BupsA.00113.a

3-oxoacyl-(acyl carrier protein) synthase II

CENTER ID: BupsA.00113.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00113.a.A1.GE25434 Full length( BupsA.00113.a ) 1 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00113.a.A1.PS00065 Full length( BupsA.00113.a ) 1 412
BupsA.00113.a.A1.PS00353 Full length( BupsA.00113.a ) 1 412
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126985
PATRIC ID: fig|320372.6.peg.3034
RefSeq: YP_334284.1
UniProt: Q3JQ69
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRRRVVVTG LGLISPVGNN VADGWANLVA GKSGIVNITK FDATNFSTRF AGEVKGFNVE EYIPAKEARH MDTFIHYGIA AGMQAMQDSG FQVTDENAER VGVVVGSGIG GLPMIEVTQT ELLNRGPRRI SPFFVPASII NMISGHLSIK FGIKGPNLAI VTACTTGLHC IGEAARLIEY GDADVMIAGG AEATVSPLGI GGFAAARALS QRNDDPATAS RPWDKDRDGF VLGEGAGVMV LEEYEHAKAR GAKIYAEVAG YGMSGDAYHM TAPLENGDGA RRCMLAALRN AGVNADQVNY LNAHGTSTPL GDLAETVAIK RAFGDHSKDI VVNSTKSMTG HLLGGAGGLE SVFTVLAVHN QVSPPTINIF NQDPECDLDY CANEAREMKI DVALKNSFGF GGTNGTLVFK RA
NT Sequence
GTGAGCCGCC GTCGTGTTGT TGTTACAGGC CTTGGGCTGA TTTCGCCTGT TGGCAATAAT GTTGCCGACG GCTGGGCCAA TCTCGTGGCC GGCAAGTCCG GCATCGTCAA TATCACGAAG TTCGACGCGA CGAACTTCTC GACCCGCTTC GCGGGCGAGG TGAAGGGCTT CAACGTCGAG GAGTACATCC CGGCGAAGGA AGCGCGCCAC ATGGATACGT TCATCCATTA CGGCATCGCG GCCGGCATGC AGGCGATGCA GGACAGCGGC TTCCAGGTCA CCGACGAAAA CGCGGAGCGC GTCGGCGTCG TGGTCGGCTC GGGCATCGGC GGGCTGCCGA TGATCGAAGT CACGCAGACC GAGCTGCTCA ACCGCGGCCC GCGCCGGATC TCGCCGTTCT TCGTGCCGGC GTCGATCATC AACATGATCT CCGGCCACCT GTCGATCAAG TTCGGCATCA AGGGCCCGAA TCTCGCGATC GTCACCGCGT GCACGACCGG CCTGCACTGC ATCGGCGAGG CGGCGCGTCT GATCGAATAC GGCGACGCCG ACGTGATGAT CGCGGGCGGC GCGGAAGCGA CCGTGTCGCC GCTCGGCATC GGCGGCTTCG CGGCGGCGCG CGCGCTGTCG CAGCGCAACG ACGATCCGGC GACGGCGAGC CGTCCGTGGG ACAAGGATCG CGACGGCTTC GTGCTCGGCG AGGGCGCGGG CGTGATGGTG CTCGAGGAGT ACGAGCACGC GAAGGCGCGC GGCGCGAAGA TCTACGCGGA AGTCGCGGGC TACGGGATGA GCGGCGACGC GTACCACATG ACGGCGCCGC TCGAGAACGG CGACGGCGCA CGCCGCTGCA TGCTGGCCGC GCTGCGCAAC GCGGGCGTGA ACGCCGATCA GGTGAATTAC CTGAACGCGC ACGGCACGTC GACGCCGCTC GGCGATCTGG CCGAGACCGT CGCGATCAAG CGCGCGTTCG GCGATCACTC GAAGGACATC GTCGTCAACT CGACCAAGTC GATGACGGGG CACTTGCTCG GCGGCGCCGG CGGGCTCGAA TCGGTGTTCA CGGTCCTTGC CGTCCACAAC CAGGTGTCGC CGCCGACGAT CAACATCTTC AACCAGGACC CCGAGTGCGA TCTCGACTAT TGCGCGAACG AAGCGCGCGA GATGAAGATC GACGTCGCGT TGAAGAACTC CTTCGGCTTC GGCGGGACTA ACGGCACGCT GGTCTTCAAG CGCGCC
Details for BupsA.00113.a.A1.GE25434
HARVESTED ON: 6/30/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRRRVVV TGLGLISPVG NNVADGWANL VAGKSGIVNI TKFDATNFST RFAGEVKGFN VEEYIPAKEA RHMDTFIHYG IAAGMQAMQD SGFQVTDENA ERVGVVVGSG IGGLPMIEVT QTELLNRGPR RISPFFVPAS IINMISGHLS IKFGIKGPNL AIVTACTTGL HCIGEAARLI EYGDADVMIA GGAEATVSPL GIGGFAAARA LSQRNDDPAT ASRPWDKDRD GFVLGEGAGV MVLEEYEHAK ARGAKIYAEV AGYGMSGDAY HMTAPLENGD GARRCMLAAL RNAGVNADQV NYLNAHGTST PLGDLAETVA IKRAFGDHSK DIVVNSTKSM TGHLLGGAGG LESVFTVLAV HNQVSPPTIN IFNQDPECDL DYCANEAREM KIDVALKNSF GFGGTNGTLV FKRA
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagc cgccgtcgtg ttgttgttac aggccttggg ctgatttcgc ctgttggcaa taatgttgcc gacggctggg ccaatctcgt ggccggcaag tccggcatcg tcaatatcac gaagttcgac gcgacgaact tctcgacccg cttcgcgggc gaggtgaagg gcttcaacgt cgaggagtac atcccggcga aggaagcgcg ccacatggat acgttcatcc attacggcat cgcggccggc atgcaggcga tgcaggacag cggcttccag gtcaccgacg aaaacgcgga gcgcgtcggc gtcgtggtcg gctcgggcat cggcgggctg ccgatgatcg aagtcacgca gaccgagctg ctcaaccgcg gcccgcgccg gatctcgccg ttcttcgtgc cggcgtcgat catcaacatg atctccggcc acctgtcgat caagttcggc atcaagggcc cgaatctcgc gatcgtcacc gcgtgcacga ccggcctgca ctgcatcggc gaggcggcgc gtctgatcga atacggcgac gccgacgtga tgatcgcggg cggcgcggaa gcgaccgtgt cgccgctcgg catcggcggc ttcgcggcgg cgcgcgcgct gtcgcagcgc aacgacgatc cggcgacggc gagccgtccg tgggacaagg atcgcgacgg cttcgtgctc ggcgagggcg cgggcgtgat ggtgctcgag gagtacgagc acgcgaaggc gcgcggcgcg aagatctacg cggaagtcgc gggctacggg atgagcggcg acgcgtacca catgacggcg ccgctcgaga acggcgacgg cgcacgccgc tgcatgctgg ccgcgctgcg caacgcgggc gtgaacgccg atcaggtgaa ttacctgaac gcgcacggca cgtcgacgcc gctcggcgat ctggccgaga ccgtcgcgat caagcgcgcg ttcggcgatc actcgaagga catcgtcgtc aactcgacca agtcgatgac ggggcacttg ctcggcggcg ccggcgggct cgaatcggtg ttcacggtcc ttgccgtcca caaccaggtg tcgccgccga cgatcaacat cttcaaccag gaccccgagt gcgatctcga ctattgcgcg aacgaagcgc gcgagatgaa gatcgacgtc gcgttgaaga actccttcgg cttcggcggg actaacggca cgctggtctt caagcgcgcc taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRRRVVV TGLGLISPVG NNVADGWANL VAGKSGIVNI TKFDATNFST RFAGEVKGFN VEEYIPAKEA RHMDTFIHYG IAAGMQAMQD SGFQVTDENA ERVGVVVGSG IGGLPMIEVT QTELLNRGPR RISPFFVPAS IINMISGHLS IKFGIKGPNL AIVTACTTGL HCIGEAARLI EYGDADVMIA GGAEATVSPL GIGGFAAARA LSQRNDDPAT ASRPWDKDRD GFVLGEGAGV MVLEEYEHAK ARGAKIYAEV AGYGMSGDAY HMTAPLENGD GARRCMLAAL RNAGVNADQV NYLNAHGTST PLGDLAETVA IKRAFGDHSK DIVVNSTKSM TGHLLGGAGG LESVFTVLAV HNQVSPPTIN IFNQDPECDL DYCANEAREM KIDVALKNSF GFGGTNGTLV FKRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagccgc cgtcgtgttg ttgttacagg ccttgggctg atttcgcctg ttggcaataa tgttgccgac ggctgggcca atctcgtggc cggcaagtcc ggcatcgtca atatcacgaa gttcgacgcg acgaacttct cgacccgctt cgcgggcgag gtgaagggct tcaacgtcga ggagtacatc ccggcgaagg aagcgcgcca catggatacg ttcatccatt acggcatcgc ggccggcatg caggcgatgc aggacagcgg cttccaggtc accgacgaaa acgcggagcg cgtcggcgtc gtggtcggct cgggcatcgg cgggctgccg atgatcgaag tcacgcagac cgagctgctc aaccgcggcc cgcgccggat ctcgccgttc ttcgtgccgg cgtcgatcat caacatgatc tccggccacc tgtcgatcaa gttcggcatc aagggcccga atctcgcgat cgtcaccgcg tgcacgaccg gcctgcactg catcggcgag gcggcgcgtc tgatcgaata cggcgacgcc gacgtgatga tcgcgggcgg cgcggaagcg accgtgtcgc cgctcggcat cggcggcttc gcggcggcgc gcgcgctgtc gcagcgcaac gacgatccgg cgacggcgag ccgtccgtgg gacaaggatc gcgacggctt cgtgctcggc gagggcgcgg gcgtgatggt gctcgaggag tacgagcacg cgaaggcgcg cggcgcgaag atctacgcgg aagtcgcggg ctacgggatg agcggcgacg cgtaccacat gacggcgccg ctcgagaacg gcgacggcgc acgccgctgc atgctggccg cgctgcgcaa cgcgggcgtg aacgccgatc aggtgaatta cctgaacgcg cacggcacgt cgacgccgct cggcgatctg gccgagaccg tcgcgatcaa gcgcgcgttc ggcgatcact cgaaggacat cgtcgtcaac tcgaccaagt cgatgacggg gcacttgctc ggcggcgccg gcgggctcga atcggtgttc acggtccttg ccgtccacaa ccaggtgtcg ccgccgacga tcaacatctt caaccaggac cccgagtgcg atctcgacta ttgcgcgaac gaagcgcgcg agatgaagat cgacgtcgcg ttgaagaact ccttcggctt cggcgggact aacggcacgc tggtcttcaa gcgcgccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BupsA.00113.a.A1.PS00065
PURIFICATION DATe: 8/21/2008
CONCENTRATION: 35.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRRRVVV TGLGLISPVG NNVADGWANL VAGKSGIVNI TKFDATNFST RFAGEVKGFN VEEYIPAKEA RHMDTFIHYG IAAGMQAMQD SGFQVTDENA ERVGVVVGSG IGGLPMIEVT QTELLNRGPR RISPFFVPAS IINMISGHLS IKFGIKGPNL AIVTACTTGL HCIGEAARLI EYGDADVMIA GGAEATVSPL GIGGFAAARA LSQRNDDPAT ASRPWDKDRD GFVLGEGAGV MVLEEYEHAK ARGAKIYAEV AGYGMSGDAY HMTAPLENGD GARRCMLAAL RNAGVNADQV NYLNAHGTST PLGDLAETVA IKRAFGDHSK DIVVNSTKSM TGHLLGGAGG LESVFTVLAV HNQVSPPTIN IFNQDPECDL DYCANEAREM KIDVALKNSF GFGGTNGTLV FKRA
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagc cgccgtcgtg ttgttgttac aggccttggg ctgatttcgc ctgttggcaa taatgttgcc gacggctggg ccaatctcgt ggccggcaag tccggcatcg tcaatatcac gaagttcgac gcgacgaact tctcgacccg cttcgcgggc gaggtgaagg gcttcaacgt cgaggagtac atcccggcga aggaagcgcg ccacatggat acgttcatcc attacggcat cgcggccggc atgcaggcga tgcaggacag cggcttccag gtcaccgacg aaaacgcgga gcgcgtcggc gtcgtggtcg gctcgggcat cggcgggctg ccgatgatcg aagtcacgca gaccgagctg ctcaaccgcg gcccgcgccg gatctcgccg ttcttcgtgc cggcgtcgat catcaacatg atctccggcc acctgtcgat caagttcggc atcaagggcc cgaatctcgc gatcgtcacc gcgtgcacga ccggcctgca ctgcatcggc gaggcggcgc gtctgatcga atacggcgac gccgacgtga tgatcgcggg cggcgcggaa gcgaccgtgt cgccgctcgg catcggcggc ttcgcggcgg cgcgcgcgct gtcgcagcgc aacgacgatc cggcgacggc gagccgtccg tgggacaagg atcgcgacgg cttcgtgctc ggcgagggcg cgggcgtgat ggtgctcgag gagtacgagc acgcgaaggc gcgcggcgcg aagatctacg cggaagtcgc gggctacggg atgagcggcg acgcgtacca catgacggcg ccgctcgaga acggcgacgg cgcacgccgc tgcatgctgg ccgcgctgcg caacgcgggc gtgaacgccg atcaggtgaa ttacctgaac gcgcacggca cgtcgacgcc gctcggcgat ctggccgaga ccgtcgcgat caagcgcgcg ttcggcgatc actcgaagga catcgtcgtc aactcgacca agtcgatgac ggggcacttg ctcggcggcg ccggcgggct cgaatcggtg ttcacggtcc ttgccgtcca caaccaggtg tcgccgccga cgatcaacat cttcaaccag gaccccgagt gcgatctcga ctattgcgcg aacgaagcgc gcgagatgaa gatcgacgtc gcgttgaaga actccttcgg cttcggcggg actaacggca cgctggtctt caagcgcgcc taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRRRVVV TGLGLISPVG NNVADGWANL VAGKSGIVNI TKFDATNFST RFAGEVKGFN VEEYIPAKEA RHMDTFIHYG IAAGMQAMQD SGFQVTDENA ERVGVVVGSG IGGLPMIEVT QTELLNRGPR RISPFFVPAS IINMISGHLS IKFGIKGPNL AIVTACTTGL HCIGEAARLI EYGDADVMIA GGAEATVSPL GIGGFAAARA LSQRNDDPAT ASRPWDKDRD GFVLGEGAGV MVLEEYEHAK ARGAKIYAEV AGYGMSGDAY HMTAPLENGD GARRCMLAAL RNAGVNADQV NYLNAHGTST PLGDLAETVA IKRAFGDHSK DIVVNSTKSM TGHLLGGAGG LESVFTVLAV HNQVSPPTIN IFNQDPECDL DYCANEAREM KIDVALKNSF GFGGTNGTLV FKRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCCGC CGTCGTGTTG TTGTTACAGG CCTTGGGCTG ATTTCGCCTG TTGGCAATAA TGTTGCCGAC GGCTGGGCCA ATCTCGTGGC CGGCAAGTCC GGCATCGTCA ATATCACGAA GTTCGACGCG ACGAACTTCT CGACCCGCTT CGCGGGCGAG GTGAAGGGCT TCAACGTCGA GGAGTACATC CCGGCGAAGG AAGCGCGCCA CATGGATACG TTCATCCATT ACGGCATCGC GGCCGGCATG CAGGCGATGC AGGACAGCGG CTTCCAGGTC ACCGACGAAA ACGCGGAGCG CGTCGGCGTC GTGGTCGGCT CGGGCATCGG CGGGCTGCCG ATGATCGAAG TCACGCAGAC CGAGCTGCTC AACCGCGGCC CGCGCCGGAT CTCGCCGTTC TTCGTGCCGG CGTCGATCAT CAACATGATC TCCGGCCACC TGTCGATCAA GTTCGGCATC AAGGGCCCGA ATCTCGCGAT CGTCACCGCG TGCACGACCG GCCTGCACTG CATCGGCGAG GCGGCGCGTC TGATCGAATA CGGCGACGCC GACGTGATGA TCGCGGGCGG CGCGGAAGCG ACCGTGTCGC CGCTCGGCAT CGGCGGCTTC GCGGCGGCGC GCGCGCTGTC GCAGCGCAAC GACGATCCGG CGACGGCGAG CCGTCCGTGG GACAAGGATC GCGACGGCTT CGTGCTCGGC GAGGGCGCGG GCGTGATGGT GCTCGAGGAG TACGAGCACG CGAAGGCGCG CGGCGCGAAG ATCTACGCGG AAGTCGCGGG CTACGGGATG AGCGGCGACG CGTACCACAT GACGGCGCCG CTCGAGAACG GCGACGGCGC ACGCCGCTGC ATGCTGGCCG CGCTGCGCAA CGCGGGCGTG AACGCCGATC AGGTGAATTA CCTGAACGCG CACGGCACGT CGACGCCGCT CGGCGATCTG GCCGAGACCG TCGCGATCAA GCGCGCGTTC GGCGATCACT CGAAGGACAT CGTCGTCAAC TCGACCAAGT CGATGACGGG GCACTTGCTC GGCGGCGCCG GCGGGCTCGA ATCGGTGTTC ACGGTCCTTG CCGTCCACAA CCAGGTGTCG CCGCCGACGA TCAACATCTT CAACCAGGAC CCCGAGTGCG ATCTCGACTA TTGCGCGAAC GAAGCGCGCG AGATGAAGAT CGACGTCGCG TTGAAGAACT CCTTCGGCTT CGGCGGGACT AACGGCACGC TGGTCTTCAA GCGCGCCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for BupsA.00113.a.A1.PS00353
PURIFICATION DATe: 9/14/2009
CONCENTRATION: 19.81mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 150µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRRRVVV TGLGLISPVG NNVADGWANL VAGKSGIVNI TKFDATNFST RFAGEVKGFN VEEYIPAKEA RHMDTFIHYG IAAGMQAMQD SGFQVTDENA ERVGVVVGSG IGGLPMIEVT QTELLNRGPR RISPFFVPAS IINMISGHLS IKFGIKGPNL AIVTACTTGL HCIGEAARLI EYGDADVMIA GGAEATVSPL GIGGFAAARA LSQRNDDPAT ASRPWDKDRD GFVLGEGAGV MVLEEYEHAK ARGAKIYAEV AGYGMSGDAY HMTAPLENGD GARRCMLAAL RNAGVNADQV NYLNAHGTST PLGDLAETVA IKRAFGDHSK DIVVNSTKSM TGHLLGGAGG LESVFTVLAV HNQVSPPTIN IFNQDPECDL DYCANEAREM KIDVALKNSF GFGGTNGTLV FKRA
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagc cgccgtcgtg ttgttgttac aggccttggg ctgatttcgc ctgttggcaa taatgttgcc gacggctggg ccaatctcgt ggccggcaag tccggcatcg tcaatatcac gaagttcgac gcgacgaact tctcgacccg cttcgcgggc gaggtgaagg gcttcaacgt cgaggagtac atcccggcga aggaagcgcg ccacatggat acgttcatcc attacggcat cgcggccggc atgcaggcga tgcaggacag cggcttccag gtcaccgacg aaaacgcgga gcgcgtcggc gtcgtggtcg gctcgggcat cggcgggctg ccgatgatcg aagtcacgca gaccgagctg ctcaaccgcg gcccgcgccg gatctcgccg ttcttcgtgc cggcgtcgat catcaacatg atctccggcc acctgtcgat caagttcggc atcaagggcc cgaatctcgc gatcgtcacc gcgtgcacga ccggcctgca ctgcatcggc gaggcggcgc gtctgatcga atacggcgac gccgacgtga tgatcgcggg cggcgcggaa gcgaccgtgt cgccgctcgg catcggcggc ttcgcggcgg cgcgcgcgct gtcgcagcgc aacgacgatc cggcgacggc gagccgtccg tgggacaagg atcgcgacgg cttcgtgctc ggcgagggcg cgggcgtgat ggtgctcgag gagtacgagc acgcgaaggc gcgcggcgcg aagatctacg cggaagtcgc gggctacggg atgagcggcg acgcgtacca catgacggcg ccgctcgaga acggcgacgg cgcacgccgc tgcatgctgg ccgcgctgcg caacgcgggc gtgaacgccg atcaggtgaa ttacctgaac gcgcacggca cgtcgacgcc gctcggcgat ctggccgaga ccgtcgcgat caagcgcgcg ttcggcgatc actcgaagga catcgtcgtc aactcgacca agtcgatgac ggggcacttg ctcggcggcg ccggcgggct cgaatcggtg ttcacggtcc ttgccgtcca caaccaggtg tcgccgccga cgatcaacat cttcaaccag gaccccgagt gcgatctcga ctattgcgcg aacgaagcgc gcgagatgaa gatcgacgtc gcgttgaaga actccttcgg cttcggcggg actaacggca cgctggtctt caagcgcgcc taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRRRVVV TGLGLISPVG NNVADGWANL VAGKSGIVNI TKFDATNFST RFAGEVKGFN VEEYIPAKEA RHMDTFIHYG IAAGMQAMQD SGFQVTDENA ERVGVVVGSG IGGLPMIEVT QTELLNRGPR RISPFFVPAS IINMISGHLS IKFGIKGPNL AIVTACTTGL HCIGEAARLI EYGDADVMIA GGAEATVSPL GIGGFAAARA LSQRNDDPAT ASRPWDKDRD GFVLGEGAGV MVLEEYEHAK ARGAKIYAEV AGYGMSGDAY HMTAPLENGD GARRCMLAAL RNAGVNADQV NYLNAHGTST PLGDLAETVA IKRAFGDHSK DIVVNSTKSM TGHLLGGAGG LESVFTVLAV HNQVSPPTIN IFNQDPECDL DYCANEAREM KIDVALKNSF GFGGTNGTLV FKRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCCGC CGTCGTGTTG TTGTTACAGG CCTTGGGCTG ATTTCGCCTG TTGGCAATAA TGTTGCCGAC GGCTGGGCCA ATCTCGTGGC CGGCAAGTCC GGCATCGTCA ATATCACGAA GTTCGACGCG ACGAACTTCT CGACCCGCTT CGCGGGCGAG GTGAAGGGCT TCAACGTCGA GGAGTACATC CCGGCGAAGG AAGCGCGCCA CATGGATACG TTCATCCATT ACGGCATCGC GGCCGGCATG CAGGCGATGC AGGACAGCGG CTTCCAGGTC ACCGACGAAA ACGCGGAGCG CGTCGGCGTC GTGGTCGGCT CGGGCATCGG CGGGCTGCCG ATGATCGAAG TCACGCAGAC CGAGCTGCTC AACCGCGGCC CGCGCCGGAT CTCGCCGTTC TTCGTGCCGG CGTCGATCAT CAACATGATC TCCGGCCACC TGTCGATCAA GTTCGGCATC AAGGGCCCGA ATCTCGCGAT CGTCACCGCG TGCACGACCG GCCTGCACTG CATCGGCGAG GCGGCGCGTC TGATCGAATA CGGCGACGCC GACGTGATGA TCGCGGGCGG CGCGGAAGCG ACCGTGTCGC CGCTCGGCAT CGGCGGCTTC GCGGCGGCGC GCGCGCTGTC GCAGCGCAAC GACGATCCGG CGACGGCGAG CCGTCCGTGG GACAAGGATC GCGACGGCTT CGTGCTCGGC GAGGGCGCGG GCGTGATGGT GCTCGAGGAG TACGAGCACG CGAAGGCGCG CGGCGCGAAG ATCTACGCGG AAGTCGCGGG CTACGGGATG AGCGGCGACG CGTACCACAT GACGGCGCCG CTCGAGAACG GCGACGGCGC ACGCCGCTGC ATGCTGGCCG CGCTGCGCAA CGCGGGCGTG AACGCCGATC AGGTGAATTA CCTGAACGCG CACGGCACGT CGACGCCGCT CGGCGATCTG GCCGAGACCG TCGCGATCAA GCGCGCGTTC GGCGATCACT CGAAGGACAT CGTCGTCAAC TCGACCAAGT CGATGACGGG GCACTTGCTC GGCGGCGCCG GCGGGCTCGA ATCGGTGTTC ACGGTCCTTG CCGTCCACAA CCAGGTGTCG CCGCCGACGA TCAACATCTT CAACCAGGAC CCCGAGTGCG ATCTCGACTA TTGCGCGAAC GAAGCGCGCG AGATGAAGAT CGACGTCGCG TTGAAGAACT CCTTCGGCTT CGGCGGGACT AACGGCACGC TGGTCTTCAA GCGCGCCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA