BupsA.00114.a
phosphoglyceromutase
| CENTER ID: | BupsA.00114.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00114.a.A1.PS00007 | Full length( BupsA.00114.a ) | 1 | 249 | unavailable | |
| BupsA.00114.a.B1.PS00024 | Full length( BupsA.00114.a ) | 1 | 249 |
Structures
3EZN
DEPOSITED: 10/23/2008
DETERMINATION: XRay
CLONE: BupsA.00114.a.B1.GE25167
PROTEIN: BupsA.00114.a.B1.PS00024
3FDZ
DEPOSITED: 11/26/2008
DETERMINATION: XRay
CLONE: BupsA.00114.a.B1.GE25167
PROTEIN: BupsA.00114.a.B1.PS00024
3GP3
DEPOSITED: 3/20/2009
DETERMINATION: XRay
CLONE: BupsA.00114.a.B1.GE25167
PROTEIN: BupsA.00114.a.B1.PS00024
3GP5
DEPOSITED: 3/20/2009
DETERMINATION: XRay
CLONE: BupsA.00114.a.B1.GE25167
PROTEIN: BupsA.00114.a.B1.PS00024
3GW8
DEPOSITED: 3/31/2009
DETERMINATION: XRay
CLONE: BupsA.00114.a.B1.GE25167
PROTEIN: BupsA.00114.a.B1.PS00024
3LNT
DEPOSITED: 2/3/2010
DETERMINATION: XRay
CLONE: BupsA.00114.a.B1.GE25167
PROTEIN: BupsA.00114.a.B1.PS00024
Publications by SSGCID
An ensemble of structures of Burkholderia pseudomallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase.
Abendroth JA, Davies DR, Edwards TE, Hartley R, Hewitt SN, Kim H, Leonard J, Myler PJ, Staker BL, Stewart LJ, Rychel AL
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1044-50
PMID: 21904048; PMCID: PMC3169400
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127491 |
| PATRIC ID: | fig|320372.6.peg.675 |
| RefSeq: | YP_332076 |
| UniProt: | Q3JWH7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYKLVLIRHG ESTWNKENRF TGWVDVDLTE QGNREARQAG QLLKEAGYTF DIAYTSVLKR AIRTLWHVQD QMDLMYVPVV HSWRLNERHY GALSGLNKAE TAAKYGDEQV LVWRRSYDTP PPALEPGDER APYADPRYAK VPREQLPLTE CLKDTVARVL PLWNESIAPA VKAGKQVLIA AHGNSLRALI KYLDGISDAD IVGLNIPNGV PLVYELDESL TPIRHYYLGD QEAIAKAQAA VAQQGKSAA
NT Sequence
ATGTACAAGC TCGTTCTCAT CCGCCACGGC GAATCGACGT GGAACAAGGA AAACCGCTTC ACCGGCTGGG TCGATGTCGA CCTGACCGAA CAAGGCAATC GCGAAGCCCG GCAGGCGGGC CAGCTCCTGA AGGAAGCCGG CTACACGTTC GATATCGCAT ACACGTCGGT GCTCAAGCGC GCGATCCGCA CGCTCTGGCA CGTGCAGGAC CAGATGGATC TGATGTACGT GCCCGTCGTG CATTCGTGGC GCCTGAACGA GCGCCATTAC GGCGCGCTGT CGGGCCTGAA CAAGGCGGAG ACGGCCGCGA AGTACGGCGA CGAGCAGGTG CTCGTCTGGC GCCGCAGCTA CGACACGCCG CCGCCCGCGC TCGAGCCGGG CGACGAGCGC GCGCCGTACG CCGATCCGCG CTACGCGAAG GTGCCGCGCG AGCAGTTGCC GCTCACCGAG TGCCTGAAGG ACACGGTCGC GCGCGTGCTG CCGCTCTGGA ACGAATCGAT CGCGCCCGCG GTGAAGGCCG GCAAGCAGGT GCTGATCGCC GCGCACGGCA ACTCGCTGCG CGCGCTGATC AAGTATCTCG ACGGCATCTC CGACGCGGAC ATCGTCGGCC TGAACATCCC GAACGGCGTG CCGCTCGTCT ACGAGCTCGA CGAGAGCCTC ACGCCCATCC GGCACTACTA CCTCGGCGAT CAGGAGGCGA TCGCGAAGGC GCAGGCCGCC GTCGCGCAGC AGGGCAAGTC CGCGGCA
Details for BupsA.00114.a.A1.PS00007
| PURIFICATION DATe: | 5/6/2008 |
| CONCENTRATION: | 7.7mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYKLVLIRH GESTWNKENR FTGWVDVDLT EQGNREARQA GQLLKEAGYT FDIAYTSVLK RAIRTLWHVQ DQMDLMYVPV VHSWRLNERH YGALSGLNKA ETAAKYGDEQ VLVWRRSYDT PPPALEPGDE RAPYADPRYA KVPREQLPLT ECLKDTVARV LPLWNESIAP AVKAGKQVLI AAHGNSLRAL IKYLDGISDA DIVGLNIPNG VPLVYELDES LTPIRHYYLG DQEAIAKAQA AVAQQGKSAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTACAAGCTC GTTCTCATCC GCCACGGCGA ATCGACGTGG AACAAGGAAA ACCGCTTCAC CGGCTGGGTC GATGTCGACC TGACCGAACA AGGCAATCGC GAAGCCCGGC AGGCGGGCCA GCTCCTGAAG GAAGCCGGCT ACACGTTCGA TATCGCATAC ACGTCGGTGC TCAAGCGCGC GATCCGCACG CTCTGGCACG TGCAGGACCA GATGGATCTG ATGTACGTGC CCGTCGTGCA TTCGTGGCGC CTGAACGAGC GCCATTACGG CGCGCTGTCG GGCCTGAACA AGGCGGAGAC GGCCGCGAAG TACGGCGACG AGCAGGTGCT CGTCTGGCGC CGCAGCTACG ACACGCCGCC GCCCGCGCTC GAGCCGGGCG ACGAGCGCGC GCCGTACGCC GATCCGCGCT ACGCGAAGGT GCCGCGCGAG CAGTTGCCGC TCACCGAGTG CCTGAAGGAC ACGGTCGCGC GCGTGCTGCC GCTCTGGAAC GAATCGATCG CGCCCGCGGT GAAGGCCGGC AAGCAGGTGC TGATCGCCGC GCACGGCAAC TCGCTGCGCG CGCTGATCAA GTATCTCGAC GGCATCTCCG ACGCGGACAT CGTCGGCCTG AACATCCCGA ACGGCGTGCC GCTCGTCTAC GAGCTCGACG AGAGCCTCAC GCCCATCCGG CACTACTACC TCGGCGATCA GGAGGCGATC GCGAAGGCGC AGGCCGCCGT CGCGCAGCAG GGCAAGTCCG CGGCATAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BupsA.00114.a.B1.PS00024
| PURIFICATION DATe: | 6/17/2008 |
| CONCENTRATION: | 11.66mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 150µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMY KLVLIRHGES TWNKENRFTG WVDVDLTEQG NREARQAGQL LKEAGYTFDI AYTSVLKRAI RTLWHVQDQM DLMYVPVVHS WRLNERHYGA LSGLNKAETA AKYGDEQVLV WRRSYDTPPP ALEPGDERAP YADPRYAKVP REQLPLTECL KDTVARVLPL WNESIAPAVK AGKQVLIAAH GNSLRALIKY LDGISDADIV GLNIPNGVPL VYELDESLTP IRHYYLGDQE AIAKAQAAVA QQGKSAA
Validated NT Sequence
tttaagaagg agatatacca tggctcacca ccaccaccac catatgtaca agctcgttct catccgccac ggcgaatcga cgtggaacaa ggaaaaccgc ttcaccggct gggtcgatgt cgacctgacc gaacaaggca atcgcgaagc ccggcaggcg ggccagctcc tgaaggaagc cggctacacg ttcgatatcg catacacgtc ggtgctcaag cgcgcgatcc gcacgctctg gcacgtgcag gaccagatgg atctgatgta cgtgcccgtc gtgcattcgt ggcgcctgaa cgagcgccat tacggcgcgc tgtcgggcct gaacaaggcg gagacggccg cgaagtacgg cgacgagcag gtgctcgtct ggcgccgcag ctacgacacg ccgccgcccg cgctcgagcc gggcgacgag cgcgcgccgt acgccgatcc gcgctacgcg aaggtgccgc gcgagcagtt gccgctcacc gagtgcctga aggacacggt cgcgcgcgtg ctgccgctct ggaacgaatc gatcgcgccc gcggtgaagg ccggcaagca ggtgctgatc gccgcgcacg gcaactcgct gcgcgcgctg atcaagtatc tcgacggcat ctccgacgcg gacatcgtcg gcctgaacat cccgaacggc gtgccgctcg tctacgagct cgacgagagc ctcacgccca tccggcacta ctacctcggc gatcaggagg cgatcgcgaa ggcgcaggcc gccgtcgcgc agcagggcaa gtccgcggca taagtgagta agat
Expressed Protein Sequence
MAHHHHHHMY KLVLIRHGES TWNKENRFTG WVDVDLTEQG NREARQAGQL LKEAGYTFDI AYTSVLKRAI RTLWHVQDQM DLMYVPVVHS WRLNERHYGA LSGLNKAETA AKYGDEQVLV WRRSYDTPPP ALEPGDERAP YADPRYAKVP REQLPLTECL KDTVARVLPL WNESIAPAVK AGKQVLIAAH GNSLRALIKY LDGISDADIV GLNIPNGVPL VYELDESLTP IRHYYLGDQE AIAKAQAAVA QQGKSAA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTACA AGCTCGTTCT CATCCGCCAC GGCGAATCGA CGTGGAACAA GGAAAACCGC TTCACCGGCT GGGTCGATGT CGACCTGACC GAACAAGGCA ATCGCGAAGC CCGGCAGGCG GGCCAGCTCC TGAAGGAAGC CGGCTACACG TTCGATATCG CATACACGTC GGTGCTCAAG CGCGCGATCC GCACGCTCTG GCACGTGCAG GACCAGATGG ATCTGATGTA CGTGCCCGTC GTGCATTCGT GGCGCCTGAA CGAGCGCCAT TACGGCGCGC TGTCGGGCCT GAACAAGGCG GAGACGGCCG CGAAGTACGG CGACGAGCAG GTGCTCGTCT GGCGCCGCAG CTACGACACG CCGCCGCCCG CGCTCGAGCC GGGCGACGAG CGCGCGCCGT ACGCCGATCC GCGCTACGCG AAGGTGCCGC GCGAGCAGTT GCCGCTCACC GAGTGCCTGA AGGACACGGT CGCGCGCGTG CTGCCGCTCT GGAACGAATC GATCGCGCCC GCGGTGAAGG CCGGCAAGCA GGTGCTGATC GCCGCGCACG GCAACTCGCT GCGCGCGCTG ATCAAGTATC TCGACGGCAT CTCCGACGCG GACATCGTCG GCCTGAACAT CCCGAACGGC GTGCCGCTCG TCTACGAGCT CGACGAGAGC CTCACGCCCA TCCGGCACTA CTACCTCGGC GATCAGGAGG CGATCGCGAA GGCGCAGGCC GCCGTCGCGC AGCAGGGCAA GTCCGCGGCA TAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT