BupsA.00092.a
isocitrate dehydrogenase
| CENTER ID: | BupsA.00092.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00092.a.A1.PW25020 | Full length( BupsA.00092.a ) | 1 | 419 | ||
| BupsA.00092.a.A1.PW25104 | Full length( BupsA.00092.a ) | 1 | 419 | ||
| BupsA.00092.a.B1.PW25188 | Full length( BupsA.00092.a ) | 1 | 419 |
Structures
3DMS
DEPOSITED: 7/1/2008
DETERMINATION: XRay
CLONE: BupsA.00092.a.B1.GE30977
PROTEIN: BupsA.00092.a.B1.PS00006
Publications by SSGCID
Structural basis of the substrate specificity of bifunctional isocitrate dehydrogenase kinase/phosphatase.
Bryan CM, Edwards TE, Jia Z, Myler PJ, Stein AJ, Stewart LJ, Van Voorhis WC, Yates SP, Zheng J
Biochemistry - 2011
volume 50, issue 38, pages 8103-6
PMID: 21870819; PMCID: PMC3354702
PDB:
3DMS
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126870 |
| PATRIC ID: | fig|320372.6.peg.1126 |
| RefSeq: | YP_332521 |
| UniProt: | Q3JV82 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPYQHIKVPE GGDKITVNKD FSLNVSDQPI IPYIEGDGTG FDITPVMIKV VDAAVEKAYG GKKKIHWMEI YAGEKATKVY GPDVWLPEET LQVLKEYVVS IKGPLTTPVG GGIRSLNVAL RQELDLYVCL RPIQYFKGVP SPVREPEKTN MVIFRENSED IYAGIEWAAE SEQAKKVIKF LQEEMGVKKI RFPQTSGIGI KPVSKEGTER LVRKAIQYAI DNDRKSVTLV HKGNIMKFTE GAFRDAGYAL AQKEFGAELI DGGPWMKFKN PKTGNEIVVK DSIADAFLQQ ILLRPAEYDV IATLNLNGDY ISDALAAQVG GIGIAPGANL SDSVAMFEAT HGTAPKYAGK DYVNPGSEIL SAEMMLRHLG WTEAADVIIS AMEKSIKQKR VTYDFARLME GATQVSCSGF GQVLIENME
NT Sequence
ATGCCGTATC AGCACATCAA GGTTCCGGAA GGCGGTGACA AGATCACCGT CAACAAGGAT TTCTCGCTCA ACGTTTCCGA TCAGCCGATC ATCCCTTACA TCGAGGGCGA CGGCACGGGC TTCGACATCA CGCCGGTGAT GATCAAGGTC GTCGACGCGG CGGTCGAGAA GGCGTACGGC GGCAAGAAGA AGATTCACTG GATGGAGATC TACGCCGGCG AGAAGGCGAC GAAGGTCTAC GGCCCGGACG TGTGGCTGCC GGAAGAGACG CTGCAGGTGC TGAAGGAATA CGTCGTGTCG ATCAAGGGGC CGCTCACGAC CCCGGTCGGC GGCGGCATCC GCTCGCTGAA CGTCGCGCTG CGTCAGGAGC TCGACCTGTA CGTCTGCCTG CGCCCGATCC AGTACTTCAA GGGCGTGCCT TCGCCCGTGC GCGAGCCGGA GAAGACCAAC ATGGTCATCT TCCGCGAGAA CTCGGAAGAC ATCTACGCCG GCATCGAATG GGCGGCCGAA TCCGAGCAGG CGAAGAAGGT CATCAAGTTC CTGCAGGAAG AGATGGGCGT GAAGAAGATC CGCTTCCCGC AGACCTCGGG CATCGGCATC AAGCCCGTGT CGAAGGAAGG CACCGAGCGC CTCGTGCGCA AGGCGATCCA GTACGCGATC GACAACGACC GCAAGTCGGT CACGCTCGTC CACAAGGGCA ACATCATGAA GTTCACGGAA GGTGCGTTCC GTGACGCCGG CTATGCGCTC GCGCAGAAGG AATTCGGCGC CGAGCTGATC GACGGCGGCC CGTGGATGAA GTTCAAGAAC CCGAAGACGG GCAACGAGAT CGTGGTCAAG GATTCGATCG CCGACGCGTT CCTGCAGCAG ATCCTGCTGC GCCCGGCCGA ATACGACGTG ATCGCGACGC TGAACCTGAA CGGCGACTAC ATCTCGGACG CGCTCGCCGC GCAAGTCGGC GGCATCGGCA TCGCGCCGGG CGCGAACCTG TCGGATTCGG TCGCGATGTT CGAAGCGACG CACGGCACGG CGCCGAAGTA CGCGGGCAAG GATTACGTGA ACCCGGGTTC GGAAATCCTG TCGGCCGAAA TGATGCTGCG CCACCTCGGC TGGACCGAAG CGGCCGACGT GATCATTTCC GCGATGGAGA AGTCGATCAA GCAGAAGCGC GTCACGTACG ACTTCGCGCG CCTGATGGAA GGCGCGACGC AAGTGTCGTG CTCGGGCTTC GGTCAGGTGC TGATCGAAAA CATGGAG
Details for BupsA.00092.a.A1.PW25020
| PURIFICATION DATe: | 4/7/2008 |
| CONCENTRATION: | 29mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 20 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPYQHIKVP EGGDKITVNK DFSLNVSDQP IIPYIEGDGT GFDITPVMIK VVDAAVEKAY GGKKKIHWME IYAGEKATKV YGPDVWLPEE TLQVLKEYVV SIKGPLTTPV GGGIRSLNVA LRQELDLYVC LRPIQYFKGV PSPVREPEKT NMVIFRENSE DIYAGIEWAA ESEQAKKVIK FLQEEMGVKK IRFPQTSGIG IKPVSKEGTE RLVRKAIQYA IDNDRKSVTL VHKGNIMKFT EGAFRDAGYA LAQKEFGAEL IDGGPWMKFK NPKTGNEIVV KDSIADAFLQ QILLRPAEYD VIATLNLNGD YISDALAAQV GGIGIAPGAN LSDSVAMFEA THGTAPKYAG KDYVNPGSEI LSAEMMLRHL GWTEAADVII SAMEKSIKQK RVTYDFARLM EGATQVSCSG FGQVLIENME
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGTATCAG CACATCAAGG TTCCGGAAGG CGGTGACAAG ATCACCGTCA ACAAGGATTT CTCGCTCAAC GTTTCCGATC AGCCGATCAT CCCTTACATC GAGGGCGACG GCACGGGCTT CGACATCACG CCGGTGATGA TCAAGGTCGT CGACGCGGCG GTCGAGAAGG CGTACGGCGG CAAGAAGAAG ATTCACTGGA TGGAGATCTA CGCCGGCGAG AAGGCGACGA AGGTCTACGG CCCGGACGTG TGGCTGCCGG AAGAGACGCT GCAGGTGCTG AAGGAATACG TCGTGTCGAT CAAGGGGCCG CTCACGACCC CGGTCGGCGG CGGCATCCGC TCGCTGAACG TCGCGCTGCG TCAGGAGCTC GACCTGTACG TCTGCCTGCG CCCGATCCAG TACTTCAAGG GCGTGCCTTC GCCCGTGCGC GAGCCGGAGA AGACCAACAT GGTCATCTTC CGCGAGAACT CGGAAGACAT CTACGCCGGC ATCGAATGGG CGGCCGAATC CGAGCAGGCG AAGAAGGTCA TCAAGTTCCT GCAGGAAGAG ATGGGCGTGA AGAAGATCCG CTTCCCGCAG ACCTCGGGCA TCGGCATCAA GCCCGTGTCG AAGGAAGGCA CCGAGCGCCT CGTGCGCAAG GCGATCCAGT ACGCGATCGA CAACGACCGC AAGTCGGTCA CGCTCGTCCA CAAGGGCAAC ATCATGAAGT TCACGGAAGG TGCGTTCCGT GACGCCGGCT ATGCGCTCGC GCAGAAGGAA TTCGGCGCCG AGCTGATCGA CGGCGGCCCG TGGATGAAGT TCAAGAACCC GAAGACGGGC AACGAGATCG TGGTCAAGGA TTCGATCGCC GACGCGTTCC TGCAGCAGAT CCTGCTGCGC CCGGCCGAAT ACGACGTGAT CGCGACGCTG AACCTGAACG GCGACTACAT CTCGGACGCG CTCGCCGCGC AAGTCGGCGG CATCGGCATC GCGCCGGGCG CGAACCTGTC GGATTCGGTC GCGATGTTCG AAGCGACGCA CGGCACGGCG CCGAAGTACG CGGGCAAGGA TTACGTGAAC CCGGGTTCGG AAATCCTGTC GGCCGAAATG ATGCTGCGCC ACCTCGGCTG GACCGAAGCG GCCGACGTGA TCATTTCCGC GATGGAGAAG TCGATCAAGC AGAAGCGCGT CACGTACGAC TTCGCGCGCC TGATGGAAGG CGCGACGCAA GTGTCGTGCT CGGGCTTCGG TCAGGTGCTG ATCGAAAACA TGGAGTAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BupsA.00092.a.A1.PW25104
| PURIFICATION DATe: | 3/21/2008 |
| CONCENTRATION: | data unavailable |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 58 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPYQHIKVP EGGDKITVNK DFSLNVSDQP IIPYIEGDGT GFDITPVMIK VVDAAVEKAY GGKKKIHWME IYAGEKATKV YGPDVWLPEE TLQVLKEYVV SIKGPLTTPV GGGIRSLNVA LRQELDLYVC LRPIQYFKGV PSPVREPEKT NMVIFRENSE DIYAGIEWAA ESEQAKKVIK FLQEEMGVKK IRFPQTSGIG IKPVSKEGTE RLVRKAIQYA IDNDRKSVTL VHKGNIMKFT EGAFRDAGYA LAQKEFGAEL IDGGPWMKFK NPKTGNEIVV KDSIADAFLQ QILLRPAEYD VIATLNLNGD YISDALAAQV GGIGIAPGAN LSDSVAMFEA THGTAPKYAG KDYVNPGSEI LSAEMMLRHL GWTEAADVII SAMEKSIKQK RVTYDFARLM EGATQVSCSG FGQVLIENME
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGTATCAG CACATCAAGG TTCCGGAAGG CGGTGACAAG ATCACCGTCA ACAAGGATTT CTCGCTCAAC GTTTCCGATC AGCCGATCAT CCCTTACATC GAGGGCGACG GCACGGGCTT CGACATCACG CCGGTGATGA TCAAGGTCGT CGACGCGGCG GTCGAGAAGG CGTACGGCGG CAAGAAGAAG ATTCACTGGA TGGAGATCTA CGCCGGCGAG AAGGCGACGA AGGTCTACGG CCCGGACGTG TGGCTGCCGG AAGAGACGCT GCAGGTGCTG AAGGAATACG TCGTGTCGAT CAAGGGGCCG CTCACGACCC CGGTCGGCGG CGGCATCCGC TCGCTGAACG TCGCGCTGCG TCAGGAGCTC GACCTGTACG TCTGCCTGCG CCCGATCCAG TACTTCAAGG GCGTGCCTTC GCCCGTGCGC GAGCCGGAGA AGACCAACAT GGTCATCTTC CGCGAGAACT CGGAAGACAT CTACGCCGGC ATCGAATGGG CGGCCGAATC CGAGCAGGCG AAGAAGGTCA TCAAGTTCCT GCAGGAAGAG ATGGGCGTGA AGAAGATCCG CTTCCCGCAG ACCTCGGGCA TCGGCATCAA GCCCGTGTCG AAGGAAGGCA CCGAGCGCCT CGTGCGCAAG GCGATCCAGT ACGCGATCGA CAACGACCGC AAGTCGGTCA CGCTCGTCCA CAAGGGCAAC ATCATGAAGT TCACGGAAGG TGCGTTCCGT GACGCCGGCT ATGCGCTCGC GCAGAAGGAA TTCGGCGCCG AGCTGATCGA CGGCGGCCCG TGGATGAAGT TCAAGAACCC GAAGACGGGC AACGAGATCG TGGTCAAGGA TTCGATCGCC GACGCGTTCC TGCAGCAGAT CCTGCTGCGC CCGGCCGAAT ACGACGTGAT CGCGACGCTG AACCTGAACG GCGACTACAT CTCGGACGCG CTCGCCGCGC AAGTCGGCGG CATCGGCATC GCGCCGGGCG CGAACCTGTC GGATTCGGTC GCGATGTTCG AAGCGACGCA CGGCACGGCG CCGAAGTACG CGGGCAAGGA TTACGTGAAC CCGGGTTCGG AAATCCTGTC GGCCGAAATG ATGCTGCGCC ACCTCGGCTG GACCGAAGCG GCCGACGTGA TCATTTCCGC GATGGAGAAG TCGATCAAGC AGAAGCGCGT CACGTACGAC TTCGCGCGCC TGATGGAAGG CGCGACGCAA GTGTCGTGCT CGGGCTTCGG TCAGGTGCTG ATCGAAAACA TGGAGTAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BupsA.00092.a.B1.PW25188
| PURIFICATION DATe: | 10/18/2010 |
| CONCENTRATION: | 20.91mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP YQHIKVPEGG DKITVNKDFS LNVSDQPIIP YIEGDGTGFD ITPVMIKVVD AAVEKAYGGK KKIHWMEIYA GEKATKVYGP DVWLPEETLQ VLKEYVVSIK GPLTTPVGGG IRSLNVALRQ ELDLYVCLRP IQYFKGVPSP VREPEKTNMV IFRENSEDIY AGIEWAAESE QAKKVIKFLQ EEMGVKKIRF PQTSGIGIKP VSKEGTERLV RKAIQYAIDN DRKSVTLVHK GNIMKFTEGA FRDAGYALAQ KEFGAELIDG GPWMKFKNPK TGNEIVVKDS IADAFLQQIL LRPAEYDVIA TLNLNGDYIS DALAAQVGGI GIAPGANLSD SVAMFEATHG TAPKYAGKDY VNPGSEILSA EMMLRHLGWT EAADVIISAM EKSIKQKRVT YDFARLMEGA TQVSCSGFGQ VLIENME
Validated NT Sequence
atcttactca cttactccat gttttcgatc agcacctgac cgaagcccga gcacgacact tgcgtcgcgc cttccatcag gcgcgcgaag tcgtacgtga cgcgcttctg cttgatcgac ttctccatcg cggaaatgat cacgtcggcc gcttcggtcc agccgaggtg gcgcagcatc atttcggccg acaggatttc cgaacccggg ttcacgtaat ccttgcccgc gtacttcggc gccgtgccgt gcgtcgcttc gaacatcgcg accgaatccg acaggttcgc gcccggcgcg atgccgatgc cgccgacttg cgcggcgagc gcgtccgaga tgtagtcgcc gttcaggttc agcgtcgcga tcacgtcgta ttcggccggg cgcagcagga tctgctgcag gaacgcgtcg gcgatcgaat ccttgaccac gatctcgttg cccgtcttcg ggttcttgaa cttcatccac gggccgccgt cgatcagctc ggcgccgaat tccttctgcg cgagcgcata gccggcgtca cggaacgcac cttccgtgaa cttcatgatg ttgcccttgt ggacgagcgt gaccgacttg cggtcgttgt cgatcgcgta ctggatcgcc ttgcgcacga ggcgctcggt gccttccttc gacacgggct tgatgccgat gcccgaggtc tgcgggaagc ggatcttctt cacgcccatc tcttcctgca ggaacttgat gaccttcttc gcctgctcgg attcggccgc ccattcgatg ccggcgtaga tgtcttccga gttctcgcgg aagatgacca tgttggtctt ctccggctcg cgcacgggcg aaggcacgcc cttgaagtac tggatcgggc gcaggcagac gtacaggtcg agctcctgac gcagcgcgac gttcagcgag cggatgccgc cgccgaccgg ggtcgtgagc ggccccttga tcgacacgac gtattccttc agcacctgca gcgtctcttc cggcagccac acgtccgggc cgtagacctt cgtcgccttc tcgccggcgt agatctccat ccagtgaatc ttcttcttgc cgccgtacgc cttctcgacc gccgcgtcga cgaccttgat catcaccggc gtgatgtcga agcccgtgcc gtcgccctcg atgtaaggga tgatcggctg atcggaaacg ttgagcgaga aatccttgtt gacggtgatc ttgtcaccgc cttccggaac cttgatgtgc tgatacggca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMP YQHIKVPEGG DKITVNKDFS LNVSDQPIIP YIEGDGTGFD ITPVMIKVVD AAVEKAYGGK KKIHWMEIYA GEKATKVYGP DVWLPEETLQ VLKEYVVSIK GPLTTPVGGG IRSLNVALRQ ELDLYVCLRP IQYFKGVPSP VREPEKTNMV IFRENSEDIY AGIEWAAESE QAKKVIKFLQ EEMGVKKIRF PQTSGIGIKP VSKEGTERLV RKAIQYAIDN DRKSVTLVHK GNIMKFTEGA FRDAGYALAQ KEFGAELIDG GPWMKFKNPK TGNEIVVKDS IADAFLQQIL LRPAEYDVIA TLNLNGDYIS DALAAQVGGI GIAPGANLSD SVAMFEATHG TAPKYAGKDY VNPGSEILSA EMMLRHLGWT EAADVIISAM EKSIKQKRVT YDFARLMEGA TQVSCSGFGQ VLIENME
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGT ATCAGCACAT CAAGGTTCCG GAAGGCGGTG ACAAGATCAC CGTCAACAAG GATTTCTCGC TCAACGTTTC CGATCAGCCG ATCATCCCTT ACATCGAGGG CGACGGCACG GGCTTCGACA TCACGCCGGT GATGATCAAG GTCGTCGACG CGGCGGTCGA GAAGGCGTAC GGCGGCAAGA AGAAGATTCA CTGGATGGAG ATCTACGCCG GCGAGAAGGC GACGAAGGTC TACGGCCCGG ACGTGTGGCT GCCGGAAGAG ACGCTGCAGG TGCTGAAGGA ATACGTCGTG TCGATCAAGG GGCCGCTCAC GACCCCGGTC GGCGGCGGCA TCCGCTCGCT GAACGTCGCG CTGCGTCAGG AGCTCGACCT GTACGTCTGC CTGCGCCCGA TCCAGTACTT CAAGGGCGTG CCTTCGCCCG TGCGCGAGCC GGAGAAGACC AACATGGTCA TCTTCCGCGA GAACTCGGAA GACATCTACG CCGGCATCGA ATGGGCGGCC GAATCCGAGC AGGCGAAGAA GGTCATCAAG TTCCTGCAGG AAGAGATGGG CGTGAAGAAG ATCCGCTTCC CGCAGACCTC GGGCATCGGC ATCAAGCCCG TGTCGAAGGA AGGCACCGAG CGCCTCGTGC GCAAGGCGAT CCAGTACGCG ATCGACAACG ACCGCAAGTC GGTCACGCTC GTCCACAAGG GCAACATCAT GAAGTTCACG GAAGGTGCGT TCCGTGACGC CGGCTATGCG CTCGCGCAGA AGGAATTCGG CGCCGAGCTG ATCGACGGCG GCCCGTGGAT GAAGTTCAAG AACCCGAAGA CGGGCAACGA GATCGTGGTC AAGGATTCGA TCGCCGACGC GTTCCTGCAG CAGATCCTGC TGCGCCCGGC CGAATACGAC GTGATCGCGA CGCTGAACCT GAACGGCGAC TACATCTCGG ACGCGCTCGC CGCGCAAGTC GGCGGCATCG GCATCGCGCC GGGCGCGAAC CTGTCGGATT CGGTCGCGAT GTTCGAAGCG ACGCACGGCA CGGCGCCGAA GTACGCGGGC AAGGATTACG TGAACCCGGG TTCGGAAATC CTGTCGGCCG AAATGATGCT GCGCCACCTC GGCTGGACCG AAGCGGCCGA CGTGATCATT TCCGCGATGG AGAAGTCGAT CAAGCAGAAG CGCGTCACGT ACGACTTCGC GCGCCTGATG GAAGGCGCGA CGCAAGTGTC GTGCTCGGGC TTCGGTCAGG TGCTGATCGA AAACATGGAG TAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT