGHRPAH IHFFVSAPGH RKLTTQINIE GDPHIWDDFA FATREGLIPK IAQAEGAQGK PYGIDGRFAL IDFDFTLTRE RGDVPASEVE RVR
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaacaaagaa gcaattgacg ctctgctcaa gtccttcgat gacgctgcta ctcgtccggg caaccctcgt gttcgtgcaa tagttaaccg catcgttaaa gatctgttct acaccatcga agactgcgac gtgcagcctt ccgaattctg gactgccctg aactacctta acgatgcagg taaagaattc ggtctgatcg cagccggctt aggcttcgaa cgcttcctgg atgtgcgtat ggacgaagca gaagctaagg cgggcctcca gggcggcact ccgcgtacta ttgagggccc tctgtacgta gctggcgctc cggaatctgt tggccacgct cgcctcgacg atggcactga tcctggtgaa actctggtga tgcgtggccg tgttctcggc ggtaacggcg caccgctggc aaacgccctc gttgaagtct ggcacgctaa ccatttaggc aactatagct acttcgaccc gtcccagccg gccttcaacc ttcgtcgttc cattcgtact gacgcagaag gccgttacag cttccgttct gttctccctg taggctactc tgtgccgccg ggctctaaga ctgaacagct gttagatcag ttaggccgtc acggccaccg ccctgcccac attcacttct ttgtttctgc gccaggccac cgcaagctga ccacccagat taacatcgaa ggtgacccac acatctggga cgactttgca ttcgccaccc gtgaaggcct gatcccgaag attgcacagg cagagggtgc tcagggcaaa ccgtacggta ttgacggtcg tttcgccctg atcgacttcg acttcactct gacccgcgaa cgtggcgatg ttcctgctag cgaagtggaa cgtgttcgtt aa
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNKE AIDALLKSFD DAATRPGNPR VRAIVNRIVK DLFYTIEDCD VQPSEFWTAL NYLNDAGKEF GLIAAGLGFE RFLDVRMDEA EAKAGLQGGT PRTIEGPLYV AGAPESVGHA RLDDGTDPGE TLVMRGRVLG GNGAPLANAL VEVWHANHLG NYSYFDPSQP AFNLRRSIRT DAEGRYSFRS VLPVGYSVPP GSKTEQLLDQ LGRHGHRPAH IHFFVSAPGH RKLTTQINIE GDPHIWDDFA FATREGLIPK IAQAEGAQGK PYGIDGRFAL IDFDFTLTRE RGDVPASEVE RVR
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GAACAAAGAA GCAATTGACG CTCTGCTCAA GTCCTTCGAT GACGCTGCTA CTCGTCCGGG CAACCCTCGT GTTCGTGCAA TAGTTAACCG CATCGTTAAA GATCTGTTCT ACACCATCGA AGACTGCGAC GTGCAGCCTT CCGAATTCTG GACTGCCCTG AACTACCTTA ACGATGCAGG TAAAGAATTC GGTCTGATCG CAGCCGGCTT AGGCTTCGAA CGCTTCCTGG ATGTGCGTAT GGACGAAGCA GAAGCTAAGG CGGGCCTCCA GGGCGGCACT CCGCGTACTA TTGAGGGCCC TCTGTACGTA GCTGGCGCTC CGGAATCTGT TGGCCACGCT CGCCTCGACG ATGGCACTGA TCCTGGTGAA ACTCTGGTGA TGCGTGGCCG TGTTCTCGGC GGTAACGGCG CACCGCTGGC AAACGCCCTC GTTGAAGTCT GGCACGCTAA CCATTTAGGC AACTATAGCT ACTTCGACCC GTCCCAGCCG GCCTTCAACC TTCGTCGTTC CATTCGTACT GACGCAGAAG GCCGTTACAG CTTCCGTTCT GTTCTCCCTG TAGGCTACTC TGTGCCGCCG GGCTCTAAGA CTGAACAGCT GTTAGATCAG TTAGGCCGTC ACGGCCACCG CCCTGCCCAC ATTCACTTCT TTGTTTCTGC GCCAGGCCAC CGCAAGCTGA CCACCCAGAT TAACATCGAA GGTGACCCAC ACATCTGGGA CGACTTTGCA TTCGCCACCC GTGAAGGCCT GATCCCGAAG ATTGCACAGG CAGAGGGTGC TCAGGGCAAA CCGTACGGTA TTGACGGTCG TTTCGCCCTG ATCGACTTCG ACTTCACTCT GACCCGCGAA CGTGGCGATG TTCCTGCTAG CGAAGTGGAA CGTGTTCGTT AAAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT