BupsA.00105.c

Beta-lactamase

CENTER ID: BupsA.00105.c
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00105.c.B1.GE41154 Full length( BupsA.00105.c ) 1 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1053
RefSeq: YP_332455.1
UniProt: Q3JVE8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLSSWSALG ERVDAAIDAA LAQRRLVGAV VLVARRGELA YRRAAGLADR EAGVPMREDA LFRFASVSKP IVSAAAMRAV AAGKLDLDAS IARWLPAFTP ALAGGRPARI TARQLLSHTA GLGYRFLETH AHGPYARAGV SDGMDCAGIS LAENLRRIAS VPLLYEPGTS WAYSLATDVL GALIEAVCDQ PLEDAVAEFV TTPLGMVDTR FYAHDAARLA AAYVDASDAA APGGPRRMAA LEIASPFPDT AGIRFEPARA LDAHAFASGG AGMVGTASDV LALIEALRTG GDGWLPAARI DEMARIQPGA EDLPTAPGYG FGLGFSVLRD PAAARSPESV GTWRWGGAYG HAWFVDRAAG LSVVALTNTA YEGMSGRFVA DLRDAVYGAG AAAQERAA
NT Sequence
ATGGTTCTTT CATCGTGGTC CGCGCTCGGC GAGCGGGTCG ACGCGGCGAT CGACGCCGCG CTCGCGCAGC GGCGCCTGGT CGGCGCGGTC GTGCTCGTCG CGCGCCGCGG CGAGCTCGCT TACCGGCGCG CGGCGGGGCT CGCCGATCGC GAGGCCGGCG TGCCGATGCG CGAGGACGCG CTGTTCCGGT TCGCGTCGGT ATCGAAGCCG ATCGTGTCGG CGGCGGCGAT GCGCGCTGTC GCGGCGGGCA AGCTCGATCT CGACGCGAGC ATCGCGCGCT GGCTGCCCGC GTTCACGCCC GCGCTGGCCG GCGGCCGCCC CGCGCGCATC ACCGCGCGGC AACTGCTGTC GCACACGGCG GGCCTCGGCT ACCGTTTTCT CGAAACGCAC GCGCACGGCC CGTATGCGCG CGCCGGCGTG TCCGACGGCA TGGATTGCGC GGGCATCTCG CTTGCCGAGA ACCTGCGCCG CATTGCGAGC GTGCCGCTGC TGTACGAGCC CGGCACGTCG TGGGCGTACT CGCTCGCGAC CGACGTGCTC GGCGCGCTGA TCGAGGCCGT ATGCGACCAG CCGCTCGAAG ACGCGGTGGC CGAGTTCGTG ACGACGCCGC TCGGCATGGT CGATACGCGT TTCTACGCGC ACGACGCGGC GCGGCTCGCG GCGGCGTACG TCGATGCGTC GGACGCCGCC GCGCCGGGCG GGCCGCGCCG CATGGCGGCG CTCGAGATCG CATCGCCGTT TCCCGACACG GCCGGCATTC GCTTCGAGCC CGCACGCGCG CTCGATGCGC ATGCGTTCGC GTCGGGCGGC GCCGGGATGG TCGGCACCGC GTCCGACGTG CTCGCGCTGA TCGAGGCGTT GCGCACCGGC GGCGACGGCT GGCTGCCCGC CGCGCGGATC GACGAGATGG CGCGCATCCA GCCGGGCGCG GAGGATCTGC CGACGGCGCC CGGCTACGGG TTCGGGCTCG GATTCTCGGT GCTGCGCGAT CCGGCCGCCG CGCGATCGCC GGAGTCCGTC GGCACGTGGC GCTGGGGCGG CGCGTATGGC CACGCGTGGT TCGTCGACCG CGCCGCCGGC TTGAGCGTCG TCGCACTCAC CAACACGGCT TACGAGGGGA TGTCGGGACG CTTCGTCGCC GACCTGCGCG ACGCCGTCTA CGGCGCGGGC GCGGCCGCGC AGGAGCGGGC GGCA
Details for BupsA.00105.c.B1.GE41154
HARVESTED ON: 3/1/2017
SEQUENCED ON: 3/2/2017
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMV LSSWSALGER VDAAIDAALA QRRLVGAVVL VARRGELAYR RAAGLADREA GVPMREDALF RFASVSKPIV SAAAMRAVAA GKLDLDASIA RWLPAFTPAL AGGRPARITA RQLLSHTAGL GYRFLETHAH GPYARAGVSD GMDCAGISLA ENLRRIASVP LLYEPGTSWA YSLATDVLGA LIEAVCDQPL EDAVAEFVTT PLGMVDTRFY AHDAARLAAA YVDASDAAAP GGPRRMAALE IASPFPDTAG IRFEPARALD AHAFASGGAG MVGTASDVLA LIEALRTGGD GWLPAARIDE MARIQPGAED LPTAPGYGFG LGFSVLRDPA AARSPESVGT WRWGGAYGHA WFVDRAAGLS VVALTNTAYE GMSGRFVADL RDAVYGAGAA AQERAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttc tttcatcgtg gtccgcgctc ggcgagcggg tcgacgcggc gatcgacgcc gcgctcgcgc agcggcgcct ggtcggcgcg gtcgtgctcg tcgcgcgccg cggcgagctc gcttaccggc gcgcggcggg gctcgccgat cgcgaggccg gcgtgccgat gcgcgaggac gcgctgttcc ggttcgcgtc ggtatcgaag ccgatcgtgt cggcggcggc gatgcgcgct gtcgcggcgg gcaagctcga tctcgacgcg agcatcgcgc gctggctgcc cgcgttcacg cccgcgctgg ccggcggccg ccccgcgcgc atcaccgcgc ggcaactgct gtcgcacacg gcgggcctcg gctaccgttt tctcgaaacg cacgcgcacg gcccgtatgc gcgcgccggc gtgtccgacg gcatggattg cgcgggcatc tcgcttgccg agaacctgcg ccgcattgcg agcgtgccgc tgctgtacga gcccggcacg tcgtgggcgt actcgctcgc gaccgacgtg ctcggcgcgc tgatcgaggc cgtatgcgac cagccgctcg aagacgcggt ggccgagttc gtgacgacgc cgctcggcat ggtcgatacg cgtttctacg cgcacgacgc ggcgcggctc gcggcggcgt acgtcgatgc gtcggacgcc gccgcgccgg gcgggccgcg ccgcatggcg gcgctcgaga tcgcatcgcc gtttcccgac acggccggca ttcgcttcga gcccgcacgc gcgctcgatg cgcatgcgtt cgcgtcgggc ggcgccggga tggtcggcac cgcgtccgac gtgctcgcgc tgatcgaggc gttgcgcacc ggcggcgacg gctggctgcc cgccgcgcgg atcgacgaga tggcgcgcat ccagccgggc gcggaggatc tgccgacggc gcccggctac gggttcgggc tcggattctc ggtgctgcgc gatccggccg ccgcgcgatc gccggagtcc gtcggcacgt ggcgctgggg cggcgcgtat ggccacgcgt ggttcgtcga ccgcgccgcc ggcttgagcg tcgtcgcact caccaacacg gcttacgagg ggatgtcggg acgcttcgtc gccgacctgc gcgacgccgt ctacggcgcg ggcgcggccg cgcaggagcg ggcggcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at