BupsA.00089.b

ABC transporter, periplasmic substrate-binding protein

CENTER ID: BupsA.00089.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00089.b.A2.GE30885 Mature protein( BupsA.00089.b ) 21 364
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.333
RefSeq: YP_331747.1
UniProt: Q3JXF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRIAWLAAV LASLACAAAR AADGNVLNIY NWAEYFAPDT IADFEKETGI KVRLDVYDSN EALQTKLTTG NSGYDLVFPS NDFLARQIQA GLYRKLDKSR LPNLTNLDPA IVARAAEVDP GNQYSVPYMQ GTFGLGLNVA KVKQALGGPL PANTLELIFN PAYAAKLERC GIAFNDAGSE VFPLALRYIG RDPNTTDPRD YEAALDMMKT IRPTIRQFIA TPVMNDLATG DVCVVTGYSG AVLVAARRAA EAKNGQQIVY SLPSAGAPFW FDSMAIPKGA AHADHALRFI DYILRPDVVA KISNKVMYPN PNRVATPLVD RRLTANPAIY PDAATMRTLW VKRPMPPQAM RMQTRYWTRF KTGY
NT Sequence
ATGAAGCGCA TCGCGTGGCT CGCCGCCGTG CTCGCATCGC TCGCGTGCGC CGCGGCGCGC GCGGCCGACG GCAACGTGCT CAACATCTAC AATTGGGCCG AGTATTTCGC GCCCGACACG ATCGCCGACT TCGAGAAGGA AACCGGCATC AAGGTGCGGC TCGACGTGTA CGACAGCAAC GAAGCGCTGC AAACGAAGCT GACGACGGGC AACAGCGGCT ACGATCTCGT GTTTCCGTCA AACGATTTTC TCGCACGGCA GATTCAGGCG GGCCTCTACC GGAAGCTCGA CAAATCGAGG CTGCCGAACC TCACGAACCT CGATCCGGCG ATCGTCGCGC GCGCCGCCGA AGTCGATCCC GGCAACCAGT ACAGCGTGCC GTACATGCAA GGCACGTTCG GGCTCGGGCT GAATGTCGCG AAGGTGAAGC AGGCGCTCGG CGGCCCGCTG CCCGCGAACA CGCTCGAGCT GATCTTCAAT CCTGCGTATG CGGCGAAGCT CGAGCGCTGC GGCATCGCGT TCAACGATGC GGGCAGCGAA GTGTTTCCGC TCGCGCTGCG CTACATCGGC CGCGATCCGA ACACGACCGA TCCGCGCGAC TATGAGGCGG CGCTCGACAT GATGAAGACG ATCCGGCCGA CGATCCGCCA GTTCATCGCG ACGCCCGTGA TGAACGATCT CGCGACGGGC GACGTATGCG TCGTCACCGG CTACTCGGGC GCGGTGCTTG TCGCGGCGCG GCGCGCGGCC GAGGCGAAGA ACGGGCAGCA GATCGTCTAC AGCCTGCCCT CGGCCGGCGC GCCGTTCTGG TTCGACAGCA TGGCGATTCC GAAAGGTGCG GCGCACGCGG ACCATGCGCT GCGCTTCATC GATTACATTC TGAGGCCCGA CGTCGTCGCG AAGATCAGCA ACAAGGTGAT GTACCCGAAT CCGAACCGCG TGGCGACGCC GCTCGTCGAC CGGCGGCTCA CGGCCAACCC GGCGATCTAT CCGGATGCGG CGACGATGCG TACGCTGTGG GTGAAGCGCC CGATGCCGCC GCAGGCAATG CGCATGCAGA CGCGCTACTG GACCCGGTTC AAGACCGGTT AC
Details for BupsA.00089.b.A2.GE30885
HARVESTED ON: 11/23/2010
SEQUENCED ON: 12/6/2010
EXPECTED MW: 40kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 87
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAADGNEAL QTKLTTGNSG YDLVFPSNDF LARQIQAGLY RKLDKSRLPN LTNLDPAIVA RAAEVDPGNQ YSVPYMQGTF GLGLNVAKVK QALGGPLPAN TLELIFNPAY AAKLERCGIA FNDAGSEVFP LALRYIGRDP NTTDPRDYEA ALDMMKTIRP TIRQFIATPV MNDLATGDVC VVTGYSGAVL VAARRAAERR TGSRSSTACP RPARRSGSTA WRFRKVRRTX XMRCASSXTF
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg ccgacggcaa cgaagcgctg caaacgaagc tgacgacggg caacagcggc tacgatctcg tgtttccgtc aaacgatttt ctcgcacggc agattcaggc gggcctctac cggaagctcg acaaatcgag gctgccgaac ctcacgaacc tcgatccggc gatcgtcgcg cgcgccgccg aagtcgatcc cggcaaccag tacagcgtgc cgtacatgca aggcacgttc gggctcgggc tgaatgtcgc gaaggtgaag caggcgctcg gcggcccgct gcccgcgaac acgctcgagc tgatcttcaa tcctgcgtat gcggcgaagc tcgagcgctg cggcatcgcg ttcaacgatg cgggcagcga agtgtttccg ctcgcgctgc gctacatcgg ccgcgatccg aacacgaccg atccgcgcga ctatgaggcg gcgctcgaca tgatgaagac gatccggccg acgatccgcc agttcatcgc gacgcccgtg atgaacgatc tcgcgacggg cgacgtatgc gtcgtcaccg gctactcggg cgcggtgctt gtcgcggcgc ggcgcgcggc cgagcgaaga acgggcagca gatcgtctac agcctgccct cggccggcgc gccgttctgg ttcgacagca tggcgattcc gaaaggtgcg gcgcacgcng ancatgcgct gcgcttcatc gatnacattc tgaggcccga cgtcgtcncg aagatcagca acnaggtgat gtacccgaat ccnaaccgcg tggcgacgcc gctcgtcgan cggcngntca cggnnaaccc ngngatctat ncnggatgnn gcgacnatgc gtacgctgtn nngaagcnnc ncgatgncnc cncnnnnntg catgnngann nnnctactgg nnnngnnnnn nnnnnnntac nnnnaaacac naannnnttc ngcnnnnagn nnnnnnnngn nncnncngct nnnnaaacnc nnnnngnnnc tgannnngnn nnnnnnnnnn nananna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAADGNVLN IYNWAEYFAP DTIADFEKET GIKVRLDVYD SNEALQTKLT TGNSGYDLVF PSNDFLARQI QAGLYRKLDK SRLPNLTNLD PAIVARAAEV DPGNQYSVPY MQGTFGLGLN VAKVKQALGG PLPANTLELI FNPAYAAKLE RCGIAFNDAG SEVFPLALRY IGRDPNTTDP RDYEAALDMM KTIRPTIRQF IATPVMNDLA TGDVCVVTGY SGAVLVAARR AAEAKNGQQI VYSLPSAGAP FWFDSMAIPK GAAHADHALR FIDYILRPDV VAKISNKVMY PNPNRVATPL VDRRLTANPA IYPDAATMRT LWVKRPMPPQ AMRMQTRYWT RFKTGY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccgac ggcaacgtgc tcaacatcta caattgggcc gagtatttcg cgcccgacac gatcgccgac ttcgagaagg aaaccggcat caaggtgcgg ctcgacgtgt acgacagcaa cgaagcgctg caaacgaagc tgacgacggg caacagcggc tacgatctcg tgtttccgtc aaacgatttt ctcgcacggc agattcaggc gggcctctac cggaagctcg acaaatcgag gctgccgaac ctcacgaacc tcgatccggc gatcgtcgcg cgcgccgccg aagtcgatcc cggcaaccag tacagcgtgc cgtacatgca aggcacgttc gggctcgggc tgaatgtcgc gaaggtgaag caggcgctcg gcggcccgct gcccgcgaac acgctcgagc tgatcttcaa tcctgcgtat gcggcgaagc tcgagcgctg cggcatcgcg ttcaacgatg cgggcagcga agtgtttccg ctcgcgctgc gctacatcgg ccgcgatccg aacacgaccg atccgcgcga ctatgaggcg gcgctcgaca tgatgaagac gatccggccg acgatccgcc agttcatcgc gacgcccgtg atgaacgatc tcgcgacggg cgacgtatgc gtcgtcaccg gctactcggg cgcggtgctt gtcgcggcgc ggcgcgcggc cgaggcgaag aacgggcagc agatcgtcta cagcctgccc tcggccggcg cgccgttctg gttcgacagc atggcgattc cgaaaggtgc ggcgcacgcg gaccatgcgc tgcgcttcat cgattacatt ctgaggcccg acgtcgtcgc gaagatcagc aacaaggtga tgtacccgaa tccgaaccgc gtggcgacgc cgctcgtcga ccggcggctc acggccaacc cggcgatcta tccggatgcg gcgacgatgc gtacgctgtg ggtgaagcgc ccgatgccgc cgcaggcaat gcgcatgcag acgcgctact ggacccggtt caagaccggt tactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa