BupsA.00102.a

2-dehydro-3-deoxyphosphooctonate aldolase

CENTER ID: BupsA.00102.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00102.a.A1.GE28450 Full length( BupsA.00102.a ) 1 281
BupsA.00102.a.A5.GE27947 Created from Decode construct for feature BupsA.00102.a 1 275
BupsA.00102.a.A7.GE27949 Created from Decode construct for feature BupsA.00102.a 25 275
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Structures
3SZ8
DEPOSITED: 7/18/2011
DETERMINATION: XRay
CLONE: BupsA.00102.a.A1.GE28450
PROTEIN: BupsA.00102.a.A1.PS00621
3TMQ
DEPOSITED: 8/31/2011
DETERMINATION: XRay
CLONE: BupsA.00102.a.A1.GE28450
PROTEIN: BupsA.00102.a.A1.PS00621
3UND
DEPOSITED: 11/15/2011
DETERMINATION: XRay
CLONE: BupsA.00102.a.A1.GE28450
PROTEIN: BupsA.00102.a.A1.PS00621
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_130461
PATRIC ID: fig|320372.6.peg.3401
RefSeq: YP_334635
UniProt: Q3JP68
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVAISPGVT AGNSLPFVLF GGINVLESLD FTLDVCGEYV AVTRKLGIPF VFKASFDKAN RSSIHSYRGV GLDEGLKIFA EVKARFGVPV ITDVHEAEQA APVAEIADVL QVPAFLARQT DLVVAIAKAG KPVNVKKPQF MSPTQLKHVV SKCGEVGNDR VMLCERGSSF GYDNLVVDML GFRQMAETTG GCPVIFDVTH SLQCRDPLGD ASGGRRRQVL DLARAGIAVG IAGLFLEAHP DPDRARCDGP SALPLHQLEG LLSQMKAIDD LVKRMPALEI R
NT Sequence
ATGAACGTAG CAATCAGCCC CGGCGTCACG GCCGGCAACA GCCTGCCTTT CGTGCTGTTC GGCGGGATCA ACGTGCTCGA GAGTCTCGAC TTCACGCTCG ACGTGTGCGG CGAATACGTC GCGGTGACGC GCAAGCTCGG CATTCCGTTC GTGTTCAAGG CGTCGTTCGA CAAGGCGAAC CGCTCGTCGA TCCATTCGTA TCGCGGCGTC GGGCTCGACG AAGGCCTGAA GATCTTCGCC GAGGTGAAGG CGCGCTTCGG CGTGCCGGTG ATCACCGATG TGCACGAAGC CGAGCAGGCG GCGCCCGTGG CCGAAATCGC CGACGTGTTG CAGGTGCCCG CGTTTCTCGC GCGGCAGACC GATCTCGTCG TCGCGATCGC GAAGGCCGGC AAGCCGGTGA ACGTGAAGAA GCCGCAGTTC ATGAGCCCCA CGCAATTGAA GCACGTGGTG TCGAAATGCG GCGAGGTCGG CAACGATCGC GTGATGCTGT GCGAGCGCGG CAGTTCGTTC GGCTACGACA ATCTCGTCGT GGACATGCTC GGCTTCCGGC AGATGGCCGA GACGACGGGC GGTTGCCCGG TGATCTTCGA CGTCACGCAC AGCCTGCAGT GCCGCGATCC GCTCGGCGAC GCGTCGGGCG GCCGGCGCCG GCAAGTGCTC GATCTCGCGC GCGCGGGCAT CGCGGTCGGC ATCGCGGGGC TCTTTCTCGA GGCGCACCCC GATCCCGACC GCGCGCGCTG CGATGGGCCG AGCGCGCTGC CGTTGCATCA GCTCGAGGGC TTGCTGTCGC AGATGAAGGC GATCGACGAT CTCGTCAAGC GCATGCCGGC GCTCGAGATT CGA
Details for BupsA.00102.a.A1.GE28450
HARVESTED ON: 2/2/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNVAISPGV TAGNSLPFVL FGGINVLESL DFTLDVCGEY VAVTRKLGIP FVFKASFDKA NRSSIHSYRG VGLDEGLKIF AEVKARFGVP VITDVHEAEQ AAPVAEIADV LQVPAFLARQ TDLVVAIAKA GKPVNVKKPQ FMSPTQLKHV VSKCGEVGND RVMLCERGSS FGYDNLVVDM LGFRQMAETT GGCPVIFDVT HSLQCRDPLG DASGGRRRQV LDLARAGIAV GIAGLFLEAH PDPDRARCDG PSALPLHQLE GLLSQMKAID DLVKRMPALE IR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacg tagcaatcag ccccggcgtc acggccggca acagcctgcc tttcgtgctg ttcggcggga tcaacgtgct cgagagtctc gacttcacgc tcgacgtgtg cggcgaatac gtcgcggtga cgcgcaagct cggcattccg ttcgtgttca aggcgtcgtt cgacaaggcg aaccgctcgt cgatccattc gtatcgcggc gtcgggctcg acgaaggcct gaagatcttc gccgaggtga aggcgcgctt cggcgtgccg gtgatcaccg atgtgcacga agccgagcag gcggcgcccg tggccgaaat cgccgacgtg ttgcaggtgc ccgcgtttct cgcgcggcag accgatctcg tcgtcgcgat cgcgaaggcc ggcaagccgg tgaacgtgaa gaagccgcag ttcatgagcc ccacgcaatt gaagcacgtg gtgtcgaaat gcggcgaggt cggcaacgat cgcgtgatgc tgtgcgagcg cggcagttcg ttcggctacg acaatctcgt cgtggacatg ctcggcttcc ggcagatggc cgagacgacg ggcggttgcc cggtgatctt cgacgtcacg cacagcctgc agtgccgcga tccgctcggc gacgcgtcgg gcggccggcg ccggcaagtg ctcgatctcg cgcgcgcggg catcgcggtc ggcatcgcgg ggctctttct cgaggcgcac cccgatcccg accgcgcgcg ctgcgatggg ccgagcgcgc tgccgttgca tcagctcgag ggcttgctgt cgcagatgaa ggcgatcgac gatctcgtca agcgcatgcc ggcgctcgag attcgataat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNVAISPGV TAGNSLPFVL FGGINVLESL DFTLDVCGEY VAVTRKLGIP FVFKASFDKA NRSSIHSYRG VGLDEGLKIF AEVKARFGVP VITDVHEAEQ AAPVAEIADV LQVPAFLARQ TDLVVAIAKA GKPVNVKKPQ FMSPTQLKHV VSKCGEVGND RVMLCERGSS FGYDNLVVDM LGFRQMAETT GGCPVIFDVT HSLQCRDPLG DASGGRRRQV LDLARAGIAV GIAGLFLEAH PDPDRARCDG PSALPLHQLE GLLSQMKAID DLVKRMPALE IR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgtagca atcagccccg gcgtcacggc cggcaacagc ctgcctttcg tgctgttcgg cgggatcaac gtgctcgaga gtctcgactt cacgctcgac gtgtgcggcg aatacgtcgc ggtgacgcgc aagctcggca ttccgttcgt gttcaaggcg tcgttcgaca aggcgaaccg ctcgtcgatc cattcgtatc gcggcgtcgg gctcgacgaa ggcctgaaga tcttcgccga ggtgaaggcg cgcttcggcg tgccggtgat caccgatgtg cacgaagccg agcaggcggc gcccgtggcc gaaatcgccg acgtgttgca ggtgcccgcg tttctcgcgc ggcagaccga tctcgtcgtc gcgatcgcga aggccggcaa gccggtgaac gtgaagaagc cgcagttcat gagccccacg caattgaagc acgtggtgtc gaaatgcggc gaggtcggca acgatcgcgt gatgctgtgc gagcgcggca gttcgttcgg ctacgacaat ctcgtcgtgg acatgctcgg cttccggcag atggccgaga cgacgggcgg ttgcccggtg atcttcgacg tcacgcacag cctgcagtgc cgcgatccgc tcggcgacgc gtcgggcggc cggcgccggc aagtgctcga tctcgcgcgc gcgggcatcg cggtcggcat cgcggggctc tttctcgagg cgcaccccga tcccgaccgc gcgcgctgcg atgggccgag cgcgctgccg ttgcatcagc tcgagggctt gctgtcgcag atgaaggcga tcgacgatct cgtcaagcgc atgccggcgc tcgagattcg atgaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BupsA.00102.a.A5.GE27947
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNVAISPGV TAGNSLPFVL FGGINVLESL DFTLDVCGEY VAVTRKLGIP FVFKASFDKA NRSSIHSYRG VGLDEGLKIF AEVKARFGVP VITDVHEAEQ AAPVAEIADV LQVPAFLARQ TDLVVAIAKA GKPVNVKKPQ FMSPTQLKHV VSKCGEVGND RVMLCERGSS FGYDNLVVDM LGFRQMAETT GGCPVIFDVT HSLQCRDPLG DASGGRRRQV LDLARAGIAV GIAGLFLEAH PDPDRARCDG PSALPLHQLE GLLSQMKAID DLVKRM
Validated NT Sequence
ttattacatg cgcttgacga gatcgtcgat cgccttcatc tgcgacagca agccctcgag ctgatgcaac ggcagcgcgc tcggcccatc gcagcgcgcg cggtcgggat cggggtgcgc ctcgagaaag agccccgcga tgccgaccgc gatgcccgcg cgcgcgagat cgagcacttg ccggcgccgg ccgcccgacg cgtcgccgag cggatcgcgg cactgcaggc tgtgcgtgac gtcgaagatc accgggcaac cgcccgtcgt ctcggccatc tgccggaagc cgagcatgtc cacgacgaga ttgtcgtagc cgaacgaact gccgcgctcg cacagcatca cgcgatcgtt gccgacctcg ccgcatttcg acaccacgtg cttcaattgc gtggggctca tgaactgcgg cttcttcacg ttcaccggct tgccggcctt cgcgatcgcg acgacgagat cggtctgccg cgcgagaaac gcgggcacct gcaacacgtc ggcgatttcg gccacgggcg ccgcctgctc ggcttcgtgc acatcggtga tcaccggcac gccgaagcgc gccttcacct cggcgaagat cttcaggcct tcgtcgagcc cgacgccgcg atacgaatgg atcgacgagc ggttcgcctt gtcgaacgac gccttgaaca cgaacggaat gccgagcttg cgcgtcaccg cgacgtattc gccgcacacg tcgagcgtga agtcgagact ctcgagcacg ttgatcccgc cgaacagcac gaaaggcagg ctgttgccgg ccgtgacgcc ggggctgatt gctacgttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNVAISPGV TAGNSLPFVL FGGINVLESL DFTLDVCGEY VAVTRKLGIP FVFKASFDKA NRSSIHSYRG VGLDEGLKIF AEVKARFGVP VITDVHEAEQ AAPVAEIADV LQVPAFLARQ TDLVVAIAKA GKPVNVKKPQ FMSPTQLKHV VSKCGEVGND RVMLCERGSS FGYDNLVVDM LGFRQMAETT GGCPVIFDVT HSLQCRDPLG DASGGRRRQV LDLARAGIAV GIAGLFLEAH PDPDRARCDG PSALPLHQLE GLLSQMKAID DLVKRM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgtagca atcagccccg gcgtcacggc cggcaacagc ctgcctttcg tgctgttcgg cgggatcaac gtgctcgaga gtctcgactt cacgctcgac gtgtgcggcg aatacgtcgc ggtgacgcgc aagctcggca ttccgttcgt gttcaaggcg tcgttcgaca aggcgaaccg ctcgtcgatc cattcgtatc gcggcgtcgg gctcgacgaa ggcctgaaga tcttcgccga ggtgaaggcg cgcttcggcg tgccggtgat caccgatgtg cacgaagccg agcaggcggc gcccgtggcc gaaatcgccg acgtgttgca ggtgcccgcg tttctcgcgc ggcagaccga tctcgtcgtc gcgatcgcga aggccggcaa gccggtgaac gtgaagaagc cgcagttcat gagccccacg caattgaagc acgtggtgtc gaaatgcggc gaggtcggca acgatcgcgt gatgctgtgc gagcgcggca gttcgttcgg ctacgacaat ctcgtcgtgg acatgctcgg cttccggcag atggccgaga cgacgggcgg ttgcccggtg atcttcgacg tcacgcacag cctgcagtgc cgcgatccgc tcggcgacgc gtcgggcggc cggcgccggc aagtgctcga tctcgcgcgc gcgggcatcg cggtcggcat cgcggggctc tttctcgagg cgcaccccga tcccgaccgc gcgcgctgcg atgggccgag cgcgctgccg ttgcatcagc tcgagggctt gctgtcgcag atgaaggcga tcgacgatct cgtcaagcgc atgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BupsA.00102.a.A7.GE27949
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLESLDFT LDVCGEYVAV TRKLGIPFVF KASFDKANRS SIHSYRGVGL DEGLKIFAEV KARFGVPVIT DVHEAEQAAP VAEIADVLQV PAFLARQTDL VVAIAKAGKP VNVKKPQFMS PTQLKHVVSK CGEVGNDRVM LCERGSSFGY DNLVVDMLGF RQMAETTGGC PVIFDVTHSL QCRDPLGDAS GGRRRQVLDL ARAGIAVGIA GLFLEAHPDP DRARCDGPSA LPLHQLEGLL SQMKAIDDLV KRM
Validated NT Sequence
ttattacatg cgcttgacga gatcgtcgat cgccttcatc tgcgacagca agccctcgag ctgatgcaac ggcagcgcgc tcggcccatc gcagcgcgcg cggtcgggat cggggtgcgc ctcgagaaag agccccgcga tgccgaccgc gatgcccgcg cgcgcgagat cgagcacttg ccggcgccgg ccgcccgacg cgtcgccgag cggatcgcgg cactgcaggc tgtgcgtgac gtcgaagatc accgggcaac cgcccgtcgt ctcggccatc tgccggaagc cgagcatgtc cacgacgaga ttgtcgtagc cgaacgaact gccgcgctcg cacagcatca cgcgatcgtt gccgacctcg ccgcatttcg acaccacgtg cttcaattgc gtggggctca tgaactgcgg cttcttcacg ttcaccggct tgccggcctt cgcgatcgcg acgacgagat cggtctgccg cgcgagaaac gcgggcacct gcaacacgtc ggcgatttcg gccacgggcg ccgcctgctc ggcttcgtgc acatcggtga tcaccggcac gccgaagcgc gccttcacct cggcgaagat cttcaggcct tcgtcgagcc cgacgccgcg atacgaatgg atcgacgagc ggttcgcctt gtcgaacgac gccttgaaca cgaacggaat gccgagcttg cgcgtcaccg cgacgtattc gccgcacacg tcgagcgtga agtcgagact ctcgagcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLESLDFT LDVCGEYVAV TRKLGIPFVF KASFDKANRS SIHSYRGVGL DEGLKIFAEV KARFGVPVIT DVHEAEQAAP VAEIADVLQV PAFLARQTDL VVAIAKAGKP VNVKKPQFMS PTQLKHVVSK CGEVGNDRVM LCERGSSFGY DNLVVDMLGF RQMAETTGGC PVIFDVTHSL QCRDPLGDAS GGRRRQVLDL ARAGIAVGIA GLFLEAHPDP DRARCDGPSA LPLHQLEGLL SQMKAIDDLV KRM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctcgag agtctcgact tcacgctcga cgtgtgcggc gaatacgtcg cggtgacgcg caagctcggc attccgttcg tgttcaaggc gtcgttcgac aaggcgaacc gctcgtcgat ccattcgtat cgcggcgtcg ggctcgacga aggcctgaag atcttcgccg aggtgaaggc gcgcttcggc gtgccggtga tcaccgatgt gcacgaagcc gagcaggcgg cgcccgtggc cgaaatcgcc gacgtgttgc aggtgcccgc gtttctcgcg cggcagaccg atctcgtcgt cgcgatcgcg aaggccggca agccggtgaa cgtgaagaag ccgcagttca tgagccccac gcaattgaag cacgtggtgt cgaaatgcgg cgaggtcggc aacgatcgcg tgatgctgtg cgagcgcggc agttcgttcg gctacgacaa tctcgtcgtg gacatgctcg gcttccggca gatggccgag acgacgggcg gttgcccggt gatcttcgac gtcacgcaca gcctgcagtg ccgcgatccg ctcggcgacg cgtcgggcgg ccggcgccgg caagtgctcg atctcgcgcg cgcgggcatc gcggtcggca tcgcggggct ctttctcgag gcgcaccccg atcccgaccg cgcgcgctgc gatgggccga gcgcgctgcc gttgcatcag ctcgagggct tgctgtcgca gatgaaggcg atcgacgatc tcgtcaagcg catgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa