BupsA.00112.a
3-methyl-2-oxobutanoate hydroxymethyltransferase
| CENTER ID: | BupsA.00112.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00112.a.B1.GE25166 | Full length( BupsA.00112.a ) | 1 | 260 | ||
| BupsA.00112.a.B1.GE25166 | Full length( BupsA.00112.a ) | 1 | 260 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00112.a.B1.PS00023 | Full length( BupsA.00112.a ) | 1 | 260 |
Structures
3EZ4
DEPOSITED: 10/22/2008
DETERMINATION: XRay
CLONE: BupsA.00112.a.B1.GE25166
PROTEIN: BupsA.00112.a.B1.PS00023
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129046 |
| PATRIC ID: | fig|320372.6.peg.3456 |
| RefSeq: | YP_334688 |
| UniProt: | Q3JP15 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVPKLQAMR EAGEKIAMLT SYDASFAALL DRANVDVQLI GDSLGNVLQG QATTLPVTLD DIAYHTACVA RAQPRGLVVA DLPFGTYGTP ADAFASAVKL MRAGAQMVKL EGGEWLAETV RFLVERAVPV CAHVGLTPQS VHAFGGFKVQ GKTEAGAAQL LRDARAVEEA GAQLIVLEAV PTLVAAEVTR ELSIPTIGIG AGAECSGQVL VLHDMLGVFP GKRPRFVKDF MQGQPSIFAA VEAYVRAVKD GSFPGPEHSF
NT Sequence
GTGACGGTAC CCAAGCTGCA GGCGATGCGG GAAGCCGGCG AGAAGATCGC GATGCTCACC AGCTACGACG CAAGCTTCGC CGCCCTCCTC GATCGCGCGA ACGTCGACGT GCAACTGATC GGCGATTCGC TCGGCAACGT GCTGCAGGGC CAGGCGACGA CGCTGCCCGT CACGCTCGAC GACATCGCGT ATCACACCGC ATGCGTCGCG CGCGCGCAGC CGCGCGGGCT CGTCGTCGCG GACCTGCCGT TCGGCACCTA CGGCACGCCC GCCGACGCAT TCGCGAGCGC GGTGAAGCTG ATGCGCGCGG GCGCGCAGAT GGTCAAGCTC GAAGGCGGCG AATGGCTCGC CGAAACGGTG CGCTTCCTCG TCGAGCGGGC CGTGCCCGTG TGCGCGCACG TCGGCCTCAC GCCGCAGTCG GTGCACGCGT TCGGCGGCTT CAAGGTGCAA GGCAAGACGG AAGCAGGCGC CGCGCAATTG CTGCGCGATG CGCGCGCGGT CGAGGAAGCC GGCGCGCAGC TCATCGTGCT CGAGGCGGTG CCCACGCTCG TCGCGGCCGA AGTCACGCGC GAGCTGTCGA TCCCGACGAT CGGCATCGGC GCGGGCGCCG AATGCTCGGG CCAGGTACTC GTGCTGCACG ACATGCTCGG CGTGTTCCCC GGCAAGCGGC CGCGCTTCGT CAAGGATTTC ATGCAGGGGC AGCCGAGCAT CTTCGCAGCG GTCGAGGCCT ACGTGCGCGC GGTGAAGGAC GGCAGCTTCC CCGGGCCCGA GCATTCGTTC
Details for BupsA.00112.a.B1.GE25166
| HARVESTED ON: | 4/7/2008 |
| SEQUENCED ON: | 8/15/2008 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | failed alignment |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV TVPKLQAMRE AGEKIAMLTS YDASFAALLD RANVDVQLIG DSLGNVLQGQ ATTLPVTLDD IAYHTACVAR AQPRGLVVAD LPFGTYGTPA DAFASAVKLM RAGAQMVKLE GGEWLAETVR FLVERAVPVC AHVGLTPQSV HAFGGFKVQG KTEAGAAQLL RDARAVEEAG AQLIVLEAVP TLVAAEVTRE LSIPTIGIGA GAECSGQVLV LHDMLGVFPG KRPRFVKDFM QGQPSIFAAV EAYVRAVKDG SFPGPEHSF
Validated NT Sequence
atggctcacc accaccacca ccatatggtg acggtaccca agctgcaggc gatgcgggaa gccggcgaga agatcgcgat gctcaccagc tacgacgcaa gcttcgccgc cctcctcgat cgcgcgaacg tcgacgtgca actgatcggc gattcgctcg gcaacgtgct gcagggccag gcgacgacgc tgcccgtcac gctcgacgac atcgcgtatc acaccgcatg cgtcgcgcgc gcgcagccgc gcgggctcgt cgtcgcggac ctgccgttcg gcacctacgg cacgcccgcc gacgcattcg cgagcgcggt gaagctgatg cgcgcgggcg cgcagatggt caagctcgaa ggcggcgaat ggctcgccga aacggtgcgc ttcctcgtcg agcgggccgt gcccgtgtgc gcgcacgtcg gcctcacgcc gcagtcggtg cacgcgttcg gcggcttcaa ggtgcaaggc aagacggaag caggcgccgc gcaattgctg cgcgatgcgc gcgcggtcga ggaagccggc gcgcagctca tcgtgctcga ggcggtgccc acgctcgtcg cggccgaagt cacgcgcgag ctgtcgatcc cgacgatcgg catcggcgcg ggcgccgaat gctcgggcca ggtactcgtg ctgcacgaca tgctcggcgt gttccccggc aagcggccgc gcttcgtcaa ggatttcatg caggggcagc cgagcatctt cgcagcggtc gaggcctacg tgcgcgcggt gaaggacggc agcttccccg ggcccgagca ttcgttctaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMV TVPKLQAMRE AGEKIAMLTS YDASFAALLD RANVDVQLIG DSLGNVLQGQ ATTLPVTLDD IAYHTACVAR AQPRGLVVAD LPFGTYGTPA DAFASAVKLM RAGAQMVKLE GGEWLAETVR FLVERAVPVC AHVGLTPQSV HAFGGFKVQG KTEAGAAQLL RDARAVEEAG AQLIVLEAVP TLVAAEVTRE LSIPTIGIGA GAECSGQVLV LHDMLGVFPG KRPRFVKDFM QGQPSIFAAV EAYVRAVKDG SFPGPEHSF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga cggtacccaa gctgcaggcg atgcgggaag ccggcgagaa gatcgcgatg ctcaccagct acgacgcaag cttcgccgcc ctcctcgatc gcgcgaacgt cgacgtgcaa ctgatcggcg attcgctcgg caacgtgctg cagggccagg cgacgacgct gcccgtcacg ctcgacgaca tcgcgtatca caccgcatgc gtcgcgcgcg cgcagccgcg cgggctcgtc gtcgcggacc tgccgttcgg cacctacggc acgcccgccg acgcattcgc gagcgcggtg aagctgatgc gcgcgggcgc gcagatggtc aagctcgaag gcggcgaatg gctcgccgaa acggtgcgct tcctcgtcga gcgggccgtg cccgtgtgcg cgcacgtcgg cctcacgccg cagtcggtgc acgcgttcgg cggcttcaag gtgcaaggca agacggaagc aggcgccgcg caattgctgc gcgatgcgcg cgcggtcgag gaagccggcg cgcagctcat cgtgctcgag gcggtgccca cgctcgtcgc ggccgaagtc acgcgcgagc tgtcgatccc gacgatcggc atcggcgcgg gcgccgaatg ctcgggccag gtactcgtgc tgcacgacat gctcggcgtg ttccccggca agcggccgcg cttcgtcaag gatttcatgc aggggcagcc gagcatcttc gcagcggtcg aggcctacgt gcgcgcggtg aaggacggca gcttccccgg gcccgagcat tcgttctgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BupsA.00112.a.B1.GE25166
| HARVESTED ON: | 4/7/2008 |
| SEQUENCED ON: | 8/15/2008 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV TVPKLQAMRE AGEKIAMLTS YDASFAALLD RANVDVQLIG DSLGNVLQGQ ATTLPVTLDD IAYHTACVAR AQPRGLVVAD LPFGTYGTPA DAFASAVKLM RAGAQMVKLE GGEWLAETVR FLVERAVPVC AHVGLTPQSV HAFGGFKVQG KTEAGAAQLL RDARAVEEAG AQLIVLEAVP TLVAAEVTRE LSIPTIGIGA GAECSGQVLV LHDMLGVFPG KRPRFVKDFM QGQPSIFAAV EAYVRAVKDG SFPGPEHSF
Validated NT Sequence
atggctcacc accaccacca ccatatggtg acggtaccca agctgcaggc gatgcgggaa gccggcgaga agatcgcgat gctcaccagc tacgacgcaa gcttcgccgc cctcctcgat cgcgcgaacg tcgacgtgca actgatcggc gattcgctcg gcaacgtgct gcagggccag gcgacgacgc tgcccgtcac gctcgacgac atcgcgtatc acaccgcatg cgtcgcgcgc gcgcagccgc gcgggctcgt cgtcgcggac ctgccgttcg gcacctacgg cacgcccgcc gacgcattcg cgagcgcggt gaagctgatg cgcgcgggcg cgcagatggt caagctcgaa ggcggcgaat ggctcgccga aacggtgcgc ttcctcgtcg agcgggccgt gcccgtgtgc gcgcacgtcg gcctcacgcc gcagtcggtg cacgcgttcg gcggcttcaa ggtgcaaggc aagacggaag caggcgccgc gcaattgctg cgcgatgcgc gcgcggtcga ggaagccggc gcgcagctca tcgtgctcga ggcggtgccc acgctcgtcg cggccgaagt cacgcgcgag ctgtcgatcc cgacgatcgg catcggcgcg ggcgccgaat gctcgggcca ggtactcgtg ctgcacgaca tgctcggcgt gttccccggc aagcggccgc gcttcgtcaa ggatttcatg caggggcagc cgagcatctt cgcagcggtc gaggcctacg tgcgcgcggt gaaggacggc agcttccccg ggcccgagca ttcgttctaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMV TVPKLQAMRE AGEKIAMLTS YDASFAALLD RANVDVQLIG DSLGNVLQGQ ATTLPVTLDD IAYHTACVAR AQPRGLVVAD LPFGTYGTPA DAFASAVKLM RAGAQMVKLE GGEWLAETVR FLVERAVPVC AHVGLTPQSV HAFGGFKVQG KTEAGAAQLL RDARAVEEAG AQLIVLEAVP TLVAAEVTRE LSIPTIGIGA GAECSGQVLV LHDMLGVFPG KRPRFVKDFM QGQPSIFAAV EAYVRAVKDG SFPGPEHSF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga cggtacccaa gctgcaggcg atgcgggaag ccggcgagaa gatcgcgatg ctcaccagct acgacgcaag cttcgccgcc ctcctcgatc gcgcgaacgt cgacgtgcaa ctgatcggcg attcgctcgg caacgtgctg cagggccagg cgacgacgct gcccgtcacg ctcgacgaca tcgcgtatca caccgcatgc gtcgcgcgcg cgcagccgcg cgggctcgtc gtcgcggacc tgccgttcgg cacctacggc acgcccgccg acgcattcgc gagcgcggtg aagctgatgc gcgcgggcgc gcagatggtc aagctcgaag gcggcgaatg gctcgccgaa acggtgcgct tcctcgtcga gcgggccgtg cccgtgtgcg cgcacgtcgg cctcacgccg cagtcggtgc acgcgttcgg cggcttcaag gtgcaaggca agacggaagc aggcgccgcg caattgctgc gcgatgcgcg cgcggtcgag gaagccggcg cgcagctcat cgtgctcgag gcggtgccca cgctcgtcgc ggccgaagtc acgcgcgagc tgtcgatccc gacgatcggc atcggcgcgg gcgccgaatg ctcgggccag gtactcgtgc tgcacgacat gctcggcgtg ttccccggca agcggccgcg cttcgtcaag gatttcatgc aggggcagcc gagcatcttc gcagcggtcg aggcctacgt gcgcgcggtg aaggacggca gcttccccgg gcccgagcat tcgttctgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BupsA.00112.a.B1.PS00023
| PURIFICATION DATe: | 6/17/2008 |
| CONCENTRATION: | 24.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV TVPKLQAMRE AGEKIAMLTS YDASFAALLD RANVDVQLIG DSLGNVLQGQ ATTLPVTLDD IAYHTACVAR AQPRGLVVAD LPFGTYGTPA DAFASAVKLM RAGAQMVKLE GGEWLAETVR FLVERAVPVC AHVGLTPQSV HAFGGFKVQG KTEAGAAQLL RDARAVEEAG AQLIVLEAVP TLVAAEVTRE LSIPTIGIGA GAECSGQVLV LHDMLGVFPG KRPRFVKDFM QGQPSIFAAV EAYVRAVKDG SFPGPEHSF
Validated NT Sequence
atggctcacc accaccacca ccatatggtg acggtaccca agctgcaggc gatgcgggaa gccggcgaga agatcgcgat gctcaccagc tacgacgcaa gcttcgccgc cctcctcgat cgcgcgaacg tcgacgtgca actgatcggc gattcgctcg gcaacgtgct gcagggccag gcgacgacgc tgcccgtcac gctcgacgac atcgcgtatc acaccgcatg cgtcgcgcgc gcgcagccgc gcgggctcgt cgtcgcggac ctgccgttcg gcacctacgg cacgcccgcc gacgcattcg cgagcgcggt gaagctgatg cgcgcgggcg cgcagatggt caagctcgaa ggcggcgaat ggctcgccga aacggtgcgc ttcctcgtcg agcgggccgt gcccgtgtgc gcgcacgtcg gcctcacgcc gcagtcggtg cacgcgttcg gcggcttcaa ggtgcaaggc aagacggaag caggcgccgc gcaattgctg cgcgatgcgc gcgcggtcga ggaagccggc gcgcagctca tcgtgctcga ggcggtgccc acgctcgtcg cggccgaagt cacgcgcgag ctgtcgatcc cgacgatcgg catcggcgcg ggcgccgaat gctcgggcca ggtactcgtg ctgcacgaca tgctcggcgt gttccccggc aagcggccgc gcttcgtcaa ggatttcatg caggggcagc cgagcatctt cgcagcggtc gaggcctacg tgcgcgcggt gaaggacggc agcttccccg ggcccgagca ttcgttctaa gtgagtaaga t
Expressed Protein Sequence
MAHHHHHHMV TVPKLQAMRE AGEKIAMLTS YDASFAALLD RANVDVQLIG DSLGNVLQGQ ATTLPVTLDD IAYHTACVAR AQPRGLVVAD LPFGTYGTPA DAFASAVKLM RAGAQMVKLE GGEWLAETVR FLVERAVPVC AHVGLTPQSV HAFGGFKVQG KTEAGAAQLL RDARAVEEAG AQLIVLEAVP TLVAAEVTRE LSIPTIGIGA GAECSGQVLV LHDMLGVFPG KRPRFVKDFM QGQPSIFAAV EAYVRAVKDG SFPGPEHSF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTGA CGGTACCCAA GCTGCAGGCG ATGCGGGAAG CCGGCGAGAA GATCGCGATG CTCACCAGCT ACGACGCAAG CTTCGCCGCC CTCCTCGATC GCGCGAACGT CGACGTGCAA CTGATCGGCG ATTCGCTCGG CAACGTGCTG CAGGGCCAGG CGACGACGCT GCCCGTCACG CTCGACGACA TCGCGTATCA CACCGCATGC GTCGCGCGCG CGCAGCCGCG CGGGCTCGTC GTCGCGGACC TGCCGTTCGG CACCTACGGC ACGCCCGCCG ACGCATTCGC GAGCGCGGTG AAGCTGATGC GCGCGGGCGC GCAGATGGTC AAGCTCGAAG GCGGCGAATG GCTCGCCGAA ACGGTGCGCT TCCTCGTCGA GCGGGCCGTG CCCGTGTGCG CGCACGTCGG CCTCACGCCG CAGTCGGTGC ACGCGTTCGG CGGCTTCAAG GTGCAAGGCA AGACGGAAGC AGGCGCCGCG CAATTGCTGC GCGATGCGCG CGCGGTCGAG GAAGCCGGCG CGCAGCTCAT CGTGCTCGAG GCGGTGCCCA CGCTCGTCGC GGCCGAAGTC ACGCGCGAGC TGTCGATCCC GACGATCGGC ATCGGCGCGG GCGCCGAATG CTCGGGCCAG GTACTCGTGC TGCACGACAT GCTCGGCGTG TTCCCCGGCA AGCGGCCGCG CTTCGTCAAG GATTTCATGC AGGGGCAGCC GAGCATCTTC GCAGCGGTCG AGGCCTACGT GCGCGCGGTG AAGGACGGCA GCTTCCCCGG GCCCGAGCAT TCGTTCTGAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT