BupsA.00093.a
N-ethylmaleimide reductase
| CENTER ID: | BupsA.00093.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00093.a.A1.GE25055 | Full length( BupsA.00093.a ) | 1 | 353 | ||
| BupsA.00093.a.A1.GE25055 | Full length( BupsA.00093.a ) | 1 | 353 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00093.a.A1.PS00075 | Full length( BupsA.00093.a ) | 1 | 353 | ||
| BupsA.00093.a.B1.PS00139 | Full length( BupsA.00093.a ) | 1 | 353 | ||
| BupsA.00093.a.A1.PW25093 | Full length( BupsA.00093.a ) | 1 | 353 |
Structures
3GKA
DEPOSITED: 3/10/2009
DETERMINATION: XRay
CLONE: BupsA.00093.a.A1.GE25055
PROTEIN: BupsA.00093.a.A1.PS00075
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|320372.6.peg.6682 |
| RefSeq: | YP_337624 |
| UniProt: | Q3JFM9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSLFDPLTI GDLTLANRII MAPLTRARAG DTRTPNALMA RYYAERASAG LIISEATSVT PQGVGYASTP GIWSPEQVDG WRLVTDAVHA AGGRIFLQLW HVGRVSDPVF LDGALPVAPS AIAPGGHVSL VRPQRPYVTP RALELDEIPG VVAAFRRGAE NARAAGFDGV EVHGANGYLL DQFLQDSANR RTDAYGGSIE NRARLLLEVV DAAIDVWSAA RVGVHLAPRG DAHTMGDSDP AATFGHVARE LGRRRIAFLF ARESFGGDAI GQQLKAAFGG PFIVNENFTL DSAQAALDAG QADAVAWGKL FIANPDLPRR FKLNAPLNEP NAATFYAQGE VGYTDYPALE SAA
NT Sequence
ATGCCTTCTC TGTTCGATCC GCTGACCATC GGCGACCTCA CACTGGCGAA CCGCATCATC ATGGCGCCGC TCACGCGCGC CCGCGCCGGC GACACGCGCA CGCCCAACGC GCTGATGGCG CGCTACTACG CGGAGCGCGC ATCCGCGGGG CTCATCATCA GCGAGGCGAC ATCGGTCACG CCGCAAGGCG TCGGTTACGC AAGCACGCCG GGCATCTGGT CGCCCGAACA GGTCGACGGG TGGCGACTCG TGACGGACGC GGTCCACGCG GCCGGCGGCC GCATCTTCCT GCAGCTCTGG CACGTCGGCC GCGTGTCGGA TCCGGTGTTC CTCGACGGCG CGCTGCCCGT CGCGCCGAGC GCGATCGCCC CCGGCGGCCA CGTGAGCCTC GTGCGCCCGC AGCGGCCGTA CGTGACGCCG CGCGCGCTCG AGCTCGACGA GATTCCGGGC GTCGTCGCCG CGTTCCGCCG CGGCGCGGAA AACGCGCGCG CGGCCGGCTT CGACGGCGTC GAGGTACACG GCGCGAACGG CTATCTGCTC GATCAATTCC TGCAGGACAG CGCGAACCGC CGCACCGACG CGTACGGCGG CTCGATCGAG AACCGCGCGC GCCTGTTGCT CGAGGTGGTC GACGCGGCGA TCGACGTCTG GAGCGCGGCG CGCGTCGGCG TGCATCTCGC GCCGCGCGGC GATGCGCACA CGATGGGCGA TTCGGACCCC GCGGCTACGT TCGGCCACGT CGCGCGCGAA CTCGGCCGCC GCCGCATCGC GTTCCTGTTC GCACGCGAAT CGTTCGGCGG CGACGCCATC GGCCAGCAAT TGAAGGCGGC GTTCGGCGGG CCATTCATCG TGAACGAGAA TTTCACGCTC GACAGCGCGC AGGCGGCGCT CGACGCGGGA CAGGCGGACG CGGTCGCGTG GGGCAAGCTT TTTATCGCGA ATCCGGACCT GCCGCGCCGC TTCAAGCTGA ATGCGCCGCT CAACGAGCCG AATGCCGCTA CGTTCTATGC GCAAGGCGAA GTAGGCTATA CGGACTATCC GGCGCTGGAA AGCGCGGCG
Details for BupsA.00093.a.A1.GE25055
| HARVESTED ON: | 3/31/2008 |
| SEQUENCED ON: | 7/29/2008 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | failed alignment |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSLFDPLT IGDLTLANRI IMAPLTRARA GDTRTPNALM ARYYAERASA GLIISEATSV TPQGVGYAST PGIWSPEQVD GWRLVTDAVH AAGGRIFLQL WHVGRVSDPV FLDGALPVAP SAIAPGGHVS LVRPQRPYVT PRALELDEIP GVVAAFRRGA ENARAAGFDG VEVHGANGYL LDQFLQDSAN RRTDAYGGSI ENRARLLLEV VDAAIDVWSA ARVGVHLAPR GDAHTMGDSD PAATFGHVAR ELGRRRIAFL FARESFGGDA IGQQLKAAFG GPFIVNENFT LDSAQAALDA GQADAVAWGK LFIANPDLPR RFKLNAPLNE PNAATFYAQG EVGYTDYPAL ESAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctt ctctgttcga tccgctgacc atcggcgacc tcacactggc gaaccgcatc atcatggcgc cgctcacgcg cgcccgcgcc ggcgacacgc gcacgcccaa cgcgctgatg gcgcgctact acgcggagcg cgcatccgcg gggctcatca tcagcgaggc gacatcggtc acgccgcaag gcgtcggtta cgcaagcacg ccgggcatct ggtcgcccga acaggtcgac gggtggcgac tcgtgacgga cgcggtccac gcggccggcg gccgcatctt cctgcagctc tggcacgtcg gccgcgtgtc ggatccggtg ttcctcgacg gcgcgctgcc cgtcgcgccg agcgcgatcg cccccggcgg ccacgtgagc ctcgtgcgcc cgcagcggcc gtacgtgacg ccgcgcgcgc tcgagctcga cgagattccg ggcgtcgtcg ccgcgttccg ccgcggcgcg gaaaacgcgc gcgcggccgg cttcgacggc gtcgaggtac acggcgcgaa cggctatctg ctcgatcaat tcctgcagga cagcgcgaac cgccgcaccg acgcgtacgg cggctcgatc gagaaccgcg cgcgcctgtt gctcgaggtg gtcgacgcgg cgatcgacgt ctggagcgcg gcgcgcgtcg gcgtgcatct cgcgccgcgc ggcgatgcgc acacgatggg cgattcggac cccgcggcta cgttcggcca cgtcgcgcgc gaactcggcc gccgccgcat cgcgttcctg ttcgcacgcg aatcgttcgg cggcgacgcc atcggccagc aattgaaggc ggcgttcggc gggccattca tcgtgaacga gaatttcacg ctcgacagcg cgcaggcggc gctcgacgcg ggacaggcgg acgcggtcgc gtggggcaag ctttttatcg cgaatccgga cctgccgcgc cgcttcaagc tgaatgcgcc gctcaacgag ccgaatgccg ctacgttcta tgcgcaaggc gaagtaggct atacggacta tccggcgctg gaaagcgcgg cgtaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSLFDPLT IGDLTLANRI IMAPLTRARA GDTRTPNALM ARYYAERASA GLIISEATSV TPQGVGYAST PGIWSPEQVD GWRLVTDAVH AAGGRIFLQL WHVGRVSDPV FLDGALPVAP SAIAPGGHVS LVRPQRPYVT PRALELDEIP GVVAAFRRGA ENARAAGFDG VEVHGANGYL LDQFLQDSAN RRTDAYGGSI ENRARLLLEV VDAAIDVWSA ARVGVHLAPR GDAHTMGDSD PAATFGHVAR ELGRRRIAFL FARESFGGDA IGQQLKAAFG GPFIVNENFT LDSAQAALDA GQADAVAWGK LFIANPDLPR RFKLNAPLNE PNAATFYAQG EVGYTDYPAL ESAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccttctctg ttcgatccgc tgaccatcgg cgacctcaca ctggcgaacc gcatcatcat ggcgccgctc acgcgcgccc gcgccggcga cacgcgcacg cccaacgcgc tgatggcgcg ctactacgcg gagcgcgcat ccgcggggct catcatcagc gaggcgacat cggtcacgcc gcaaggcgtc ggttacgcaa gcacgccggg catctggtcg cccgaacagg tcgacgggtg gcgactcgtg acggacgcgg tccacgcggc cggcggccgc atcttcctgc agctctggca cgtcggccgc gtgtcggatc cggtgttcct cgacggcgcg ctgcccgtcg cgccgagcgc gatcgccccc ggcggccacg tgagcctcgt gcgcccgcag cggccgtacg tgacgccgcg cgcgctcgag ctcgacgaga ttccgggcgt cgtcgccgcg ttccgccgcg gcgcggaaaa cgcgcgcgcg gccggcttcg acggcgtcga ggtacacggc gcgaacggct atctgctcga tcaattcctg caggacagcg cgaaccgccg caccgacgcg tacggcggct cgatcgagaa ccgcgcgcgc ctgttgctcg aggtggtcga cgcggcgatc gacgtctgga gcgcggcgcg cgtcggcgtg catctcgcgc cgcgcggcga tgcgcacacg atgggcgatt cggaccccgc ggctacgttc ggccacgtcg cgcgcgaact cggccgccgc cgcatcgcgt tcctgttcgc acgcgaatcg ttcggcggcg acgccatcgg ccagcaattg aaggcggcgt tcggcgggcc attcatcgtg aacgagaatt tcacgctcga cagcgcgcag gcggcgctcg acgcgggaca ggcggacgcg gtcgcgtggg gcaagctttt tatcgcgaat ccggacctgc cgcgccgctt caagctgaat gcgccgctca acgagccgaa tgccgctacg ttctatgcgc aaggcgaagt aggctatacg gactatccgg cgctggaaag cgcggcgtga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.00093.a.A1.GE25055
| HARVESTED ON: | 3/31/2008 |
| SEQUENCED ON: | 7/29/2008 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSLFDPLT IGDLTLANRI IMAPLTRARA GDTRTPNALM ARYYAERASA GLIISEATSV TPQGVGYAST PGIWSPEQVD GWRLVTDAVH AAGGRIFLQL WHVGRVSDPV FLDGALPVAP SAIAPGGHVS LVRPQRPYVT PRALELDEIP GVVAAFRRGA ENARAAGFDG VEVHGANGYL LDQFLQDSAN RRTDAYGGSI ENRARLLLEV VDAAIDVWSA ARVGVHLAPR GDAHTMGDSD PAATFGHVAR ELGRRRIAFL FARESFGGDA IGQQLKAAFG GPFIVNENFT LDSAQAALDA GQADAVAWGK LFIANPDLPR RFKLNAPLNE PNAATFYAQG EVGYTDYPAL ESAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctt ctctgttcga tccgctgacc atcggcgacc tcacactggc gaaccgcatc atcatggcgc cgctcacgcg cgcccgcgcc ggcgacacgc gcacgcccaa cgcgctgatg gcgcgctact acgcggagcg cgcatccgcg gggctcatca tcagcgaggc gacatcggtc acgccgcaag gcgtcggtta cgcaagcacg ccgggcatct ggtcgcccga acaggtcgac gggtggcgac tcgtgacgga cgcggtccac gcggccggcg gccgcatctt cctgcagctc tggcacgtcg gccgcgtgtc ggatccggtg ttcctcgacg gcgcgctgcc cgtcgcgccg agcgcgatcg cccccggcgg ccacgtgagc ctcgtgcgcc cgcagcggcc gtacgtgacg ccgcgcgcgc tcgagctcga cgagattccg ggcgtcgtcg ccgcgttccg ccgcggcgcg gaaaacgcgc gcgcggccgg cttcgacggc gtcgaggtac acggcgcgaa cggctatctg ctcgatcaat tcctgcagga cagcgcgaac cgccgcaccg acgcgtacgg cggctcgatc gagaaccgcg cgcgcctgtt gctcgaggtg gtcgacgcgg cgatcgacgt ctggagcgcg gcgcgcgtcg gcgtgcatct cgcgccgcgc ggcgatgcgc acacgatggg cgattcggac cccgcggcta cgttcggcca cgtcgcgcgc gaactcggcc gccgccgcat cgcgttcctg ttcgcacgcg aatcgttcgg cggcgacgcc atcggccagc aattgaaggc ggcgttcggc gggccattca tcgtgaacga gaatttcacg ctcgacagcg cgcaggcggc gctcgacgcg ggacaggcgg acgcggtcgc gtggggcaag ctttttatcg cgaatccgga cctgccgcgc cgcttcaagc tgaatgcgcc gctcaacgag ccgaatgccg ctacgttcta tgcgcaaggc gaagtaggct atacggacta tccggcgctg gaaagcgcgg cgtaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSLFDPLT IGDLTLANRI IMAPLTRARA GDTRTPNALM ARYYAERASA GLIISEATSV TPQGVGYAST PGIWSPEQVD GWRLVTDAVH AAGGRIFLQL WHVGRVSDPV FLDGALPVAP SAIAPGGHVS LVRPQRPYVT PRALELDEIP GVVAAFRRGA ENARAAGFDG VEVHGANGYL LDQFLQDSAN RRTDAYGGSI ENRARLLLEV VDAAIDVWSA ARVGVHLAPR GDAHTMGDSD PAATFGHVAR ELGRRRIAFL FARESFGGDA IGQQLKAAFG GPFIVNENFT LDSAQAALDA GQADAVAWGK LFIANPDLPR RFKLNAPLNE PNAATFYAQG EVGYTDYPAL ESAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccttctctg ttcgatccgc tgaccatcgg cgacctcaca ctggcgaacc gcatcatcat ggcgccgctc acgcgcgccc gcgccggcga cacgcgcacg cccaacgcgc tgatggcgcg ctactacgcg gagcgcgcat ccgcggggct catcatcagc gaggcgacat cggtcacgcc gcaaggcgtc ggttacgcaa gcacgccggg catctggtcg cccgaacagg tcgacgggtg gcgactcgtg acggacgcgg tccacgcggc cggcggccgc atcttcctgc agctctggca cgtcggccgc gtgtcggatc cggtgttcct cgacggcgcg ctgcccgtcg cgccgagcgc gatcgccccc ggcggccacg tgagcctcgt gcgcccgcag cggccgtacg tgacgccgcg cgcgctcgag ctcgacgaga ttccgggcgt cgtcgccgcg ttccgccgcg gcgcggaaaa cgcgcgcgcg gccggcttcg acggcgtcga ggtacacggc gcgaacggct atctgctcga tcaattcctg caggacagcg cgaaccgccg caccgacgcg tacggcggct cgatcgagaa ccgcgcgcgc ctgttgctcg aggtggtcga cgcggcgatc gacgtctgga gcgcggcgcg cgtcggcgtg catctcgcgc cgcgcggcga tgcgcacacg atgggcgatt cggaccccgc ggctacgttc ggccacgtcg cgcgcgaact cggccgccgc cgcatcgcgt tcctgttcgc acgcgaatcg ttcggcggcg acgccatcgg ccagcaattg aaggcggcgt tcggcgggcc attcatcgtg aacgagaatt tcacgctcga cagcgcgcag gcggcgctcg acgcgggaca ggcggacgcg gtcgcgtggg gcaagctttt tatcgcgaat ccggacctgc cgcgccgctt caagctgaat gcgccgctca acgagccgaa tgccgctacg ttctatgcgc aaggcgaagt aggctatacg gactatccgg cgctggaaag cgcggcgtga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.00093.a.A1.PS00075
| PURIFICATION DATe: | 8/23/2008 |
| CONCENTRATION: | 12.17mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSLFDPLT IGDLTLANRI IMAPLTRARA GDTRTPNALM ARYYAERASA GLIISEATSV TPQGVGYAST PGIWSPEQVD GWRLVTDAVH AAGGRIFLQL WHVGRVSDPV FLDGALPVAP SAIAPGGHVS LVRPQRPYVT PRALELDEIP GVVAAFRRGA ENARAAGFDG VEVHGANGYL LDQFLQDSAN RRTDAYGGSI ENRARLLLEV VDAAIDVWSA ARVGVHLAPR GDAHTMGDSD PAATFGHVAR ELGRRRIAFL FARESFGGDA IGQQLKAAFG GPFIVNENFT LDSAQAALDA GQADAVAWGK LFIANPDLPR RFKLNAPLNE PNAATFYAQG EVGYTDYPAL ESAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctt ctctgttcga tccgctgacc atcggcgacc tcacactggc gaaccgcatc atcatggcgc cgctcacgcg cgcccgcgcc ggcgacacgc gcacgcccaa cgcgctgatg gcgcgctact acgcggagcg cgcatccgcg gggctcatca tcagcgaggc gacatcggtc acgccgcaag gcgtcggtta cgcaagcacg ccgggcatct ggtcgcccga acaggtcgac gggtggcgac tcgtgacgga cgcggtccac gcggccggcg gccgcatctt cctgcagctc tggcacgtcg gccgcgtgtc ggatccggtg ttcctcgacg gcgcgctgcc cgtcgcgccg agcgcgatcg cccccggcgg ccacgtgagc ctcgtgcgcc cgcagcggcc gtacgtgacg ccgcgcgcgc tcgagctcga cgagattccg ggcgtcgtcg ccgcgttccg ccgcggcgcg gaaaacgcgc gcgcggccgg cttcgacggc gtcgaggtac acggcgcgaa cggctatctg ctcgatcaat tcctgcagga cagcgcgaac cgccgcaccg acgcgtacgg cggctcgatc gagaaccgcg cgcgcctgtt gctcgaggtg gtcgacgcgg cgatcgacgt ctggagcgcg gcgcgcgtcg gcgtgcatct cgcgccgcgc ggcgatgcgc acacgatggg cgattcggac cccgcggcta cgttcggcca cgtcgcgcgc gaactcggcc gccgccgcat cgcgttcctg ttcgcacgcg aatcgttcgg cggcgacgcc atcggccagc aattgaaggc ggcgttcggc gggccattca tcgtgaacga gaatttcacg ctcgacagcg cgcaggcggc gctcgacgcg ggacaggcgg acgcggtcgc gtggggcaag ctttttatcg cgaatccgga cctgccgcgc cgcttcaagc tgaatgcgcc gctcaacgag ccgaatgccg ctacgttcta tgcgcaaggc gaagtaggct atacggacta tccggcgctg gaaagcgcgg cgtaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSLFDPLT IGDLTLANRI IMAPLTRARA GDTRTPNALM ARYYAERASA GLIISEATSV TPQGVGYAST PGIWSPEQVD GWRLVTDAVH AAGGRIFLQL WHVGRVSDPV FLDGALPVAP SAIAPGGHVS LVRPQRPYVT PRALELDEIP GVVAAFRRGA ENARAAGFDG VEVHGANGYL LDQFLQDSAN RRTDAYGGSI ENRARLLLEV VDAAIDVWSA ARVGVHLAPR GDAHTMGDSD PAATFGHVAR ELGRRRIAFL FARESFGGDA IGQQLKAAFG GPFIVNENFT LDSAQAALDA GQADAVAWGK LFIANPDLPR RFKLNAPLNE PNAATFYAQG EVGYTDYPAL ESAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTTCTCTG TTCGATCCGC TGACCATCGG CGACCTCACA CTGGCGAACC GCATCATCAT GGCGCCGCTC ACGCGCGCCC GCGCCGGCGA CACGCGCACG CCCAACGCGC TGATGGCGCG CTACTACGCG GAGCGCGCAT CCGCGGGGCT CATCATCAGC GAGGCGACAT CGGTCACGCC GCAAGGCGTC GGTTACGCAA GCACGCCGGG CATCTGGTCG CCCGAACAGG TCGACGGGTG GCGACTCGTG ACGGACGCGG TCCACGCGGC CGGCGGCCGC ATCTTCCTGC AGCTCTGGCA CGTCGGCCGC GTGTCGGATC CGGTGTTCCT CGACGGCGCG CTGCCCGTCG CGCCGAGCGC GATCGCCCCC GGCGGCCACG TGAGCCTCGT GCGCCCGCAG CGGCCGTACG TGACGCCGCG CGCGCTCGAG CTCGACGAGA TTCCGGGCGT CGTCGCCGCG TTCCGCCGCG GCGCGGAAAA CGCGCGCGCG GCCGGCTTCG ACGGCGTCGA GGTACACGGC GCGAACGGCT ATCTGCTCGA TCAATTCCTG CAGGACAGCG CGAACCGCCG CACCGACGCG TACGGCGGCT CGATCGAGAA CCGCGCGCGC CTGTTGCTCG AGGTGGTCGA CGCGGCGATC GACGTCTGGA GCGCGGCGCG CGTCGGCGTG CATCTCGCGC CGCGCGGCGA TGCGCACACG ATGGGCGATT CGGACCCCGC GGCTACGTTC GGCCACGTCG CGCGCGAACT CGGCCGCCGC CGCATCGCGT TCCTGTTCGC ACGCGAATCG TTCGGCGGCG ACGCCATCGG CCAGCAATTG AAGGCGGCGT TCGGCGGGCC ATTCATCGTG AACGAGAATT TCACGCTCGA CAGCGCGCAG GCGGCGCTCG ACGCGGGACA GGCGGACGCG GTCGCGTGGG GCAAGCTTTT TATCGCGAAT CCGGACCTGC CGCGCCGCTT CAAGCTGAAT GCGCCGCTCA ACGAGCCGAA TGCCGCTACG TTCTATGCGC AAGGCGAAGT AGGCTATACG GACTATCCGG CGCTGGAAAG CGCGGCGTGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BupsA.00093.a.B1.PS00139
| PURIFICATION DATe: | 10/30/2008 |
| CONCENTRATION: | 47.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 150µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 101 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP SLFDPLTIGD LTLANRIIMA PLTRARAGDT RTPNALMARY YAERASAGLI ISEATSVTPQ GVGYASTPGI WSPEQVDGWR LVTDAVHAAG GRIFLQLWHV GRVSDPVFLD GALPVAPSAI APGGHVSLVR PQRPYVTPRA LELDEIPGVV AAFRRGAENA RAAGFDGVEV HGANGYLLDQ FLQDSANRRT DAYGGSIENR ARLLLEVVDA AIDVWSAARV GVHLAPRGDA HTMGDSDPAX TFGHVARELG RRRIAFLFAR ESFGGDAXGQ QLKXAFGGPF IVNENFTLDS AXAALDAGQA DAVAWGKLFI XNPDLPRRXK LNAXLNEPNX XTFYAQGEVG YTDXXALESA AXXSK
Validated NT Sequence
atggctcacc accaccacca ccatatgcct tctctgttcg atccgctgac catcggcgac ctcacactgg cgaaccgcat catcatggcg ccgctcacgc gcgcccgcgc cggcgacacg cgcacgccca acgcgctgat ggcgcgctac tacgcggagc gcgcatccgc ggggctcatc atcagcgagg cgacatcggt cacgccgcaa ggcgtcggtt acgcaagcac gccgggcatc tggtcgcccg aacaggtcga cgggtggcga ctcgtgacgg acgcggtcca cgcggccggc ggccgcatct tcctgcagct ctggcacgtc ggccgcgtgt cggatccggt gttcctcgac ggcgcgctgc ccgtcgcgcc gagcgcgatc gcccccggcg gccacgtgag cctcgtgcgc ccgcagcggc cgtacgtgac gccgcgcgcg ctcgagctcg acgagattcc gggcgtcgtc gccgcgttcc gccgcggcgc ggaaaacgcg cgcgcggccg gcttcgacgg cgtcgaggta cacggcgcga acggctatct gctcgatcaa ttcctgcagg acagcgcgaa ccgccgcacc gacgcgtacg gcggctcgat cgagaaccgc gcgcgcctgt tgctcgaggt ggtcgacgcg gcgatcgacg tctggagcgc ggcgcgcgtc ggcgtgcatc tcgcgccgcg cggcgatgcg cacacgatgg gcgattcgga ccccgcggnt acgttcggcc acgtcgcgcg cgaactcggc cgccgccgca tcgcgttcct gttcgcacgc gaatcgttcg gcggcgacgc cntcggccag caattgaagg nggcgttcgg cgggccattc atcgtgaacg agaatttcac gctcgacagc gcgcnggcgg cgctcgacgc gggacaggcg gacgcggtcg cgtggggcaa gctttttatc nngaatccgg acctgccgcg ccgctncaag ctgaatgcnn cgctcaacga gccgaatgnn nntacgttct atgcgcaagg cgaagtaggc tatacggact ancnggcgct ggaaagcgcg gcntnngnna gtaagata
Expressed Protein Sequence
MAHHHHHHMP SLFDPLTIGD LTLANRIIMA PLTRARAGDT RTPNALMARY YAERASAGLI ISEATSVTPQ GVGYASTPGI WSPEQVDGWR LVTDAVHAAG GRIFLQLWHV GRVSDPVFLD GALPVAPSAI APGGHVSLVR PQRPYVTPRA LELDEIPGVV AAFRRGAENA RAAGFDGVEV HGANGYLLDQ FLQDSANRRT DAYGGSIENR ARLLLEVVDA AIDVWSAARV GVHLAPRGDA HTMGDSDPAA TFGHVARELG RRRIAFLFAR ESFGGDAIGQ QLKAAFGGPF IVNENFTLDS AQAALDAGQA DAVAWGKLFI ANPDLPRRFK LNAPLNEPNA ATFYAQGEVG YTDYPALESA A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTT CTCTGTTCGA TCCGCTGACC ATCGGCGACC TCACACTGGC GAACCGCATC ATCATGGCGC CGCTCACGCG CGCCCGCGCC GGCGACACGC GCACGCCCAA CGCGCTGATG GCGCGCTACT ACGCGGAGCG CGCATCCGCG GGGCTCATCA TCAGCGAGGC GACATCGGTC ACGCCGCAAG GCGTCGGTTA CGCAAGCACG CCGGGCATCT GGTCGCCCGA ACAGGTCGAC GGGTGGCGAC TCGTGACGGA CGCGGTCCAC GCGGCCGGCG GCCGCATCTT CCTGCAGCTC TGGCACGTCG GCCGCGTGTC GGATCCGGTG TTCCTCGACG GCGCGCTGCC CGTCGCGCCG AGCGCGATCG CCCCCGGCGG CCACGTGAGC CTCGTGCGCC CGCAGCGGCC GTACGTGACG CCGCGCGCGC TCGAGCTCGA CGAGATTCCG GGCGTCGTCG CCGCGTTCCG CCGCGGCGCG GAAAACGCGC GCGCGGCCGG CTTCGACGGC GTCGAGGTAC ACGGCGCGAA CGGCTATCTG CTCGATCAAT TCCTGCAGGA CAGCGCGAAC CGCCGCACCG ACGCGTACGG CGGCTCGATC GAGAACCGCG CGCGCCTGTT GCTCGAGGTG GTCGACGCGG CGATCGACGT CTGGAGCGCG GCGCGCGTCG GCGTGCATCT CGCGCCGCGC GGCGATGCGC ACACGATGGG CGATTCGGAC CCCGCGGCTA CGTTCGGCCA CGTCGCGCGC GAACTCGGCC GCCGCCGCAT CGCGTTCCTG TTCGCACGCG AATCGTTCGG CGGCGACGCC ATCGGCCAGC AATTGAAGGC GGCGTTCGGC GGGCCATTCA TCGTGAACGA GAATTTCACG CTCGACAGCG CGCAGGCGGC GCTCGACGCG GGACAGGCGG ACGCGGTCGC GTGGGGCAAG CTTTTTATCG CGAATCCGGA CCTGCCGCGC CGCTTCAAGC TGAATGCGCC GCTCAACGAG CCGAATGCCG CTACGTTCTA TGCGCAAGGC GAAGTAGGCT ATACGGACTA TCCGGCGCTG GAAAGCGCGG CGTGATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for BupsA.00093.a.A1.PW25093
| PURIFICATION DATe: | 5/1/2008 |
| CONCENTRATION: | 30.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 28 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSLFDPLT IGDLTLANRI IMAPLTRARA GDTRTPNALM ARYYAERASA GLIISEATSV TPQGVGYAST PGIWSPEQVD GWRLVTDAVH AAGGRIFLQL WHVGRVSDPV FLDGALPVAP SAIAPGGHVS LVRPQRPYVT PRALELDEIP GVVAAFRRGA ENARAAGFDG VEVHGANGYL LDQFLQDSAN RRTDAYGGSI ENRARLLLEV VDAAIDVWSA ARVGVHLAPR GDAHTMGDSD PAATFGHVAR ELGRRRIAFL FARESFGGDA IGQQLKAAFG GPFIVNENFT LDSAQAALDA GQADAVAWGK LFIANPDLPR RFKLNAPLNE PNAATFYAQG EVGYTDYPAL ESAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctt ctctgttcga tccgctgacc atcggcgacc tcacactggc gaaccgcatc atcatggcgc cgctcacgcg cgcccgcgcc ggcgacacgc gcacgcccaa cgcgctgatg gcgcgctact acgcggagcg cgcatccgcg gggctcatca tcagcgaggc gacatcggtc acgccgcaag gcgtcggtta cgcaagcacg ccgggcatct ggtcgcccga acaggtcgac gggtggcgac tcgtgacgga cgcggtccac gcggccggcg gccgcatctt cctgcagctc tggcacgtcg gccgcgtgtc ggatccggtg ttcctcgacg gcgcgctgcc cgtcgcgccg agcgcgatcg cccccggcgg ccacgtgagc ctcgtgcgcc cgcagcggcc gtacgtgacg ccgcgcgcgc tcgagctcga cgagattccg ggcgtcgtcg ccgcgttccg ccgcggcgcg gaaaacgcgc gcgcggccgg cttcgacggc gtcgaggtac acggcgcgaa cggctatctg ctcgatcaat tcctgcagga cagcgcgaac cgccgcaccg acgcgtacgg cggctcgatc gagaaccgcg cgcgcctgtt gctcgaggtg gtcgacgcgg cgatcgacgt ctggagcgcg gcgcgcgtcg gcgtgcatct cgcgccgcgc ggcgatgcgc acacgatggg cgattcggac cccgcggcta cgttcggcca cgtcgcgcgc gaactcggcc gccgccgcat cgcgttcctg ttcgcacgcg aatcgttcgg cggcgacgcc atcggccagc aattgaaggc ggcgttcggc gggccattca tcgtgaacga gaatttcacg ctcgacagcg cgcaggcggc gctcgacgcg ggacaggcgg acgcggtcgc gtggggcaag ctttttatcg cgaatccgga cctgccgcgc cgcttcaagc tgaatgcgcc gctcaacgag ccgaatgccg ctacgttcta tgcgcaaggc gaagtaggct atacggacta tccggcgctg gaaagcgcgg cgtaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSLFDPLT IGDLTLANRI IMAPLTRARA GDTRTPNALM ARYYAERASA GLIISEATSV TPQGVGYAST PGIWSPEQVD GWRLVTDAVH AAGGRIFLQL WHVGRVSDPV FLDGALPVAP SAIAPGGHVS LVRPQRPYVT PRALELDEIP GVVAAFRRGA ENARAAGFDG VEVHGANGYL LDQFLQDSAN RRTDAYGGSI ENRARLLLEV VDAAIDVWSA ARVGVHLAPR GDAHTMGDSD PAATFGHVAR ELGRRRIAFL FARESFGGDA IGQQLKAAFG GPFIVNENFT LDSAQAALDA GQADAVAWGK LFIANPDLPR RFKLNAPLNE PNAATFYAQG EVGYTDYPAL ESAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTTCTCTG TTCGATCCGC TGACCATCGG CGACCTCACA CTGGCGAACC GCATCATCAT GGCGCCGCTC ACGCGCGCCC GCGCCGGCGA CACGCGCACG CCCAACGCGC TGATGGCGCG CTACTACGCG GAGCGCGCAT CCGCGGGGCT CATCATCAGC GAGGCGACAT CGGTCACGCC GCAAGGCGTC GGTTACGCAA GCACGCCGGG CATCTGGTCG CCCGAACAGG TCGACGGGTG GCGACTCGTG ACGGACGCGG TCCACGCGGC CGGCGGCCGC ATCTTCCTGC AGCTCTGGCA CGTCGGCCGC GTGTCGGATC CGGTGTTCCT CGACGGCGCG CTGCCCGTCG CGCCGAGCGC GATCGCCCCC GGCGGCCACG TGAGCCTCGT GCGCCCGCAG CGGCCGTACG TGACGCCGCG CGCGCTCGAG CTCGACGAGA TTCCGGGCGT CGTCGCCGCG TTCCGCCGCG GCGCGGAAAA CGCGCGCGCG GCCGGCTTCG ACGGCGTCGA GGTACACGGC GCGAACGGCT ATCTGCTCGA TCAATTCCTG CAGGACAGCG CGAACCGCCG CACCGACGCG TACGGCGGCT CGATCGAGAA CCGCGCGCGC CTGTTGCTCG AGGTGGTCGA CGCGGCGATC GACGTCTGGA GCGCGGCGCG CGTCGGCGTG CATCTCGCGC CGCGCGGCGA TGCGCACACG ATGGGCGATT CGGACCCCGC GGCTACGTTC GGCCACGTCG CGCGCGAACT CGGCCGCCGC CGCATCGCGT TCCTGTTCGC ACGCGAATCG TTCGGCGGCG ACGCCATCGG CCAGCAATTG AAGGCGGCGT TCGGCGGGCC ATTCATCGTG AACGAGAATT TCACGCTCGA CAGCGCGCAG GCGGCGCTCG ACGCGGGACA GGCGGACGCG GTCGCGTGGG GCAAGCTTTT TATCGCGAAT CCGGACCTGC CGCGCCGCTT CAAGCTGAAT GCGCCGCTCA ACGAGCCGAA TGCCGCTACG TTCTATGCGC AAGGCGAAGT AGGCTATACG GACTATCCGG CGCTGGAAAG CGCGGCGTGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA