SSGCID: Target Page

BupsA.00089.c

ABC transporter, substrate binding protein (Polyamine)

CENTER ID:	BupsA.00089.c
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00089.c.A2.GE32531	Mature protein( BupsA.00089.c )		27	343

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00089.c.A2.PS01139	Mature protein( BupsA.00089.c )		27	343

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.2305
RefSeq:	YP_333606.1
UniProt:	Q3JS47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNSKSVFRQA TLMAVCATAF AAAGAQAADS IVFTSWGGTT QSSQDKDWAK PFTKATGINV MMDGPTDYGK LKAMVDSGNV TWDVVDVEGD FAYAAQKAGL IEPIDYSVVK KDELDPRFVS PAAVGSFYYA FVLGFNKASF KGAQPSTWAD LFDTKRFPGK RTFYKWSAPG VLEIALLADG VPVNKLYPLD LDRAFKKLDS IKSDIVWWGG GAQSQQLLAS GEAPIGMFWN GRLHALEQTG VPVGISWTQN LTAADMLVIP KGAKHRAEAM KYLAAVTSAD AQAKFAADTG YAPINMKSPS MMPAAVAKTL PDQYKSSQID LDMKYWADHR DEIAKRWYAW QSK

NT Sequence

ATGAACAGCA AGAGCGTTTT TCGTCAGGCG ACGCTGATGG CGGTGTGCGC GACCGCGTTT GCGGCCGCCG GCGCACAAGC GGCCGATTCG ATTGTGTTCA CGAGTTGGGG CGGCACGACG CAATCGTCGC AGGACAAGGA TTGGGCCAAG CCCTTCACGA AGGCGACGGG CATCAACGTG ATGATGGACG GCCCGACCGA CTACGGCAAG CTCAAGGCGA TGGTCGATAG CGGCAACGTG ACCTGGGACG TCGTCGACGT CGAAGGCGAT TTCGCTTATG CCGCGCAAAA GGCCGGACTC ATCGAGCCGA TCGATTATTC GGTCGTCAAG AAGGACGAAC TAGATCCGCG TTTCGTGTCG CCCGCGGCGG TGGGCAGCTT CTATTACGCT TTCGTGCTCG GTTTCAACAA AGCGTCGTTC AAGGGGGCGC AGCCGTCGAC CTGGGCCGAC CTCTTCGACA CCAAGCGCTT CCCCGGCAAG CGCACCTTCT ATAAGTGGTC GGCGCCCGGC GTGCTCGAAA TCGCGCTGCT GGCCGATGGC GTGCCGGTGA ACAAACTCTA TCCGCTCGAT CTCGATCGCG CGTTCAAGAA GCTCGACTCG ATCAAGAGCG ACATCGTCTG GTGGGGCGGC GGCGCCCAGT CGCAGCAGTT GCTCGCATCG GGCGAGGCGC CGATCGGCAT GTTCTGGAAC GGACGTCTGC ACGCGCTCGA ACAGACGGGC GTTCCGGTCG GCATCTCCTG GACGCAAAAC CTGACGGCCG CTGACATGCT CGTGATTCCG AAGGGCGCGA AACACCGGGC CGAAGCGATG AAGTACCTCG CCGCTGTCAC CAGCGCCGAT GCGCAAGCGA AATTCGCCGC CGACACGGGA TACGCGCCGA TCAATATGAA ATCGCCTTCG ATGATGCCTG CTGCCGTGGC CAAGACGCTG CCCGATCAGT ACAAGTCGTC GCAGATCGAT CTCGACATGA AGTACTGGGC GGATCATCGC GACGAGATCG CGAAGCGCTG GTACGCCTGG CAATCGAAG

Details for BupsA.00089.c.A2.GE32531

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/24/2011
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMAADSIV FTSWGGTTQS SQDKDWAKPF TKATGINVMM DGPTDYGKLK AMVDSGNVTW DVVDVEGDFA YAAQKAGLIE PIDYSVVKKD ELDPRFVSPA AVGSFYYAFV LGFNKASFKG AQPSTWADLF DTKRFPGKRT FYKWSAPGVL EIALLADGVP VNKLYPLDLD RAFKKLDSIK SDIVWWGGGA QSQQLLASGE APIGMFWNGR LHALEQTGVP VGISWTQNLT AADMLVIPKG AKHRAEAMKY LAAVTSADAQ AKFAADTGYA PINMKSPSMM PAAVAKTLPD QYKSSQIDLD MKYWADHRDE IAKRWYAWQS K

Validated NT Sequence

acttcgattg ccaggcgtac cagcgcttcg cgatctcgtc gcgatgatcc gcccagtact tcatgtcgag atcgatctgc gacgacttgt actgatcggg cagcgtcttg gccacggcag caggcatcat cgaaggcgat ttcatattga tcggcgcgta tcccgtgtcg gcggcgaatt tcgcttgcgc atcggcgctg gtgacagcgg cgaggtactt catcgcttcg gcccggtgtt tcgcgccctt cggaatcacg agcatgtcag cggccgtcag gttttgcgtc caggagatgc cgaccggaac gcccgtctgt tcgagcgcgt gcagacgtcc gttccagaac atgccgatcg gcgcctcgcc cgatgcgagc aactgctgcg actgggcgcc gccgccccac cagacgatgt cgctcttgat cgagtcgagc ttcttgaacg cgcgatcgag atcgagcgga tagagtttgt tcaccggcac gccatcggcc agcagcgcga tttcgagcac gccgggcgcc gaccacttat agaaggtgcg cttgccgggg aagcgcttgg tgtcgaagag gtcggcccag gtcgacggct gcgccccctt gaacgacgct ttgttgaaac cgagcacgaa agcgtaatag aagctgccca ccgccgcggg cgacacgaaa cgcggatcta gttcgtcctt cttgacgacc gaataatcga tcggctcgat gagtccggcc ttttgcgcgg cataagcgaa atcgccttcg acgtcgacga cgtcccaggt cacgttgccg ctatcgacca tcgccttgag cttgccgtag tcggtcgggc cgtccatcat cacgttgatg cccgtcgcct tcgtgaaggg cttggcccaa tccttgtcct gcgacgattg cgtcgtgccg ccccaactcg tgaacacaat cgaatcggcc gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAADSIVFT SWGGTTQSSQ DKDWAKPFTK ATGINVMMDG PTDYGKLKAM VDSGNVTWDV VDVEGDFAYA AQKAGLIEPI DYSVVKKDEL DPRFVSPAAV GSFYYAFVLG FNKASFKGAQ PSTWADLFDT KRFPGKRTFY KWSAPGVLEI ALLADGVPVN KLYPLDLDRA FKKLDSIKSD IVWWGGGAQS QQLLASGEAP IGMFWNGRLH ALEQTGVPVG ISWTQNLTAA DMLVIPKGAK HRAEAMKYLA AVTSADAQAK FAADTGYAPI NMKSPSMMPA AVAKTLPDQY KSSQIDLDMK YWADHRDEIA KRWYAWQSK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccgat tcgattgtgt tcacgagttg gggcggcacg acgcaatcgt cgcaggacaa ggattgggcc aagcccttca cgaaggcgac gggcatcaac gtgatgatgg acggcccgac cgactacggc aagctcaagg cgatggtcga tagcggcaac gtgacctggg acgtcgtcga cgtcgaaggc gatttcgctt atgccgcgca aaaggccgga ctcatcgagc cgatcgatta ttcggtcgtc aagaaggacg aactagatcc gcgtttcgtg tcgcccgcgg cggtgggcag cttctattac gctttcgtgc tcggtttcaa caaagcgtcg ttcaaggggg cgcagccgtc gacctgggcc gacctcttcg acaccaagcg cttccccggc aagcgcacct tctataagtg gtcggcgccc ggcgtgctcg aaatcgcgct gctggccgat ggcgtgccgg tgaacaaact ctatccgctc gatctcgatc gcgcgttcaa gaagctcgac tcgatcaaga gcgacatcgt ctggtggggc ggcggcgccc agtcgcagca gttgctcgca tcgggcgagg cgccgatcgg catgttctgg aacggacgtc tgcacgcgct cgaacagacg ggcgttccgg tcggcatctc ctggacgcaa aacctgacgg ccgctgacat gctcgtgatt ccgaagggcg cgaaacaccg ggccgaagcg atgaagtacc tcgccgctgt caccagcgcc gatgcgcaag cgaaattcgc cgccgacacg ggatacgcgc cgatcaatat gaaatcgcct tcgatgatgc ctgctgccgt ggccaagacg ctgcccgatc agtacaagtc gtcgcagatc gatctcgaca tgaagtactg ggcggatcat cgcgacgaga tcgcgaagcg ctggtacgcc tggcaatcga agtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BupsA.00089.c.A2.PS01139

PURIFICATION DATe:	6/28/2011
CONCENTRATION:	31.04mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCCGAT TCGATTGTGT TCACGAGTTG GGGCGGCACG ACGCAATCGT CGCAGGACAA GGATTGGGCC AAGCCCTTCA CGAAGGCGAC GGGCATCAAC GTGATGATGG ACGGCCCGAC CGACTACGGC AAGCTCAAGG CGATGGTCGA TAGCGGCAAC GTGACCTGGG ACGTCGTCGA CGTCGAAGGC GATTTCGCTT ATGCCGCGCA AAAGGCCGGA CTCATCGAGC CGATCGATTA TTCGGTCGTC AAGAAGGACG AACTAGATCC GCGTTTCGTG TCGCCCGCGG CGGTGGGCAG CTTCTATTAC GCTTTCGTGC TCGGTTTCAA CAAAGCGTCG TTCAAGGGGG CGCAGCCGTC GACCTGGGCC GACCTCTTCG ACACCAAGCG CTTCCCCGGC AAGCGCACCT TCTATAAGTG GTCGGCGCCC GGCGTGCTCG AAATCGCGCT GCTGGCCGAT GGCGTGCCGG TGAACAAACT CTATCCGCTC GATCTCGATC GCGCGTTCAA GAAGCTCGAC TCGATCAAGA GCGACATCGT CTGGTGGGGC GGCGGCGCCC AGTCGCAGCA GTTGCTCGCA TCGGGCGAGG CGCCGATCGG CATGTTCTGG AACGGACGTC TGCACGCGCT CGAACAGACG GGCGTTCCGG TCGGCATCTC CTGGACGCAA AACCTGACGG CCGCTGACAT GCTCGTGATT CCGAAGGGCG CGAAACACCG GGCCGAAGCG ATGAAGTACC TCGCCGCTGT CACCAGCGCC GATGCGCAAG CGAAATTCGC CGCCGACACG GGATACGCGC CGATCAATAT GAAATCGCCT TCGATGATGC CTGCTGCCGT GGCCAAGACG CTGCCCGATC AGTACAAGTC GTCGCAGATC GATCTCGACA TGAAGTACTG GGCGGATCAT CGCGACGAGA TCGCGAAGCG CTGGTACGCC TGGCAATCGA AGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA