BupsA.00092.a
isocitrate dehydrogenase
CENTER ID:
BupsA.00092.a
ORGANISM:
Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:
Melioidosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
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Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BupsA.00092.a.A1.PW25020
Full length( BupsA.00092.a )
info
1
419
PW25020
BupsA.00092.a.A1.PW25104
Full length( BupsA.00092.a )
info
1
419
PW25104
BupsA.00092.a.B1.PW25188
Full length( BupsA.00092.a )
info
1
419
PW25188
Structures
3DMS
DEPOSITED: 7/1/2008
DETERMINATION: XRay
CLONE: BupsA.00092.a.B1.GE30977
PROTEIN: BupsA.00092.a.B1.PS00006
Publications by SSGCID
Structural basis of the substrate specificity of bifunctional isocitrate dehydrogenase kinase/phosphatase.
Bryan CM, Edwards TE, Jia Z, Myler PJ, Stein AJ, Stewart LJ, Van Voorhis WC, Yates SP, Zheng J
Biochemistry - 2011
volume 50, issue 38, pages 8103-6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPYQHIKVPE GGDKITVNKD FSLNVSDQPI IPYIEGDGTG FDITPVMIKV VDAAVEKAYG GKKKIHWMEI YAGEKATKVY GPDVWLPEET LQVLKEYVVS IKGPLTTPVG GGIRSLNVAL RQELDLYVCL RPIQYFKGVP SPVREPEKTN MVIFRENSED IYAGIEWAAE SEQAKKVIKF LQEEMGVKKI RFPQTSGIGI KPVSKEGTER LVRKAIQYAI DNDRKSVTLV HKGNIMKFTE GAFRDAGYAL AQKEFGAELI DGGPWMKFKN PKTGNEIVVK DSIADAFLQQ ILLRPAEYDV IATLNLNGDY ISDALAAQVG GIGIAPGANL SDSVAMFEAT HGTAPKYAGK DYVNPGSEIL SAEMMLRHLG WTEAADVIIS AMEKSIKQKR VTYDFARLME GATQVSCSGF GQVLIENME
NT Sequence
ATGCCGTATC AGCACATCAA GGTTCCGGAA GGCGGTGACA AGATCACCGT CAACAAGGAT TTCTCGCTCA ACGTTTCCGA TCAGCCGATC ATCCCTTACA TCGAGGGCGA CGGCACGGGC TTCGACATCA CGCCGGTGAT GATCAAGGTC GTCGACGCGG CGGTCGAGAA GGCGTACGGC GGCAAGAAGA AGATTCACTG GATGGAGATC TACGCCGGCG AGAAGGCGAC GAAGGTCTAC GGCCCGGACG TGTGGCTGCC GGAAGAGACG CTGCAGGTGC TGAAGGAATA CGTCGTGTCG ATCAAGGGGC CGCTCACGAC CCCGGTCGGC GGCGGCATCC GCTCGCTGAA CGTCGCGCTG CGTCAGGAGC TCGACCTGTA CGTCTGCCTG CGCCCGATCC AGTACTTCAA GGGCGTGCCT TCGCCCGTGC GCGAGCCGGA GAAGACCAAC ATGGTCATCT TCCGCGAGAA CTCGGAAGAC ATCTACGCCG GCATCGAATG GGCGGCCGAA TCCGAGCAGG CGAAGAAGGT CATCAAGTTC CTGCAGGAAG AGATGGGCGT GAAGAAGATC CGCTTCCCGC AGACCTCGGG CATCGGCATC AAGCCCGTGT CGAAGGAAGG CACCGAGCGC CTCGTGCGCA AGGCGATCCA GTACGCGATC GACAACGACC GCAAGTCGGT CACGCTCGTC CACAAGGGCA ACATCATGAA GTTCACGGAA GGTGCGTTCC GTGACGCCGG CTATGCGCTC GCGCAGAAGG AATTCGGCGC CGAGCTGATC GACGGCGGCC CGTGGATGAA GTTCAAGAAC CCGAAGACGG GCAACGAGAT CGTGGTCAAG GATTCGATCG CCGACGCGTT CCTGCAGCAG ATCCTGCTGC GCCCGGCCGA ATACGACGTG ATCGCGACGC TGAACCTGAA CGGCGACTAC ATCTCGGACG CGCTCGCCGC GCAAGTCGGC GGCATCGGCA TCGCGCCGGG CGCGAACCTG TCGGATTCGG TCGCGATGTT CGAAGCGACG CACGGCACGG CGCCGAAGTA CGCGGGCAAG GATTACGTGA ACCCGGGTTC GGAAATCCTG TCGGCCGAAA TGATGCTGCG CCACCTCGGC TGGACCGAAG CGGCCGACGT GATCATTTCC GCGATGGAGA AGTCGATCAA GCAGAAGCGC GTCACGTACG ACTTCGCGCG CCTGATGGAA GGCGCGACGC AAGTGTCGTG CTCGGGCTTC GGTCAGGTGC TGATCGAAAA CATGGAG
Details for BupsA.00092.a.A1.PW25020
PURIFICATION DATe:
4/7/2008
CONCENTRATION:
29mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
20
VIAL VOLUME:
50µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for BupsA.00092.a.A1.PW25104
PURIFICATION DATe:
3/21/2008
CONCENTRATION:
data unavailable
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
58
VIAL VOLUME:
50µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for BupsA.00092.a.B1.PW25188
PURIFICATION DATe:
10/18/2010
CONCENTRATION:
20.91mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
12
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100