BupsA.00089.e

Putative ABC transport system, exported substrate-binding protein

CENTER ID: BupsA.00089.e
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00089.e.A1.GE30888 Full length( BupsA.00089.e ) 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.5701
RefSeq: YP_336738.1
UniProt: Q3JI65

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGISSNHGS FGIARYRRRC AACVRRVYER LRHQLLLGWC RHDHLAYVHL QRIEDRNYAK GKCYLDSRHW TFVDSTTDGL GSRQEGCRTD CYAGEIRAGS REHSNDRNRM KKVVGAMLLL AVGHAVSAEL HIGSWPDYLP DTLIKKFQAE TGIKTTLDTY ASDAALTQKL QSGGGGYDVV IAGDYYVPVL VKSGLLQKLD KNKLPNIANI KPEYRHPSFD PQRDYAMPYT VVLTGFAYDS ARVSGGKLDE SWKSFFDPPA QLRGQIGDLD VEEELYMAAS WYLGQDECTE NPADAKRVLD VLQKQKPFVK TYSNDGTIDR LASKQIAVQH IWSGAAARAQ DRLPSITFVY PREGVRLFMD SLLIPAKAQN TASAYQFVNW MMRPENIAQV TNAVRYNNEI IGSERYIDAA LLKNSAIKTP EQYKIRLRPY KICSPAAIQL RNKVWLKLKG NR
NT Sequence
ATGGGCGGCA TTTCGTCAAA TCACGGTTCC TTTGGCATTG CCCGGTATCG TCGCCGGTGC GCTGCTTGCG TTCGTCGCGT CTATGAACGA CTACGTCACC AGCTACTTCT TGGCTGGTGC CGGCATGACC ACCTTGCCTA TGTACATCTT CAGCGCATTG AAGATCGGAA TTACGCCAAA GGTAAATGCT ATCTCGACAG CCGTCATTGG ACTTTCGTCG ATTCAACTAC TGACGGTCTG GGTTCTCGGC AGGAAGGGTG CAGGACAGAC TGCTACGCCG GGGAGATCCG AGCTGGGTCA CGCGAACATT CAAACGATAG GAACCGTATG AAAAAAGTAG TCGGAGCGAT GCTGCTGCTT GCTGTCGGTC ATGCTGTCTC AGCCGAACTC CATATCGGAA GCTGGCCGGA TTACCTGCCC GATACGCTGA TCAAGAAGTT CCAGGCGGAA ACCGGCATCA AGACAACGCT CGACACCTAC GCGAGCGATG CGGCCCTCAC TCAAAAGTTG CAGTCGGGCG GAGGTGGCTA CGACGTTGTG ATCGCAGGGG ACTATTACGT TCCGGTGCTT GTGAAGTCTG GATTGCTGCA GAAGCTCGAC AAGAACAAGC TGCCGAATAT CGCCAACATC AAACCCGAGT ATCGCCATCC GTCATTCGAT CCGCAACGGG ATTATGCGAT GCCATACACC GTCGTGCTGA CTGGTTTCGC GTACGATAGC GCGCGCGTTT CCGGAGGGAA ACTCGACGAG AGTTGGAAGT CGTTCTTCGA TCCGCCAGCG CAGTTGCGCG GACAGATCGG CGACCTGGAT GTCGAAGAGG AGCTGTATAT GGCCGCGAGT TGGTATCTGG GACAGGATGA ATGCACAGAA AATCCTGCTG ATGCGAAGCG GGTGCTCGAC GTTTTGCAAA AACAGAAACC GTTCGTGAAA ACGTACAGCA ATGACGGAAC CATAGATCGT CTCGCGTCCA AGCAGATCGC GGTGCAGCAT ATTTGGAGCG GTGCCGCTGC GCGTGCACAG GACCGTCTTC CCAGCATCAC CTTTGTATAC CCGAGGGAAG GCGTCCGTCT GTTCATGGAT AGCTTGCTTA TTCCGGCAAA GGCACAAAAC ACCGCCTCGG CATATCAATT CGTTAACTGG ATGATGCGCC CGGAGAACAT TGCGCAGGTT ACAAATGCCG TTCGCTACAA CAACGAGATC ATCGGCTCCG AGCGGTATAT CGATGCCGCA CTGTTGAAAA ACTCCGCGAT AAAAACCCCG GAGCAATACA AAATAAGGCT CCGTCCTTAC AAAATATGTT CGCCGGCGGC GATTCAGTTA CGAAACAAAG TCTGGCTAAA GTTGAAGGGA AATCGC
Details for BupsA.00089.e.A1.GE30888
HARVESTED ON: 11/23/2010
SEQUENCED ON: 12/6/2010
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 53
Validated AA Sequence
MXXXXXXTXX GXXXXISSNH GSFGIARXRR RCAACVRRVY XXXRHQLLLG WCRHDHLAYV HLQRIEDRNY AKGKCYLDSR HWTFVDSTTD GLGSRQEGCR TDCYAGEIRA GSREHSNDRN RMKKVVGAML LLAVGHAVSA ELHIGSWPDY LPDTLIKKFQ AETGIKTTLD TYASDAALTQ KLQSGGGGYX VVIAGDYYVP XLVKSGLLXK LDKNKLXNIX XIXXEYRHPS FDPQRXXCXA IHRRADWXXV R
Validated NT Sequence
antnctnnnn nnggnganat ncngnnnnnt nnnnatnncn ntcntatggn anccnngnnn gntgnnaccg ntnnnggnnn nnnnnnnngc atttcgtcaa atcacggttc ctttggcatt gccaggnatc gtcgccggtg cgctgcttgc gttcgtcgcg tctatgancn antacgtcac cagctacttc ttggctggtg ccggcatgac caccttgcct atgtacatct tcagcgcatt gaagatcgga attacgccaa aggtaaatgc tatctcgaca gccgtcattg gactttcgtc gattcaacta ctgacggtct gggttctcgg caggaagggt gcaggacaga ctgctacgcc ggggagatcc gagctgggtc acgcgaacat tcaaacgata ggaaccgtat gaaaaaagta gtcggagcga tgctgctgct tgctgtcggt catgctgtct cagccgaact ccatatcgga agctggccgg attacctgcc cgatacgctg atcaagaagt tccaggcgga aaccggcatc aagacaacgc tcgacaccta cgcgagcgat gcggccctca ctcaaaagtt gcagtcnggc ggaggtggct acnacgttgt gatcgcaggg gactattacg ttccngngct tgtgaagtct ggattgctgc anaagctcga caagaacaag ctgcngaata tcnccaanat caancnngag tatcgccatc cgtcattcga tccgcaacgn nnantatgcg angccataca ccgncgtgct gactggnttc ncgtacgata gcgngcgcgn nnncngagga aactcgacna nanntggnaa gtcgttcttc natccnnnng cncagttngc ncgnacnnga t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGGISSNHG SFGIARYRRR CAACVRRVYE RLRHQLLLGW CRHDHLAYVH LQRIEDRNYA KGKCYLDSRH WTFVDSTTDG LGSRQEGCRT DCYAGEIRAG SREHSNDRNR MKKVVGAMLL LAVGHAVSAE LHIGSWPDYL PDTLIKKFQA ETGIKTTLDT YASDAALTQK LQSGGGGYDV VIAGDYYVPV LVKSGLLQKL DKNKLPNIAN IKPEYRHPSF DPQRDYAMPY TVVLTGFAYD SARVSGGKLD ESWKSFFDPP AQLRGQIGDL DVEEELYMAA SWYLGQDECT ENPADAKRVL DVLQKQKPFV KTYSNDGTID RLASKQIAVQ HIWSGAAARA QDRLPSITFV YPREGVRLFM DSLLIPAKAQ NTASAYQFVN WMMRPENIAQ VTNAVRYNNE IIGSERYIDA ALLKNSAIKT PEQYKIRLRP YKICSPAAIQ LRNKVWLKLK GNR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcggcatt tcgtcaaatc acggttcctt tggcattgcc cggtatcgtc gccggtgcgc tgcttgcgtt cgtcgcgtct atgaacgact acgtcaccag ctacttcttg gctggtgccg gcatgaccac cttgcctatg tacatcttca gcgcattgaa gatcggaatt acgccaaagg taaatgctat ctcgacagcc gtcattggac tttcgtcgat tcaactactg acggtctggg ttctcggcag gaagggtgca ggacagactg ctacgccggg gagatccgag ctgggtcacg cgaacattca aacgatagga accgtatgaa aaaagtagtc ggagcgatgc tgctgcttgc tgtcggtcat gctgtctcag ccgaactcca tatcggaagc tggccggatt acctgcccga tacgctgatc aagaagttcc aggcggaaac cggcatcaag acaacgctcg acacctacgc gagcgatgcg gccctcactc aaaagttgca gtcgggcgga ggtggctacg acgttgtgat cgcaggggac tattacgttc cggtgcttgt gaagtctgga ttgctgcaga agctcgacaa gaacaagctg ccgaatatcg ccaacatcaa acccgagtat cgccatccgt cattcgatcc gcaacgggat tatgcgatgc catacaccgt cgtgctgact ggtttcgcgt acgatagcgc gcgcgtttcc ggagggaaac tcgacgagag ttggaagtcg ttcttcgatc cgccagcgca gttgcgcgga cagatcggcg acctggatgt cgaagaggag ctgtatatgg ccgcgagttg gtatctggga caggatgaat gcacagaaaa tcctgctgat gcgaagcggg tgctcgacgt tttgcaaaaa cagaaaccgt tcgtgaaaac gtacagcaat gacggaacca tagatcgtct cgcgtccaag cagatcgcgg tgcagcatat ttggagcggt gccgctgcgc gtgcacagga ccgtcttccc agcatcacct ttgtataccc gagggaaggc gtccgtctgt tcatggatag cttgcttatt ccggcaaagg cacaaaacac cgcctcggca tatcaattcg ttaactggat gatgcgcccg gagaacattg cgcaggttac aaatgccgtt cgctacaaca acgagatcat cggctccgag cggtatatcg atgccgcact gttgaaaaac tccgcgataa aaaccccgga gcaatacaaa ataaggctcc gtccttacaa aatatgttcg ccggcggcga ttcagttacg aaacaaagtc tggctaaagt tgaagggaaa tcgctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa