BupsA.00094.a

acetylglutamate kinase

CENTER ID:	BupsA.00094.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00094.a.B1.GE25152	Full length( BupsA.00094.a )		1	351

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00094.a.B1.PW25190	Full length( BupsA.00094.a )		1	351

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130618
PATRIC ID:	fig\|320372.6.peg.383
RefSeq:	YP_331794
UniProt:	Q3JXA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRIAQENLTF PAAAPDFDTP AAHRPNGQKP HAALRHSCTI PPVSTFRTRA AAMSEPIDLS QISPALKAEI LAEALPYIRR YHGKTVVIKY GGNAMTEERL KQGFARDVIL LKLVGINPVI VHGGGPQIDQ ALKKIGKQGT FIQGMRVTDE ETMEVVEWVL GGEVQQDIVT LINHFGGHAV GLTGKDGGLI HARKLMMPDR DNPGEYVDIG QVGEVEAINP AVVKALQDDA FIPVISPIGF GEDGLSYNIN ADLVAGKLAT VLNAEKLVMM TNIPGVMDKE GNLLTDLSAR EIDALFEDGT ISGGMLPKIS SALDAAKSGV KSVHIVDGRI EHSVLLEILT EQPFGTMIRS H

NT Sequence

ATGCGGATCG CGCAGGAAAA CCTCACTTTC CCGGCGGCGG CGCCCGATTT CGACACGCCC GCCGCCCATC GTCCGAACGG CCAAAAACCC CATGCTGCGC TGCGCCACTC CTGTACAATT CCGCCTGTTT CAACGTTTCG CACCCGAGCC GCCGCCATGT CCGAGCCGAT CGACCTCTCG CAGATCTCCC CCGCCCTGAA AGCCGAAATC CTTGCGGAGG CGCTGCCGTA CATTCGCCGC TACCACGGCA AGACCGTGGT GATCAAATAC GGCGGCAATG CGATGACGGA AGAACGGCTC AAGCAGGGCT TCGCGCGCGA CGTGATCCTG CTGAAGCTCG TCGGCATCAA CCCGGTGATC GTGCACGGCG GCGGCCCGCA GATCGACCAG GCGCTCAAGA AGATCGGCAA GCAGGGCACG TTCATTCAGG GAATGCGCGT CACCGACGAA GAGACGATGG AAGTCGTCGA ATGGGTGCTG GGCGGCGAGG TGCAGCAGGA CATCGTCACG CTGATCAACC ATTTCGGCGG CCACGCGGTC GGCCTGACGG GCAAGGACGG CGGCCTCATC CACGCGCGCA AGCTGATGAT GCCGGACCGC GACAACCCGG GCGAGTACGT CGACATCGGC CAGGTCGGCG AGGTCGAGGC GATCAATCCG GCCGTCGTGA AGGCGCTGCA GGACGATGCG TTCATCCCGG TGATCTCGCC GATCGGCTTC GGCGAGGACG GCCTGTCGTA CAACATCAAC GCGGATCTCG TCGCGGGCAA GCTCGCGACC GTGCTGAACG CCGAGAAGCT CGTGATGATG ACCAACATCC CCGGCGTGAT GGACAAGGAA GGCAACCTGC TGACCGACCT GTCCGCGCGC GAGATCGACG CGCTCTTCGA GGACGGCACG ATCTCGGGCG GCATGCTGCC GAAAATCTCG TCCGCGCTCG ACGCGGCGAA GAGCGGCGTG AAGTCGGTCC ATATCGTCGA CGGCCGCATC GAGCATTCGG TGCTGCTCGA AATCCTGACC GAGCAGCCGT TCGGCACGAT GATCCGCTCG CAT

Details for BupsA.00094.a.B1.GE25152

HARVESTED ON:	2/26/2008
SEQUENCED ON:	8/15/2008
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR IAQENLTFPA AAPDFDTPAA HRPNGQKPHA ALRHSCTIPP VSTFRTRAAA MSEPIDLSQI SPALKAEILA EALPYIRRYH GKTVVIKYGG NAMTEERLKQ GFARDVILLK LVGINPVIVH GGGPQIDQAL KKIGKQGTFI QGMRVTDEET MEVVEWVLGG EVQQDIVTLI NHFGGHAVGL TGKDGGLIHA RKLMMPDRDN PGEYVDIGQV GEVEAINPAV VKALQDDAFI PVISPIGFGE DSLSYNINAD LVAGKLATVL NAEKLVMMTN IPGVMDKEGN LLTDLSAREI DALFEDGTIS GGMLPKISSA LDAAKSGVKS VHIVDGRIEH SVLLEILTEQ PFGTMIRSH

Validated NT Sequence

atggctcacc accaccacca ccatatgcgg atcgcgcagg aaaacctcac tttcccggcg gcggcgcccg atttcgacac gcccgccgcc catcgtccga acggccaaaa accccatgct gcgctgcgcc actcctgtac aattccgcct gtttcaacgt ttcgcacccg agccgccgcc atgtccgagc cgatcgacct ctcgcagatc tcccccgccc tgaaagccga aatccttgcg gaggcgctgc cgtacattcg ccgctaccac ggcaagaccg tggtgatcaa atacggcggc aatgcgatga cggaagaacg gctcaagcag ggcttcgcgc gcgacgtgat cctgctgaag ctcgtcggca tcaacccggt gatcgtgcac ggcggcggcc cgcagatcga ccaggcgctc aagaagatcg gcaagcaggg cacgttcatt cagggaatgc gcgtcaccga cgaagagacg atggaagtcg tcgaatgggt gctgggcggc gaggtgcagc aggacatcgt cacgctgatc aaccatttcg gcggccacgc ggtcggcctg acgggcaagg acggcggcct catccacgcg cgcaagctga tgatgccgga ccgcgacaac ccgggcgagt acgtcgacat cggccaggtc ggcgaggtcg aggcgatcaa tccggccgtc gtgaaggcgc tgcaggacga tgcgttcatc ccggtgatct cgccgatcgg cttcggcgag gacagcctgt cgtacaacat caacgcggat ctcgtcgcgg gcaagctcgc gaccgtgctg aacgccgaga agctcgtgat gatgaccaac atccccggcg tgatggacaa ggaaggcaac ctgctgaccg acctgtccgc gcgcgagatc gacgcgctct tcgaggacgg cacgatctcg ggcggcatgc tgccgaaaat ctcgtccgcg ctcgacgcgg cgaagagcgg cgtgaagtcg gtccatatcg tcgacggccg catcgagcat tcggtgctgc tcgaaatcct gaccgagcag ccgttcggca cgatgatccg ctcgcatt

Expected Protein Sequence

MAHHHHHHMR IAQENLTFPA AAPDFDTPAA HRPNGQKPHA ALRHSCTIPP VSTFRTRAAA MSEPIDLSQI SPALKAEILA EALPYIRRYH GKTVVIKYGG NAMTEERLKQ GFARDVILLK LVGINPVIVH GGGPQIDQAL KKIGKQGTFI QGMRVTDEET MEVVEWVLGG EVQQDIVTLI NHFGGHAVGL TGKDGGLIHA RKLMMPDRDN PGEYVDIGQV GEVEAINPAV VKALQDDAFI PVISPIGFGE DGLSYNINAD LVAGKLATVL NAEKLVMMTN IPGVMDKEGN LLTDLSAREI DALFEDGTIS GGMLPKISSA LDAAKSGVKS VHIVDGRIEH SVLLEILTEQ PFGTMIRSH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgga tcgcgcagga aaacctcact ttcccggcgg cggcgcccga tttcgacacg cccgccgccc atcgtccgaa cggccaaaaa ccccatgctg cgctgcgcca ctcctgtaca attccgcctg tttcaacgtt tcgcacccga gccgccgcca tgtccgagcc gatcgacctc tcgcagatct cccccgccct gaaagccgaa atccttgcgg aggcgctgcc gtacattcgc cgctaccacg gcaagaccgt ggtgatcaaa tacggcggca atgcgatgac ggaagaacgg ctcaagcagg gcttcgcgcg cgacgtgatc ctgctgaagc tcgtcggcat caacccggtg atcgtgcacg gcggcggccc gcagatcgac caggcgctca agaagatcgg caagcagggc acgttcattc agggaatgcg cgtcaccgac gaagagacga tggaagtcgt cgaatgggtg ctgggcggcg aggtgcagca ggacatcgtc acgctgatca accatttcgg cggccacgcg gtcggcctga cgggcaagga cggcggcctc atccacgcgc gcaagctgat gatgccggac cgcgacaacc cgggcgagta cgtcgacatc ggccaggtcg gcgaggtcga ggcgatcaat ccggccgtcg tgaaggcgct gcaggacgat gcgttcatcc cggtgatctc gccgatcggc ttcggcgagg acggcctgtc gtacaacatc aacgcggatc tcgtcgcggg caagctcgcg accgtgctga acgccgagaa gctcgtgatg atgaccaaca tccccggcgt gatggacaag gaaggcaacc tgctgaccga cctgtccgcg cgcgagatcg acgcgctctt cgaggacggc acgatctcgg gcggcatgct gccgaaaatc tcgtccgcgc tcgacgcggc gaagagcggc gtgaagtcgg tccatatcgt cgacggccgc atcgagcatt cggtgctgct cgaaatcctg accgagcagc cgttcggcac gatgatccgc tcgcattgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BupsA.00094.a.B1.PW25190

PURIFICATION DATe:	4/7/2008
CONCENTRATION:	24.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	50µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMR IAQENLTFPA AAPDFDTPAA HRPNGQKPHA ALRHSCTIPP VSTFRTRAAA MSEPIDLSQI SPALKAEILA EALPYIRRYH GKTVVIKYGG NAMTEERLKQ GFARDVILLK LVGINPVIVH GGGPQIDQAL KKIGKQGTFI QGMRVTDEET MEVVEWVLGG EVQQDIVTLI NHFGGHAVGL TGKDGGLIHA RKLMMPDRDN PGEYVDIGQV GEVEAINPAV VKALQDDAFI PVISPIGFGE DGLSYNINAD LVAGKLATVL NAEKLVMMTN IPGVMDKEGN LLTDLSAREI DALFEDGTIS GGMLPKISSA LDAAKSGVKS VHIVDGRIEH SVLLEILTEQ PFGTMIRSH

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGGA TCGCGCAGGA AAACCTCACT TTCCCGGCGG CGGCGCCCGA TTTCGACACG CCCGCCGCCC ATCGTCCGAA CGGCCAAAAA CCCCATGCTG CGCTGCGCCA CTCCTGTACA ATTCCGCCTG TTTCAACGTT TCGCACCCGA GCCGCCGCCA TGTCCGAGCC GATCGACCTC TCGCAGATCT CCCCCGCCCT GAAAGCCGAA ATCCTTGCGG AGGCGCTGCC GTACATTCGC CGCTACCACG GCAAGACCGT GGTGATCAAA TACGGCGGCA ATGCGATGAC GGAAGAACGG CTCAAGCAGG GCTTCGCGCG CGACGTGATC CTGCTGAAGC TCGTCGGCAT CAACCCGGTG ATCGTGCACG GCGGCGGCCC GCAGATCGAC CAGGCGCTCA AGAAGATCGG CAAGCAGGGC ACGTTCATTC AGGGAATGCG CGTCACCGAC GAAGAGACGA TGGAAGTCGT CGAATGGGTG CTGGGCGGCG AGGTGCAGCA GGACATCGTC ACGCTGATCA ACCATTTCGG CGGCCACGCG GTCGGCCTGA CGGGCAAGGA CGGCGGCCTC ATCCACGCGC GCAAGCTGAT GATGCCGGAC CGCGACAACC CGGGCGAGTA CGTCGACATC GGCCAGGTCG GCGAGGTCGA GGCGATCAAT CCGGCCGTCG TGAAGGCGCT GCAGGACGAT GCGTTCATCC CGGTGATCTC GCCGATCGGC TTCGGCGAGG ACGGCCTGTC GTACAACATC AACGCGGATC TCGTCGCGGG CAAGCTCGCG ACCGTGCTGA ACGCCGAGAA GCTCGTGATG ATGACCAACA TCCCCGGCGT GATGGACAAG GAAGGCAACC TGCTGACCGA CCTGTCCGCG CGCGAGATCG ACGCGCTCTT CGAGGACGGC ACGATCTCGG GCGGCATGCT GCCGAAAATC TCGTCCGCGC TCGACGCGGC GAAGAGCGGC GTGAAGTCGG TCCATATCGT CGACGGCCGC ATCGAGCATT CGGTGCTGCT CGAAATCCTG ACCGAGCAGC CGTTCGGCAC GATGATCCGC TCGCATTGAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT